| 质粒名称: | pCMV-ABE7.8 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 102917 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pBR322/CMV |
| 载体出品公司: | Liu lab |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3388 bp |
| 完整质粒大小: | 8727 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ABE7.8,TadA-linker(32aa)-TadA*(7.8)-linker(32aa)-nCas9-NLS |
| 物种来源: | Synthetic; TadA is from E.coli (synthetic gene block, mammalian codon optimized), Cas9 is S. pyogenes (mammalian codon optimized) |
| 插入大小: | 5328 bp |
| 基因突变: | see manuscript |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | NLS（插接件上的C端子） |
| 克隆方法: | Ligation Independent 克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | T7 |
| 3' 测序引物 | M13 |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-ABE7.8 was a gift from David Liu (Addgene plasmid # 102917 ; http://n2t.net/addgene:102917 ; RRID:Addgene_102917) |
| 参考文献部分: | Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. 10.1038/nature24644 PubMed 29160308 |
买家导航
pCMVABE7.8载体基本信息
pCMVABE7.8载体图谱质粒图谱和多克隆位点信息
pCMVABE7.8载体简介
pCMVABE7.8载体序列
LOCUS pCMV-ABE7.8 8727 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION expresses ABE7.8 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCMV-ABE7.8
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8727)
AUTHORS Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu
DR
TITLE Programmable base editing of A*T to G*C in genomic DNA without DNA
cleavage.
JOURNAL Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644.
Epub 2017 Oct 25.
PUBMED 29160308
REFERENCE 2 (bases 1 to 8727)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8727
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 683..4783
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 4796..4816
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4825..4842
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(4865..4882)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4871..5095
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(5166..5182)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5166..5182)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5179..5201)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5190..5206
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5214..5244)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5259..5280
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5397..5414)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5568..6156)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5648..5667)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6327..7187)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6950..6969
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7188..7292)
/gene="bla"
/label=AmpR promoter
primer_bind complement(7371..7390)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 7562..7941
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 7942..8145
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 8096..8116
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 8187..8206
/label=T7
/note="T7 promoter, forward primer"
promoter 8187..8205
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 ttcttctggt ggttctagcg gcagcgagac tcccgggacc tcagagtccg ccacacccga
61 aagttctggt ggttcttctg gtggttcttc cgaagtcgag ttttcccatg agtactggat
121 gagacacgca ttgactctcg caaagagggc tctcgatgaa cgcgaggtgc ccgtgggggc
181 agtactcgtg ctcaacaatc gcgtaatcgg cgaaggttgg aatagggcaa tcggactcca
241 cgaccccact gcacatgcgg aaatcatggc ccttcgacag ggagggcttg tgatgcagaa
301 ttatcgactt atcgatgcga cgctgtacgt cacgtttgaa ccttgcgtaa tgtgcgcggg
361 agctatgatt cactcccgca ttggacgagt tgtattcggt gttcgcaacg ccaagacggg
421 tgccgcaggt tcactgatgg acgtgctgca ttacccaggc atgaaccacc gggtagaaat
481 cacagaaggc atattggcgg acgaatgtaa cgcgctgttg tgttactttt ttcgcatgcg
541 gaggcaggtc tttaacgccc agaaaaaagc acaatcctct actgactctg gtggttcttc
601 tggtggttct agcggcagcg agactcccgg gacctcagag tccgccacac ccgaaagttc
661 tggtggttct tctggtggtt ctgataaaaa gtattctatt ggtttagcca tcggcactaa
721 ttccgttgga tgggctgtca taaccgatga atacaaagta ccttcaaaga aatttaaggt
781 gttggggaac acagaccgtc attcgattaa aaagaatctt atcggtgccc tcctattcga
841 tagtggcgaa acggcagagg cgactcgcct gaaacgaacc gctcggagaa ggtatacacg
901 tcgcaagaac cgaatatgtt acttacaaga aatttttagc aatgagatgg ccaaagttga
961 cgattctttc tttcaccgtt tggaagagtc cttccttgtc gaagaggaca agaaacatga
1021 acggcacccc atctttggaa acatagtaga tgaggtggca tatcatgaaa agtacccaac
1081 gatttatcac ctcagaaaaa agctagttga ctcaactgat aaagcggacc tgaggttaat
1141 ctacttggct cttgcccata tgataaagtt ccgtgggcac tttctcattg agggtgatct
1201 aaatccggac aactcggatg tcgacaaact gttcatccag ttagtacaaa cctataatca
1261 gttgtttgaa gagaacccta taaatgcaag tggcgtggat gcgaaggcta ttcttagcgc
1321 ccgcctctct aaatcccgac ggctagaaaa cctgatcgca caattacccg gagagaagaa
1381 aaatgggttg ttcggtaacc ttatagcgct ctcactaggc ctgacaccaa attttaagtc
1441 gaacttcgac ttagctgaag atgccaaatt gcagcttagt aaggacacgt acgatgacga
1501 tctcgacaat ctactggcac aaattggaga tcagtatgcg gacttatttt tggctgccaa
1561 aaaccttagc gatgcaatcc tcctatctga catactgaga gttaatactg agattaccaa
1621 ggcgccgtta tccgcttcaa tgatcaaaag gtacgatgaa catcaccaag acttgacact
1681 tctcaaggcc ctagtccgtc agcaactgcc tgagaaatat aaggaaatat tctttgatca
1741 gtcgaaaaac gggtacgcag gttatattga cggcggagcg agtcaagagg aattctacaa
1801 gtttatcaaa cccatattag agaagatgga tgggacggaa gagttgcttg taaaactcaa
1861 tcgcgaagat ctactgcgaa agcagcggac tttcgacaac ggtagcattc cacatcaaat
1921 ccacttaggc gaattgcatg ctatacttag aaggcaggag gatttttatc cgttcctcaa
1981 agacaatcgt gaaaagattg agaaaatcct aacctttcgc ataccttact atgtgggacc
2041 cctggcccga gggaactctc ggttcgcatg gatgacaaga aagtccgaag aaacgattac
2101 tccatggaat tttgaggaag ttgtcgataa aggtgcgtca gctcaatcgt tcatcgagag
2161 gatgaccaac tttgacaaga atttaccgaa cgaaaaagta ttgcctaagc acagtttact
2221 ttacgagtat ttcacagtgt acaatgaact cacgaaagtt aagtatgtca ctgagggcat
2281 gcgtaaaccc gcctttctaa gcggagaaca gaagaaagca atagtagatc tgttattcaa
2341 gaccaaccgc aaagtgacag ttaagcaatt gaaagaggac tactttaaga aaattgaatg
2401 cttcgattct gtcgagatct ccggggtaga agatcgattt aatgcgtcac ttggtacgta
2461 tcatgacctc ctaaagataa ttaaagataa ggacttcctg gataacgaag agaatgaaga
2521 tatcttagaa gatatagtgt tgactcttac cctctttgaa gatcgggaaa tgattgagga
2581 aagactaaaa acatacgctc acctgttcga cgataaggtt atgaaacagt taaagaggcg
2641 tcgctatacg ggctggggac gattgtcgcg gaaacttatc aacgggataa gagacaagca
2701 aagtggtaaa actattctcg attttctaaa gagcgacggc ttcgccaata ggaactttat
2761 gcagctgatc catgatgact ctttaacctt caaagaggat atacaaaagg cacaggtttc
2821 cggacaaggg gactcattgc acgaacatat tgcgaatctt gctggttcgc cagccatcaa
2881 aaagggcata ctccagacag tcaaagtagt ggatgagcta gttaaggtca tgggacgtca
2941 caaaccggaa aacattgtaa tcgagatggc acgcgaaaat caaacgactc agaaggggca
3001 aaaaaacagt cgagagcgga tgaagagaat agaagagggt attaaagaac tgggcagcca
3061 gatcttaaag gagcatcctg tggaaaatac ccaattgcag aacgagaaac tttacctcta
3121 ttacctacaa aatggaaggg acatgtatgt tgatcaggaa ctggacataa accgtttatc
3181 tgattacgac gtcgatcaca ttgtacccca atcctttttg aaggacgatt caatcgacaa
3241 taaagtgctt acacgctcgg ataagaaccg agggaaaagt gacaatgttc caagcgagga
3301 agtcgtaaag aaaatgaaga actattggcg gcagctccta aatgcgaaac tgataacgca
3361 aagaaagttc gataacttaa ctaaagctga gaggggtggc ttgtctgaac ttgacaaggc
3421 cggatttatt aaacgtcagc tcgtggaaac ccgccaaatc acaaagcatg ttgcacagat
3481 actagattcc cgaatgaata cgaaatacga cgagaacgat aagctgattc gggaagtcaa
3541 agtaatcact ttaaagtcaa aattggtgtc ggacttcaga aaggattttc aattctataa
3601 agttagggag ataaataact accaccatgc gcacgacgct tatcttaatg ccgtcgtagg
3661 gaccgcactc attaagaaat acccgaagct agaaagtgag tttgtgtatg gtgattacaa
3721 agtttatgac gtccgtaaga tgatcgcgaa aagcgaacag gagataggca aggctacagc
3781 caaatacttc ttttattcta acattatgaa tttctttaag acggaaatca ctctggcaaa
3841 cggagagata cgcaaacgac ctttaattga aaccaatggg gagacaggtg aaatcgtatg
3901 ggataagggc cgggacttcg cgacggtgag aaaagttttg tccatgcccc aagtcaacat
3961 agtaaagaaa actgaggtgc agaccggagg gttttcaaag gaatcgattc ttccaaaaag
4021 gaatagtgat aagctcatcg ctcgtaaaaa ggactgggac ccgaaaaagt acggtggctt
4081 cgatagccct acagttgcct attctgtcct agtagtggca aaagttgaga agggaaaatc
4141 caagaaactg aagtcagtca aagaattatt ggggataacg attatggagc gctcgtcttt
4201 tgaaaagaac cccatcgact tccttgaggc gaaaggttac aaggaagtaa aaaaggatct
4261 cataattaaa ctaccaaagt atagtctgtt tgagttagaa aatggccgaa aacggatgtt
4321 ggctagcgcc ggagagcttc aaaaggggaa cgaactcgca ctaccgtcta aatacgtgaa
4381 tttcctgtat ttagcgtccc attacgagaa gttgaaaggt tcacctgaag ataacgaaca
4441 gaagcaactt tttgttgagc agcacaaaca ttatctcgac gaaatcatag agcaaatttc
4501 ggaattcagt aagagagtca tcctagctga tgccaatctg gacaaagtat taagcgcata
4561 caacaagcac agggataaac ccatacgtga gcaggcggaa aatattatcc atttgtttac
4621 tcttaccaac ctcggcgctc cagccgcatt caagtatttt gacacaacga tagatcgcaa
4681 acgatacact tctaccaagg aggtgctaga cgcgacactg attcaccaat ccatcacggg
4741 attatatgaa actcggatag atttgtcaca gcttgggggt gactctggtg gttctcccaa
4801 gaagaagagg aaagtctaac cggtcatcat caccatcacc attgagttta aacccgctga
4861 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
4921 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
4981 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
5041 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
5101 gaggcggaaa gaaccagctg gggctcgata ccgtcgacct ctagctagag cttggcgtaa
5161 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
5221 cgagccggaa gcataaagtg taaagcctag ggtgcctaat gagtgagcta actcacatta
5281 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
5341 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
5401 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
5461 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
5521 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
5581 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
5641 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
5701 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
5761 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
5821 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
5881 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
5941 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
6001 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
6061 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
6121 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
6181 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
6241 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
6301 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
6361 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
6421 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
6481 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
6541 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
6601 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
6661 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
6721 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
6781 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
6841 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
6901 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
6961 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
7021 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
7081 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
7141 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
7201 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
7261 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
7321 gtcgacggat cgggagatcg atctcccgat cccctagggt cgactctcag tacaatctgc
7381 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
7441 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
7501 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
7561 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
7621 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
7681 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
7741 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
7801 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
7861 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
7921 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
7981 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
8041 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
8101 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
8161 cagatccgct agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat
8221 gtccgaagtc gagttttccc atgagtactg gatgagacac gcattgactc tcgcaaagag
8281 ggcttgggat gaacgcgagg tgcccgtggg ggcagtactc gtgcataaca atcgcgtaat
8341 cggcgaaggt tggaataggc cgatcggacg ccacgacccc actgcacatg cggaaatcat
8401 ggcccttcga cagggagggc ttgtgatgca gaattatcga cttatcgatg cgacgctgta
8461 cgtcacgctt gaaccttgcg taatgtgcgc gggagctatg attcactccc gcattggacg
8521 agttgtattc ggtgcccgcg acgccaagac gggtgccgca ggttcactga tggacgtgct
8581 gcatcaccca ggcatgaacc accgggtaga aatcacagaa ggcatattgg cggacgaatg
8641 tgcggcgctg ttgtccgact tttttcgcat gcggaggcag gagatcaagg cccagaaaaa
8701 agcacaatcc tctactgact ctggtgg
//
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