| 质粒名称: | pCXLE-hSK |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 27078 |
| 存储实验室: | Shinya Yamanaka |
|
载体骨架基本信息 |
|
| 载体名称: | pCXLE |
| 载体抗性: | 氨苄西林 |
| 空载体大小: | 10180 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
| 宿生菌： | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SOX2, KLF4 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2513 bp |
| 克隆方法: | 限制性酶 |
| 5' 克隆位点: | Ecori（未销毁） |
| 3' 克隆位点: | Ecori（未销毁） |
| 5' 测序引物 | pCAG-F |
| 3' 测序引物 | WPRE-R |
|
文献引用方法 |
|
| 材料和方法部分: | pCXLE-hSK was a gift from Shinya Yamanaka (Addgene plasmid # 27078 ; http://n2t.net/addgene:27078 ; RRID:Addgene_27078) |
| 参考文献部分: | A more efficient method to generate integration-free human iPS cells. Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S. Nat Methods. 2011 Apr 3. ():. 10.1038/nmeth.1591 PubMed 21460823 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCXLE-hSK 12693 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Integration-free (episomal) expression of human SOX2 and KLF4.
ACCESSION .
VERSION .
KEYWORDS pCXLE-hSK
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12693)
AUTHORS Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong
H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
M, Takahashi J, Saji H, Yamanaka S
TITLE A more efficient method to generate integration-free human iPS
cells.
JOURNAL Nat Methods. 2011 Apr 3. ():.
PUBMED 21460823
REFERENCE 2 (bases 1 to 12693)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12693
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 4..383
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 385..661
/label=chicken beta-actin promoter
intron 662..1678
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1602..1624
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1686..1705
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS 2718..2783
/codon_start=1
/product="2A peptide from foot-and-mouth disease virus
polyprotein"
/label=F2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="VKQTLNFDLLKLAGDVESNPGP"
misc_feature 4271..4859
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4324..4344)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4742..4753)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4949..4968)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 5014..5069
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(5068..5087)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(5430..5446)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5430..5446)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5443..5465)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5454..5470
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5478..5508)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5523..5544
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
protein_bind complement(5633..5666)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS 6127..8052
/codon_start=1
/product="Epstein-Barr nuclear antigen 1, also known as
EBNA-1"
/label=EBNA1
/note="crucial for latent viral infection, and for episomal
amplification of vectors containing the oriP origin (Okita
et al., 2013)"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
GEEGQE"
protein_bind complement(8220..8253)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
rep_origin 8440..10229
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
primer_bind complement(10771..10788)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10942..11530)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(11022..11041)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(11701..12561)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12324..12343
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12562..12666)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
541 ggggcgggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgcccttca
1741 ccatgtacaa catgatggag acggagctga agccgccggg cccgcagcaa acttcggggg
1801 gcggcggcgg caactccacc gcggcggcgg ccggcggcaa ccagaaaaac agcccggacc
1861 gcgtcaagcg gcccatgaat gccttcatgg tgtggtcccg cgggcagcgg cgcaagatgg
1921 cccaggagaa ccccaagatg cacaactcgg agatcagcaa gcgcctgggc gccgagtgga
1981 aacttttgtc ggagacggag aagcggccgt tcatcgacga ggctaagcgg ctgcgagcgc
2041 tgcacatgaa ggagcacccg gattataaat accggccccg gcggaaaacc aagacgctca
2101 tgaagaagga taagtacacg ctgcccggcg ggctgctggc ccccggcggc aatagcatgg
2161 cgagcggggt cggggtgggc gccggcctgg gcgcgggcgt gaaccagcgc atggacagtt
2221 acgcgcacat gaacggctgg agcaacggca gctacagcat gatgcaggac cagctgggct
2281 acccgcagca cccgggcctc aatgcgcacg gcgcagcgca gatgcagccc atgcaccgct
2341 acgacgtgag cgccctgcag tacaactcca tgaccagctc gcagacctac atgaacggct
2401 cgcccaccta cagcatgtcc tactcgcagc agggcacccc tggcatggct cttggctcca
2461 tgggttcggt ggtcaagtcc gaggccagct ccagcccccc tgtggttacc tcttcctccc
2521 actccagggc gccctgccag gccggggacc tccgggacat gatcagcatg tatctccccg
2581 gcgccgaggt gccggaaccc gccgccccca gcagacttca catgtcccag cactaccaga
2641 gcggcccggt gcccggcacg gccattaacg gcacactgcc cctctcacac atgggggatc
2701 tcaaaattgt cgctcctgtc aaacaaactc ttaactttga tttactcaaa ctggctgggg
2761 atgtagaaag caatccaggt ccactcatga acaattcgcc cttcaccatg gctgtcagtg
2821 acgcgctgct cccatctttc tccacgttcg cgtctggccc ggcgggaagg gagaagacac
2881 tgcgtcaagc aggtgccccg aataaccgct ggcgggagga gctctcccac atgaagcgac
2941 ttcccccagt gcttcccggc cgcccctatg acctggcggc ggcgaccgtg gccacagacc
3001 tggagagcgg cggagccggt gcggcttgcg gcggtagcaa cctggcgccc ctacctcgga
3061 gagagaccga ggagttcaac gatctcctgg acctggactt tattctctcc aattcgctga
3121 cccatcctcc ggagtcagtg gccgccaccg tgtcctcgtc agcgtcagcc tcctcttcgt
3181 cgtcgccgtc gagcagcggc cctgccagcg cgccctccac ctgcagcttc acctatccga
3241 tccgggccgg gaacgacccg ggcgtggcgc cgggcggcac gggcggaggc ctcctctatg
3301 gcagggagtc cgctccccct ccgacggctc ccttcaacct ggcggacatc aacgacgtga
3361 gcccctcggg cggcttcgtg gccgagctcc tgcggccaga attggacccg gtgtacattc
3421 cgccgcagca gccgcagccg ccaggtggcg ggctgatggg caagttcgtg ctgaaggcgt
3481 cgctgagcgc ccctggcagc gagtacggca gcccgtcggt catcagcgtc agcaaaggca
3541 gccctgacgg cagccacccg gtggtggtgg cgccctacaa cggcgggccg ccgcgcacgt
3601 gccccaagat caagcaggag gcggtctctt cgtgcaccca cttgggcgct ggaccccctc
3661 tcagcaatgg ccaccggccg gctgcacacg acttccccct ggggcggcag ctccccagca
3721 ggactacccc gaccctgggt cttgaggaag tgctgagcag cagggactgt caccctgccc
3781 tgccgcttcc tcccggcttc catccccacc cggggcccaa ttacccatcc ttcctgcccg
3841 atcagatgca gccgcaagtc ccgccgctcc attaccaaga gctcatgcca cccggttcct
3901 gcatgccaga ggagcccaag ccaaagaggg gaagacgatc gtggccccgg aaaaggaccg
3961 ccacccacac ttgtgattac gcgggctgcg gcaaaaccta cacaaagagt tcccatctca
4021 aggcacacct gcgaacccac acaggtgaga aaccttacca ctgtgactgg gacggctgtg
4081 gatggaaatt cgcccgctca gatgaactga ccaggcacta ccgtaaacac acggggcacc
4141 gcccgttcca gtgccaaaaa tgcgaccgag cattttccag gtcggaccac ctcgccttac
4201 acatgaagag acatttttaa aagggcgaat tgcctgcagg aattcaagct tcggggacta
4261 gtcatatgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
4321 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
4381 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
4441 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
4501 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
4561 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
4621 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
4681 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
4741 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
4801 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
4861 tcggtaaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca
4921 atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca
4981 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
5041 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
5101 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
5161 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
5221 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
5281 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
5341 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
5401 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
5461 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
5521 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
5581 aacctgtcgt gccagcggat ctcaattccg atcatattca ataaccctta atataacttc
5641 gtataatgta tgctatacga agttattagg tctgaagagg agtttacgtc cagccaagct
5701 taggatcaat tctcatgttt gacagcttat catcgataag ctgatcctca caggccgcac
5761 ccagcttttc ttccgttgcc ccagtagcat ctctgtctgg tgaccttgaa gaggaagagg
5821 aggggtcccg agaatcccca tccctaccgt ccagcaaaaa gggggacgag gaatttgagg
5881 cctggcttga ggctcaggac gcaaatcttg aggatgttca gcgggagttt tccgggctgc
5941 gagtaattgg tgatgaggac gaggatggtt cggaggatgg ggaattttca gacctggatc
6001 tgtctgacag cgaccatgaa ggggatgagg gtgggggggc tgttggaggg ggcaggagtc
6061 tgcactccct gtattcactg agcgtcgtct aataaagatg tctattgatc tcttttagtg
6121 tgaatcatgt ctgacgaggg gccaggtaca ggacctggaa atggcctagg agagaaggga
6181 gacacatctg gaccagaagg ctccggcggc agtggacctc aaagaagagg gggtgataac
6241 catggacgag gacggggaag aggacgagga cgaggaggcg gaagaccagg agccccgggc
6301 ggctcaggat cagggccaag acatagagat ggtgtccgga gaccccaaaa acgtccaagt
6361 tgcattggct gcaaagggac ccacggtgga acaggagcag gagcaggagc gggaggggca
6421 ggagcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc aggaggggca
6481 ggaggggcag gaggggcagg agcaggagga ggggcaggag caggaggagg ggcaggaggg
6541 gcaggagggg caggagcagg aggaggggca ggagcaggag gaggggcagg aggggcagga
6601 gcaggaggag gggcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga
6661 ggaggggcag gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggagca
6721 ggaggagggg caggagcagg aggggcagga ggggcaggag gggcaggagc aggaggggca
6781 ggagcaggag gaggggcagg aggggcagga ggggcaggag caggaggggc aggagcagga
6841 ggggcaggag caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca
6901 ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggaggggc aggaggggca
6961 ggagcaggag gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga
7021 ggggcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg aggggcagga
7081 gcaggagggg caggagcagg aggtggaggc cggggtcgag gaggcagtgg aggccggggt
7141 cgaggaggta gtggaggccg gggtcgagga ggtagtggag gccgccgggg tagaggacgt
7201 gaaagagcca gggggggaag tcgtgaaaga gccaggggga gaggtcgtgg acgtggagaa
7261 aagaggccca ggagtcccag tagtcagtca tcatcatccg ggtctccacc gcgcaggccc
7321 cctccaggta gaaggccatt tttccaccct gtaggggaag ccgattattt tgaataccac
7381 caagaaggtg gcccagatgg tgagcctgac gtgcccccgg gagcgataga gcagggcccc
7441 gcagatgacc caggagaagg cccaagcact ggaccccggg gtcagggtga tggaggcagg
7501 cgcaaaaaag gagggtggtt tggaaagcat cgtggtcaag gaggttccaa cccgaaattt
7561 gagaacattg cagaaggttt aagagctctc ctggctagga gtcacgtaga aaggactacc
7621 gacgaaggaa cttgggtcgc cggtgtgttc gtatatggag gtagtaagac ctccctttac
7681 aacctaaggc gaggaactgc ccttgctatt ccacaatgtc gtcttacacc attgagtcgt
7741 ctcccctttg gaatggcccc tggacccggc ccacaacctg gcccgctaag ggagtccatt
7801 gtctgttatt tcatggtctt tttacaaact catatatttg ctgaggtttt gaaggatgcg
7861 attaaggacc ttgttatgac aaagcccgct cctacctgca atatcagggt gactgtgtgc
7921 agctttgacg atggagtaga tttgcctccc tggtttccac ctatggtgga aggggctgcc
7981 gcggagggtg atgacggaga tgacggagat gaaggaggtg atggagatga gggtgaggaa
8041 gggcaggagt gatgtaactt gttaggagac gccctcaatc gtattaaaag ccgtgtattc
8101 ccccgcacta aagaataaat ccccagtaga catcatgcgt gctgttggtg tatttctggc
8161 catctgtctt gtcaccattt tcgtcctccc aacatggggc aattgccgga acccttaata
8221 taacttcgta taatgtatgc tatacgaagt tattaggtcc ctcgaagagg ttcactagcg
8281 gatctcaatt gggcataccc atgttgtcac gtcactcagc tccgcgctca acaccttctc
8341 gcgttggaaa acattagcga catttacctg gtgagcaatc agacatgcga cggctttagc
8401 ctggcctcct taaattcacc taagaatggg agcaaccagc aggaaaagga caagcagcga
8461 aaattcacgc ccccttggga ggtggcggca tatgcaaagg atagcactcc cactctacta
8521 ctgggtatca tatgctgact gtatatgcat gaggatagca tatgctaccc ggatacagat
8581 taggatagca tatactaccc agatatagat taggatagca tatgctaccc agatatagat
8641 taggatagcc tatgctaccc agatataaat taggatagca tatactaccc agatatagat
8701 taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc agatatagat
8761 taggatagca tatgctaccc agatatagat taggatagca tatgctatcc agatatttgg
8821 gtagtatatg ctacccagat ataaattagg atagcatata ctaccctaat ctctattagg
8881 atagcatatg ctacccggat acagattagg atagcatata ctacccagat atagattagg
8941 atagcatatg ctacccagat atagattagg atagcctatg ctacccagat ataaattagg
9001 atagcatata ctacccagat atagattagg atagcatatg ctacccagat atagattagg
9061 atagcctatg ctacccagat atagattagg atagcatatg ctatccagat atttgggtag
9121 tatatgctac ccatggcaac attagcccac cgtgctctca gcgacctcgt gaatatgagg
9181 accaacaacc ctgtgcttgg cgctcaggcg caagtgtgtg taatttgtcc tccagatcgc
9241 agcaatcgcg cccctatctt ggcccgccca cctacttatg caggtattcc ccggggtgcc
9301 attagtggtt ttgtgggcaa gtggtttgac cgcagtggtt agcggggtta caatcagcca
9361 agttattaca cccttatttt acagtccaaa accgcagggc ggcgtgtggg ggctgacgcg
9421 tgcccccact ccacaatttc aaaaaaaaga gtggccactt gtctttgttt atgggcccca
9481 ttggcgtgga gccccgttta attttcgggg gtgttagaga caaccagtgg agtccgctgc
9541 tgtcggcgtc cactctcttt ccccttgtta caaatagagt gtaacaacat ggttcacctg
9601 tcttggtccc tgcctgggac acatcttaat aaccccagta tcatattgca ctaggattat
9661 gtgttgccca tagccataaa ttcgtgtgag atggacatcc agtctttacg gcttgtcccc
9721 accccatgga tttctattgt taaagatatt cagaatgttt cattcctaca ctagtattta
9781 ttgcccaagg ggtttgtgag ggttatattg gtgtcatagc acaatgccac cactgaaccc
9841 cccgtccaaa ttttattctg ggggcgtcac ctgaaacctt gttttcgagc acctcacata
9901 caccttactg ttcacaactc agcagttatt ctattagcta aacgaaggag aatgaagaag
9961 caggcgaaga ttcaggagag ttcactgccc gctccttgat cttcagccac tgcccttgtg
10021 actaaaatgg ttcactaccc tcgtggaatc ctgaccccat gtaaataaaa ccgtgacagc
10081 tcatggggtg ggagatatcg ctgttcctta ggaccctttt actaacccta attcgatagc
10141 atatgcttcc cgttgggtaa catatgctat tgaattaggg ttagtctgga tagtatatac
10201 tactacccgg gaagcatatg ctacccgttt agggttaaca agggggcctt ataaacacta
10261 ttgctaatgc cctcttgagg gtccgcttat cggtagctac acaggcccct ctgattgacg
10321 ttggtgtagc ctcccgtagt cttcctgggc ccctgggagg tacatgtccc ccagcattgg
10381 tgtaagagct tcagccaaga gttacacata aaggcaatgt tgtgttgcag tccacagact
10441 gcaaagtctg ctccaggatg aaagccactc agtgttggca aatgtgcaca tccatttata
10501 aggatgtcaa ctacagtcag agaacccctt tgtgtttggt ccccccccgt gtcacatgtg
10561 gaacagggcc cagttggcaa gttgtaccaa ccaactgaag ggattacatg cactgccccg
10621 cgaagaaggg gcagagatgt cgtagtcagg tttagttcgt ccggggcggg gcatcgatcc
10681 tctagagtcg acgctagcgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg
10741 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
10801 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
10861 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
10921 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
10981 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
11041 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
11101 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
11161 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
11221 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
11281 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
11341 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
11401 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
11461 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
11521 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
11581 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
11641 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
11701 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
11761 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
11821 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
11881 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
11941 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
12001 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
12061 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
12121 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
12181 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
12241 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
12301 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
12361 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
12421 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
12481 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
12541 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
12601 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
12661 tccgcgcaca tttccccgaa aagtgccacc tgg
//
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