| 质粒名称: | pAAV2/rh10 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 112866 |
| 存储实验室: | James M. Wilson |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | AAV |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Rep2/Cap-rh10 |
| 物种来源: | AAV |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | Truncated P5 |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | KS primer |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAAV2/rh10 was a gift from James M. Wilson (Addgene plasmid # 112866 ; http://n2t.net/addgene:112866 ; RRID:Addgene_112866) |
买家导航
pAAV2rh10载体基本信息
pAAV2rh10载体图谱质粒图谱和多克隆位点信息
pAAV2rh10载体简介
pAAV2rh10载体序列
LOCUS pAAV2/rh10 7336 bp ds-DNA circular SYN 07-AUG-2018
DEFINITION AAV packaging plasmid expressing Rep/Cap genes.
ACCESSION .
VERSION .
KEYWORDS pAAV2/rh10
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7336)
TITLE AAV packaging plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7336)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 7, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7336
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 131..153
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 145..162
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 146..162
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 172..191
/label=T7
/note="T7 promoter, forward primer"
promoter 172..190
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 219..235
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 220..236
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4694..4714)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(4694..4712)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4733..4749)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4733..4749)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4746..4768)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4757..4773
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4781..4811)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4826..4847
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4964..4981)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5135..5723)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5215..5234)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5894..6754)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6517..6536
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6755..6859)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6885..4)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7022..7043)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 7234..7253
/label=F1ori-R
/note="F1 origin, reverse primer"
ORIGIN
1 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
61 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
121 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
181 tcactatagg gcgaattggg taccgggccc cccctcgatc gaggtcgacg gtatcggggg
241 agctcgcagg gtctccattt tgaagcggga ggtttgaacg cgcagccgcc atgccggggt
301 tttacgagat tgtgattaag gtccccagcg accttgacga gcatctgccc ggcatttctg
361 acagctttgt gaactgggtg gccgagaagg aatgggagtt gccgccagat tctgacatgg
421 atctgaatct gattgagcag gcacccctga ccgtggccga gaagctgcag cgcgactttc
481 tgacggaatg gcgccgtgtg agtaaggccc cggaggctct tttctttgtg caatttgaga
541 agggagagag ctacttccac atgcacgtgc tcgtggaaac caccggggtg aaatccatgg
601 ttttgggacg tttcctgagt cagattcgcg aaaaactgat tcagagaatt taccgcggga
661 tcgagccgac tttgccaaac tggttcgcgg tcacaaagac cagaaatggc gccggaggcg
721 ggaacaaggt ggtggatgag tgctacatcc ccaattactt gctccccaaa acccagcctg
781 agctccagtg ggcgtggact aatatggaac agtatttaag cgcctgtttg aatctcacgg
841 agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc gcagacgcag gagcagaaca
901 aagagaatca gaatcccaat tctgatgcgc cggtgatcag atcaaaaact tcagccaggt
961 acatggagct ggtcgggtgg ctcgtggaca aggggattac ctcggagaag cagtggatcc
1021 aggaggacca ggcctcatac atctccttca atgcggcctc caactcgcgg tcccaaatca
1081 aggctgcctt ggacaatgcg ggaaagatta tgagcctgac taaaaccgcc cccgactacc
1141 tggtgggcca gcagcccgtg gaggacattt ccagcaatcg gatttataaa attttggaac
1201 taaacgggta cgatccccaa tatgcggctt ccgtctttct gggatgggcc acgaaaaagt
1261 tcggcaagag gaacaccatc tggctgtttg ggcctgcaac taccgggaag accaacatcg
1321 cggaggccat agcccacact gtgcccttct acgggtgcgt aaactggacc aatgagaact
1381 ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg ggaggagggg aagatgaccg
1441 ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag caaggtgcgc gtggaccaga
1501 aatgcaagtc ctcggcccag atagacccga ctcccgtgat cgtcacctcc aacaccaaca
1561 tgtgcgccgt gattgacggg aactcaacga ccttcgaaca ccagcagccg ttgcaagacc
1621 ggatgttcaa atttgaactc acccgccgtc tggatcatga ctttgggaag gtcaccaagc
1681 aggaagtcaa agactttttc cggtgggcaa aggatcacgt ggttgaggtg gagcatgaat
1741 tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc cagtgacgca gatataagtg
1801 agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac gtcagacgcg gaagcttcga
1861 tcaactacgc agacaggtac caaaacaaat gttctcgtca cgtgggcatg aatctgatgc
1921 tgtttccctg cagacaatgc gagagaatga atcagaattc aaatatctgc ttcactcacg
1981 gacagaaaga ctgtttagag tgctttcccg tgtcagaatc tcaacccgtt tctgtcgtca
2041 aaaaggcgta tcagaaactg tgctacattc atcatatcat gggaaaggtg ccagacgctt
2101 gcactgcctg cgatctggtc aatgtggatt tggatgactg catctttgaa caataaatga
2161 tttaaatcag gtatggctgc cgatggttat cttccagatt ggctcgagga caacctctct
2221 gagggcattc gcgagtggtg ggacttgaaa cctggagccc cgaaacccaa agccaaccag
2281 caaaagcagg acgacggccg gggtctggtg cttcctggct acaagtacct cggacccttc
2341 aacggactcg acaaggggga gcccgtcaac gcggcggacg cagcggccct cgagcacgac
2401 aaggcctacg accagcagct caaagcgggt gacaatccgt acctgcggta taaccacgcc
2461 gacgccgagt ttcaggagcg tctgcaagaa gatacgtctt ttgggggcaa cctcgggcga
2521 gcagtcttcc aggccaagaa gcgggttctc gaacctctcg gtctggttga ggaaggcgct
2581 aagacggctc ctggaaagaa gagaccggta gagccatcac cccagcgttc tccagactcc
2641 tctacgggca tcggcaagaa aggccagcag cccgcgaaaa agagactcaa ctttgggcag
2701 actggcgact cagagtcagt gcccgaccct caaccaatcg gagaaccccc cgcaggcccc
2761 tctggtctgg gatctggtac aatggctgca ggcggtggcg ctccaatggc agacaataac
2821 gaaggcgccg acggagtggg tagttcctca ggaaattggc attgcgattc cacatggctg
2881 ggcgacagag tcatcaccac cagcacccga acctgggccc tccccaccta caacaaccac
2941 ctctacaagc aaatctccaa cgggacttcg ggaggaagca ccaacgacaa cacctacttc
3001 ggctacagca ccccctgggg gtattttgac tttaacagat tccactgcca cttctcacca
3061 cgtgactggc agcgactcat caacaacaac tggggattcc ggcccaagag actcaacttc
3121 aagctcttca acatccaggt caaggaggtc acgcagaatg aaggcaccaa gaccatcgcc
3181 aataacctta ccagcacgat tcaggtcttt acggactcgg aataccagct cccgtacgtc
3241 ctcggctctg cgcaccaggg ctgcctgcct ccgttcccgg cggacgtctt catgattcct
3301 cagtacgggt acctgactct gaacaatggc agtcaggccg tgggccgttc ctccttctac
3361 tgcctggagt actttccttc tcaaatgctg agaacgggca acaactttga gttcagctac
3421 cagtttgagg acgtgccttt tcacagcagc tacgcgcaca gccaaagcct ggaccggctg
3481 atgaaccccc tcatcgacca gtacctgtac tacctgtctc ggactcagtc cacgggaggt
3541 accgcaggaa ctcagcagtt gctattttct caggccgggc ctaataacat gtcggctcag
3601 gccaaaaact ggctacccgg gccctgctac cggcagcaac gcgtctccac gacactgtcg
3661 caaaataaca acagcaactt tgcctggacc ggtgccacca agtatcatct gaatggcaga
3721 gactctctgg taaatcccgg tgtcgctatg gcaacccaca aggacgacga agagcgattt
3781 tttccgtcca gcggagtctt aatgtttggg aaacagggag ctggaaaaga caacgtggac
3841 tatagcagcg ttatgctaac cagtgaggaa gaaattaaaa ccaccaaccc agtggccaca
3901 gaacagtacg gcgtggtggc cgataacctg caacagcaaa acgccgctcc tattgtaggg
3961 gccgtcaaca gtcaaggagc cttacctggc atggtctggc agaaccggga cgtgtacctg
4021 cagggtccta tctgggccaa gattcctcac acggacggaa actttcatcc ctcgccgctg
4081 atgggaggct ttggactgaa acacccgcct cctcagatcc tgattaagaa tacacctgtt
4141 cccgcggatc ctccaactac cttcagtcaa gctaagctgg cgtcgttcat cacgcagtac
4201 agcaccggac aggtcagcgt ggaaattgaa tgggagctgc agaaagaaaa cagcaaacgc
4261 tggaacccag agattcaata cacttccaac tactacaaat ctacaaatgt ggactttgct
4321 gttaacacag atggcactta ttctgagcct cgccccatcg gcacccgtta cctcacccgt
4381 aatctgtaat tgcttgttaa tcaataaacc ggttgattcg tttcagttga actttggtct
4441 ctgcgaaggg cgaattcgtt taaacctgca ggactagagg tcctgtatta gaggtcacgt
4501 gagtgttttg cgacattttg cgacaccatg tggtcacgct gggtatttaa gcccgagtga
4561 gcacgcaggg tctccatttt gaagcgggag gtttgaacgc gcagccgcca agccgaattc
4621 tgcagatatc catcacactg gcggccgctc gactagagcg gccgccaccg cggtggagct
4681 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
4741 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
4801 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
4861 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
4921 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
4981 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
5041 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
5101 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
5161 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
5221 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
5281 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
5341 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
5401 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
5461 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
5521 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
5581 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
5641 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
5701 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
5761 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
5821 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
5881 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
5941 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
6001 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
6061 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
6121 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
6181 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
6241 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
6301 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
6361 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
6421 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
6481 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
6541 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
6601 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
6661 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
6721 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
6781 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
6841 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
6901 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
6961 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
7021 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
7081 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
7141 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
7201 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
7261 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
7321 tgcgccgcta cagggc
//
