| 质粒名称: | pCMV-Tol2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31823 |
| 存储实验室: | Stephen Ekker |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 3700 bp |
| 完整质粒大小: | 5700 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Tol2 Transposase |
| 物种来源: | Artificial sequence |
| 插入大小: | 2000 bp |
| 启动子 | CMV |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NotI(未破坏) |
| 3' 克隆位点: | NotI(未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | EBV-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-Tol2 was a gift from Stephen Ekker (爱迪基因 plasmid # 31823) |
| 参考文献部分: | Harnessing a high cargo-capacity transposon for genetic applications in vertebrates. Balciunas D, Wangensteen KJ, Wilber A, Bell J, Geurts A, Sivasubbu S, Wang X, Hackett PB, Largaespada DA, McIvor RS, Ekker SC.. PLoS Genet. 2006 Nov 10;2(11):e169. Epub 2006 Aug 28. |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV-Tol2 5693 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCMV-Tol2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5693)
AUTHORS Balciunas D, Wangensteen KJ, Wilber A, Bell J, Geurts A, Sivasubbu
S, Wang X, Hackett PB, Largaespada DA, McIvor RS, Ekker SC.
TITLE Harnessing a high cargo-capacity transposon for genetic applications
in vertebrates.
JOURNAL PLoS Genet. 2006 Nov 10;2(11):e169. Epub 2006 Aug 28.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pCMV-Tol2|
FEATURES Location/Qualifiers
source 1..5693
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 26..329
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 330..533
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 484..504
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 530..554
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 671..767
/label=SV40 intron
/note="modified SV40 intron with splice donor and acceptor
sites"
regulatory 823..832
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
polyA_signal 2831..2965
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2855..2874)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2909..2928
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(3076..3092)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3076..3092)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3089..3111)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3100..3116
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3124..3154)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3169..3190
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3307..3324)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3478..4066)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3558..3577)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4237..5097)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4860..4879
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5098..5202)
/gene="bla"
/label=AmpR promoter
primer_bind 5270..5288
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(5326..5348)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 5448..5467
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5661..5683
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 5675..5692
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 5676..5692
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 aattcgagct tgcatgcctg caggtcgtta cataacttac ggtaaatggc ccgcctggct
61 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
121 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
181 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
241 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
301 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
361 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
421 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
481 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
541 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
601 gggaccgatc cagcctccgg actctagagg atccggtact cgaggaactg aaaaaccaga
661 aagttaactg gtaagtttag tctttttgtc ttttatttca ggtcccggat ccggtggtgg
721 tgcaaatcaa agaactgctc ctcagtggat gttgccttta cttctaggcc tgtacggaag
781 tgttacttct gctctaaaag ctgcggaatt gtacccgcgg ccgccaccat ggaggaagta
841 tgtgattcat cagcagctgc gagcagcaca gtccaaaatc agccacagga tcaagagcac
901 ccgtggccgt atcttcgcga attcttttct ttaagtggtg taaataaaga ttcattcaag
961 atgaaatgtg tcctctgtct cccgcttaat aaagaaatat cggccttcaa aagttcgcca
1021 tcaaacctaa ggaagcatat tgagagaatg cacccaaatt acctcaaaaa ctactctaaa
1081 ttgacagcac agaagagaaa gatcgggacc tccacccatg cttccagcag taagcaactg
1141 aaagttgact cagttttccc agtcaaacat gtgtctccag tcactgtgaa caaagctata
1201 ttaaggtaca tcattcaagg acttcatcct ttcagcactg ttgatctgcc atcatttaaa
1261 gagctgatta gtacactgca gcctggcatt tctgtcatta caaggcctac tttacgctcc
1321 aagatagctg aagctgctct gatcatgaaa cagaaagtga ctgctgccat gagtgaagtt
1381 gaatggattg caaccacaac ggattgttgg actgcacgta gaaagtcatt cattggtgta
1441 actgctcact ggatcaaccc tggaagtctt gaaagacatt ccgctgcact tgcctgcaaa
1501 agattaatgg gctctcatac ttttgaggta ctggccagtg ccatgaatga tatccactca
1561 gagtatgaaa tacgtgacaa ggttgtttgc acaaccacag acagtggttc caactttatg
1621 aaggctttca gagtttttgg tgtggaaaac aatgatatcg agactgaggc aagaaggtgt
1681 gaaagtgatg acactgattc tgaaggctgt ggtgagggaa gtgatggtgt ggaattccaa
1741 gatgcctcac gagtcctgga ccaagacgat ggcttcgaat tccagctacc aaaacatcaa
1801 aagtgtgcct gtcacttact taacctagtc tcaagcgttg atgcccaaaa agctctctca
1861 aatgaacact acaagaaact ctacagatct gtctttggca aatgccaagc tttatggaat
1921 aaaagcagcc gatcggctct agcagctgaa gctgttgaat cagaaagccg gcttcagctt
1981 ttaaggccaa accaaacgcg gtggaattca acttttatgg ctgttgacag aattcttcaa
2041 atttgcaaag aagcaggaga aggcgcactt cggaatatat gcacctctct tgaggttcca
2101 atgtttaatc cagcagaaat gctgttcttg acagagtggg ccaacacaat gcgtccagtt
2161 gcaaaagtac tcgacatctt gcaagcggaa acgaatacac agctggggtg gctgctgcct
2221 agtgtccatc agttaagctt gaaacttcag cgactccacc attctctcag gtactgtgac
2281 ccacttgtgg atgccctaca acaaggaatc caaacacgat tcaagcatat gtttgaagat
2341 cctgagatca tagcagctgc catccttctc cctaaatttc ggacctcttg gacaaatgat
2401 gaaaccatca taaaacgagg catggactac atcagagtgc atctggagcc tttggaccac
2461 aagaaggaat tggccaacag ttcatctgat gatgaagatt ttttcgcttc tttgaaaccg
2521 acaacacatg aagccagcaa agagttggat ggatatctgg cctgtgtttc agacaccagg
2581 gagtctctgc tcacgtttcc tgctatttgc agcctctcta tcaagactaa tacacctctt
2641 cccgcatcgg ctgcctgtga gaggcttttc agcactgcag gattgctttt cagccccaaa
2701 agagctaggc ttgacactaa caattttgag aatcagcttc tactgaagtt aaatctgagg
2761 ttttacaact ttgagtagac tagtctgaag ggcgaattct gcagatatcc atcacactgg
2821 cggccgcggg gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
2881 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
2941 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
3001 tcagggggag gtgtgggagg ttttttcgga tcctctagag tcgacctgca ggcatgcaag
3061 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
3121 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
3181 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
3241 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
3301 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
3361 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
3421 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
3481 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
3541 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
3601 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
3661 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
3721 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
3781 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
3841 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
3901 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
3961 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
4021 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
4081 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
4141 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
4201 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
4261 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
4321 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
4381 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
4441 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
4501 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
4561 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
4621 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
4681 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
4741 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
4801 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
4861 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
4921 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
4981 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
5041 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
5101 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
5161 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
5221 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
5281 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
5341 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
5401 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
5461 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
5521 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
5581 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
5641 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtg
//
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