Tet-pLKO-neo

价格:1250元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品人: Dmitri Wiederschain (2009)
载体名称: Tet-pLKO-neo
质粒类型: 慢病毒载体;RNAi载体;四环素诱导载体;Tet-On载体
高拷贝/低拷贝: 低拷贝
克隆方法: 限制性内切酶;多克隆位点
启动子: --
载体大小: 10959 bp
5' 测序引物及序列: 5’ GGCAGGGATATTCACCATTATCGTTTCAGA 3’
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: Stbl3 感受态细胞 ,以维持质粒的稳定性。
宿主细胞(系): 哺乳动物细胞
备注:
其它筛选标记 :
                        Tet-pLKO-puro
 
产品目录号: #21916
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

Tet-pLKO-neo载体图谱

载体简介

载体序列

LOCUS       Exported               10959 bp ds-DNA     circular SYN 10-AUG-2016
DEFINITION  synthetic circular DNA
KEYWORDS    Tet-pLKO-neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10959)
  AUTHORS   Biofeng Lab.
FEATURES             Location/Qualifiers
     source          1..10959
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        106..210
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             211..1071
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2118..2139
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2154..2184
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2192..2208
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2216..2232
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2253..2271
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2299..2525
                     /note="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2526..2706
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2753..2878
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3371..3604
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     protein_bind    4170..4188
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4198..4216
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    6134..6251
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6300..6810
                     /note="PGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     intron          6819..7391
                     /note="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     promoter        7446..7464
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7475..8092
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /note="TetR"
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
     misc_feature    8130..8703
                     /note="IRES"
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             8710..9504
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    complement(9533..9632)
                     /gene="mutant version of attL"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attL2"
                     /note="recombination site for the Gateway(R) LR reaction"
     LTR             9776..10009
                     /note="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10081..10202
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      10242..10377
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(10398..10416)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10426..10442)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      10584..80
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
       61 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      301 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      361 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
      421 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
      481 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
      541 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
      601 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
      661 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
      721 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
      781 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
      841 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
      901 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
      961 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1021 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     2161 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     2221 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     2281 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     2341 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     2401 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     2461 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     2521 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     2581 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     2641 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     2701 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     2761 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     2821 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     2881 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     2941 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     3001 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     3061 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     3121 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     3181 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     3241 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     3301 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     3361 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     3421 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     3481 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     3541 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     3601 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     3661 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     3721 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     3781 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     3841 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     3901 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     3961 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     4021 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     4081 ctcgacggta tcgatcacga gactagcctc gagcggccgc aatatttgca tgtcgctatg
     4141 tgttctggga aatcaccata aacgtgaaat ccctatcagt gatagagact tataagttcc
     4201 ctatcagtga tagagacacc ggtgtgttgt aaatgagcac acaaaataca catgctaaaa
     4261 tattatattc tatgaccttt ataaaatcaa ccaaaatctt ctttttaata actttagtat
     4321 caataattag aatttttatg ttcctttttg caaactttta ataaaaatga gcaaaataaa
     4381 aaaacgctag ttttagtaac tcgcgttgtt ttcttcacct ttaataatag ctactccacc
     4441 acttgttcct aagcggtcag ctcctgcttc aatcattttt tgagcatctt caaatgttct
     4501 aactccacca gctgctttaa ctaaagcatt gtctttaaca actgacttca ttagtttaac
     4561 atcttcaaat gttgcacctg attttgaaaa tcctgttgat gttttaacaa attctaatcc
     4621 agcttcaaca gctatttcac aagctttcat gatttcttct tttgttaata aacaattttc
     4681 cataatacat ttaacaacat gtgatccagc tgcttttttt acagctttca tgtcttctaa
     4741 aactaattca taatttttgt cttttaatgc accaatattt aataccatat caatttctgt
     4801 tgcaccatct ttaattgctt cagaaacttc gaatgctttt gtagctgttg tgcatgcacc
     4861 tagaggaaaa cctacaacat ttgttattcc tacatttgtg ccttttaata attctttaca
     4921 atagcttgtt caatatgaat taacacaaac tgttgcaaaa tcaaattcaa ttgcttcatc
     4981 acataattgt ttaatttcag ctttcgtagc atcttgtttt aataatgtgt gatctatata
     5041 tttgtttagt ttcatttttt ctcctatata ttcattttta attttaattc tttaataatt
     5101 tcgtctactt taactttagc gttttgaaca gattcaccaa cacctataaa ataaattttt
     5161 agtttaggtt cagttccact tgggcgaaca gcaaatcatg acttatcttc taaataaaat
     5221 tttagtaagt cttgtcctgg catattatac attccatcga tgtagtcttc aacattaaca
     5281 actttaagtc cagcaatttg agttaagggt gttgctctca atgatttcat taatggttca
     5341 atttttaatt tcttttcttc tggtttaaaa ttcaagttta aagtgaaagt gtaatatgca
     5401 cccatttctt taaataaatc ttctaaatag tctactaatg ttttattttg ttttttataa
     5461 aatcaagcag cctctgctat taatatagaa gcttgtattc catctttatc tctagctgag
     5521 tcatcaatta catatccata actttcttca taagcaaaaa caaaatttaa tccgttatct
     5581 tcttctttag caatttctct acccattcat ttaaatccag ttaaagtttt tacaatatta
     5641 actccatatt tttcatgagc gattctatca cccaaatcac ttgttacaaa acttgaatat
     5701 agagccggat tttttggaat gctatttaag cgttttagat ttgataattt tcaatcaatt
     5761 aaaattggtc ctgtttgatt tccatctaat cttacaaaat gaccatcatg ttttattgcc
     5821 attccaaatc tgtcagcatc tgggtcattc ataataataa tatctgcatc atgtttaata
     5881 ccatattcaa gcggtatttt tcatgcagga tcaaattctg gatttggatt tacaacattt
     5941 ttaaatgttt catcttcaaa tgcatgctct tcaacctcaa taacgttata tcctgattca
     6001 cgtaatattt ttggggtaaa tttagttcct gttccattaa ctgcgctaaa aataattttt
     6061 aaatcttttt tagcttcttg ctcttttttg tagaattctc gacctcgaga caaatggcag
     6121 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     6181 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     6241 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg cggctcgagg
     6301 gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct
     6361 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc
     6421 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct
     6481 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg
     6541 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg
     6601 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc
     6661 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg
     6721 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc
     6781 gttgaccgaa tcaccgacct ctctccccag ggggatctgt gagtttgggg acccttgatt
     6841 gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg
     6901 tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt
     6961 tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg
     7021 taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt
     7081 atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat
     7141 tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa
     7201 tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca
     7261 tcctggtcat catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga
     7321 ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct
     7381 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     7441 aattgtaata cgactcacta tagggcgaat tgatatgtct agattagata aaagtaaagt
     7501 gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa
     7561 actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg
     7621 ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc acttttgccc
     7681 tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc
     7741 tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca
     7801 gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa
     7861 tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca
     7921 agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt
     7981 attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg
     8041 ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtccgcgta
     8101 cagcggctcc cgggagttct agggatctgc ccctctccct cccccccccc taacgttact
     8161 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     8221 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     8281 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     8341 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     8401 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     8461 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     8521 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     8581 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     8641 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     8701 taaggatcca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     8761 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     8821 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     8881 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     8941 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     9001 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     9061 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     9121 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     9181 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     9241 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     9301 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     9361 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     9421 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     9481 cgccttcttg acgagttctt ctgaggtacc ttttttaagg gtgggcgcgc cgacccagct
     9541 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     9601 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccg cccgccccac
     9661 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcggtacc tttaagacca
     9721 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     9781 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     9841 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     9901 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     9961 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
    10021 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
    10081 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    10141 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    10201 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
    10261 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
    10321 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    10381 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
    10441 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
    10501 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
    10561 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
    10621 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
    10681 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
    10741 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
    10801 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
    10861 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
    10921 ctcggtctat tcttttgatt tataagggat tttgccgat
//

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