pLVX-Tet3G

价格:1500元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Clontech
载体名称: pLVX-Tet3G , pLVX Tet3G
质粒类型: 慢病毒表达载体;四环素诱导系统;(shRNA)RNA干扰载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: --
载体大小: 9051 bp
5' 测序引物及序列: CMV-F: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签:
载体抗性: 氨苄青霉素
筛选标记: 新霉素(Neomycin)
备注: 编码Tet-On 3G反式激活物蛋白因子,诱导基因表达;
pLVX-Tet3G是四环素诱导系统的调控载体,对应的应答载体为pLVX-TRE3G。
产品目录号: 631187
稳定性: 稳表达
组成型: 诱导型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-Tet3G载体图谱


pLVX-Tet3G载体特征

载体简介

载体序列

LOCUS       pLVX-Tet3G   9051 bp    DNA   circular            10-AUG 2012 
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT	    631191, 631187, 631358, 631349, 631350, 631351, 635086  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|       
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=Packaging\signal
     misc_feature    1303..1536
                     /label=RRE
     misc_feature    2028..2151
                     /label=cPPT
     misc_feature    3576..4149
                     /label=IRES
     CDS             4162..4956
                     /label=Neo(R)
     misc_feature    4970..5561
                     /label=WPRE
     LTR             5765..6401
                     /label=3'\LTR
     misc_feature    complement(6871..7544)
                     /label=pUC\ori
     CDS             2821..3567
                     /label=Tet-On\3G
     misc_feature    complement(7689..8549)
                     /label=Amp(R)
     misc_feature    2185..2787
                     /label=pCMV
BASE COUNT     2318 a      2220 c      2322 g      2191 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgaggccacc 
     2821 atgtctagac tggacaagag caaagtcata aactctgctc tggaattact caatggagtc 
     2881 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc 
     2941 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg 
     3001 gacaggcatc atacccactc ctgccccctg gaaggcgagt catggcaaga ctttctgcgg 
     3061 aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat 
     3121 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg 
     3181 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt 
     3241 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca 
     3301 cctaccaccg attctatgcc cccacttctg aaacaagcaa ttgagctgtt cgaccggcag 
     3361 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag 
     3421 ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc 
     3481 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat 
     3541 tttgaccttg acatgctccc cgggtaagga tcccgcccct ctccctcccc cccccctaac 
     3601 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc 
     3661 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg 
     3721 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg 
     3781 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc 
     3841 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa 
     3901 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa 
     3961 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta 
     4021 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg 
     4081 aggttaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac 
     4141 gatgataagc ttgccacaac catggctgaa caagatggat tgcacgcagg ttctccggcc 
     4201 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 
     4261 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 
     4321 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg 
     4381 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 
     4441 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 
     4501 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 
     4561 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 
     4621 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 
     4681 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 
     4741 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 
     4801 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 
     4861 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 
     4921 atcgccttct atcgccttct tgacgagttc ttctgaacgc gtctggaaca atcaacctct 
     4981 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 
     5041 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 
     5101 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 
     5161 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 
     5221 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 
     5281 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 
     5341 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc 
     5401 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 
     5461 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 
     5521 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga 
     5581 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt 
     5641 gcctggctag aagcacaaga ggaggaggag gtgggttttt ccagtcacac ctcaggtacc 
     5701 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag 
     5761 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta 
     5821 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata 
     5881 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga 
     5941 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga 
     6001 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc 
     6061 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact 
     6121 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct 
     6181 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga 
     6241 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 
     6301 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 
     6361 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 
     6421 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt 
     6481 gctagcgttt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 
     6541 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 
     6601 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 
     6661 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 
     6721 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 
     6781 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 
     6841 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 
     6901 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 
     6961 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 
     7021 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 
     7081 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 
     7141 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 
     7201 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 
     7261 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 
     7321 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 
     7381 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 
     7441 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 
     7501 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 
     7561 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 
     7621 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 
     7681 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 
     7741 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 
     7801 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 
     7861 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 
     7921 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 
     7981 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 
     8041 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 
     8101 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 
     8161 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 
     8221 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 
     8281 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 
     8341 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 
     8401 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 
     8461 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 
     8521 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 
     8581 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 
     8641 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat 
     8701 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 
     8761 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 
     8821 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac 
     8881 cccataccct attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt 
     8941 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag 
     9001 tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag t 
//

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