| 质粒名称: | p75-RFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 24092 |
| 存储实验室: | Moses Chao |
|
载体骨架基本信息 |
|
| 载体名称: | pDSRed |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 5000 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | p75NTR |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | DSRed（插入的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | n/a |
| 3' 测序引物 | DsRed1-N |
|
文献引用方法 |
|
| 材料和方法部分: | p75-RFP was a gift from Moses Chao (Addgene plasmid # 24092 ; http://n2t.net/addgene:24092 ; RRID:Addgene_24092) |
买家导航
p75RFP载体基本信息
p75RFP载体图谱质粒图谱和多克隆位点信息
p75RFP载体简介
p75RFP载体序列
LOCUS p75-RFP 5955 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS p75-RFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5955)
TITLE Chao Plasmids
REFERENCE 2 (bases 1 to 5955)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5955
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 119..422
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 423..626
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 577..597
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 2007..2684
/codon_start=1
/product="monomeric derivative of DsRed (Campbell et al.,
2002)"
/label=mRFP1
/note="mammalian codon-optimized"
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
TGA"
primer_bind complement(2137..2155)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 2405..2424
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind 2599..2622
/label=DsRed1-C
/note="DsRed1, forward primer"
polyA_signal 2801..2922
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2838..2857)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2892..2911
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2929..3384)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3066..3087)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3278..3297
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3411..3515
/gene="bla"
/label=AmpR promoter
promoter 3517..3874
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3540..3560)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3725..3860
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3787..3806
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3909..4703
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3963..3982)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4573..4592
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4891..4910)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4935..4982
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5311..5899
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5800..5819
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
661 ggactcagat ctcgagctca agcttcgaat tcgagcgctg ccgcgggagg cgggcgatgg
721 gggcaggtgc caccggccgc gccatggacg ggccgcgcct gctgctgttg ctgcttctgg
781 gggtgtccct tggaggtgcc aaggaggcat gccccacagg cctgtacaca cacagcggtg
841 agtgctgcaa agcctgcaac ctgggcgagg gtgtggccca gccttgtgga gccaaccaga
901 ccgtgtgtga gccctgcctg gacagcgtga cgttctccga cgtggtgagc gcgaccgagc
961 cgtgcaagcc gtgcaccgag tgcgtggggc tccagagcat gtcggcgccg tgcgtggagg
1021 ccgacgacgc cgtgtgccgc tgcgcctacg gctactacca ggatgagacg actgggcgct
1081 gcgaggcgtg ccgcgtgtgc gaggcgggct cgggcctcgt gttctcctgc caggacaagc
1141 agaacaccgt gtgcgaggag tgccccgacg gcacgtattc cgacgaggcc aaccacgtgg
1201 acccgtgcct gccctgcacc gtgtgcgagg acaccgagcg ccagctccgc gagtgcacac
1261 gctgggccga cgccgagtgc gaggagatcc ctggccgttg gattacacgg tccacacccc
1321 cagagggctc ggacagcaca gcccccagca cccaggagcc tgaggcacct ccagaacaag
1381 acctcatagc cagcacggtg gcaggtgtgg tgaccacagt gatgggcagc tcccagcccg
1441 tggtgacccg aggcaccacc gacaacctca tccctgtcta ttgctccatc ctggctgctg
1501 tggttgtggg ccttgtggcc tacatagcct tcaagaggtg gaacagctgc aagcagaaca
1561 agcaaggagc caacagccgg ccagtgaacc agacgccccc accagaggga gaaaaactcc
1621 acagcgacag tggcatctcc gtggacagcc agagcctgca tgaccagcag ccccacacgc
1681 agacagcctc gggccaggcc ctcaagggtg acggaggcct ctacagcagc ctgcccccag
1741 ccaagcggga ggaggtggag aagcttctca acggctctgc gggggacacc tggcggcacc
1801 tggcgggcga gctgggctac cagcccgagc acatagactc ctttacccat gaggcctgcc
1861 ccgttcgcgc cctgcttgca agctgggcca cccaggacag cgccacactg gacgccctcc
1921 tggccgccct gcgccgcatc cagcgagccg acctcgtgga gagtctgtgc agtgagtcca
1981 ctgccacatc ccgggcccgg gatccgatgg cctcctccga ggacgtcatc aaggagttca
2041 tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
2101 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg
2161 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt ccagtacggc tccaaggcct
2221 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
2281 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
2341 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
2401 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag cggatgtacc
2461 ccgaggacgg cgccctgaag ggcgagatca agatgaggct gaagctgaag gacggcggcc
2521 actacgacgc cgaggtcaag accacctaca tggccaagaa gcccgtgcag ctgcccggcg
2581 cctacaagac cgacatcaag ctggacatca cctcccacaa cgaggactac accatcgtgg
2641 aacagtacga gcgcgccgag ggccgccact ccaccggcgc ctaaggcgac tctagatcat
2701 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
2761 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
2821 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
2881 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg
2941 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
3001 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
3061 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
3121 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
3181 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
3241 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
3301 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
3361 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
3421 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
3481 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
3541 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
3601 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
3661 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
3721 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
3781 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
3841 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
3901 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
3961 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
4021 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
4081 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
4141 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
4201 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
4261 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
4321 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
4381 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
4441 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
4501 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
4561 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
4621 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
4681 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
4741 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
4801 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
4861 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
4921 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
4981 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
5041 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
5101 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
5161 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
5221 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
5281 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
5341 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
5401 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
5461 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
5521 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
5581 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
5641 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
5701 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
5761 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
5821 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
5881 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
5941 ctggcctttt gctca
//
