| 质粒名称: | NM1-5S-tRNA-SgH |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 128178 |
| 存储实验室: | Huimin Zhao |
|
载体骨架基本信息 |
|
| 载体名称: | pZPK |
| 载体出品公司: | -- |
| 空载体大小: | 6303 bp |
| 完整质粒大小: | 9317 bp |
| 载体修饰: | 在LB和RB之间添加了弓形虫潮霉素抗性基因。在LB和潮霉素抗性基因之间添加R.toruloides 5S rRNA tRNA（Arg）启动子和polyT终止子。 |
| 载体类型: | 酵母表达，CRISPR |
| 筛选标记: | 潮霉素 |
| 载体抗性: | 卡那霉素，50μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | gRNA cloning cassette |
| 物种来源: | 圆红冬孢酵母菌 |
| 插入大小: | 951 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | 5S rRNA tRNA（精氨酸） |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BbsI（在克隆过程中被破坏） |
| 3’克隆位点: | BbsI（在克隆过程中被破坏） |
| 5’测序引物 | CAAATTGACGCTTAGACAAC |
| 3’测序引物 | TATATCCTGTCAAACACTGATAG |
|
文献引用方法 |
|
| 材料和方法部分: | NM1-5S-tRNA-SgH was a gift from Huimin Zhao (Addgene plasmid # 128178 ; http://n2t.net/addgene:128178 ; RRID:Addgene_128178) |
| 参考文献部分: | Development of a CRISPR/Cas9 system for high efficiency multiplexed gene deletion in Rhodosporidium toruloides. Schultz JC, Cao M, Zhao H. Biotechnol Bioeng. 2019 Apr 30. doi: 10.1002/bit.27001. 10.1002/bit.27001 PubMed 31038202 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS NM1-5S-tRNA-SgH 9382 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as
promoter.
ACCESSION .
VERSION .
KEYWORDS NM1-5S-tRNA-SgH
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9382)
AUTHORS Schultz JC, Cao M, Zhao H
TITLE Development of a CRISPR/Cas9 system for high efficiency multiplexed
gene deletion in Rhodosporidium toruloides.
JOURNAL Biotechnol Bioeng. 2019 Apr 30. doi: 10.1002/bit.27001.
PUBMED 31038202
REFERENCE 2 (bases 1 to 9382)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9382
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 47..71
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
misc_RNA 355..430
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
CDS 2896..2913
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
terminator 2917..3169
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 3270..3294
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 4607..5236
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 5665..6738
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGGAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 6804..6998
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(7234..7256)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 7342..7482
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 7397..7416
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(7497..7514)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7668..8256)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7748..7767)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind complement(8367..8386)
/label=pENTR-R
/note="pENTR vectors, reverse primer"
CDS 8378..9193
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(8451..8470)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
ORIGIN
1 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
61 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
121 actgaattaa cgccgaattg aattcatctg cggccatacc gcgatgaaca caccgcgtct
181 cgtccgatcc gcgaagttaa gcatcgcagg ggccagagag tattgccgtg ggtgaccagg
241 cgagaacact gtgctgccgc aggtggcggt gtggccaagt ggcacggcac ctgtttccgg
301 cgcaggagat cgagggttcg atccccttca ccgtcgaggt cttcaggaag acgagtttta
361 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
421 gagtcggtgc tttttttttg gtacccgggc tgcagaacta cgccctctca cacccaactt
481 ccgactcgac cggcggtacg agcacgacct acttctactg cctgccatcg acatccgggc
541 gggtcgctgc ctaccctgtg cgttctgcgc cctccctcgt ctcgggaggc agtgtctgac
601 agaagctttg cgcgcagtac cccgtcaaga tgcaactcta cgcaacgttc ggcacagaag
661 tcgccaagct ccgcgcatcg ccgcctcaag ctctcgcgct gcccgacggt gtcgtctatt
721 acgaggcgga gaagctcgag ttgccggctt tgccagcggc ggtcaaggtt gaggtggaga
781 cggagaaggc gggagtagcg ggggaggaca atgaggcgaa gggtgagatg gtgctggtgg
841 agactcttac ggtggagcag gaggagattg aattgggctc gggagtcgtg cagattgagg
901 agtcgttgct cgtcaagctg gaggtcagcg gctgatcctt ccgttcgttg caaggatcgt
961 ctgcatgttt cgcttctctc aatgacacaa cctggagagc gctcccgtca gcgagaatcg
1021 aggacattcc gcagctcgtg agcaagcgga ggtgcgaggc tccctcgaaa gctgcgcctc
1081 ttcagacggc ttgttctctc ctgctctggt gggctggcct gacatgtaat gtgctccgcc
1141 gcaagtccgt cgtcggtctc aattcgacgt tgaaagggca tagcgcaagg aagaaccctc
1201 tgcggacatg cagaattact ggctcgcctg ctccttcgtc tactggaata agtcctgtct
1261 cgttaaagcc ccaacgtcgt ttttcgacgt ttgtaaggcg caagaggtgc tatgggctac
1321 gcaggaagct gagaggacat agaagtcggg ggaggaacgg cgcagagcgg cagttgcgga
1381 agcatgagga aagcgagacg gtccagcatc tgcagcgcca atccgcaatc tcctggttga
1441 gcctgcaccg gaagcgtcgg aacagtatgc gcagagtcga acgcaagtaa gaaagacgca
1501 ccctcacact cgcttacttc gagccataca acggatcaaa gctgcgcgta tctcggcttg
1561 taagggccgg aaagcaacct cggagatgga cacgtcacat caccaactta tcgatctcgg
1621 ccgtcgacgt cgcagagagg gcgagagaag cggtgaagga gggaaacaac ccctcgagag
1681 catgatccga ccgaatctgc agcgcaggaa gccgttacaa gcccgcctcg agcgcaggtc
1741 gggtccagcc gggggacgaa acgcgcgagg ctgattcgtg agcgaaggaa gccgcatcga
1801 caagttcgct cccctttgcc ctctttccca tcacccgttc tcgccttacc cgctcagaac
1861 aacaccagat cactcacaat gccggagctc atggcgacgt cggtcgagaa gttcctcatc
1921 gaaaagttcg actcggtctc ggacctcatg caactctcgg agggagagga atcgcgcgcg
1981 ttctcgttcg acgtcggagg ccgcggatac gtcctccgcg tcaactcgtg tgcggacgga
2041 ttctacaagg atcggtacgt ctaccgccat tttgcgtcgg cggcgctccc gatccccgaa
2101 gtcctcgaca tcggagagtt ctcggaatcc ctcacgtact gtatctcgcg ccgggcgcaa
2161 ggagtcacgc tccaagatct cccggagacg gaactcccgg cggtcctcca accggtcgcg
2221 gaagcgatgg acgcgatcgc ggccgcggac ctctcgcaaa cgtcgggatt cggaccgttt
2281 ggaccgcaag gaatcggaca atacacgacg tggcgcgact tcatctgtgc gatcgcggat
2341 ccccatgtct accactggca aacggtcatg gatgacacgg tctcggcgtc ggtcgcgcaa
2401 gcgctcgacg agctcatgct ctgggcggag gactgtccgg aggtccgcca cctcgtccac
2461 gcggactttg gatcgaacaa cgtcctcacg gacaacggac gcatcacggc ggtcatcgac
2521 tggtcggagg cgatgtttgg agactcgcaa tacgaggtcg cgaacatctt cttctggcgc
2581 ccgtggctcg cgtgcatgga gcaacagacg cgctacttcg agcgccgcca cccggagctc
2641 gcgggatcgc cgcgcctccg cgcgtacatg ctccgcatcg gcctcgacca actctaccag
2701 tcgctcgtcg acggaaactt cgacgatgcc gcgtgggccc aaggacgctg cgacgcgatt
2761 gtccgctcgg gagcgggaac cgtgggacgc acgcaaattg cgcggcgctc ggcggccgtc
2821 tggacggatg gatgtgtcga agtcctcgcg gattcgggaa accggcgccc gtcgacgcgc
2881 ccgcgggcga aagaacacca ccatcaccat cactaggatc gttcaaacat ttggcaataa
2941 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
3001 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
3061 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
3121 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggcctggatc
3181 ctctagagtc gacctgcagc atgcaagctt ggcagcttga gcttggatca gattgtcgtt
3241 tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
3301 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt
3361 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttaaag
3421 tactttaaag tactttaaag tactttgatc caacccctcc gctgctatag tgcagtcggc
3481 ttctgacgtt cagtgcagcc gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
3541 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
3601 tagaaccgga gacattacgc catgaacaag agcgccaccg ctggcctgct gggctatgcc
3661 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
3721 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
3781 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
3841 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
3901 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
3961 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
4021 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
4081 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
4141 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
4201 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
4261 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
4321 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
4381 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
4441 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
4501 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
4561 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
4621 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
4681 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
4741 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
4801 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
4861 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
4921 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
4981 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
5041 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
5101 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
5161 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
5221 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
5281 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
5341 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
5401 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
5461 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
5521 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
5581 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
5641 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
5701 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
5761 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
5821 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
5881 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
5941 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
6001 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
6061 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
6121 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
6181 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
6241 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
6301 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
6361 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
6421 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
6481 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
6541 agatggttgt tcaagacgat ctacgaacgc agtggcggcg ccggagagtt caagaagttc
6601 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
6661 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
6721 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
6781 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
6841 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
6901 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
6961 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
7021 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
7081 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
7141 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
7201 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
7261 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
7321 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
7381 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
7441 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
7501 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
7561 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
7621 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
7681 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
7741 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
7801 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
7861 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
7921 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
7981 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
8041 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
8101 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
8161 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
8221 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
8281 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gaacaataaa
8341 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
8401 gtcttgctct aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
8461 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga
8521 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
8581 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
8641 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca
8701 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
8761 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
8821 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
8881 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
8941 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
9001 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
9061 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
9121 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
9181 tgagtttttc taatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
9241 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
9301 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
9361 gatgggctgc ctgtatcgag tg
//
