| 质粒名称: | Su9-EGFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 23214 |
| 存储实验室: | David Chan |
|
载体骨架基本信息 |
|
| 载体名称: | 未知 |
| 载体出品公司: | Clontech |
| 空载体大小: | 5000bp |
| 完整质粒大小: | 4914bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | Kanamycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | Subunit 9 of the F0-ATPase |
| 物种来源: | 光合细菌 Rhodopseudomonas palustris |
| 基因突变: | contains AA 1-69 of subunit 9 |
| GenBank编号 | JN247409 |
| 标签/融合蛋白: | EGFP (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’测序引物 | CMV-F |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | Su9-EGFP was a gift from David Chan (Addgene plasmid # 23214 ; http://n2t.net/addgene:23214 ; RRID:Addgene_23214) |
| 参考文献部分: | Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. Chen H, Detmer SA, Ewald AJ, Griffin EE, Fraser SE, Chan DC. J Cell Biol. 2003 Jan 20. 160(2):189-200. 10.1083/jcb.200211046 PubMed 12527753 |
买家导航
Su9-EGFP载体质粒基本信息
Su9-EGFP质粒图谱载体图谱和Su9-EGFP载体序列质粒序列多克隆位点信息
Su9-EGFP质粒载体简介
Su9-EGFP质粒序列载体序列
LOCUS Su9-EGFP 4914 bp DNA circular SYN 14-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4914)
AUTHORS Chen H, Detmer SA, Ewald AJ, Griffin EE, Fraser SE, Chan DC
TITLE Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion
and are essential for embryonic development.
JOURNAL J Cell Biol. 2003 Jan 20. 160(2):189-200.
PUBMED 12527753
REFERENCE 2 (bases 1 to 4914)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Cell
Biol. 2003 Jan 20. 160(2):189-200."
COMMENT Sequence Label: Su9-EGFP
FEATURES Location/Qualifiers
source 1..4914
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 854..863
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS join(860..862,863..865,866..1579)
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS join(860..862,863..865,866..1576)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(905..926)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1166..1185)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1513..1534
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 1702..1823
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1739..1758)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1793..1812
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1830..2285)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1967..1988)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2179..2198
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2312..2416
/gene="bla"
/label=AmpR promoter
promoter 2418..2775
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 2418..2609
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(2441..2461)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2626..2761
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2810..3604
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 2810..3604
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2864..2883)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3474..3493
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3792..3811)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3836..3883
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4212..4800
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4701..4720
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attcaccatg gcctccactc gtgtcctcgc
661 ctctcgcctg gcctcccgga tggctgcttc cgccaaggtt gcccgccctg ctgtccgcgt
721 tgctcaggtc agcaagcgca ccatccagac tggctccccc ctccagaccc tcaagcgcac
781 ccagatgacc tccatcgtca acgccaccac ccgccaggct ttccagaagc gcgcctactc
841 ttccggatcc atcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
901 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
961 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
1021 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
1081 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
1141 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
1201 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
1261 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
1321 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
1381 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
1441 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
1501 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
1561 ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt
1621 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
1681 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
1741 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
1801 tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt
1861 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
1921 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
1981 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
2041 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
2101 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
2161 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
2221 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
2281 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
2341 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
2401 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
2461 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
2521 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
2581 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
2641 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
2701 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
2761 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
2821 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
2881 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
2941 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
3001 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
3061 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
3121 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
3181 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
3241 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
3301 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
3361 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
3421 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
3481 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
3541 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
3601 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
3661 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
3721 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
3781 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
3841 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
3901 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
3961 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
4021 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
4081 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
4141 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
4201 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
4261 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
4321 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
4381 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
4441 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
4501 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
4561 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
4621 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
4681 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
4741 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
4801 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
4861 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
