| 质粒名称: | YE1-BE4max-NG |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 138159 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pBR322/CMV |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | rAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI |
| 物种来源: | Synthetic; fusion of genes derived from R. norvegicus, S. pyogenes, and Bacillus phage PBS2 |
| 插入大小: | -- |
| 基因突变: | Within rAPOBEC1 W90Y + R126E; within Cas9 D10A + L1111R + D1135V + G1218R + E1219F + A1322R + R1335V + T1337R |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: |
NLS（插入件上的N端子）
NLS（插入件上的C端子）
|
| 克隆方法: | Ligation Independent克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | YE1-BE4max-NG was a gift from David Liu (Addgene plasmid # 138159 ; http://n2t.net/addgene:138159 ; RRID:Addgene_138159) |
| 参考文献部分: | Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Doman JL, Raguram A, Newby GA, Liu DR. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6. 10.1038/s41587-020-0414-6 PubMed 32042165 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS YE1-BE4max-NG 8961 bp ds-DNA circular SYN 19-FEB-2020
DEFINITION C-to-T base editor.
ACCESSION .
VERSION .
KEYWORDS YE1-BE4max-NG
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8961)
AUTHORS Doman JL, Raguram A, Newby GA, Liu DR
TITLE Evaluation and minimization of Cas9-independent off-target DNA
editing by cytosine base editors.
JOURNAL Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi:
10.1038/s41587-020-0414-6.
PUBMED 32042165
REFERENCE 2 (bases 1 to 8961)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 19, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8961
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 132..335
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 286..306
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 377..396
/label=T7
/note="T7 promoter, forward primer"
promoter 377..395
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 445..465
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1246..2961
/codon_start=1
/product="N-terminal portion of Streptococcus pyogenes Cas9
(Zetsche et al., 2015)"
/label=Cas9(N)
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
CDS 5377..5625
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 5656..5904
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 5947..5967
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5976..5993
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(6016..6033)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6022..6246
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(6317..6333)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6317..6333)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6330..6352)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6341..6357
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6365..6395)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6410..6431
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6548..6565)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6719..7307)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6799..6818)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7478..8338)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8101..8120
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8339..8443)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8522..8541)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 8713..131
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctcctc agagactggg
481 cctgtcgccg tcgatccaac cctgcgccgc cggattgaac ctcacgagtt tgaagtgttc
541 tttgaccccc gggagctgag aaaggagaca tgcctgctgt acgagatcaa ctggggaggc
601 aggcactcca tctggaggca cacctctcag aacacaaata agcacgtgga ggtgaacttc
661 atcgagaagt ttaccacaga gcggtacttc tgccccaata ccagatgtag catcacatgg
721 tttctgagct attccccttg cggagagtgt agcagggcca tcaccgagtt cctgtccaga
781 tatccacacg tgacactgtt tatctacatc gccaggctgt atcaccacgc agacccagag
841 aataggcagg gcctgcgcga tctgatcagc tccggcgtga ccatccagat catgacagag
901 caggagtccg gctactgctg gcggaacttc gtgaattatt ctcctagcaa cgaggcccac
961 tggcctaggt acccacacct gtgggtgcgc ctgtacgtgc tggagctgta ttgcatcatc
1021 ctgggcctgc ccccttgtct gaatatcctg cggagaaagc agccccagct gaccttcttt
1081 acaatcgccc tgcagtcttg tcactatcag aggctgccac cccacatcct gtgggccaca
1141 ggcctgaagt ctggaggatc tagcggagga tcctctggca gcgagacacc aggaacaagc
1201 gagtcagcaa caccagagag cagtggcggc agcagcggcg gcagcgacaa gaagtacagc
1261 atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
1321 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
1381 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
1441 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
1501 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
1561 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
1621 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
1681 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
1741 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
1801 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
1861 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
1921 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
1981 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
2041 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
2101 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
2161 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
2221 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
2281 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
2341 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
2401 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
2461 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
2521 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
2581 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
2641 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
2701 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
2761 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
2821 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
2881 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
2941 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
3001 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
3061 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
3121 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
3181 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
3241 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
3301 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
3361 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
3421 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
3481 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
3541 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
3601 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
3661 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
3721 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
3781 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
3841 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
3901 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
3961 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
4021 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
4081 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
4141 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
4201 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
4261 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
4321 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
4381 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
4441 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
4501 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
4561 aaagagtcta tcaggcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
4621 gaccctaaga agtacggcgg cttcgtcagc cccaccgtgg cctattctgt gctggtggtg
4681 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
4741 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
4801 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
4861 gaaaacggcc ggaagagaat gctggcctct gccagattcc tgcagaaggg aaacgaactg
4921 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
4981 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
5041 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
5101 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
5161 gagaatatca tccacctgtt taccctgacc aatctgggag cccctagggc cttcaagtac
5221 tttgacacca ccatcgaccg gaaggtgtac aggagcacca aagaggtgct ggacgccacc
5281 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
5341 ggtgacagcg gcgggagcgg cgggagcggg gggagcacta atctgagcga catcattgag
5401 aaggagactg ggaaacagct ggtcattcag gagtccatcc tgatgctgcc tgaggaggtg
5461 gaggaagtga tcggcaacaa gccagagtct gacatcctgg tgcacaccgc ctacgacgag
5521 tccacagatg agaatgtgat gctgctgacc tctgacgccc ccgagtataa gccttgggcc
5581 ctggtcatcc aggattctaa cggcgagaat aagatcaaga tgctgagcgg aggatccgga
5641 ggatctggag gcagcaccaa cctgtctgac atcatcgaga aggagacagg caagcagctg
5701 gtcatccagg agagcatcct gatgctgccc gaagaagtcg aagaagtgat cggaaacaag
5761 cctgagagcg atatcctggt ccataccgcc tacgacgaga gtaccgacga aaatgtgatg
5821 ctgctgacat ccgacgcccc agagtataag ccctgggctc tggtcatcca ggattccaac
5881 ggagagaaca aaatcaaaat gctgtctggc ggctcaaaaa gaaccgccga cggcagcgaa
5941 ttcgagccca agaagaagag gaaagtctaa ccggtcatca tcaccatcac cattgagttt
6001 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
6061 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
6121 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
6181 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
6241 ctatggcttc tgaggcggaa agaaccagct ggggctcgat accgtcgacc tctagctaga
6301 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
6361 cacacaacat acgagccgga agcataaagt gtaaagccta gggtgcctaa tgagtgagct
6421 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
6481 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
6541 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
6601 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
6661 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
6721 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
6781 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
6841 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
6901 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
6961 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
7021 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
7081 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
7141 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
7201 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
7261 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
7321 tcttttctac ggggtctgac actcagtgga acgaaaactc acgttaaggg attttggtca
7381 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
7441 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
7501 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
7561 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
7621 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
7681 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
7741 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
7801 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
7861 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
7921 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
7981 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
8041 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
8101 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
8161 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
8221 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
8281 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
8341 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
8401 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
8461 tgccacctga cgtcgacgga tcgggagatc gatctcccga tcccctaggg tcgactctca
8521 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
8581 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
8641 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
8701 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
8761 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
8821 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
8881 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
8941 tggcagtaca tcaagtgtat c
//
