pLVX-IRES-Puro

价格:1350元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-IRES-Puro
质粒类型: 慢病毒载体;哺乳动物细胞表达载体;双顺反子载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8140 bp
5' 测序引物及序列: CMV-F: CGCAAATGGGCGGTAGGCGTG (Invitrogen)
3' 测序引物及序列: IRES-R: CCTCACATTGCCAAAAGACG
载体标签: 无标签
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
备注: pLVX-IRES-Puro载体以双顺反子的形式同时表达Puro抗性基因和目的基因;
CMV启动子驱动目的基因的过表达;
克隆宿主菌为Stbl3,在30度条件下进行质粒扩增。
产品目录号: 632183
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-IRES-Puro载体图谱



pLVX-IRES-Puro多克隆位点

pLVX-IRES-Puro载体特征

载体简介

载体序列

LOCUS       pLVX-IRES-Puro   8140 bp    DNA   circular             8-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632183,632186
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    2028..2151
                     /label=cPPT/CTS\(DNA\flap)
     promoter        2185..2787
                     /label=CMV\IE\Promoter
     misc_feature    4062..4653
                     /label=WPRE
     rep_origin      complement(5961..6634)
                     /label=pUC\origin
     CDS             complement(6779..7775)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             4856..5492
                     /label=3'\LTR
     misc_feature    2841..3448
                     /label=IRES
     CDS             3449..4048
                     /label=Puro(R)
     misc_feature    2803..2840
                     /label=MCS
BASE COUNT     2082 a      2022 c      2094 g      1942 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt 
     2821 tctagagcgg ccgcggatcc cgcccctctc cctccccccc ccctaacgtt actggccgaa 
     2881 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt 
     2941 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg 
     3001 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc 
     3061 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc 
     3121 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa 
     3181 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc 
     3241 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg 
     3301 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac 
     3361 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt 
     3421 gccacaaccc acaaggagac gaccttccat gaccgagtac aagcccacgg tgcgcctcgc 
     3481 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 
     3541 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga 
     3601 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 
     3661 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 
     3721 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 
     3781 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 
     3841 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 
     3901 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 
     3961 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 
     4021 gtgcatgacc cgcaagcccg gtgcctagac gcgtctggaa caatcaacct ctggattaca 
     4081 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 
     4141 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 
     4201 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 
     4261 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 
     4321 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 
     4381 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 
     4441 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 
     4501 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 
     4561 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 
     4621 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga 
     4681 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct 
     4741 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc 
     4801 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga 
     4861 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca 
     4921 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac 
     4981 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa 
     5041 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag 
     5101 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct 
     5161 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg 
     5221 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg 
     5281 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct 
     5341 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 
     5401 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 
     5461 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 
     5521 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt 
     5581 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 
     5641 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 
     5701 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 
     5761 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 
     5821 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 
     5881 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 
     5941 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 
     6001 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 
     6061 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 
     6121 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 
     6181 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 
     6241 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 
     6301 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 
     6361 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 
     6421 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 
     6481 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 
     6541 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 
     6601 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 
     6661 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 
     6721 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 
     6781 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
     6841 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
     6901 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 
     6961 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
     7021 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
     7081 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
     7141 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
     7201 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
     7261 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
     7321 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
     7381 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
     7441 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
     7501 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
     7561 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
     7621 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 
     7681 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 
     7741 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt 
     7801 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 
     7861 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 
     7921 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct 
     7981 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta 
     8041 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa 
     8101 gaaattgtat ttgttaaata tgtactacaa acttagtagt 
//

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