| 出品公司: | Stratagene |
|---|---|
| 载体名称: | p53-Luc |
| 质粒类型: | 信号通路报告系统载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5.9kb |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
买家导航
p53-Luc载体质粒基本信息
p53-Luc质粒图谱载体图谱和p53-Luc载体序列质粒序列多克隆位点信息
p53-Luc质粒载体简介
p53-Luc质粒序列载体序列
LOCUS p53-Luc 5914 bp DNA circular SYN 19-DEC-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5914)
AUTHORS DotmaticsRD
TITLE Direct Submission
JOURNAL Exported Dec 19, 2025 from SnapGene 8.1.0
https://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5914
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 32..136
/gene="bla"
/label=AmpR promoter
CDS join(137..205,206..997)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
rep_origin 1168..1755
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 1934..2074
/label=bom
/note="basis of mobility region from pBR322"
polyA_signal 2313..2447
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
polyA_signal 2541..2673
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS 3030..4682
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/label=luciferase
intron 4813..4878
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 5008..5028
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 5453..5587
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
RBS 5622..5630
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
1561 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
1741 gcggagctat ggaaaaacgc cagcaacgcg cctttttacg gttcctggcc ttttgctggc
1801 cttttgctca catgttcttt cctgcgttat ccctgattct gtggataacc gtattaccgc
1861 ctttgagtgc tgataccgct cgccgcagcc gaacgaccga gcgcaagtca gtgagcgagg
1921 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
1981 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
2041 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
2101 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
2161 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggatca
2221 gccatatcac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
2281 acctgaaaca taaaatgaat gcaattgttg ttaacttgtt tattgcagct tataatggtt
2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
2401 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcagc catatcacat
2461 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
2521 aaatgaatgc aattgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
2581 atagcatcac aaatttcaca aataaagcaa tttttcactg cattctattg tggtttgtcc
2641 aaactcatca atgtatctta tcatgtctgg atccaagctc ctgcctggac ttgcctggcc
2701 tgcctggact tgcctggcct gcctggactt gcctgcctgg acttgcctgg cctgcctgga
2761 cttgcctggc ctgcctggac ttgcctggcc tgcctggact tgcctggcct gcctggactt
2821 gcctggcctg cctggacttg cctggcctgc ctggacttgc ctggcctgcc tggacttgcc
2881 tggcctgcct ggacttgcct ggcctgcctg gacttgcctg gcctgcctgg acttgcctgg
2941 cctgcctgga cttgcctggg cggaggactc tagaggggta tataatggaa gctcgaattc
3001 cagcttggca ttccggtact gttggtaaaa tggaagacgc caaaaacata aagaaaggcc
3061 cggcgccatt ctatcctcta gaggatggaa ccgctggaga gcaactgcat aaggctatga
3121 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtgaaca
3181 tcacgtacgc ggaatacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg
3241 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc
3301 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg
3361 aacgtgaatt gctcaacagt atgaacattt cgcagcctac cgtagtgttt gtttccaaaa
3421 aggggttgca aaaaattttg aacgtgcaaa aaaaattacc aataatccag aaaattatta
3481 tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc
3541 atctacctcc cggttttaat gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa
3601 caattgcact gataatgaat tcctctggat ctactgggtt acctaagggt gtggcccttc
3661 cgcatagaac tgcctgcgtc agattctcgc atgccagaga tcctattttt ggcaatcaaa
3721 tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta
3781 ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag
3841 agctgttttt acgatccctt caggattaca aaattcaaag tgcgttgcta gtaccaaccc
3901 tattttcatt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg
3961 aaattgcttc tgggggcgca cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct
4021 tccatcttcc agggatacga caaggatatg ggctcactga gactacatca gctattctga
4081 ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag
4141 cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcagaga ggcgaattat
4201 gtgtcagagg acctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct
4261 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac
4321 acttcttcat agttgaccgc ttgaagtctt taattaaata caaaggatat caggtggccc
4381 ccgctgaatt ggaatcgata ttgttacaac accccaacat cttcgacgcg ggcgtggcag
4441 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa
4501 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa
4561 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg
4621 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag tccaaattgt
4681 aaaatgtaac tgtattcagc gatgacgaaa ttcttagcta ttgtaatact ctagaggatc
4741 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
4801 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
4861 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
4921 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
4981 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
5041 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
5101 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
5161 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
5221 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
5281 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
5341 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
5401 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
5461 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
5521 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
5581 ctggatcccc aggaagctcc tctgtgtcct cataaaccct aacctcctta cttgagagga
5641 cattccaatc ataggctgcc catccaccct ctgtgtcctc ctgttaatta ggtcacttaa
5701 caaaaaggaa attgggtagg ggtttttcac agaccgcttt ctaaggggta attttaaaat
5761 atctgggaag tcccttccac tgctgtgttc cagaagtgtt ggtaaacagc ccacaaatgt
5821 caacagcaga aacatacaag ctgtcacttt gcacaaaggg cctcgtgata cgcctatttt
5881 tataggttaa tgtcatgata ataatggttt ctta
//
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p53-Luc质粒载体应用举例
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