| 质粒名称: | pEGFP-C1-NuMA |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 81029 |
| 存储实验室: | Michael Mancini |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 |
| 载体出品公司: | Clontech |
| 空载体大小: | 4731bp |
| 完整质粒大小: | 11654bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | Kanamycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 6308 bp |
| GenBank编号 | NM_006185 |
| 启动子: | CMV |
| 标签/融合蛋白: | EGFP (N terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BglII (not destroyed) |
| 3’克隆位点: | BamHI (not destroyed) |
| 5’测序引物 | EGFP-C |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pEGFP-C1-NuMA was a gift from Michael Mancini (Addgene plasmid # 81029 ; http://n2t.net/addgene:81029 ; RRID:Addgene_81029) |
买家导航
pEGFP-C1-NuMA载体质粒基本信息
pEGFP-C1-NuMA质粒图谱载体图谱和pEGFP-C1-NuMA载体序列质粒序列多克隆位点信息
pEGFP-C1-NuMA质粒载体简介
pEGFP-C1-NuMA质粒序列载体序列
LOCUS pEGFP-C1-NuMA 11654 bp DNA circular SYN 25-AUG-2024 DEFINITION Expresses human NuMA with N-terminal EGFP tag in mammalian cells. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11654) TITLE Mancini Lab Plasmids REFERENCE 2 (bases 1 to 11654) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3 https://www.biofeng.com COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..11654 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 607..616 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" CDS 613..1329 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 613..1329 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(658..679) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(919..938) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1266..1287 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 8442..8563 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8479..8498) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8533..8552 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(8570..9025) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8707..8728) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 8919..8938 /label=F1ori-R /note="F1 origin, reverse primer" promoter 9052..9156 /gene="bla" /label=AmpR promoter promoter 9158..9515 /label=SV40 promoter /note="SV40 enhancer and early promoter" enhancer 9158..9349 /label=SV40 enhancer /note="enhancer for the SV40 early promoter (Herr, 1993)" primer_bind complement(9181..9201) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 9366..9501 /label=SV40 ori /note="SV40 origin of replication" CDS 9550..10344 /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" CDS 9550..10344 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(9604..9623) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 10214..10233 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(10532..10551) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 10576..10623 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 10952..11540 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11441..11460 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggactcag atctatgaca ctccacgcca cccggggggc tgcactcctc 1381 tcttgggtga acagtctaca cgtggctgac cctgtggagg ctgtgctgca gctccaggac 1441 tgcagcatct tcatcaagat cattgacaga atccatggca ctgaagaggg acagcaaatc 1501 ttgaagcagc cggtgtcaga gagactggac tttgtgtgca gttttctgca gaaaaatcga 1561 aaacatccct cttccccaga atgcctggta tctgcacaga aggtgctaga gggatcagag 1621 ctggaactgg cgaagatgac catgctgctc ttataccact ctaccatgag ctccaaaagt 1681 cccagggact gggaacagtt tgaatataaa attcaggctg agttggctgt cattcttaaa 1741 tttgtgctgg accatgagga cgggctaaac cttaatgagg acctagagaa cttcctacag 1801 aaagctcctg tgccttctac ctgttctagc acattccctg aagagctctc cccacctagc 1861 caccaggcca agagggagat tcgcttccta gagctacaga aggttgcctc ctcttccagt 1921 gggaacaact ttctctcagg ttctccagct tctcccatgg gtgatatcct gcagacccca 1981 cagttccaga tgagacggct gaagaagcag cttgctgatg agagaagtaa tagggatgag 2041 ctggagctgg agctagctga gaaccgcaag ctcctcaccg agaaggatgc acagatagcc 2101 atgatgcagc agcgcattga ccgcctagcc ctgctgaatg agaagcaggc ggccagccca 2161 ctggagccca aggagcttga ggagctgcgt gacaagaatg agagccttac catgcggctg 2221 catgaaaccc tgaagcagtg ccaggacctg aagacagaga agagccagat ggatcgcaaa 2281 atcaaccagc tttcggagga gaatggagac ctttccttta agctgcggga gtttgccagt 2341 catctgcagc agctacagga tgccctcaat gagctgacgg aggagcacag caaggccact 2401 caggagtggc tagagaagca ggcccagctg gagaaggagc tcagcgcagc cctgcaggac 2461 aagaaatgcc ttgaagagaa gaacgaaatc cttcagggaa aactttcaca gctggaagaa 2521 cacttgtccc agctgcagga taacccaccc caggagaagg gcgaggtgct gggtgatgtc 2581 ttgcagctgg aaaccttgaa gcaagaggca gccactcttg ctgcaaacaa cacacagctc 2641 caagccaggg tagagatgct ggagactgag cgaggccagc aggaagccaa gctgcttgct 2701 gagcggggcc acttcgaaga agaaaagcag cagctgtcta gcctgatcac tgacctgcag 2761 agctccatct ccaacctcag ccaggccaag gaagagctgg agcaggcctc ccaggctcat 2821 ggggcccggt tgactgccca ggtggcctct ctgacctctg agctcaccac actcaatgcc 2881 accatccagc aacaggatca agaactggct ggcctgaagc agcaggccaa agagaagcag 2941 gcccagctag cacagaccct ccaacagcaa gaacaggcct cccagggcct ccgccaccag 3001 gtggagcagc taagcagtag cctgaagcag aaggagcagc agttgaagga ggtagcggag 3061 aagcaggagg caactaggca ggaccatgcc cagcaactgg ccactgctgc agaggagcga 3121 gaggcctcct taagggagcg ggatgcggct ctcaagcagc tggaggcact ggagaaggag 3181 aaggctgcca agctggagat tctgcagcag caacttcagg tggctaatga agcccgggac 3241 agtgcccaga cctcagtgac acaggcccag cgggagaagg cagagctgag ccggaaggtg 3301 gaggaactcc aggcctgtgt tgagacagcc cgccaggaac agcatgaggc ccaggcccag 3361 gttgcagagc tagagttgca gctgcggtct gagcagcaaa aagcaactga gaaagaaagg 3421 gtggcccagg agaaggacca gctccaggag cagctccagg ccctcaaaga gtccttgaag 3481 gtcaccaagg gcagccttga agaggagaag cgcagggctg cagatgccct ggaagagcag 3541 cagcgttgta tctctgagct gaaggcagag acccgaagcc tggtggagca gcataagcgg 3601 gaacgaaagg agctggaaga agagagggct gggcgcaagg ggctggaggc tcgattacag 3661 cagcttgggg aggcccatca ggctgagact gaagtcctgc ggcgggagct ggcagaggcc 3721 atggctgccc agcacacagc tgagagtgag tgtgagcagc tcgtcaaaga agtagctgcc 3781 tggcgtgagc ggtatgagga tagccagcaa gaggaggcac agtatggcgc catgttccag 3841 gaacagctga tgactttgaa ggaggaatgt gagaaggccc gccaggagct gcaggaggca 3901 aaggagaagg tggcaggcat agaatcccac agcgagctcc agataagccg gcagcagaac 3961 aaactagctg agctccatgc caacctggcc agagcactcc agcaggtcca agagaaggaa 4021 gtcagggccc agaagcttgc agatgacctc tccactctgc aggaaaagat ggctgccacc 4081 agcaaagagg tggcccgctt ggagaccttg gtgcgcaagg caggtgagca gcaggaaaca 4141 gcctcccggg agttagtcaa ggagcctgcg agggcaggag acagacagcc cgagtggctg 4201 gaagagcaac agggacgcca gttctgcagc acacaggcag cgctgcaggc tatggagcgg 4261 gaggcagagc agatgggcaa tgagctggaa cggctgcggg ccgcgctgat ggagagccag 4321 gggcagcagc aggaggagcg tgggcagcag gaaagggagg tggcgcggct gacccaggag 4381 cggggccgtg cccaggctga ccttgccctg gagaaggcgg ccagagcaga gcttgagatg 4441 cggctgcaga acgccctcaa cgagcagcgt gtggagttcg ctaccctgca agaggcactg 4501 gctcatgccc tgacggaaaa ggaaggcaag gaccaggagt tggccaagct tcgtggtctg 4561 gaggcagccc agataaaaga gctggaggaa cttcggcaaa ccgtgaagca actgaaggaa 4621 cagctggcta agaaagaaaa ggagcacgca tctggctcag gagcccaatc tgaggctgct 4681 ggcaggacag agccaacagg ccccaagctg gaagcactgc gggcagaggt gagcaagctg 4741 gaacagcaat gccagaagca gcaggagcag gctgacagcc tggaacgcag cctcgaggct 4801 gagcgggcct cccgggctga gcgggacagt gctctggaga ctctgcaggg ccagttagag 4861 gagaaggccc aggagctagg gcacagtcag agtgccttag cctcggccca acgggagttg 4921 gctgccttcc gcaccaaggt acaagaccac agcaaggctg aagatgagtg gaaggcccag 4981 gtggcccggg gccggcaaga ggctgagagg aaaaatagcc tcatcagcag cttggaggag 5041 gaggtgtcca tcctgaatcg ccaggtcctg gagaaggagg gggagagcaa ggagttgaag 5101 cggctggtga tggccgagtc agagaagagc cagaagctgg aggagaggct gcgcctgctg 5161 caggcagaga cagccagcaa cagtgccaga gctgcagaac gcagctctgc tctgcgggag 5221 gaggtgcaga gcctccggga ggaggctgag aaacagcggg tggcttcaga gaacctgcgg 5281 caggagctga cctcacaggc tgagcgtgcg gaggagctgg gccaagaatt gaaggcgtgg 5341 caggagaagt tcttccagaa agagcaggcc ctctccaccc tgcagctcga gcacaccagc 5401 acacaggccc tggtgagtga gctgctgcca gctaagcacc tctgccagca gctgcaggcc 5461 gagcaggccg ctgccgagaa acgccaccgt gaggagctgg agcagagcaa gcaggccgct 5521 gggggactgc gggcagagct gctgcgggcc cagcgggagc ttggggagct gattcctctg 5581 cggcagaagg tggcagagca ggagcgaaca gctcagcagc tgcgggcaga gaaggccagc 5641 tatgcagagc agctgagcat gctgaagaag gcgcatggcc tgctggcaga ggagaaccgg 5701 gggctgggtg agcgggccaa ccttggccgg cagtttctgg aagtggagtt ggaccaggcc 5761 cgggaaaagt atgtccaaga gttggcagcc gtacgtgctg atgctgagac ccgtctggct 5821 gaggtgcagc gagaagcaca gagcactgcc cgggagctgg aggtgatgac tgccaagtat 5881 gagggtgcca aggtcaaggt cctggaggag aggcagcggt tccaggaaga gaggcagaaa 5941 ctcactgccc aggtggaaga actgagtaag aaactggctg actctgacca agccagcaag 6001 gtgcagcagc agaagctgaa ggctgtccag gctcagggag gcgagagcca gcaggaggcc 6061 cagcgcttcc aggcccagct gaatgaactg caagcccagt tgagccagaa ggagcaggca 6121 gctgagcact ataagctgca gatggagaaa gccaaaacac attatgatgc caagaagcag 6181 cagaaccaag agctgcagga gcagctgcgg agcctggagc agctgcagaa ggaaaacaaa 6241 gagctgcgag ctgaagctga acggctgggc catgagctac agcaggctgg gctgaagacc 6301 aaggaggctg aacagacctg ccgccacctt actgcccagg tgcgcagcct ggaggcacag 6361 gttgcccatg cagaccagca gcttcgagac ctgggcaaat tccaggtggc aactgatgct 6421 ttaaagagcc gtgagcccca ggctaagccc cagctggact tgagtattga cagcctggat 6481 ctgagctgcg aggaggggac cccactcagt atcaccagca agctgcctcg tacccagcca 6541 gacggcacca gcgtccctgg agaaccagcc tcacctatct cccagcgcct gccccccaag 6601 gtagaatccc tggagagtct ctacttcact cccatccctg ctcggagtca ggcccccctg 6661 gagagcagcc tggactccct gggagacgtc ttcctggact cgggtcgtaa gacccgctcc 6721 gctcgtcggc gcaccacgca gatcatcaac atcaccatga ccaagaagct agatgtggaa 6781 gagccagaca gcgccaactc atcgttctac agcacgcggt ctgctcctgc ttcccaggct 6841 agcctgcgag ccacctcctc tactcagtct ctagctcgcc tgggttctcc cgattatggc 6901 aactcagccc tgctcagctt gcctggctac cgccccacca ctcgcagttc tgctcgtcgt 6961 tcccaggccg gggtgtccag tggggcccct ccaggaagga acagcttcta catgggcact 7021 tgccaggatg agcctgagca gctggatgac tggaaccgca ttgcagagct gcagcagcgc 7081 aatcgagtgt gccccccaca tctgaagacc tgctatcccc tggagtccag gccttccctg 7141 agcctgggca ccatcacaga tgaggagatg aaaactggag acccccaaga gaccctgcgc 7201 cgagccagca tgcagccaat ccagatagcc gagggcactg gcatcaccac ccggcagcag 7261 cgcaaacggg tctccctaga gccccaccag ggccctggaa ctcctgagtc taagaaggcc 7321 accagctgtt tcccacgccc catgactccc cgagaccgac atgaagggcg caaacagagc 7381 actactgagg cccagaagaa agcagctcca gcttctacta aacaggctga ccggcgccag 7441 tcgatggcct tcagcatcct caacacaccc aagaagctag ggaacagcct tctgcggcgg 7501 ggagcctcaa agaaggccct gtccaaggct tcccccaaca ctcgcagtgg aacccgccgt 7561 tctccgcgca ttgccaccac cacagccagt gccgccactg ctgccgccat tggtgccacc 7621 cctcgagcca agggcaaggc aaagcactaa agggccagta ccagtgagtg gccccacctg 7681 tgtccccgat gctgccgtca cctggtcctc cgcctactgt ccctctcagt gccttctctc 7741 agctcccagg ccaacagtag ccaaacccct agagacagtg atgcctgccc gcaccctggc 7801 ctggcccctg gtccttcact ggcgccttct cggagctggc ccagggggcc tggagcatgg 7861 acagtgtggg cgctctccct accttgcctc cttttttctt aaagcaaagt cacttctcca 7921 tcacaaccag atttgaggct ggttttgatg gctgggtcct tgggcctggc cagtcttcct 7981 cttagcctct ggatctagaa gggaccataa gaggagtagg ccctggttcc tgctgtcctg 8041 gtggctgggc ccagcagggg ccctcactct tgaagtccag gactgggtct gacctggtgg 8101 gagcacctgc cagaggatgc tctttcccag gacggatggg ccctgtgtct caggagtggg 8161 gttgggggac agccttcagc agcagctcac accctacctt ccccagactt gcactggggt 8221 gggatttgga gtgatgggaa ggtttttaag ggccggggat ggatcttttc taaatgttat 8281 tacttgtaaa taaagtctat ttttcggaat tcggatccac cggatctaga taactgatca 8341 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc 8401 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt 8461 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 8521 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta aattgtaagc 8581 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa 8641 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt 8701 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg 8761 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt 8821 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga 8881 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 8941 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg 9001 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 9061 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 9121 tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt 9181 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 9241 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 9301 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 9361 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 9421 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 9481 gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga 9541 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag 9601 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc 9661 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg 9721 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc 9781 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg 9841 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct 9901 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg 9961 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat 10021 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc 10081 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg 10141 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc 10201 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct 10261 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat 10321 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga 10381 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct 10441 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg 10501 agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag 10561 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg 10621 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc 10681 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg 10741 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact 10801 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 10861 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 10921 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 10981 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 11041 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 11101 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 11161 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 11221 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 11281 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 11341 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 11401 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 11461 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 11521 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 11581 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 11641 ttaccgccat gcat //
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