PABE-5

价格:1200元

联系方式:I47-825O-882O

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PABE5载体基本信息

质粒名称: PABE-5 
出品公司: 爱迪基因
目录编号: 115626
存储实验室: Caixia Gao

载体骨架基本信息
载体名称: pJIT163
载体出品公司: --
载体抗性: 氨苄青霉素
空载体大小: --
完整质粒大小: --
载体修饰: --
载体类型: 植物表达,CRISPR
筛选标记: --
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: NLS-nCas9-NLS-wtTadA-TadA7.10-NLS
物种来源: --
插入大小: --
基因突变: --
GenBank编号 --
启动子 --
标签/融合蛋白: --
克隆方法: 限制性内切酶
5' 克隆位点: --
3' 克隆位点: --
5' 测序引物 --
3' 测序引物 --

文献引用方法
材料和方法部分: PABE-5 was a gift from Caixia Gao (Addgene plasmid # 115626 ; http://n2t.net/addgene:115626 ; RRID:Addgene_115626)
参考文献部分: Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. 10.1186/s13059-018-1443-z PubMed 29807545

PABE5载体质粒图谱和多克隆位点信息

PABE-5质粒图谱

PABE5载体简介

PABE5载体序列

LOCUS       PABE-5               10362 bp ds-DNA     circular SYN 10-JAN-2019
DEFINITION  Targeted A to G in wheat plants or monocotyledons protoplasts .
ACCESSION   .
VERSION     .
KEYWORDS    PABE-5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10362)
  AUTHORS   Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C
  TITLE     Expanded base editing in rice and wheat using a Cas9-adenosine 
            deaminase fusion.
  JOURNAL   Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.
  PUBMED    29807545
REFERENCE   2  (bases 1 to 10362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 10, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10362
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             996..1016
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1029..5129
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             5042..5050
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5136..5156
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6345..6392
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    6450..6626
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(7180..7196)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7180..7196)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7193..7215)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7204..7220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7228..7258)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7273..7294
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7411..7428)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7582..8170)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7662..7681)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8341..9201)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8964..8983
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9202..9306)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctctctacc ttctctagat cggcgttccg gtccatggtt agggcccggt agttctactt
       61 ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca
      121 cggatgcgac ctgtacgtca gacacgttct gattgctaac ttgccagtgt ttctctttgg
      181 ggaatcctgg gatggctcta gccgttccgc agacgggatc gatttcatga ttttttttgt
      241 ttcgttgcat agggtttggt ttgccctttt cctttatttc aatatatgcc gtgcacttgt
      301 ttgtcgggtc atcttttcat gctttttttt gtcttggttg tgatgatgtg gtctggttgg
      361 gcggtcgttc tagatcggag tagaattaat tctgtttcaa actacctggt ggatttatta
      421 attttggatc tgtatgtgtg tgccatacat attcatagtt acgaattgaa gatgatggat
      481 ggaaatatcg atctaggata ggtatacatg ttgatgcggg ttttactgat gcatatacag
      541 agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt
      601 tctagatcgg agtagaatac tgtttcaaac tacctggtgt atttattaat tttggaactg
      661 tatgtgtgtg tcatacatct tcatagttac gagtttaaga tggatggaaa tatcgatcta
      721 ggataggtat acatgttgat gtgggtttta ctgatgcata tacatgatgg catatgcagc
      781 atctattcat atgctctaac cttgagtacc tatctattat aataaacaag tatgttttat
      841 aattattttg atcttgatat acttggatga tggcatatgc agcagctata tgtggatttt
      901 tttagccctg ccttcatacg ctatttattt gcttggtact gtttcttttg tcgatgctca
      961 ccctgttgtt tggtgttact tctgcaaagc ttatgccaaa aaagaagaga aaggtttcag
     1021 gcggctccga caagaagtac tcgatcggcc tcgccattgg gactaactct gttggctggg
     1081 ccgtgatcac cgacgagtac aaggtgccct caaagaagtt caaggtcctg ggcaacaccg
     1141 atcggcattc catcaagaag aatctcattg gcgctctcct gttcgacagc ggcgagacgg
     1201 ctgaggctac gcggctcaag cgcaccgccc gcaggcggta cacgcgcagg aagaatcgca
     1261 tctgctacct gcaggagatt ttctccaacg agatggcgaa ggttgacgat tctttcttcc
     1321 acaggctgga ggagtcattc ctcgtggagg aggataagaa gcacgagcgg catccaatct
     1381 tcggcaacat tgtcgacgag gttgcctacc acgagaagta ccctacgatc taccatctgc
     1441 ggaagaagct cgtggactcc acagataagg cggacctccg cctgatctac ctcgctctgg
     1501 cccacatgat taagttcagg ggccatttcc tgatcgaggg ggatctcaac ccggacaata
     1561 gcgatgttga caagctgttc atccagctcg tgcagacgta caaccagctc ttcgaggaga
     1621 accccattaa tgcgtcaggc gtcgacgcga aggctatcct gtccgctagg ctctcgaagt
     1681 ctcggcgcct cgagaacctg atcgcccagc tgccgggcga gaagaagaac ggcctgttcg
     1741 ggaatctcat tgcgctcagc ctggggctca cgcccaactt caagtcgaat ttcgatctcg
     1801 ctgaggacgc caagctgcag ctctccaagg acacatacga cgatgacctg gataacctcc
     1861 tggcccagat cggcgatcag tacgcggacc tgttcctcgc tgccaagaat ctgtcggacg
     1921 ccatcctcct gtctgatatt ctcagggtga acaccgagat tacgaaggct ccgctctcag
     1981 cctccatgat caagcgctac gacgagcacc atcaggatct gaccctcctg aaggcgctgg
     2041 tcaggcagca gctccccgag aagtacaagg agatcttctt cgatcagtcg aagaacggct
     2101 acgctgggta cattgacggc ggggcctctc aggaggagtt ctacaagttc atcaagccga
     2161 ttctggagaa gatggacggc acggaggagc tgctggtgaa gctcaatcgc gaggacctcc
     2221 tgaggaagca gcggacattc gataacggca gcatcccaca ccagattcat ctcggggagc
     2281 tgcacgctat cctgaggagg caggaggact tctacccttt cctcaaggat aaccgcgaga
     2341 agatcgagaa gattctgact ttcaggatcc cgtactacgt cggcccactc gctaggggca
     2401 actcccgctt cgcttggatg acccgcaagt cagaggagac gatcacgccg tggaacttcg
     2461 aggaggtggt cgacaagggc gctagcgctc agtcgttcat cgagaggatg acgaatttcg
     2521 acaagaacct gccaaatgag aaggtgctcc ctaagcactc gctcctgtac gagtacttca
     2581 cagtctacaa cgagctgact aaggtgaagt atgtgaccga gggcatgagg aagccggctt
     2641 tcctgtctgg ggagcagaag aaggccatcg tggacctcct gttcaagacc aaccggaagg
     2701 tcacggttaa gcagctcaag gaggactact tcaagaagat tgagtgcttc gattcggtcg
     2761 agatctctgg cgttgaggac cgcttcaacg cctccctggg gacctaccac gatctcctga
     2821 agatcattaa ggataaggac ttcctggaca acgaggagaa tgaggatatc ctcgaggaca
     2881 ttgtgctgac actcactctg ttcgaggacc gggagatgat cgaggagcgc ctgaagactt
     2941 acgcccatct cttcgatgac aaggtcatga agcagctcaa gaggaggagg tacaccggct
     3001 gggggaggct gagcaggaag ctcatcaacg gcattcggga caagcagtcc gggaagacga
     3061 tcctcgactt cctgaagagc gatggcttcg cgaaccgcaa tttcatgcag ctgattcacg
     3121 atgacagcct cacattcaag gaggatatcc agaaggctca ggtgagcggc cagggggact
     3181 cgctgcacga gcatatcgcg aacctcgctg gctcgccagc tatcaagaag gggattctgc
     3241 agaccgtgaa ggttgtggac gagctggtga aggtcatggg caggcacaag cctgagaaca
     3301 tcgtcattga gatggcccgg gagaatcaga ccacgcagaa gggccagaag aactcacgcg
     3361 agaggatgaa gaggatcgag gagggcatta aggagctggg gtcccagatc ctcaaggagc
     3421 acccggtgga gaacacgcag ctgcagaatg agaagctcta cctgtactac ctccagaatg
     3481 gccgcgatat gtatgtggac caggagctgg atattaacag gctcagcgat tacgacgtcg
     3541 atcatatcgt tccacagtca ttcctgaagg atgactccat tgacaacaag gtcctcacca
     3601 ggtcggacaa gaaccggggc aagtctgata atgttccttc agaggaggtc gttaagaaga
     3661 tgaagaacta ctggcgccag ctcctgaatg ccaagctgat cacgcagcgg aagttcgata
     3721 acctcacaaa ggctgagagg ggcgggctct ctgagctgga caaggcgggc ttcatcaaga
     3781 ggcagctggt cgagacacgg cagatcacta agcacgttgc gcagattctc gactcacgga
     3841 tgaacactaa gtacgatgag aatgacaagc tgatccgcga ggtgaaggtc atcaccctga
     3901 agtcaaagct cgtctccgac ttcaggaagg atttccagtt ctacaaggtt cgggagatca
     3961 acaattacca ccatgcccat gacgcgtacc tgaacgcggt ggtcggcaca gctctgatca
     4021 agaagtaccc aaagctcgag agcgagttcg tgtacgggga ctacaaggtt tacgatgtga
     4081 ggaagatgat cgccaagtcg gagcaggaga ttggcaaggc taccgccaag tacttcttct
     4141 actctaacat tatgaatttc ttcaagacag agatcactct ggccaatggc gagatccgga
     4201 agcgccccct catcgagacg aacggcgaga cgggggagat cgtgtgggac aagggcaggg
     4261 atttcgcgac cgtcaggaag gttctctcca tgccacaagt gaatatcgtc aagaagacag
     4321 aggtccagac tggcgggttc tctaaggagt caattctgcc taagcggaac agcgacaagc
     4381 tcatcgcccg caagaaggac tgggatccga agaagtacgg cgggttcgac agccccactg
     4441 tggcctactc ggtcctggtt gtggcgaagg ttgagaaggg caagtccaag aagctcaaga
     4501 gcgtgaagga gctgctgggg atcacgatta tggagcgctc cagcttcgag aagaacccga
     4561 tcgatttcct ggaggcgaag ggctacaagg aggtgaagaa ggacctgatc attaagctcc
     4621 ccaagtactc actcttcgag ctggagaacg gcaggaagcg gatgctggct tccgctggcg
     4681 agctgcagaa ggggaacgag ctggctctgc cgtccaagta tgtgaacttc ctctacctgg
     4741 cctcccacta cgagaagctc aagggcagcc ccgaggacaa cgagcagaag cagctgttcg
     4801 tcgagcagca caagcattac ctcgacgaga tcattgagca gatttccgag ttctccaagc
     4861 gcgtgatcct ggccgacgcg aatctggata aggtcctctc cgcgtacaac aagcaccgcg
     4921 acaagccaat cagggagcag gctgagaata tcattcatct cttcaccctg acgaacctcg
     4981 gcgcccctgc tgctttcaag tacttcgaca caactatcga tcgcaagagg tacacaagca
     5041 ctaaggaggt cctggacgcg accctcatcc accagtcgat taccggcctc tacgagacgc
     5101 gcatcgacct gtctcagctc gggggcgacg aattcccaaa gaagaagcgc aaggtctccg
     5161 gcggatcttc tggaggatct agcggctccg agacaccagg aacatccgaa tccgctacac
     5221 cagagtcttc tggaggatct agcggaggat cttccgaggt ggagttctct cacgagtatt
     5281 ggatgaggca cgctcttaca cttgctaaga gagcttggga cgaaagagaa gtgccagttg
     5341 gcgccgttct tgtgcataat aatagggtga tcggcgaggg ttggaataga ccaattggaa
     5401 ggcatgatcc aacagctcac gcagagatta tggctctcag acaaggcggc ctcgttatgc
     5461 agaactacag gctcattgac gctacactct acgtgacact cgaaccttgc gttatgtgcg
     5521 ccggagctat gattcattct aggattggca gggtcgtgtt tggagctagg gacgctaaaa
     5581 caggagccgc cggatctctt atggacgtgt tgcatcatcc aggcatgaac catagggtgg
     5641 agattacaga gggcattctt gcagacgagt gcgctgctct tctttccgat ttcttcagga
     5701 tgagaaggca ggagattaag gcccagaaga aggctcagtc ttctacagat agcggaggat
     5761 cttccggagg atctagcggc tccgagacac caggaacatc cgaaagcgct acaccagaat
     5821 ctagcggagg ctcttccgga ggatcttctg aagtggagtt ctcccacgag tattggatga
     5881 ggcacgctct tacacttgct aaaagggcta gggacgaaag ggaagttcca gttggagctg
     5941 ttctcgtgct caataacagg gtgattggcg agggttggaa tagagccatt ggactccatg
     6001 atccaacagc tcacgcagag attatggctc ttagacaagg cggcctcgtt atgcagaatt
     6061 acagactcat cgacgccaca ctctacgtta ccttcgaacc ttgcgttatg tgcgccggag
     6121 ctatgatcca ttctaggatt ggcagggtcg tgttcggcgt tagaaacgct aagacaggag
     6181 ctgcaggctc tcttatggac gttcttcatt acccaggcat gaatcataga gtggagatca
     6241 cagaaggcat tcttgcagac gagtgcgcag ctctcctttg ctatttcttc aggatgccga
     6301 ggcaagtttt caacgctcag aagaaggccc agtcttctac agataagaga ccagcagcta
     6361 caaagaaggc cggacaagct aagaagaaga agtaggaatt cggtacgctg aaatcaccag
     6421 tctctctcta caaatctatc tctctctatt ttctccataa ataatgtgtg agtagtttcc
     6481 cgataaggga aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc
     6541 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     6601 caaaatccag tactaaaatc cagatctcct aaagtcccta tagatctttg tcgtgaatat
     6661 aaaccagaca cgagacgact aaacctggag cccagacgcc gttcgaagct agaagtaccg
     6721 cttaggcagg aggccgttag ggaaaagatg ctaaggcagg gttggttacg ttgactcccc
     6781 cgtaggtttg gtttaaatat gatgaagtgg acggaaggaa ggaggaagac aaggaaggat
     6841 aaggttgcag gccctgtgca aggtaagaag atggaaattt gatagaggta cgctactata
     6901 cttatactat acgctaaggg aatgcttgta tttataccct atacccccta ataacccctt
     6961 atcaatttaa gaaataatcc gcataagccc ccgcttaaaa attggtatca gagccatgaa
     7021 taggtctatg accaaaactc aagaggataa aacctcacca aaatacgaaa gagttcttaa
     7081 ctctaaagat aaaagatctt tcaagatcaa aactagttcc ctcacaccgg tgacggggat
     7141 cgcatgcgat atctcgagat ctagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7201 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7261 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7321 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7381 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7441 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7501 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7561 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7621 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7681 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7741 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7801 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7861 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7921 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7981 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8041 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8101 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8161 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8221 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8281 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8341 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8401 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8461 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8521 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8581 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8641 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8701 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8761 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8821 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8881 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8941 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9001 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9061 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9121 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9181 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9241 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9301 tccgcgcaca tttccccgaa aagtgccacc tgccagtgcc aagctaattc gagctcggta
     9361 cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa gttataaaaa
     9421 attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat ctttatacat
     9481 atatttaaac tttactctac gaataatata atctatagta ctacaataat atcagtgttt
     9541 tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag tattttgaca
     9601 acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt tttgcaaata
     9661 gcttcaccta tataatactt catccatttt attagtacat ccatttaggg tttagggtta
     9721 atggttttta tagactaatt tttttagtac atctatttta ttctatttta gcctctaaat
     9781 taagaaaact aaaactctat tttagttttt ttatttaata atttagatat aaaatagaat
     9841 aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa actaaggaaa
     9901 catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac gagtctaacg
     9961 gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac ggcacggcat
    10021 ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt tggacttgct
    10081 ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc
    10141 ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc accgctcctt
    10201 cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca
    10261 acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg
    10321 gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc cc
//

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