pLVX-Het-Mem1

价格:4000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Clontech
载体名称: pLVX-Het-Mem1
质粒类型: 慢病毒载体;双顺反子载体;信号通路载体;亚细胞定位载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 9268 bp
5' 测序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: DmrA
载体抗性: 氨苄青霉素
筛选标记: 潮霉素(Hygromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 慢病毒载体pLVX-Het-Mem1组成型表达DmrA融合蛋白;利用信号受体的二聚化作用研究细胞信号通路; DmrA蛋白的N端有膜定位信号。
产品目录号: 635075
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-Het-Mem1载体图谱



pLVX-Het-Mem1 多克隆位点

pLVX-Het-Mem1 载体特征

载体简介

载体序列

LOCUS       pLVX-Het-Mem1   9268 bp    DNA   circular             7-NOV-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No.635074, 635075
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    2028..2151
                     /label=CTS/cppt\(DNA\flap)
     promoter        2185..2788
                     /label=pCMV
     misc_feature    5190..5781
                     /label=WPRE
     rep_origin      complement(7091..7761)
                     /label=pUC\origin
     CDS             complement(7906..8902)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     LTR             5985..6621
                     /label=3'\LTR
     CDS             4142..5176
                     /label=Hyg(R)
     misc_feature    3543..4118
                     /label=IRES
     misc_feature    2822..2863
                     /label=N-myr\signal
     misc_feature    2864..3190
                     /label=DmrA
     misc_feature    3194..3517
                     /label=DmrA
BASE COUNT     2376 a      2276 c      2417 g      2199 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tagctagagg atctatttcc ggtgaattcc tcgagactag 
     2821 tatggggagt agcaagagca agcctaagga ccccagccag cgctctagag gagtgcaggt 
     2881 ggaaaccatc tccccaggag acgggcgcac cttccccaag cgcggccaga cctgcgtggt 
     2941 gcactacacc gggatgcttg aagatggaaa gaaatttgat tcctcccggg acagaaacaa 
     3001 gccctttaag tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc 
     3061 ccagatgagt gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc 
     3121 cactgggcac ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct 
     3181 aaaactggaa actagaggcg tgcaggtcga gaccatcagc cccggcgacg gccgcacctt 
     3241 tcccaagaga ggccagactt gcgtggtcca ctacaccggc atgctggagg acggcaagaa 
     3301 gttcgacagc agccgcgacc gcaacaagcc cttcaagttc atgctgggca aacaggaagt 
     3361 gatccgcggc tgggaggaag gcgtggctca gatgagcgtg gggcagcggg ccaagctgac 
     3421 catcagcccc gactatgcct acggcgccac cggccacccc ggcatcatcc ccccccacgc 
     3481 caccctggtg ttcgacgtgg agctgctgaa gctggagagc ggccgcggat cctacgtatg 
     3541 accgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc 
     3601 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg 
     3661 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca 
     3721 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa 
     3781 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt 
     3841 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt 
     3901 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca 
     3961 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc 
     4021 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc 
     4081 acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac ccgtaccaaa 
     4141 gatggataga tccggaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga 
     4201 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt 
     4261 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt 
     4321 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt 
     4381 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg 
     4441 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga 
     4501 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg 
     4561 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc 
     4621 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc 
     4681 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc 
     4741 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg 
     4801 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc 
     4861 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc 
     4921 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag 
     4981 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt 
     5041 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg 
     5101 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc 
     5161 gagggcaaag gaatagacgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa 
     5221 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 
     5281 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 
     5341 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 
     5401 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 
     5461 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 
     5521 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 
     5581 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 
     5641 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 
     5701 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 
     5761 tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc 
     5821 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga 
     5881 ggaggaggag gtgggttttt ccagtcacac ctcaggtacc tttaagacca atgacttaca 
     5941 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc 
     6001 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc 
     6061 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt 
     6121 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca 
     6181 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag 
     6241 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt 
     6301 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag 
     6361 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag 
     6421 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 
     6481 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 
     6541 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 
     6601 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 
     6661 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt taccgtcgac 
     6721 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 
     6781 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 
     6841 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 
     6901 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 
     6961 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
     7021 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 
     7081 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
     7141 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
     7201 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
     7261 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
     7321 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 
     7381 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
     7441 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
     7501 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
     7561 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 
     7621 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
     7681 tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 
     7741 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 
     7801 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 
     7861 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 
     7921 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 
     7981 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 
     8041 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 
     8101 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 
     8161 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 
     8221 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 
     8281 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 
     8341 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 
     8401 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 
     8461 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 
     8521 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 
     8581 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 
     8641 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 
     8701 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 
     8761 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 
     8821 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 
     8881 ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata 
     8941 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 
     9001 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga 
     9061 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca 
     9121 attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa 
     9181 agaaattgta tttgttaaat atgtactaca aacttagtag tttttaaaga aattgtattt 
     9241 gttaaatatg tactacaaac ttagtagt 
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng