pRetroX-PTuner2-C

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pRetroX-PTuner2-C
质粒类型: 逆病毒载体;双顺反子载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV IE
载体大小: 7356 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: DD tag (C-端)
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: DH5α , Stellar
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 逆病毒载体pRetroX-PTuner2-C载体表达C端DD标签融合蛋白;
DD标签是一个大小为12KD的FKBP (L106P)去稳定结构域,带有DD标签的目的蛋白将很快被降解;
向培养基中加入Shield1配体后,情况将发生逆转,细胞中目的蛋白的量则迅速提高。
产品目录号: 631099
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pRetroX-PTuner2-C载体图谱



pRetroX-PTuner2-C 多克隆位点

pRetroX-PTuner2-C 载体特征

载体简介

载体序列

LOCUS       pRetroX-PTuner2-C   7356 bp    DNA   circular            11-APR-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631099,631095
FEATURES             Location/Qualifiers
     misc_feature    1..727
                     /label=CMV/MSV\5'\LTR
     misc_feature    757..1566
                     /label=Psi-\Extended\packaging\signal
     misc_feature    4055..4487
                     /label=3'\MMLV\LTR\(deletion\in\U3)
     CDS             complement(6134..6994)
                     /label=Amp(R)
     rep_origin      complement(5374..5374)
                     /label=pUC\origin
     promoter        2742..3250
                     /label=PGK\Promoter
     CDS             3271..3870
                     /label=Puro(R)
     polyA_signal    4313..4328
                     /label=PolyA\signal
     rep_origin      4988..5053
                     /label=SV40\origin
     promoter        1582..2170
                     /label=CMV\IE\Promoter
                     /note="Human cytomegalovirus (CMV) immediate early promoter: Enhancer region: 59-465; TATA box: 554-560;Transcription start point: 583; C->G 

mutation to remove Sac I site: 569"
     promoter        4767..5034
                     /label=SV40\Promoter
     CDS             2247..2720
                     /label=DD-G
BASE COUNT     1661 a      2069 c      1917 g      1709 t 
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca 
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg 
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt 
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac 
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa 
     2161 ccgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag 
     2221 tcgacggtac cgcgggcccg ggatccatca gtctgattgc ggcgttagcg gtagatcacg 
     2281 ttatcggcat ggaaaacgcc atgccgtgga acctgcctgc cgatctcgcc tggtttaaac 
     2341 gcaacacctt aaataaaccc gtgattatgg gccgccatac ctgggaatca atcggtcgtc 
     2401 cgttgccagg acgcaaaaat attatcctca gcagtcaacc gagtacggac gatcgcgtaa 
     2461 cgtgggtgaa gtcggtggat gaagccatcg cggcgtgtgg tgacgtacca gaaatcatgg 
     2521 tgattggcgg cggtcgcgtc tatgaacagc tcctgccaaa agcgcaaaaa ctgtatctga 
     2581 cgcatatcga cgcagaagtg gaaggcgaca cccatttccc ggattacgag ccggatgact 
     2641 gggaatcggt attcagcgaa ttccacgatg ctgatgcgca gaactctcac agctattgct 
     2701 ttgagattct ggagcggcga tgagcggccg cgactctaga taattctacc gggtagggga 
     2761 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg 
     2821 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc 
     2881 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc 
     2941 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag 
     3001 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg 
     3061 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg 
     3121 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc 
     3181 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc 
     3241 gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc 
     3301 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 
     3361 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 
     3421 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 
     3481 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 
     3541 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 
     3601 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 
     3661 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 
     3721 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 
     3781 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 
     3841 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc 
     3901 gaaaggagcg cacgacccca tgcatcggcg tctcgatcga gatatcagtg gtccaggctc 
     3961 tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca tagataaaat 
     4021 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg 
     4081 gcaagctact agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa 
     4141 tagagaagtt cagatcaagg tcaggaacag atggaacagg gtcgatcgac cctagagaac 
     4201 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta 
     4261 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag 
     4321 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc 
     4381 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga 
     4441 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc 
     4501 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggta agctggctgc 
     4561 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc 
     4621 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt 
     4681 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtagatc 
     4741 cggctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga 
     4801 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc 
     4861 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc 
     4921 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 
     4981 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 
     5041 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac tggcttaact 
     5101 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca 
     5161 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc 
     5221 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 
     5281 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 
     5341 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 
     5401 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 
     5461 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 
     5521 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 
     5581 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 
     5641 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 
     5701 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 
     5761 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 
     5821 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 
     5881 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 
     5941 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 
     6001 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 
     6061 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 
     6121 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 
     6181 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 
     6241 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 
     6301 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 
     6361 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 
     6421 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg 
     6481 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 
     6541 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 
     6601 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 
     6661 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 
     6721 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa 
     6781 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 
     6841 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 
     6901 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 
     6961 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 
     7021 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 
     7081 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca 
     7141 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag 
     7201 aattagcttg gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct 
     7261 catgtccaac attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa 
     7321 ttacggggtc attagttcat agcccatata tggagt 
//

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