pAcGP67A

价格:2500元

联系方式:I47-825O-882O

买家导航

pAcGP67-A载体基本信息

出品公司: BD Bioscience
载体名称: pAcGP67-A , pAcGP67A
质粒类型: 昆虫杆状病毒表达细胞,杆状病毒转移载体
启动子: Polyhedrin
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 9761 bp
5' 测序引物:   polyhedrin Forward: AAATGATAACCATCTCGC
3' 测序引物: polyhedrin Reverse: GTCCAAGTTTCCCTG
载体标签: N-GP67 signal
载体抗性: 氨苄
筛选标记: --
备注:
载体含有GP67分泌信号肽。
产品货号: 554756
稳定性: 稳表达
组成型: 非组成型
病毒/非病毒: 杆状病毒

pAcGP67-A载体质粒图谱和多克隆位点信息

pAcGP67A载体图谱



pAcGP67A多克隆位点

pAcGP67-A载体简介

酸性糖蛋白gp67(又名gp64)是AcNPV杆状病毒上最丰富的信封表面糖蛋白,是杆状病毒粒子进入易感染的昆虫细胞的必需蛋白。由于大量的该蛋白被细胞分泌出来,并且铆钉在病毒膜粒子上。该病毒基因包含有一个最有效的杆状病毒编码的蛋白分泌信号肽序列。所以,我们制备了这个杆状病毒转移载体(pAcGP67-A, B, C),该载体含有gp67蛋白分泌信号肽。在载体上,该信号肽位于多克隆位点上游。目的基因可以插入到多克隆位点中,同时利用gp67蛋白分泌信号肽在PH启动子作用下进行蛋白表达。这使得能够将非分泌蛋白通过分泌的方式进行蛋白表达,并且纯化出来。

pAcGP67-A载体序列

LOCUS       pAcGP67A	9761 bp 	DNA	SYN
DEFINITION  pAcGP67A
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="pAcGP67A"
                     /mol_type="other DNA"
     CDS             76..582
                     /label="ORF frame 1"
     CDS             complement(614..1600)
                     /label="ORF frame 2"
     CDS             complement(1868..2323)
                     /label="ORF frame 2"
     CDS             2662..3294
                     /label="ORF frame 1"
     misc_feature    2844..3990
                     /label="Ac_5_flank"
     CDS             complement(3332..3937)
                     /label="ORF frame 2"
     promoter        3991..4091
                     /label="PH_promoter"
     misc_feature    4017..4034
                     /label="polyhedrin_fwd_primer"
     CDS             4135..4863
                     /label="ORF frame 1"
     misc_feature    complement(4410..4424)
                     /label="polyhedrinn_rev_primer"
     misc_feature    4762..4863
                     /label="3_AcPH"
     misc_feature    complement(4779..4796)
                     /label="Baculovirs_rev_primer"
     misc_feature    4877..6300
                     /label="Ac_3_flank"
     CDS             complement(4893..6518)
                     /label="ORF frame 1"
     misc_feature    complement(4975..5773)
                     /label="r_ORF1629"
     CDS             6517..7107
                     /label="ORF frame 1"
     promoter        complement(7138..7156)
                     /label="M13_reverse_primer"
     misc_feature    complement(7155..7177)
                     /label="M13_pUC_rev_primer"
     promoter        complement(7191..7220)
                     /label="lac_promoter"
     gene            complement(8303..9163)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(8303..9163)
                     /label="ORF frame 2"
     promoter        complement(9205..9233)
                     /label="AmpR_promoter"
     misc_feature    complement(9392..9414)
                     /label="pGEX_3_primer"
     misc_feature    complement(9613..9756)
                     /label="lacZ_a"
     misc_feature    9727..9749
                     /label="M13_pUC_fwd_primer"
     promoter        9742..9758
                     /label="M13_forward20_primer"
ORIGIN
    1 AAGCTTTACT CGTAAAGCGA GTTGAAGGAT CATATTTAGT TGCGTTTATG AGATAAGATT
   61 GAAAGCACGT GTAAAATGTT TCCCGCGCGT TGGCACAACT ATTTACAATG CGGCCAAGTT
  121 ATAAAAGATT CTAATCTGAT ATGTTTTAAA ACACCTTTGC GGCCCGAGTT GTTTGCGTAC
  181 GTGACTAGCG AAGAAGATGT GTGGACCGCA GAACAGATAG TAAAACAAAA CCCTAGTATT
  241 GGAGCAATAA TCGATTTAAC CAACACGTCT AAATATTATG ATGGTGTGCA TTTTTTGCGG
  301 GCGGGCCTGT TATACAAAAA AATTCAAGTA CCTGGCCAGA CTTTGCCGCC TGAAAGCATA
  361 GTTCAAGAAT TTATTGACAC GGTAAAAGAA TTTACAGAAA AGTGTCCCGG CATGTTGGTG
  421 GGCGTGCACT GCACACACGG TATTAATCGC ACCGGTTACA TGGTGTGCAG ATATTTAATG
  481 CACACCCTGG GTATTGCGCC GCAGGAAGCC ATAGATAGAT TCGAAAAAGC CAGAGGTCAC
  541 AAAATTGAAA GACAAAATTA CGTTCAAGAT TTATTAATTT AATTAATATT ATTTGCATTC
  601 TTTAACAAAT ACTTTATCCT ATTTTCAAAT TGTTGCGCTT CTTCCAGCGA ACCAAAACTA
  661 TGCTTCGCTT GCTCCGTTTA GCTTGTAGCC GATCAGTGGC GTTGTTCCAA TCGACGGTAG
  721 GATTAGGCCG GATATTCTCC ACCACAATGT TGGCAACGTT GATGTTACGT TTATGCTTTT
  781 GGTTTTCCAC GTACGTCTTT TGGCCGGTAA TAGCCGTAAA CGTAGTGCCG TCGCGCGTCA
  841 CGCACAACAC CGGATGTTTG CGCTTGTCCG CGGGGTATTG AACCGCGCGA TCCGACAAAT
  901 CCACCACTTT GGCAACTAAA TCGGTGACCT GCGCGTCTTT TTTCTGCATT ATTTCGTCTT
  961 TCTTTTGCAT GGTTTCCTGG AAGCCGGTGT ACATGCGGTT TAGATCAGTC ATGACGCGCG
 1021 TGACCTGCAA ATCTTTGGCC TCGATCTGCT TGTCCTTGAT GGCAACGATG CGTTCAATAA
 1081 ACTCTTGTTT TTTAACAAGT TCCTCGGTTT TTTGCGCCAC CACCGCTTGC AGCGCGTTTG
 1141 TGTGCTCGGT GAATGTCGCA ATCAGCTTAG TCACCAACTG TTTGCTCTCC TCCTCCCGTT
 1201 GTTTGATCGC GGGATCGTAC TTGCCGGTGC AGAGCACTTG AGGAATTACT TCTTCTAAAA
 1261 GCCATTCTTG TAATTCTATG GCGTAAGGCA ATTTGGACTT CATAATCAGC TGAATCACGC
 1321 CGGATTTAGT AATGAGCACT GTATGCGGCT GCAAATACAG CGGGTCGCCC CTTTTCACGA
 1381 CGCTGTTAGA GGTAGGGCCC CCATTTTGGA TGGTCTGCTC AAATAACGAT TTGTATTTAT
 1441 TGTCTACATG AACACGTATA GCTTTATCAC AAACTGTATA TTTTAAACTG TTAGCGACGT
 1501 CCTTGGCCAC GAACCGGACC TGTTGGTCGC GCTCTAGCAC GTACCGCAGG TTGAACGTAT
 1561 CTTCTCCAAA TTTAAATTCT CCAATTTTAA CGCGAGCCAT TTTGATACAC GTGTGTCGAT
 1621 TTTGCAACAA CTATTGTTTT TTAACGCAAA CTAAACTTAT TGTGGTAAGC AATAATTAAA
 1681 TATGGGGGAA CATGCGCCGC TACAACACTC GTCGTTATGA ACGCAGACGG CGCCGGTCTC
 1741 GGCGCAAGCG GCTAAAACGT GTTGCGCGTT CAACGCGGCA AACATCGCAA AAGCCAATAG
 1801 TACAGTTTTG ATTTGCATAT TAACGGCGAT TTTTTAAATT ATCTTATTTA ATAAATAGTT
 1861 ATGACGCCTA CAACTCCCCG CCCGCGTTGA CTCGCTGCAC CTCGAGCAGT TCGTTGACGC
 1921 CTTCCTCCGT GTGGCCGAAC ACGTCGAGCG GGTGGTCGAT GACCAGCGGC GTGCCGCACG
 1981 CGACGCACAA GTATCTGTAC ACCGAATGAT CGTCGGGCGA AGGCACGTCG GCCTCCAAGT
 2041 GGCAATATTG GCAAATTCGA AAATATATAC AGTTGGGTTG TTTGCGCATA TCTATCGTGG
 2101 CGTTGGGCAT GTACGTCCGA ACGTTGATTT GCATGCAAGC CGAAATTAAA TCATTGCGAT
 2161 TAGTGCGATT AAAACGTTGT ACATCCTCGC TTTTAATCAT GCCGTCGATT AAATCGCGCA
 2221 ATCGAGTCAA GTGATCAAAG TGTGGAATAA TGTTTTCTTT GTATTCCCGA GTCAAGCGCA
 2281 GCGCGTATTT TAACAAACTA GCCATCTTGT AAGTTAGTTT CATTTAATGC AACTTTATCC
 2341 AATAATATAT TATGTATCGC ACGTCAAGAA TTAACAATGC GCCCGTTGTC GCATCTCAAC
 2401 ACGACTATGA TAGAGATCAA ATAAAGCGCG AATTAAATAG CTTGCGACGC AACGTGCACG
 2461 ATCTGTGCAC GCGTTCCGGC ACGAGCTTTG ATTGTAATAA GTTTTTACGA AGCGATGACA
 2521 TGACCCCCGT AGTGACAACG ATCACGCCCA AAAGAACTGC CGACTACAAA ATTACCGAGT
 2581 ATGTCGGTGA CGTTAAAACT ATTAAGCCAT CCAATCGACC GTTAGTCGAA TCAGGACCGC
 2641 TGGTGCGAGA AGCCGCGAAG TATGGCGAAT GCATCGTATA ACGTGTGGAG TCCGCTCATT
 2701 AGAGCGTCAT GTTTAGACAA GAAAGCTACA TATTTAATTG ATCCCGATGA TTTTATTGAT
 2761 AAATTGACCC TAACTCCATA CACGGTATTC TACAATGGCG GGGTTTTGGT CAAAATTTCC
 2821 GGACTGCGAT TGTACATGCT GTTAACGGCT CCGCCCACTA TTAATGAAAT TAAAAATTCC
 2881 AATTTTAAAA AACGCAGCAA GAGAAACATT TGTATGAAAG AATGCGTAGA AGGAAAGAAA
 2941 AATGTCGTCG ACATGCTGAA CAACAAGATT AATATGCCTC CGTGTATAAA AAAAATATTG
 3001 AACGATTTGA AAGAAAACAA TGTACCGCGC GGCGGTATGT ACAGGAAGAG GTTTATACTA
 3061 AACTGTTACA TTGCAAACGT GGTTTCGTGT GCCAAGTGTG AAAACCGATG TTTAATCAAG
 3121 GCTCTGACGC ATTTCTACAA CCACGACTCC AAGTGTGTGG GTGAAGTCAT GCATCTTTTA
 3181 ATCAAATCCC AAGATGTGTA TAAACCACCA AACTGCCAAA AAATGAAAAC TGTCGACAAG
 3241 CTCTGTCCGT TTGCTGGCAA CTGCAAGGGT CTCAATCCTA TTTGTAATTA TTGAATAATA
 3301 AAACAATTAT AAATGCTAAA TTTGTTTTTT ATTAACGATA CAAACCAAAC GCAACAAGAA
 3361 CATTTGTAGT ATTATCTATA ATTGAAAACG CGTAGTTATA ATCGCTGAGG TAATATTTAA
 3421 AATCATTTTC AAATGATTCA CAGTTAATTT GCGACAATAT AATTTTATTT TCACATAAAC
 3481 TAGACGCCTT GTCGTCTTCT TCTTCGTATT CCTTCTCTTT TTCATTTTTC TCCTCATAAA
 3541 AATTAACATA GTTATTATCG TATCCATATA TGTATCTATC GTATAGAGTA AATTTTTTGT
 3601 TGTCATAAAT ATATATGTCT TTTTTAATGG GGTGTATAGT ACCGCTGCGC ATAGTTTTTC
 3661 TGTAATTTAC AACAGTGCTA TTTTCTGGTA GTTCTTCGGA GTGTGTTGCT TTAATTATTA
 3721 AATTTATATA ATCAATGAAT TTGGGATCGT CGGTTTTGTA CAATATGTTG CCGGCATAGT
 3781 ACGCAGCTTC TTCTAGTTCA ATTACACCAT TTTTTAGCAG CACCGGATTA ACATAACTTT
 3841 CCAAAATGTT GTACGAACCG TTAAACAAAA ACAGTTCACC TCCCTTTTCT ATACTATTGT
 3901 CTGCGAGCAG TTGTTTGTTG TTAAAAATAA CAGCCATTGT AATGAGACGC ACAAACTAAT
 3961 ATCACAAACT GGAAATGTCT ATCAATATAT AGTTGCTGAT ATCATGGAGA TAATTAAAAT
 4021 GATAACCATC TCGCAAATAA ATAAGTATTT TACTGTTTTC GTAACAGTTT TGTAATAAAA
 4081 AAACCTATAA ATATtCCGGA TTATTCATAC CGTCCCACCA TCGGGCGcGG ATCTATGCTA
 4141 CTAGTAAATC AGTCACACCA AGGCTTCAAT AAGGAACACA CAAGCAAGAT GGTAAGCGCT
 4201 ATTGTTTTAT ATGTGCTTTT GGCGGCGGCG GCGCATTCTG CCTTTGCGGC GGATCCCGGG
 4261 TACCTTCTAG AATTCCGGAG CGGCCGCTGC AGATCTgatc cTTTCCTGGG ACCCGGCAAG
 4321 AACCAAAAAC TCACTCTCTT CAAGGAAATC CGTAATGTTA AACCCGACAC GATGAAGCTT
 4381 GTCGTTGGAT GGAAAGGAAA AGAGTTCTAC AGGGAAACTT GGACCCGCTT CATGGAAGAC
 4441 AGCTTCCCCA TTGTTAACGA CCAAGAAGTG ATGGATGTTT TCCTTGTTGT CAACATGCGT
 4501 CCCACTAGAC CCAACCGTTG TTACAAATTC CTGGCCCAAC ACGCTCTGCG TTGCGACCCC
 4561 GACTATGTAC CTCATGACGT GATTAGGATC GTCGAGCCTT CATGGGTGGG CAGCAACAAC
 4621 GAGTACCGCA TCAGCCTGGC TAAGAAGGGC GGCGGCTGCC CAATAATGAA CCTTCACTCT
 4681 GAGTACACCA ACTCGTTCGA ACAGTTCATC GATCGTGTCA TCTGGGAGAA CTTCTACAAG
 4741 CCCATCGTTT ACATCGGTAC CGACTCTGCT GAAGAGGAGG AAATTCTCCT TGAAGTTTCC
 4801 CTGGTGTTCA AAGTAAAGGA GTTTGCACCA GACGCACCTC TGTTCACTGG TCCGGCGTAT
 4861 TAAAACACGA TACATTGTTA TTAGTACATT TATTAAGCGC TAGATTCTGT GCGTTGTTGA
 4921 TTTACAGACA ATTGTTGTAC GTATTTTAAT AATTCATTAA ATTTATAATC TTTAGGGTGG
 4981 TATGTTAGAG CGAAAATCAA ATGATTTTCA GCGTCTTTAT ATCTGAATTT AAATATTAAA
 5041 TCCTCAATAG ATTTGTAAAA TAGGTTTCGA TTAGTTTCAA ACAAGGGTTG TTTTTCCGAA
 5101 CCGATGGCTG GACTATCTAA TGGATTTTCG CTCAACGCCA CAAAACTTGC CAAATCTTGT
 5161 AGCAGCAATC TAGCTTTGTC GATATTCGTT TGTGTTTTGT TTTGTAATAA AGGTTCGACG
 5221 TCGTTCAAAA TATTATGCGC TTTTGTATTT CTTTCATCAC TGTCGTTAGT GTACAATTGA
 5281 CTCGACGTAA ACACGTTAAA TAAAGCTTGG ACATATTTAA CATCGGGCGT GTTAGCTTTA
 5341 TTAGGCCGAT TATCGTCGTC GTCCCAACCC TCGTCGTTAG AAGTTGCTTC CGAAGACGAT
 5401 TTTGCCATAG CCACACGACG CCTATTAATT GTGTCGGCTA ACACGTCCGC GATCAAATTT
 5461 GTAGTTGAGC TTTTTGGAAT TATTTCTGAT TGCGGGCGTT TTTGGGCGGG TTTCAATCTA
 5521 ACTGTGCCCG ATTTTAATTC AGACAACACG TTAGAAAGCG ATGGTGCAGG CGGTGGTAAC
 5581 ATTTCAGACG GCAAATCTAC TAATGGCGGC GGTGGTGGAG CTGATGATAA ATCTACCATC
 5641 GGTGGAGGCG CAGGCGGGGC TGGCGGCGGA GGCGGAGGCG GAGGTGGTGG CGGTGATGCA
 5701 GACGGCGGTT TAGGCTCAAA TGTCTCTTTA GGCAACACAG TCGGCACCTC AACTATTGTA
 5761 CTGGTTTCGG GCGCCGTTTT TGGTTTGACC GGTCTGAGAC GAGTGCGATT TTTTTCGTTT
 5821 CTAATAGCTT CCAACAATTG TTGTCTGTCG TCTAAAGGTG CAGCGGGTTG AGGTTCCGTC
 5881 GGCATTGGTG GAGCGGGCGG CAATTCAGAC ATCGATGGTG GTGGTGGTGG TGGAGGCGCT
 5941 GGAATGTTAG GCACGGGAGA AGGTGGTGGC GGCGGTGCCG CCGGTATAAT TTGTTCTGGT
 6001 TTAGTTTGTT CGCGCACGAT TGTGGGCACC GGCGCAGGCG CCGCTGGCTG CACAACGGAA
 6061 GGTCGTCTGC TTCGAGGCAG CGCTTGGGGT GGTGGCAATT CAATATTATA ATTGGAATAC
 6121 AAATCGTAAA AATCTGCTAT AAGCATTGTA ATTTCGCTAT CGTTTACCGT GCCGATATTT
 6181 AACAACCGCT CAATGTAAGC AATTGTATTG TAAAGAGATT GTCTCAAGCT CCGCACGCCG
 6241 ATAACAAGCC TTTTCATTTT TACTACAGCA TTGTAGTGGC GAGACACTTC GCTGTCGTCG
 6301 ACGTACATGT ATGCTTTGTT GTCAAAAACG TCGTTGGCAA GCTTTAAAAT ATTTAAAAGA
 6361 ACATCTCTGT TCAGCACCAC TGTGTTGTCG TAAATGTTGT TTTTGATAAT TTGCGCTTCC
 6421 GCAGTATCGA CACGTTCAAA AAATTGATGC GCATCAATTT TGTTGTTCCT ATTATTGAAT
 6481 AAATAAGATT GTACAGATTC ATATCTACGA TTCGTCATGG CCACCACAAA TGCTACGCTG
 6541 CAAACGCTGG TACAATTTTA CGAAAACTGC AAAAACGTCA AAACTCGGTA TAAAATAATC
 6601 AACGGGCGCT TTGGCAAAAT ATCTATTTTA TCGCACAAGC CCACTAGCAA ATTGTATTTG
 6661 CAGAAAACAA TTTCGGCGCA CAATTTTAAC GCTGACGAAA TAAAAGTTCA CCAGTTAATG
 6721 AGCGACCACC CAAATTTTAT AAAAATCTAT TTTAATCACG GTTCCATCAA CAACCAAGTG
 6781 ATCGTGATGG ACTACATTGA CTGTCCCGAT TTATTTGAAA CACTACAAAT TAAAGGCGAG
 6841 CTTTCGTACC AACTTGTTAG CAATATTATT AGACAGCTGT GTGAAGCGCT CAACGATTTG
 6901 CACAAGCACA ATTTCATACA CAACGACATA AAACTCGAAA ATGTCTTATA TTTCGAAGCA
 6961 CTTGATCGCG TGTATGTTTG CGATTACGGA TTGTGCAAAC ACGAAAACTC ACTTAGCGTG
 7021 CACGACGGCA CGTTGGAGTA TTTTAGTCCG GAAAAAATTC GACACACAAC TATGCACGTT
 7081 TCGTTTGACT GGTACGCGGC GTGTTAACAT ACAAGTTGCT AACCGGcggt tcGTAATCAT
 7141 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG
 7201 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG
 7261 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA
 7321 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA
 7381 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG
 7441 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC
 7501 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC
 7561 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC
 7621 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC
 7681 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA
 7741 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC
 7801 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA
 7861 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG
 7921 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA
 7981 GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG
 8041 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC
 8101 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT
 8161 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA
 8221 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT
 8281 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA
 8341 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC
 8401 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG
 8461 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG
 8521 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT
 8581 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT
 8641 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT
 8701 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA
 8761 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA
 8821 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT
 8881 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC
 8941 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA
 9001 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT
 9061 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG
 9121 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT
 9181 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT
 9241 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT
 9301 AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC
 9361 GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG
 9421 TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG
 9481 GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG
 9541 TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC
 9601 GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC
 9661 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG
 9721 GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGC C
//

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