| 出品公司: | BD Bioscience |
|---|---|
| 载体名称: | pAcGP67-A , pAcGP67A |
| 质粒类型: | 昆虫杆状病毒表达细胞，杆状病毒转移载体 |
| 启动子: | Polyhedrin |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 9761 bp |
| 5' 测序引物: | polyhedrin Forward: AAATGATAACCATCTCGC |
| 3' 测序引物: | polyhedrin Reverse: GTCCAAGTTTCCCTG |
| 载体标签: | N-GP67 signal |
| 载体抗性: | 氨苄 |
| 筛选标记: | -- |
| 备注: |
载体含有GP67分泌信号肽。
|
| 产品货号: | 554756 |
| 稳定性: | 稳表达 |
| 组成型: | 非组成型 |
| 病毒/非病毒: | 杆状病毒 |
买家导航
pAcGP67-A载体基本信息
pAcGP67-A载体图谱质粒图谱和多克隆位点信息
pAcGP67-A载体简介
酸性糖蛋白gp67（又名gp64）是AcNPV杆状病毒上最丰富的信封表面糖蛋白，是杆状病毒粒子进入易感染的昆虫细胞的必需蛋白。由于大量的该蛋白被细胞分泌出来，并且铆钉在病毒膜粒子上。该病毒基因包含有一个最有效的杆状病毒编码的蛋白分泌信号肽序列。所以，我们制备了这个杆状病毒转移载体(pAcGP67-A, B, C),该载体含有gp67蛋白分泌信号肽。在载体上，该信号肽位于多克隆位点上游。目的基因可以插入到多克隆位点中，同时利用gp67蛋白分泌信号肽在PH启动子作用下进行蛋白表达。这使得能够将非分泌蛋白通过分泌的方式进行蛋白表达，并且纯化出来。
pAcGP67-A载体序列
LOCUS pAcGP67A 9761 bp DNA SYN
DEFINITION pAcGP67A
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..9761
/organism="pAcGP67A"
/mol_type="other DNA"
CDS 76..582
/label="ORF frame 1"
CDS complement(614..1600)
/label="ORF frame 2"
CDS complement(1868..2323)
/label="ORF frame 2"
CDS 2662..3294
/label="ORF frame 1"
misc_feature 2844..3990
/label="Ac_5_flank"
CDS complement(3332..3937)
/label="ORF frame 2"
promoter 3991..4091
/label="PH_promoter"
misc_feature 4017..4034
/label="polyhedrin_fwd_primer"
CDS 4135..4863
/label="ORF frame 1"
misc_feature complement(4410..4424)
/label="polyhedrinn_rev_primer"
misc_feature 4762..4863
/label="3_AcPH"
misc_feature complement(4779..4796)
/label="Baculovirs_rev_primer"
misc_feature 4877..6300
/label="Ac_3_flank"
CDS complement(4893..6518)
/label="ORF frame 1"
misc_feature complement(4975..5773)
/label="r_ORF1629"
CDS 6517..7107
/label="ORF frame 1"
promoter complement(7138..7156)
/label="M13_reverse_primer"
misc_feature complement(7155..7177)
/label="M13_pUC_rev_primer"
promoter complement(7191..7220)
/label="lac_promoter"
gene complement(8303..9163)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(8303..9163)
/label="ORF frame 2"
promoter complement(9205..9233)
/label="AmpR_promoter"
misc_feature complement(9392..9414)
/label="pGEX_3_primer"
misc_feature complement(9613..9756)
/label="lacZ_a"
misc_feature 9727..9749
/label="M13_pUC_fwd_primer"
promoter 9742..9758
/label="M13_forward20_primer"
ORIGIN
1 AAGCTTTACT CGTAAAGCGA GTTGAAGGAT CATATTTAGT TGCGTTTATG AGATAAGATT
61 GAAAGCACGT GTAAAATGTT TCCCGCGCGT TGGCACAACT ATTTACAATG CGGCCAAGTT
121 ATAAAAGATT CTAATCTGAT ATGTTTTAAA ACACCTTTGC GGCCCGAGTT GTTTGCGTAC
181 GTGACTAGCG AAGAAGATGT GTGGACCGCA GAACAGATAG TAAAACAAAA CCCTAGTATT
241 GGAGCAATAA TCGATTTAAC CAACACGTCT AAATATTATG ATGGTGTGCA TTTTTTGCGG
301 GCGGGCCTGT TATACAAAAA AATTCAAGTA CCTGGCCAGA CTTTGCCGCC TGAAAGCATA
361 GTTCAAGAAT TTATTGACAC GGTAAAAGAA TTTACAGAAA AGTGTCCCGG CATGTTGGTG
421 GGCGTGCACT GCACACACGG TATTAATCGC ACCGGTTACA TGGTGTGCAG ATATTTAATG
481 CACACCCTGG GTATTGCGCC GCAGGAAGCC ATAGATAGAT TCGAAAAAGC CAGAGGTCAC
541 AAAATTGAAA GACAAAATTA CGTTCAAGAT TTATTAATTT AATTAATATT ATTTGCATTC
601 TTTAACAAAT ACTTTATCCT ATTTTCAAAT TGTTGCGCTT CTTCCAGCGA ACCAAAACTA
661 TGCTTCGCTT GCTCCGTTTA GCTTGTAGCC GATCAGTGGC GTTGTTCCAA TCGACGGTAG
721 GATTAGGCCG GATATTCTCC ACCACAATGT TGGCAACGTT GATGTTACGT TTATGCTTTT
781 GGTTTTCCAC GTACGTCTTT TGGCCGGTAA TAGCCGTAAA CGTAGTGCCG TCGCGCGTCA
841 CGCACAACAC CGGATGTTTG CGCTTGTCCG CGGGGTATTG AACCGCGCGA TCCGACAAAT
901 CCACCACTTT GGCAACTAAA TCGGTGACCT GCGCGTCTTT TTTCTGCATT ATTTCGTCTT
961 TCTTTTGCAT GGTTTCCTGG AAGCCGGTGT ACATGCGGTT TAGATCAGTC ATGACGCGCG
1021 TGACCTGCAA ATCTTTGGCC TCGATCTGCT TGTCCTTGAT GGCAACGATG CGTTCAATAA
1081 ACTCTTGTTT TTTAACAAGT TCCTCGGTTT TTTGCGCCAC CACCGCTTGC AGCGCGTTTG
1141 TGTGCTCGGT GAATGTCGCA ATCAGCTTAG TCACCAACTG TTTGCTCTCC TCCTCCCGTT
1201 GTTTGATCGC GGGATCGTAC TTGCCGGTGC AGAGCACTTG AGGAATTACT TCTTCTAAAA
1261 GCCATTCTTG TAATTCTATG GCGTAAGGCA ATTTGGACTT CATAATCAGC TGAATCACGC
1321 CGGATTTAGT AATGAGCACT GTATGCGGCT GCAAATACAG CGGGTCGCCC CTTTTCACGA
1381 CGCTGTTAGA GGTAGGGCCC CCATTTTGGA TGGTCTGCTC AAATAACGAT TTGTATTTAT
1441 TGTCTACATG AACACGTATA GCTTTATCAC AAACTGTATA TTTTAAACTG TTAGCGACGT
1501 CCTTGGCCAC GAACCGGACC TGTTGGTCGC GCTCTAGCAC GTACCGCAGG TTGAACGTAT
1561 CTTCTCCAAA TTTAAATTCT CCAATTTTAA CGCGAGCCAT TTTGATACAC GTGTGTCGAT
1621 TTTGCAACAA CTATTGTTTT TTAACGCAAA CTAAACTTAT TGTGGTAAGC AATAATTAAA
1681 TATGGGGGAA CATGCGCCGC TACAACACTC GTCGTTATGA ACGCAGACGG CGCCGGTCTC
1741 GGCGCAAGCG GCTAAAACGT GTTGCGCGTT CAACGCGGCA AACATCGCAA AAGCCAATAG
1801 TACAGTTTTG ATTTGCATAT TAACGGCGAT TTTTTAAATT ATCTTATTTA ATAAATAGTT
1861 ATGACGCCTA CAACTCCCCG CCCGCGTTGA CTCGCTGCAC CTCGAGCAGT TCGTTGACGC
1921 CTTCCTCCGT GTGGCCGAAC ACGTCGAGCG GGTGGTCGAT GACCAGCGGC GTGCCGCACG
1981 CGACGCACAA GTATCTGTAC ACCGAATGAT CGTCGGGCGA AGGCACGTCG GCCTCCAAGT
2041 GGCAATATTG GCAAATTCGA AAATATATAC AGTTGGGTTG TTTGCGCATA TCTATCGTGG
2101 CGTTGGGCAT GTACGTCCGA ACGTTGATTT GCATGCAAGC CGAAATTAAA TCATTGCGAT
2161 TAGTGCGATT AAAACGTTGT ACATCCTCGC TTTTAATCAT GCCGTCGATT AAATCGCGCA
2221 ATCGAGTCAA GTGATCAAAG TGTGGAATAA TGTTTTCTTT GTATTCCCGA GTCAAGCGCA
2281 GCGCGTATTT TAACAAACTA GCCATCTTGT AAGTTAGTTT CATTTAATGC AACTTTATCC
2341 AATAATATAT TATGTATCGC ACGTCAAGAA TTAACAATGC GCCCGTTGTC GCATCTCAAC
2401 ACGACTATGA TAGAGATCAA ATAAAGCGCG AATTAAATAG CTTGCGACGC AACGTGCACG
2461 ATCTGTGCAC GCGTTCCGGC ACGAGCTTTG ATTGTAATAA GTTTTTACGA AGCGATGACA
2521 TGACCCCCGT AGTGACAACG ATCACGCCCA AAAGAACTGC CGACTACAAA ATTACCGAGT
2581 ATGTCGGTGA CGTTAAAACT ATTAAGCCAT CCAATCGACC GTTAGTCGAA TCAGGACCGC
2641 TGGTGCGAGA AGCCGCGAAG TATGGCGAAT GCATCGTATA ACGTGTGGAG TCCGCTCATT
2701 AGAGCGTCAT GTTTAGACAA GAAAGCTACA TATTTAATTG ATCCCGATGA TTTTATTGAT
2761 AAATTGACCC TAACTCCATA CACGGTATTC TACAATGGCG GGGTTTTGGT CAAAATTTCC
2821 GGACTGCGAT TGTACATGCT GTTAACGGCT CCGCCCACTA TTAATGAAAT TAAAAATTCC
2881 AATTTTAAAA AACGCAGCAA GAGAAACATT TGTATGAAAG AATGCGTAGA AGGAAAGAAA
2941 AATGTCGTCG ACATGCTGAA CAACAAGATT AATATGCCTC CGTGTATAAA AAAAATATTG
3001 AACGATTTGA AAGAAAACAA TGTACCGCGC GGCGGTATGT ACAGGAAGAG GTTTATACTA
3061 AACTGTTACA TTGCAAACGT GGTTTCGTGT GCCAAGTGTG AAAACCGATG TTTAATCAAG
3121 GCTCTGACGC ATTTCTACAA CCACGACTCC AAGTGTGTGG GTGAAGTCAT GCATCTTTTA
3181 ATCAAATCCC AAGATGTGTA TAAACCACCA AACTGCCAAA AAATGAAAAC TGTCGACAAG
3241 CTCTGTCCGT TTGCTGGCAA CTGCAAGGGT CTCAATCCTA TTTGTAATTA TTGAATAATA
3301 AAACAATTAT AAATGCTAAA TTTGTTTTTT ATTAACGATA CAAACCAAAC GCAACAAGAA
3361 CATTTGTAGT ATTATCTATA ATTGAAAACG CGTAGTTATA ATCGCTGAGG TAATATTTAA
3421 AATCATTTTC AAATGATTCA CAGTTAATTT GCGACAATAT AATTTTATTT TCACATAAAC
3481 TAGACGCCTT GTCGTCTTCT TCTTCGTATT CCTTCTCTTT TTCATTTTTC TCCTCATAAA
3541 AATTAACATA GTTATTATCG TATCCATATA TGTATCTATC GTATAGAGTA AATTTTTTGT
3601 TGTCATAAAT ATATATGTCT TTTTTAATGG GGTGTATAGT ACCGCTGCGC ATAGTTTTTC
3661 TGTAATTTAC AACAGTGCTA TTTTCTGGTA GTTCTTCGGA GTGTGTTGCT TTAATTATTA
3721 AATTTATATA ATCAATGAAT TTGGGATCGT CGGTTTTGTA CAATATGTTG CCGGCATAGT
3781 ACGCAGCTTC TTCTAGTTCA ATTACACCAT TTTTTAGCAG CACCGGATTA ACATAACTTT
3841 CCAAAATGTT GTACGAACCG TTAAACAAAA ACAGTTCACC TCCCTTTTCT ATACTATTGT
3901 CTGCGAGCAG TTGTTTGTTG TTAAAAATAA CAGCCATTGT AATGAGACGC ACAAACTAAT
3961 ATCACAAACT GGAAATGTCT ATCAATATAT AGTTGCTGAT ATCATGGAGA TAATTAAAAT
4021 GATAACCATC TCGCAAATAA ATAAGTATTT TACTGTTTTC GTAACAGTTT TGTAATAAAA
4081 AAACCTATAA ATATtCCGGA TTATTCATAC CGTCCCACCA TCGGGCGcGG ATCTATGCTA
4141 CTAGTAAATC AGTCACACCA AGGCTTCAAT AAGGAACACA CAAGCAAGAT GGTAAGCGCT
4201 ATTGTTTTAT ATGTGCTTTT GGCGGCGGCG GCGCATTCTG CCTTTGCGGC GGATCCCGGG
4261 TACCTTCTAG AATTCCGGAG CGGCCGCTGC AGATCTgatc cTTTCCTGGG ACCCGGCAAG
4321 AACCAAAAAC TCACTCTCTT CAAGGAAATC CGTAATGTTA AACCCGACAC GATGAAGCTT
4381 GTCGTTGGAT GGAAAGGAAA AGAGTTCTAC AGGGAAACTT GGACCCGCTT CATGGAAGAC
4441 AGCTTCCCCA TTGTTAACGA CCAAGAAGTG ATGGATGTTT TCCTTGTTGT CAACATGCGT
4501 CCCACTAGAC CCAACCGTTG TTACAAATTC CTGGCCCAAC ACGCTCTGCG TTGCGACCCC
4561 GACTATGTAC CTCATGACGT GATTAGGATC GTCGAGCCTT CATGGGTGGG CAGCAACAAC
4621 GAGTACCGCA TCAGCCTGGC TAAGAAGGGC GGCGGCTGCC CAATAATGAA CCTTCACTCT
4681 GAGTACACCA ACTCGTTCGA ACAGTTCATC GATCGTGTCA TCTGGGAGAA CTTCTACAAG
4741 CCCATCGTTT ACATCGGTAC CGACTCTGCT GAAGAGGAGG AAATTCTCCT TGAAGTTTCC
4801 CTGGTGTTCA AAGTAAAGGA GTTTGCACCA GACGCACCTC TGTTCACTGG TCCGGCGTAT
4861 TAAAACACGA TACATTGTTA TTAGTACATT TATTAAGCGC TAGATTCTGT GCGTTGTTGA
4921 TTTACAGACA ATTGTTGTAC GTATTTTAAT AATTCATTAA ATTTATAATC TTTAGGGTGG
4981 TATGTTAGAG CGAAAATCAA ATGATTTTCA GCGTCTTTAT ATCTGAATTT AAATATTAAA
5041 TCCTCAATAG ATTTGTAAAA TAGGTTTCGA TTAGTTTCAA ACAAGGGTTG TTTTTCCGAA
5101 CCGATGGCTG GACTATCTAA TGGATTTTCG CTCAACGCCA CAAAACTTGC CAAATCTTGT
5161 AGCAGCAATC TAGCTTTGTC GATATTCGTT TGTGTTTTGT TTTGTAATAA AGGTTCGACG
5221 TCGTTCAAAA TATTATGCGC TTTTGTATTT CTTTCATCAC TGTCGTTAGT GTACAATTGA
5281 CTCGACGTAA ACACGTTAAA TAAAGCTTGG ACATATTTAA CATCGGGCGT GTTAGCTTTA
5341 TTAGGCCGAT TATCGTCGTC GTCCCAACCC TCGTCGTTAG AAGTTGCTTC CGAAGACGAT
5401 TTTGCCATAG CCACACGACG CCTATTAATT GTGTCGGCTA ACACGTCCGC GATCAAATTT
5461 GTAGTTGAGC TTTTTGGAAT TATTTCTGAT TGCGGGCGTT TTTGGGCGGG TTTCAATCTA
5521 ACTGTGCCCG ATTTTAATTC AGACAACACG TTAGAAAGCG ATGGTGCAGG CGGTGGTAAC
5581 ATTTCAGACG GCAAATCTAC TAATGGCGGC GGTGGTGGAG CTGATGATAA ATCTACCATC
5641 GGTGGAGGCG CAGGCGGGGC TGGCGGCGGA GGCGGAGGCG GAGGTGGTGG CGGTGATGCA
5701 GACGGCGGTT TAGGCTCAAA TGTCTCTTTA GGCAACACAG TCGGCACCTC AACTATTGTA
5761 CTGGTTTCGG GCGCCGTTTT TGGTTTGACC GGTCTGAGAC GAGTGCGATT TTTTTCGTTT
5821 CTAATAGCTT CCAACAATTG TTGTCTGTCG TCTAAAGGTG CAGCGGGTTG AGGTTCCGTC
5881 GGCATTGGTG GAGCGGGCGG CAATTCAGAC ATCGATGGTG GTGGTGGTGG TGGAGGCGCT
5941 GGAATGTTAG GCACGGGAGA AGGTGGTGGC GGCGGTGCCG CCGGTATAAT TTGTTCTGGT
6001 TTAGTTTGTT CGCGCACGAT TGTGGGCACC GGCGCAGGCG CCGCTGGCTG CACAACGGAA
6061 GGTCGTCTGC TTCGAGGCAG CGCTTGGGGT GGTGGCAATT CAATATTATA ATTGGAATAC
6121 AAATCGTAAA AATCTGCTAT AAGCATTGTA ATTTCGCTAT CGTTTACCGT GCCGATATTT
6181 AACAACCGCT CAATGTAAGC AATTGTATTG TAAAGAGATT GTCTCAAGCT CCGCACGCCG
6241 ATAACAAGCC TTTTCATTTT TACTACAGCA TTGTAGTGGC GAGACACTTC GCTGTCGTCG
6301 ACGTACATGT ATGCTTTGTT GTCAAAAACG TCGTTGGCAA GCTTTAAAAT ATTTAAAAGA
6361 ACATCTCTGT TCAGCACCAC TGTGTTGTCG TAAATGTTGT TTTTGATAAT TTGCGCTTCC
6421 GCAGTATCGA CACGTTCAAA AAATTGATGC GCATCAATTT TGTTGTTCCT ATTATTGAAT
6481 AAATAAGATT GTACAGATTC ATATCTACGA TTCGTCATGG CCACCACAAA TGCTACGCTG
6541 CAAACGCTGG TACAATTTTA CGAAAACTGC AAAAACGTCA AAACTCGGTA TAAAATAATC
6601 AACGGGCGCT TTGGCAAAAT ATCTATTTTA TCGCACAAGC CCACTAGCAA ATTGTATTTG
6661 CAGAAAACAA TTTCGGCGCA CAATTTTAAC GCTGACGAAA TAAAAGTTCA CCAGTTAATG
6721 AGCGACCACC CAAATTTTAT AAAAATCTAT TTTAATCACG GTTCCATCAA CAACCAAGTG
6781 ATCGTGATGG ACTACATTGA CTGTCCCGAT TTATTTGAAA CACTACAAAT TAAAGGCGAG
6841 CTTTCGTACC AACTTGTTAG CAATATTATT AGACAGCTGT GTGAAGCGCT CAACGATTTG
6901 CACAAGCACA ATTTCATACA CAACGACATA AAACTCGAAA ATGTCTTATA TTTCGAAGCA
6961 CTTGATCGCG TGTATGTTTG CGATTACGGA TTGTGCAAAC ACGAAAACTC ACTTAGCGTG
7021 CACGACGGCA CGTTGGAGTA TTTTAGTCCG GAAAAAATTC GACACACAAC TATGCACGTT
7081 TCGTTTGACT GGTACGCGGC GTGTTAACAT ACAAGTTGCT AACCGGcggt tcGTAATCAT
7141 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG
7201 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG
7261 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA
7321 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA
7381 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG
7441 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC
7501 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC
7561 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC
7621 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC
7681 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA
7741 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC
7801 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA
7861 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG
7921 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA
7981 GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG
8041 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC
8101 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT
8161 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA
8221 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT
8281 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA
8341 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC
8401 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG
8461 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG
8521 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT
8581 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT
8641 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT
8701 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA
8761 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA
8821 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT
8881 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC
8941 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA
9001 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT
9061 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG
9121 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT
9181 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT
9241 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT
9301 AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC
9361 GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG
9421 TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG
9481 GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG
9541 TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC
9601 GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC
9661 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG
9721 GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGC C
//
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pAcGP67-A载体质粒应用举例
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