| 质粒名称: | pLOVE-Nmyc |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 15951 |
| 存储实验室: | Miguel Ramalho-Santos |
|
载体骨架基本信息 |
|
| 载体名称: | pLOVE |
| 完整质粒大小: | 8263 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)/Mycn, Nmyc, N-myc |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 2500 bp |
| 基因突变: | The recombination reaction required to clone "Gene X" into the pLOVE vector backbone removes 1456 bp (the CmR-ccdB region). Therefore, the size of pLOVE-Nmyc is ~9307 bp. |
| 标签/融合蛋白: | V5 (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’测序引物 | n/a |
| 3’测序引物 | V5 |
|
文献引用方法 |
|
| 材料和方法部分: | pLOVE-Nmyc was a gift from Miguel Ramalho-Santos (Addgene plasmid # 15951 ; http://n2t.net/addgene:15951 ; RRID:Addgene_15951) |
| 参考文献部分: | Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection. Blelloch R, Venere M, Yen J, Ramalho-Santos M. Cell Stem Cell. 2007 Sep 13. ():. 10.1016/j.stem.2007.08.008 PubMed 18371358 |
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