| 质粒名称: | pAAV-CAG-miRFP2-P2A-GFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000013 |
| 产品用途: | AAV, Expresses miRFP2 and EGFP in cytoplasm of mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | miRFP2, EGFP |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
pAAV-CAG-miRFP2-P2A-GFP
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CAG-miRFP2-P2A-GFP载体质粒基本信息
pAAV-CAG-miRFP2-P2A-GFP质粒图谱载体图谱和pAAV-CAG-miRFP2-P2A-GFP载体序列质粒序列多克隆位点信息
pAAV-CAG-miRFP2-P2A-GFP质粒载体简介
pAAV-CAG-miRFP2-P2A-GFP质粒序列载体序列
LOCUS pAAV-CAG-miRFP2-P2A-GFP 6472 bp DNA circular SYN 27-MAY-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6472)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Jun 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6472
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
regulatory 977..986
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 983..1921
/codon_start=1
/label=miRFP2
/translation="MVAGHASGSPDFGTASPSDCEHEEIHLAGSIQPHGTLLVVSEPDH
RIIQASANAAEFLNLGSVLGIPLAEIDGDLLIKILPHLDLTAEGMPVAVRCRIGNPSTE
YDGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
YDRVMVYRFDERGHGEVYSEIHVTGLESYFGNRYPSSLVPQMARRLYVRQRVRVLVDVT
YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPTHLQFLKNMGVRATLVVSLVVGGKLWGLV
ICHHYLPRFIHFELRAICVLLAEAIATRITAL"
CDS 1946..2002
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 2015..2731
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(2086..2104)
/label=EGFP-R
misc_feature 2756..3344
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(3227..3238)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3387..3508
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(4036..4624)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4795..5655)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5656..5760)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5786..6241)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatggtcgc aggacacgca agtggaagcc ccgactttgg
1021 aaccgccagt ccgagcgact gtgagcacga agaaatccat ctggccggat ccatccagcc
1081 acacggaaca ctgctggtgg tctccgagcc tgaccataga ataattcagg catctgccaa
1141 cgccgctgaa ttcctgaatc tgggaagtgt gctgggaatc cccctggctg agatcgacgg
1201 ggatctactg atcaagattc tgccccacct ggaccttacc gcagaaggaa tgccagtggc
1261 cgtcaggtgc cgcattggaa acccctccac agagtacgac ggactgatgc atcgaccacc
1321 tgaaggagga ctgatcattg agctggaacg ggctgggcca tccattgatc tgagcggaac
1381 actggctccc gcactggagc gaattcgaac tgcaggatcc ctgagggctc tgtgcgacga
1441 tactgtactg ctgttccagc agtgtaccgg ctacgaccga gtgatggtct atcggttcga
1501 tgaacggggg cacggagagg tgtattcaga aattcatgtg actggactgg agagctactt
1561 tggcaacagg tatcctagct ccctcgtgcc acagatggcc cggcggctgt acgtgagaca
1621 gagggtgcgc gtcctggtag atgtcaccta tcagcctgtg ccactggagc caagactgag
1681 tcccctgact ggcagggacc tggatatgtc agggtgtttt ctgcgctcta tgagtcctac
1741 tcacctgcag ttcctgaaga acatgggcgt gcgggcaaca ctggtggtca gcctggtggt
1801 cggaggcaaa ctgtgggggc tggtgatctg ccaccattat ctgccacgct tcatccattt
1861 tgagctgcga gcaatttgtg tgctgctggc cgaagctatc gcaacccgca ttacagccct
1921 gcgaccggtt cacgtgggaa gtggggctac aaacttctca ctgctgaaac aggccgggga
1981 tgtggaggaa aacccaggac cacacgtgac tagtgtgagc aagggcgagg agctgttcac
2041 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
2101 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
2161 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
2221 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
2281 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
2341 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
2401 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
2461 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
2521 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
2581 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
2641 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
2701 cactctcggc atggacgagc tgtacaagta agaattcgat atcaagctta tcgataatca
2761 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
2821 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
2881 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
2941 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
3001 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
3061 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
3121 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
3181 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
3241 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
3301 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
3361 cgggcggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
3421 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
3481 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
3541 ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
3601 aatcgataag gatcttccta gagcatggct acgtagataa gtagcatggc gggttaatca
3661 ttaactacaa ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
3721 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
3781 tgagcgagcg agcgcgcagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
3841 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
3901 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
3961 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
4021 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
4081 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
4141 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
4201 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
4261 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
4321 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
4381 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
4441 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
4501 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
4561 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
4621 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
4681 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
4741 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
4801 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
4861 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
4921 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
4981 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
5041 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
5101 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
5161 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
5221 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
5281 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
5341 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
5401 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
5461 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
5521 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
5581 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
5641 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
5701 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
5761 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
5821 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
5881 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
5941 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
6001 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
6061 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
6121 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
6181 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
6241 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
6301 ctattacgcc agctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt
6361 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
6421 aactccatca ctaggggttc cttgtagtta atgattaacc cgccatgcta ct
//
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