| 质粒名称: | pGint |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 24217 |
| 存储实验室: | Mariano Garcia-Blanco |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-N1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 3945 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | pGint,GFP splicing reporter |
| 物种来源: | Modified from Aequorea victoria and adenovirus |
| 插入大小: | 859 bp |
| 基因突变: | Modified adenovirus in GFP |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XhoI(未破坏) |
| 3' 克隆位点: | NotI(未破坏) |
| 5' 测序引物 | commercial, from Clontech |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGint was a gift from Mariano Garcia-Blanco (Addgene plasmid # 24217 ; http://n2t.net/addgene:24217 ; RRID:Addgene_24217) |
| 参考文献部分: | A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice. Bonano VI, Oltean S, Garcia-Blanco MA. Nat Protoc. 2007 . 2(9):2166-81. 10.1038/nprot.2007.292 PubMed 17853873 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGint 4804 bp ds-DNA circular SYN 09-APR-2020
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pGint
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4804)
AUTHORS Bonano VI, Oltean S, Garcia-Blanco MA
TITLE A protocol for imaging alternative splicing regulation in vivo using
fluorescence reporters in transgenic mice.
JOURNAL Nat Protoc. 2007 . 2(9):2166-81.
PUBMED 17853873
REFERENCE 2 (bases 1 to 4804)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 9, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4804
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 619..628
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
primer_bind complement(670..691)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1056..1075)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1403..1424
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 1592..1713
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1629..1648)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1683..1702
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1720..2175)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1857..1878)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2069..2088
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2202..2306
/gene="bla"
/label=AmpR promoter
promoter 2308..2665
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2331..2351)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2516..2651
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2578..2597
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2700..3494
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2754..2773)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3364..3383
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3682..3701)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3726..3773
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4102..4690
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4591..4610
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgaggc caccatggtg agcaagggcg aggagctgtt caccggggtg
661 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
721 gagggcgagg tgagtatgga tccctctaaa agcgggcatg acttctagag tagtccaggg
781 tttccgaggg tttccgtcga ctcagctcgt ctcgagggcg tactaactgg gccctttccc
841 ttttttttcc tcagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
901 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
961 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
1021 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
1081 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
1141 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
1201 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
1261 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
1321 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
1381 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
1441 ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca
1501 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
1561 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
1621 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
1681 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
1741 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
1801 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
1861 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
1921 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
1981 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
2041 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
2101 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
2161 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
2221 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
2281 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
2341 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
2401 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
2461 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
2521 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
2581 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
2641 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
2701 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
2761 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
2821 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
2881 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
2941 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
3001 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
3061 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
3121 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
3181 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
3241 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
3301 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
3361 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
3421 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
3481 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
3541 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
3601 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
3661 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
3721 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
3781 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
3841 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
3901 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
3961 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
4021 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
4081 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
4141 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
4201 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
4261 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
4321 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
4381 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
4441 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
4501 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
4561 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
4621 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
4681 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
4741 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
4801 gcat
//
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