pMSCVneo

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pMSCV-Neo载体基本信息

出品公司: Clontech
载体名称: pMSCVneo, pMSCV-neo, pMSCV neo
质粒类型: 哺乳动物细胞逆病毒基因表达载体
高拷贝/低拷贝: 低拷贝
启动子: LTR
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6436bp
5' 测序引物及序列: MSCV-F: CCCTTGAACCTCCTCGTTCGACC
3' 测序引物及序列: pMSCV-R: GAGACGTGCTACTTCCATTTGTC
载体标签: --
载体抗性: 氨苄
筛选标记: 新霉素(Neomycin)
备注: 逆病毒表达载体,优化后,有利于在造血干细胞中和癌细胞中进行基因表达。
产品目录号: 634401
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 逆病毒

pMSCV-Neo载体质粒图谱和多克隆位点信息

pMSCVneo载体图谱



pMSCVneo多克隆位点

RNAi-Ready pSIREN-RetroQ载体特征

pMSCV-Neo载体简介

pMSCV-Neo载体序列

LOCUS       pMSCVneo   6436 bp    DNA   circular             4-DEC-2009
COMMENT     Created by Clontech Laboratories Inc. 
COMMENT     Cat. No. 634401,631461
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     misc_signal     516..1405
                     /label=Extended\packaging\signal
     promoter        1429..1937
                     /label=Phosphoglycerate\Kinase\Promoter
     CDS             1951..2751
                     /label=Neo(R)
     LTR             2859..3342
                     /label=3'\PCMV\LTR
     LTR             1..515
                     /label=5'\MMSV\LTR
     CDS             complement(4645..5502)
                     /label=Amp(R)
     rep_origin      3882..3882
                     /label=pUC\origin
     splicing_signal 519..522
                     /label=Splice\Donor
     splicing_signal 1397..1399
                     /label=Splice\Acceptor
BASE COUNT     1458 a      1758 c      1708 g      1512 t 
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca 
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt 
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata 
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca 
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga 
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg 
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt 
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa 
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga 
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag 
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa 
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg 
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct 
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga 
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag 
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc 
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc 
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg 
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag 
     1381 ccctcactcc ttctctaggc gccggaattc gttaacctcg agagatctaa ttctaccggg 
     1441 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca 
     1501 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc 
     1561 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga 
     1621 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc 
     1681 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg 
     1741 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg 
     1801 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg 
     1861 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct 
     1921 catctccggg cctttcgacc tgcagccaat atgggatcgg ccattgaaca agatggattg 
     1981 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 
     2041 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 
     2101 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta 
     2161 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 
     2221 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 
     2281 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 
     2341 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 
     2401 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 
     2461 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc 
     2521 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 
     2581 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 
     2641 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 
     2701 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaggggat 
     2761 ccgtcgacct gcagccaagc ttatcgataa aataaaagat tttatttagt ctccagaaaa 
     2821 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt 
     2881 gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga 
     2941 gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 
     3001 gggccaagaa cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat 
     3061 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc 
     3121 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc 
     3181 ccacaacccc tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg 
     3241 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg 
     3301 tctcctctga gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga 
     3361 agttggagaa caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag 
     3421 ccactgtttt gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag 
     3481 aaagagctgg ggagaattgt gaaattgtta tccgctcaca attccacaca acatacgagc 
     3541 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 
     3601 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 
     3661 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 
     3721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
     3781 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 
     3841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
     3901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
     3961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
     4021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 
     4081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
     4141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
     4201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
     4261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
     4321 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
     4381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
     4441 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
     4501 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 
     4561 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 
     4621 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 
     4681 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 
     4741 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 
     4801 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 
     4861 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 
     4921 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 
     4981 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 
     5041 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 
     5101 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 
     5161 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 
     5221 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 
     5281 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 
     5341 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 
     5401 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 
     5461 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 
     5521 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 
     5581 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 
     5641 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 
     5701 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 
     5761 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 
     5821 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 
     5881 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 
     5941 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 
     6001 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 
     6061 gttttcccag tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg caaggagatg 
     6121 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc 
     6181 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca 
     6241 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggcgatta 
     6301 gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat 
     6361 caccagctga agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc 
     6421 cagaaaaagg ggggaa 
//

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pMSCV-Neo载体应用举例

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