pHelper

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Agilent
载体名称: pHelper, pAAV-Helper
质粒类型: 腺病毒相关载体
表达水平:
载体大小: 11635 bp
GenBank序列号: AF369965
载体标签: --
载体抗性: 氨苄
备注: 使用在AAV free  腺病毒基因表达载体系统中。
产品目录号: 240071
稳定性: /
组成型: --
病毒/非病毒: 腺病毒

载体质粒图谱和多克隆位点信息

pHelper载体图谱

pHelper载体特征

载体简介

载体序列

LOCUS       pHelper               11635 bp    DNA     circular SYN 22-JUL-2001
DEFINITION  Cloning vector pHelper, complete sequence.
ACCESSION   AF369965
VERSION     AF369965.1  GI:14994096
KEYWORDS    .
SOURCE      Cloning vector pHelper
  ORGANISM  Cloning vector pHelper
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Grafsky,A.J. III.
  TITLE     pHelper for use in the AAV Helper-Free System
  JOURNAL   Unpublished
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..11635
                     /organism="Cloning vector pHelper"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:161366"
                     /lab_host="Escherichia coli"
     misc_feature    complement(1..5336)
                     /note="similar to Adenovirus-5 E2A"
     misc_feature    complement(5336..8537)
                     /note="similar to Adenovirus-5 E4"
     misc_feature    complement(8537..9280)
                     /note="similar to Adenovirus-5 VA"
     rep_origin      complement(9315..10167)
                     /note="ColE1 origin"
     CDS             complement(10182..11042)
                     /note="confers ampicillin resistance"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AAK76423.1"
                     /db_xref="GI:14994097"
     rep_origin      11172..11627
                     /note="f1 origin"
ORIGIN      
        1 ggtacccaac tccatgctta acagtcccca ggtacagccc accctgcgtc gcaaccagga
       61 acagctctac agcttcctgg agcgccactc gccctacttc cgcagccaca gtgcgcagat
      121 taggagcgcc acttcttttt gtcacttgaa aaacatgtaa aaataatgta ctaggagaca
      181 ctttcaataa aggcaaatgt ttttatttgt acactctcgg gtgattattt accccccacc
      241 cttgccgtct gcgccgttta aaaatcaaag gggttctgcc gcgcatcgct atgcgccact
      301 ggcagggaca cgttgcgata ctggtgttta gtgctccact taaactcagg cacaaccatc
      361 cgcggcagct cggtgaagtt ttcactccac aggctgcgca ccatcaccaa cgcgtttagc
      421 aggtcgggcg ccgatatctt gaagtcgcag ttggggcctc cgccctgcgc gcgcgagttg
      481 cgatacacag ggttgcagca ctggaacact atcagcgccg ggtggtgcac gctggccagc
      541 acgctcttgt cggagatcag atccgcgtcc aggtcctccg cgttgctcag ggcgaacgga
      601 gtcaactttg gtagctgcct tcccaaaaag ggtgcatgcc caggctttga gttgcactcg
      661 caccgtagtg gcatcagaag gtgaccgtgc ccggtctggg cgttaggata cagcgcctgc
      721 atgaaagcct tgatctgctt aaaagccacc tgagcctttg cgccttcaga gaagaacatg
      781 ccgcaagact tgccggaaaa ctgattggcc ggacaggccg cgtcatgcac gcagcacctt
      841 gcgtcggtgt tggagatctg caccacattt cggccccacc ggttcttcac gatcttggcc
      901 ttgctagact gctccttcag cgcgcgctgc ccgttttcgc tcgtcacatc catttcaatc
      961 acgtgctcct tatttatcat aatgctcccg tgtagacact taagctcgcc ttcgatctca
     1021 gcgcagcggt gcagccacaa cgcgcagccc gtgggctcgt ggtgcttgta ggttacctct
     1081 gcaaacgact gcaggtacgc ctgcaggaat cgccccatca tcgtcacaaa ggtcttgttg
     1141 ctggtgaagg tcagctgcaa cccgcggtgc tcctcgttta gccaggtctt gcatacggcc
     1201 gccagagctt ccacttggtc aggcagtagc ttgaagtttg cctttagatc gttatccacg
     1261 tggtacttgt ccatcaacgc gcgcgcagcc tccatgccct tctcccacgc agacacgatc
     1321 ggcaggctca gcgggtttat caccgtgctt tcactttccg cttcactgga ctcttccttt
     1381 tcctcttgcg tccgcatacc ccgcgccact gggtcgtctt cattcagccg ccgcaccgtg
     1441 cgcttacctc ccttgccgtg cttgattagc accggtgggt tgctgaaacc caccatttgt
     1501 agcgccacat cttctctttc ttcctcgctg tccacgatca cctctgggga tggcgggcgc
     1561 tcgggcttgg gagaggggcg cttctttttc tttttggacg caatggccaa atccgccgtc
     1621 gaggtcgatg gccgcgggct gggtgtgcgc ggcaccagcg catcttgtga cgagtcttct
     1681 tcgtcctcgg actcgagacg ccgcctcagc cgcttttttg ggggcgcgcg gggaggcggc
     1741 ggcgacggcg acggggacga cacgtcctcc atggttggtg gacgtcgcgc cgcaccgcgt
     1801 ccgcgctcgg gggtggtttc gcgctgctcc tcttcccgac tggccatttc cttctcctat
     1861 aggcagaaaa agatcatgga gtcagtcgag aaggaggaca gcctaaccgc cccctttgag
     1921 ttcgccacca ccgcctccac cgatgccgcc aacgcgccta ccaccttccc cgtcgaggca
     1981 cccccgcttg aggaggagga agtgattatc gagcaggacc caggttttgt aagcgaagac
     2041 gacgaggatc gctcagtacc aacagaggat aaaaagcaag accaggacga cgcagaggca
     2101 aacgaggaac aagtcgggcg gggggaccaa aggcatggcg actacctaga tgtgggagac
     2161 gacgtgctgt tgaagcatct gcagcgccag tgcgccatta tctgcgacgc gttgcaagag
     2221 cgcagcgatg tgcccctcgc catagcggat gtcagccttg cctacgaacg ccacctgttc
     2281 tcaccgcgcg taccccccaa acgccaagaa aacggcacat gcgagcccaa cccgcgcctc
     2341 aacttctacc ccgtatttgc cgtgccagag gtgcttgcca cctatcacat ctttttccaa
     2401 aactgcaaga tacccctatc ctgccgtgcc aaccgcagcc gagcggacaa gcagctggcc
     2461 ttgcggcagg gcgctgtcat acctgatatc gcctcgctcg acgaagtgcc aaaaatcttt
     2521 gagggtcttg gacgcgacga gaaacgcgcg gcaaacgctc tgcaacaaga aaacagcgaa
     2581 aatgaaagtc actgtggagt gctggtggaa cttgagggtg acaacgcgcg cctagccgtg
     2641 ctgaaacgca gcatcgaggt cacccacttt gcctacccgg cacttaacct accccccaag
     2701 gttatgagca cagtcatgag cgagctgatc gtgcgccgtg cacgacccct ggagagggat
     2761 gcaaacttgc aagaacaaac cgaggagggc ctacccgcag ttggcgatga gcagctggcg
     2821 cgctggcttg agacgcgcga gcctgccgac ttggaggagc gacgcaagct aatgatggcc
     2881 gcagtgcttg ttaccgtgga gcttgagtgc atgcagcggt tctttgctga cccggagatg
     2941 cagcgcaagc tagaggaaac gttgcactac acctttcgcc agggctacgt gcgccaggcc
     3001 tgcaaaattt ccaacgtgga gctctgcaac ctggtctcct accttggaat tttgcacgaa
     3061 aaccgcctcg ggcaaaacgt gcttcattcc acgctcaagg gcgaggcgcg ccgcgactac
     3121 gtccgcgact gcgtttactt atttctgtgc tacacctggc aaacggccat gggcgtgtgg
     3181 cagcaatgcc tggaggagcg caacctaaag gagctgcaga agctgctaaa gcaaaacttg
     3241 aaggacctat ggacggcctt caacgagcgc tccgtggccg cgcacctggc ggacattatc
     3301 ttccccgaac gcctgcttaa aaccctgcaa cagggtctgc cagacttcac cagtcaaagc
     3361 atgttgcaaa actttaggaa ctttatccta gagcgttcag gaattctgcc cgccacctgc
     3421 tgtgcgcttc ctagcgactt tgtgcccatt aagtaccgtg aatgccctcc gccgctttgg
     3481 ggtcactgct accttctgca gctagccaac taccttgcct accactccga catcatggaa
     3541 gacgtgagcg gtgacggcct actggagtgt cactgtcgct gcaacctatg caccccgcac
     3601 cgctccctgg tctgcaattc gcaactgctt agcgaaagtc aaattatcgg tacctttgag
     3661 ctgcagggtc cctcgcctga cgaaaagtcc gcggctccgg ggttgaaact cactccgggg
     3721 ctgtggacgt cggcttacct tcgcaaattt gtacctgagg actaccacgc ccacgagatt
     3781 aggttctacg aagaccaatc ccgcccgcca aatgcggagc ttaccgcctg cgtcattacc
     3841 cagggccaca tccttggcca attgcaagcc atcaacaaag cccgccaaga gtttctgcta
     3901 cgaaagggac ggggggttta cctggacccc cagtccggcg aggagctcaa cccaatcccc
     3961 ccgccgccgc agccctatca gcagccgcgg gcccttgctt cccaggatgg cacccaaaaa
     4021 gaagctgcag ctgccgccgc cgccacccac ggacgaggag gaatactggg acagtcaggc
     4081 agaggaggtt ttggacgagg aggaggagat gatggaagac tgggacagcc tagacgaagc
     4141 ttccgaggcc gaagaggtgt cagacgaaac accgtcaccc tcggtcgcat tcccctcgcc
     4201 ggcgccccag aaattggcaa ccgttcccag catcgctaca acctccgctc ctcaggcgcc
     4261 gccggcactg cctgttcgcc gacccaaccg tagatgggac accactggaa ccagggccgg
     4321 taagtctaag cagccgccgc cgttagccca agagcaacaa cagcgccaag gctaccgctc
     4381 gtggcgcggg cacaagaacg ccatagttgc ttgcttgcaa gactgtgggg gcaacatctc
     4441 cttcgcccgc cgctttcttc tctaccatca cggcgtggcc ttcccccgta acatcctgca
     4501 ttactaccgt catctctaca gcccctactg caccggcggc agcggcagcg gcagcaacag
     4561 cagcggtcac acagaagcaa aggcgaccgg atagcaagac tctgacaaag cccaagaaat
     4621 ccacagcggc ggcagcagca ggaggaggag cgctgcgtct ggcgcccaac gaacccgtat
     4681 cgacccgcga gcttagaaat aggatttttc ccactctgta tgctatattt caacaaagca
     4741 ggggccaaga acaagagctg aaaataaaaa acaggtctct gcgctccctc acccgcagct
     4801 gcctgtatca caaaagcgaa gatcagcttc ggcgcacgct ggaagacgcg gaggctctct
     4861 tcagcaaata ctgcgcgctg actcttaagg actagtttcg cgccctttct caaatttaag
     4921 cgcgaaaact acgtcatctc cagcggccac acccggcgcc agcacctgtc gtcagcgcca
     4981 ttatgagcaa ggaaattccc acgccctaca tgtggagtta ccagccacaa atgggacttg
     5041 cggctggagc tgcccaagac tactcaaccc gaataaacta catgagcgcg ggaccccaca
     5101 tgatatcccg ggtcaacgga atccgcgccc accgaaaccg aattctcctc gaacaggcgg
     5161 ctattaccac cacacctcgt aataacctta atccccgtag ttggcccgct gccctggtgt
     5221 accaggaaag tcccgctccc accactgtgg tacttcccag agacgcccag gccgaagttc
     5281 agatgactaa ctcaggggcg cagcttgcgg gcggctttcg tcacagggtg cggtcgcccg
     5341 ggcgttttag ggcggagtaa cttgcatgta ttgggaattg tagttttttt aaaatgggaa
     5401 gtgacgtatc gtgggaaaac ggaagtgaag atttgaggaa gttgtgggtt ttttggcttt
     5461 cgtttctggg cgtaggttcg cgtgcggttt tctgggtgtt ttttgtggac tttaaccgtt
     5521 acgtcatttt ttagtcctat atatactcgc tctgtacttg gcccttttta cactgtgact
     5581 gattgagctg gtgccgtgtc gagtggtgtt ttttaatagg tttttttact ggtaaggctg
     5641 actgttatgg ctgccgctgt ggaagcgctg tatgttgttc tggagcggga gggtgctatt
     5701 ttgcctaggc aggagggttt ttcaggtgtt tatgtgtttt tctctcctat taattttgtt
     5761 atacctccta tgggggctgt aatgttgtct ctacgcctgc gggtatgtat tcccccgggc
     5821 tatttcggtc gctttttagc actgaccgat gttaaccaac ctgatgtgtt taccgagtct
     5881 tacattatga ctccggacat gaccgaggaa ctgtcggtgg tgctttttaa tcacggtgac
     5941 cagttttttt acggtcacgc cggcatggcc gtagtccgtc ttatgcttat aagggttgtt
     6001 tttcctgttg taagacaggc ttctaatgtt taaatgtttt tttttttgtt attttatttt
     6061 gtgtttaatg caggaacccg cagacatgtt tgagagaaaa atggtgtctt tttctgtggt
     6121 ggttccggaa cttacctgcc tttatctgca tgagcatgac tacgatgtgc ttgctttttt
     6181 gcgcgaggct ttgcctgatt ttttgagcag caccttgcat tttatatcgc cgcccatgca
     6241 acaagcttac ataggggcta cgctggttag catagctccg agtatgcgtg tcataatcag
     6301 tgtgggttct tttgtcatgg ttcctggcgg ggaagtggcc gcgctggtcc gtgcagacct
     6361 gcacgattat gttcagctgg ccctgcgaag ggacctacgg gatcgcggta tttttgttaa
     6421 tgttccgctt ttgaatctta tacaggtctg tgaggaacct gaatttttgc aatcatgatt
     6481 cgctgcttga ggctgaaggt ggagggcgct ctggagcaga tttttacaat ggccggactt
     6541 aatattcggg atttgcttag agacatattg ataaggtggc gagatgaaaa ttatttgggc
     6601 atggttgaag gtgctggaat gtttatagag gagattcacc ctgaagggtt tagcctttac
     6661 gtccacttgg acgtgagggc agtttgcctt ttggaagcca ttgtgcaaca tcttacaaat
     6721 gccattatct gttctttggc tgtagagttt gaccacgcca ccggagggga gcgcgttcac
     6781 ttaatagatc ttcattttga ggttttggat aatcttttgg aataaaaaaa aaaaaacatg
     6841 gttcttccag ctcttcccgc tcctcccgtg tgtgactcgc agaacgaatg tgtaggttgg
     6901 ctgggtgtgg cttattctgc ggtggtggat gttatcaggg cagcggcgca tgaaggagtt
     6961 tacatagaac ccgaagccag ggggcgcctg gatgctttga gagagtggat atactacaac
     7021 tactacacag agcgagctaa gcgacgagac cggagacgca gatctgtttg tcacgcccgc
     7081 acctggtttt gcttcaggaa atatgactac gtccggcgtt ccatttggca tgacactacg
     7141 accaacacga tctcggttgt ctcggcgcac tccgtacagt agggatcgcc tacctccttt
     7201 tgagacagag acccgcgcta ccatactgga ggatcatccg ctgctgcccg aatgtaacac
     7261 tttgacaatg cacaacgtga gttacgtgcg aggtcttccc tgcagtgtgg gatttacgct
     7321 gattcaggaa tgggttgttc cctgggatat ggttctgacg cgggaggagc ttgtaatcct
     7381 gaggaagtgt atgcacgtgt gcctgtgttg tgccaacatt gatatcatga cgagcatgat
     7441 gatccatggt tacgagtcct gggctctcca ctgtcattgt tccagtcccg gttccctgca
     7501 gtgcatagcc ggcgggcagg ttttggccag ctggtttagg atggtggtgg atggcgccat
     7561 gtttaatcag aggtttatat ggtaccggga ggtggtgaat tacaacatgc caaaagaggt
     7621 aatgtttatg tccagcgtgt ttatgagggg tcgccactta atctacctgc gcttgtggta
     7681 tgatggccac gtgggttctg tggtccccgc catgagcttt ggatacagcg ccttgcactg
     7741 tgggattttg aacaatattg tggtgctgtg ctgcagttac tgtgctgatt taagtgagat
     7801 cagggtgcgc tgctgtgccc ggaggacaag gcgtctcatg ctgcgggcgg tgcgaatcat
     7861 cgctgaggag accactgcca tgttgtattc ctgcaggacg gagcggcggc ggcagcagtt
     7921 tattcgcgcg ctgctgcagc accaccgccc tatcctgatg cacgattatg actctacccc
     7981 catgtaggcg tggacttccc cttcgccgcc cgttgagcaa ccgcaagttg gacagcagcc
     8041 tgtggctcag cagctggaca gcgacatgaa cttaagcgag ctgcccgggg agtttattaa
     8101 tatcactgat gagcgtttgg ctcgacagga aaccgtgtgg aatataacac ctaagaatat
     8161 gtctgttacc catgatatga tgctttttaa ggccagccgg ggagaaagga ctgtgtactc
     8221 tgtgtgttgg gagggaggtg gcaggttgaa tactagggtt ctgtgagttt gattaaggta
     8281 cggtgatcaa tataagctat gtggtggtgg ggctatacta ctgaatgaaa aatgacttga
     8341 aattttctgc aattgaaaaa taaacacgtt gaaacataac atgcaacagg ttcacgattc
     8401 tttattcctg ggcaatgtag gagaaggtgt aagagttggt agcaaaagtt tcagtggtgt
     8461 attttccact ttcccaggac catgtaaaag acatagagta agtgcttacc tcgctagttt
     8521 ctgtggattc actagaatcg atgtaggatg ttgcccctcc tgacgcggta ggagaagggg
     8581 agggtgccct gcatgtctgc cgctgctctt gctcttgccg ctgctgagga ggggggcgca
     8641 tctgccgcag caccggatgc atctgggaaa agcaaaaaag gggctcgtcc ctgtttccgg
     8701 aggaatttgc aagcggggtc ttgcatgacg gggaggcaaa cccccgttcg ccgcagtccg
     8761 gccggcccga gactcgaacc gggggtcctg cgactcaacc cttggaaaat aaccctccgg
     8821 ctacagggag cgagccactt aatgctttcg ctttccagcc taaccgctta cgccgcgcgc
     8881 ggccagtggc caaaaaagct agcgcagcag ccgccgcgcc tggaaggaag ccaaaaggag
     8941 cgctcccccg ttgtctgacg tcgcacacct gggttcgaca cgcgggcggt aaccgcatgg
     9001 atcacggcgg acggccggat ccggggttcg aaccccggtc gtccgccatg atacccttgc
     9061 gaatttatcc accagaccac ggaagagtgc ccgcttacag gctctccttt tgcacggtct
     9121 agagcgtcaa cgactgcgca cgcctcaccg gccagagcgt cccgaccatg gagcactttt
     9181 tgccgctgcg caacatctgg aaccgcgtcc gcgactttcc gcgcgcctcc accaccgccg
     9241 ccggcatcac ctggatgtcc aggtacatct acggattacg tcgacgttta aaccatatga
     9301 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9361 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9421 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9481 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9541 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9601 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9661 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9721 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9781 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9841 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     9901 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9961 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    10021 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    10081 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    10141 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    10201 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    10261 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    10321 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    10381 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    10441 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    10501 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    10561 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    10621 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10681 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    10741 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    10801 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10861 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10921 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10981 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    11041 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    11101 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    11161 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
    11221 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
    11281 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
    11341 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
    11401 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
    11461 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
    11521 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
    11581 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgatg gatcc
//

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