| 质粒名称: | Tet-O-FUW-EGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 30130 |
| 存储实验室: | Marius Wernig |
|
载体骨架基本信息 |
|
| 载体名称: | Tet-O-FUW |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 8373 bp |
| 完整质粒大小: | 9101 bp |
| 载体类型: | 哺乳细胞表达，Lentiviral |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Enhanced green fluorescent protein |
| 物种来源: | A. victoria |
| 插入大小: | 728 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoR1(未破坏) |
| 3' 克隆位点: | EcoR1(未破坏) |
| 5' 测序引物 | LNCX |
| 3' 测序引物 | WPRE-R |
|
文献引用方法 |
|
| 材料和方法部分: | Tet-O-FUW-EGFP was a gift from Marius Wernig (爱迪基因 plasmid # 30130) |
| 参考文献部分: | Direct conversion of fibroblasts to functional neurons by defined factors. Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M. Nature. 2010 Feb 25. 463(7284):1035-41. 10.1038/nature08797 PubMed 20107439 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Tet-O-FUW-EGFP 9120 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Tet-O-FUW-EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9120)
AUTHORS Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M
TITLE Direct conversion of fibroblasts to functional neurons by defined
factors.
JOURNAL Nature. 2010 Feb 25. 463(7284):1035-41.
PUBMED 20107439
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|Tet-O-FUW-EGFP|
FEATURES Location/Qualifiers
source 1..9120
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 7..386
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 388..586
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 541..561
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 604..784
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 831..956
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1449..1682
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2209..2326
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2383..2653
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 2686..2724
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2721..2745
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 2825..2834
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2831..3550
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2876..2897)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3137..3156)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3484..3505
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 3581..4169
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3634..3654)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4052..4063)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4172..4188)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4173..4189)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 4694..4874
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(4900..4917)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4906..5130
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5176..5604
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5263..5282)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5473..5494
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5613..5633)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5618..5947
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5798..5933
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5860..5879
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 5995..6042
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 6061..6435
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 6565..6686
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6602..6621)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6656..6675
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6735..6751)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6735..6751)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6748..6770)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6759..6775
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6783..6813)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6828..6849
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6966..6983)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7137..7725)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7217..7236)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7896..8756)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8519..8538
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8757..8861)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8936..8955)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg
2701 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
2761 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa
2821 ttccgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
2881 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
2941 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
3001 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
3061 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
3121 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
3181 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
3241 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
3301 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
3361 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
3421 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
3481 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
3541 gtacaagtaa acgcgtgaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
3601 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
3661 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
3721 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
3781 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
3841 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
3901 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
3961 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
4021 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
4081 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
4141 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
4201 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
4261 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
4321 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
4381 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
4441 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
4501 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
4561 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
4621 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
4681 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
4741 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
4801 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
4861 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
4921 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
4981 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
5041 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
5101 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
5161 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
5221 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
5281 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
5341 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
5401 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
5461 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
5521 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
5581 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
5641 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
5701 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
5761 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
5821 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
5881 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
5941 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
6001 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
6061 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
6121 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
6181 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
6241 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
6301 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
6361 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
6421 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
6481 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
6541 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
6601 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
6661 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
6721 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
6781 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
6841 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
6901 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
6961 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
7021 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
7081 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
7141 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
7201 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
7261 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
7321 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
7381 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
7441 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
7501 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
7561 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
7621 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
7681 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
7741 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
7801 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
7861 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
7921 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
7981 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
8041 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
8101 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
8161 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
8221 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
8281 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
8341 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
8401 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
8461 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
8521 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
8581 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
8641 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
8701 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
8761 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
8821 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
8881 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
8941 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
9001 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
9061 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
//
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