pCMV-hA3H-BE3

LOCUS       pCMV-hA3H-BE3                8454 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses hA3H-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-hA3H-BE3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Wang X, Li J, Wang Y, Yang V, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol.,  2018
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        137..516
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        517..720
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     671..691
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     762..781
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        762..780
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      788..797
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1451..5551
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5564..5812
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5825..5845
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5854..5871
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5894..5911)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5900..6124
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6195..6211)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6195..6211)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6208..6230)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6219..6235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6243..6273)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6288..6309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6426..6443)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6597..7185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6677..6696)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7356..8216)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7979..7998
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8217..8321)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8400..8419)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
       61 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
      121 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
      181 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      241 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      301 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      361 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      421 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      481 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      541 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      601 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      661 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      721 ggtttagtga accgtcagat ccgctagaga tccgcggccg ctaatacgac tcactatagg
      781 gagagccgcc accatggctc tgttaacagc cgaaacattc cgcttacagt ttaacaacaa
      841 gcgccgcctc agaaggcctt actacccgag gaaggccctc ttgtgttacc agctgacgcc
      901 gcagaatggc tccacgccca cgagaggcta ctttgaaaac aagaaaaagt gccatgcaga
      961 aatttgcttt attaacgaga tcaagtccat gggactggac gaaacgcagt gctaccaagt
     1021 cacctgttac ctcacgtgga gcccctgctc ctcctgtgcc tgggagctgg ttgacttcat
     1081 caaggctcac gaccatctga acctgggcat cttcgcctcc cgcctgtact accactggtg
     1141 caagccccag cagaaggggc tgcggcttct gtgtggatcc caggtcccgg tggaggtcat
     1201 gggcttccca gagtttgctg actgctggga aaactttgtg gaccacgaga aaccgctttc
     1261 cttcaacccc tataagatgt tagaggagct agataaaaac agtcgagcca taaagcgacg
     1321 gcttgagagg ataaaggggc tccttcttgt tcttttagat tccaggggta cgtgcgcagg
     1381 gtcgttacat ggatatattg tgagcggcag cgagactccc gggacctcag agtccgccac
     1441 acccgaaagt gataaaaagt attctattgg tttagccatc ggcactaatt ccgttggatg
     1501 ggctgtcata accgatgaat acaaagtacc ttcaaagaaa tttaaggtgt tggggaacac
     1561 agaccgtcat tcgattaaaa agaatcttat cggtgccctc ctattcgata gtggcgaaac
     1621 ggcagaggcg actcgcctga aacgaaccgc tcggagaagg tatacacgtc gcaagaaccg
     1681 aatatgttac ttacaagaaa tttttagcaa tgagatggcc aaagttgacg attctttctt
     1741 tcaccgtttg gaagagtcct tccttgtcga agaggacaag aaacatgaac ggcaccccat
     1801 ctttggaaac atagtagatg aggtggcata tcatgaaaag tacccaacga tttatcacct
     1861 cagaaaaaag ctagttgact caactgataa agcggacctg aggttaatct acttggctct
     1921 tgcccatatg ataaagttcc gtgggcactt tctcattgag ggtgatctaa atccggacaa
     1981 ctcggatgtc gacaaactgt tcatccagtt agtacaaacc tataatcagt tgtttgaaga
     2041 gaaccctata aatgcaagtg gcgtggatgc gaaggctatt cttagcgccc gcctctctaa
     2101 atcccgacgg ctagaaaacc tgatcgcaca attacccgga gagaagaaaa atgggttgtt
     2161 cggtaacctt atagcgctct cactaggcct gacaccaaat tttaagtcga acttcgactt
     2221 agctgaagat gccaaattgc agcttagtaa ggacacgtac gatgacgatc tcgacaatct
     2281 actggcacaa attggagatc agtatgcgga cttatttttg gctgccaaaa accttagcga
     2341 tgcaatcctc ctatctgaca tactgagagt taatactgag attaccaagg cgccgttatc
     2401 cgcttcaatg atcaaaaggt acgatgaaca tcaccaagac ttgacacttc tcaaggccct
     2461 agtccgtcag caactgcctg agaaatataa ggaaatattc tttgatcagt cgaaaaacgg
     2521 gtacgcaggt tatattgacg gcggagcgag tcaagaggaa ttctacaagt ttatcaaacc
     2581 catattagag aagatggatg ggacggaaga gttgcttgta aaactcaatc gcgaagatct
     2641 actgcgaaag cagcggactt tcgacaacgg tagcattcca catcaaatcc acttaggcga
     2701 attgcatgct atacttagaa ggcaggagga tttttatccg ttcctcaaag acaatcgtga
     2761 aaagattgag aaaatcctaa cctttcgcat accttactat gtgggacccc tggcccgagg
     2821 gaactctcgg ttcgcatgga tgacaagaaa gtccgaagaa acgattactc catggaattt
     2881 tgaggaagtt gtcgataaag gtgcgtcagc tcaatcgttc atcgagagga tgaccaactt
     2941 tgacaagaat ttaccgaacg aaaaagtatt gcctaagcac agtttacttt acgagtattt
     3001 cacagtgtac aatgaactca cgaaagttaa gtatgtcact gagggcatgc gtaaacccgc
     3061 ctttctaagc ggagaacaga agaaagcaat agtagatctg ttattcaaga ccaaccgcaa
     3121 agtgacagtt aagcaattga aagaggacta ctttaagaaa attgaatgct tcgattctgt
     3181 cgagatctcc ggggtagaag atcgatttaa tgcgtcactt ggtacgtatc atgacctcct
     3241 aaagataatt aaagataagg acttcctgga taacgaagag aatgaagata tcttagaaga
     3301 tatagtgttg actcttaccc tctttgaaga tcgggaaatg attgaggaaa gactaaaaac
     3361 atacgctcac ctgttcgacg ataaggttat gaaacagtta aagaggcgtc gctatacggg
     3421 ctggggacga ttgtcgcgga aacttatcaa cgggataaga gacaagcaaa gtggtaaaac
     3481 tattctcgat tttctaaaga gcgacggctt cgccaatagg aactttatgc agctgatcca
     3541 tgatgactct ttaaccttca aagaggatat acaaaaggca caggtttccg gacaagggga
     3601 ctcattgcac gaacatattg cgaatcttgc tggttcgcca gccatcaaaa agggcatact
     3661 ccagacagtc aaagtagtgg atgagctagt taaggtcatg ggacgtcaca aaccggaaaa
     3721 cattgtaatc gagatggcac gcgaaaatca aacgactcag aaggggcaaa aaaacagtcg
     3781 agagcggatg aagagaatag aagagggtat taaagaactg ggcagccaga tcttaaagga
     3841 gcatcctgtg gaaaataccc aattgcagaa cgagaaactt tacctctatt acctacaaaa
     3901 tggaagggac atgtatgttg atcaggaact ggacataaac cgtttatctg attacgacgt
     3961 cgatcacatt gtaccccaat cctttttgaa ggacgattca atcgacaata aagtgcttac
     4021 acgctcggat aagaaccgag ggaaaagtga caatgttcca agcgaggaag tcgtaaagaa
     4081 aatgaagaac tattggcggc agctcctaaa tgcgaaactg ataacgcaaa gaaagttcga
     4141 taacttaact aaagctgaga ggggtggctt gtctgaactt gacaaggccg gatttattaa
     4201 acgtcagctc gtggaaaccc gccaaatcac aaagcatgtt gcacagatac tagattcccg
     4261 aatgaatacg aaatacgacg agaacgataa gctgattcgg gaagtcaaag taatcacttt
     4321 aaagtcaaaa ttggtgtcgg acttcagaaa ggattttcaa ttctataaag ttagggagat
     4381 aaataactac caccatgcgc acgacgctta tcttaatgcc gtcgtaggga ccgcactcat
     4441 taagaaatac ccgaagctag aaagtgagtt tgtgtatggt gattacaaag tttatgacgt
     4501 ccgtaagatg atcgcgaaaa gcgaacagga gataggcaag gctacagcca aatacttctt
     4561 ttattctaac attatgaatt tctttaagac ggaaatcact ctggcaaacg gagagatacg
     4621 caaacgacct ttaattgaaa ccaatgggga gacaggtgaa atcgtatggg ataagggccg
     4681 ggacttcgcg acggtgagaa aagttttgtc catgccccaa gtcaacatag taaagaaaac
     4741 tgaggtgcag accggagggt tttcaaagga atcgattctt ccaaaaagga atagtgataa
     4801 gctcatcgct cgtaaaaagg actgggaccc gaaaaagtac ggtggcttcg atagccctac
     4861 agttgcctat tctgtcctag tagtggcaaa agttgagaag ggaaaatcca agaaactgaa
     4921 gtcagtcaaa gaattattgg ggataacgat tatggagcgc tcgtcttttg aaaagaaccc
     4981 catcgacttc cttgaggcga aaggttacaa ggaagtaaaa aaggatctca taattaaact
     5041 accaaagtat agtctgtttg agttagaaaa tggccgaaaa cggatgttgg ctagcgccgg
     5101 agagcttcaa aaggggaacg aactcgcact accgtctaaa tacgtgaatt tcctgtattt
     5161 agcgtcccat tacgagaagt tgaaaggttc acctgaagat aacgaacaga agcaactttt
     5221 tgttgagcag cacaaacatt atctcgacga aatcatagag caaatttcgg aattcagtaa
     5281 gagagtcatc ctagctgatg ccaatctgga caaagtatta agcgcataca acaagcacag
     5341 ggataaaccc atacgtgagc aggcggaaaa tattatccat ttgtttactc ttaccaacct
     5401 cggcgctcca gccgcattca agtattttga cacaacgata gatcgcaaac gatacacttc
     5461 taccaaggag gtgctagacg cgacactgat tcaccaatcc atcacgggat tatatgaaac
     5521 tcggatagat ttgtcacagc ttgggggtga ctctggtggt tctactaatc tgtcagatat
     5581 tattgaaaag gagaccggta agcaactggt tatccaggaa tccatcctca tgctcccaga
     5641 ggaggtggaa gaagtcattg ggaacaagcc ggaaagcgat atactcgtgc acaccgccta
     5701 cgacgagagc accgacgaga atgtcatgct tctgactagc gacgcccctg aatacaagcc
     5761 ttgggctctg gtcatacagg atagcaacgg tgagaacaag attaagatgc tctctggtgg
     5821 ttctcccaag aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa
     5881 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     5941 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     6001 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     6061 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     6121 atggcttctg aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc
     6181 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6241 cacaacatac gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa
     6301 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6361 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6421 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6481 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6541 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6601 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6661 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6721 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6781 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6841 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6901 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6961 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7021 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7081 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7141 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7201 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7261 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7321 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7381 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7441 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7501 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7561 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7621 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7681 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7741 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7801 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7861 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7921 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7981 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8041 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8101 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8161 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8221 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8281 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8341 ccacctgacg tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt
     8401 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtg
//