| 质粒名称: | pLM2_42_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 154238 |
| 存储实验室: | Arnold Driessen |
|
载体骨架基本信息 |
|
| 载体名称: | pAGM4673 |
| 载体出品公司: | MoClo Tool Kit of Sylvestre Marillonnet |
| 空载体大小: | 8112 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达，合成生物学；真菌表达 |
| 筛选标记: | amdS fungal selection |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | pgndA:QfDBD-VP16AD-GFP-NLS,P(various):DsRed-SKL,pGpdA:amdS |
| 物种来源: | 合成的真菌表达，红曲霉（原名产黄曲霉） |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | pGndA (An11g02040 A. niger) |
| 标签/融合蛋白: | GFP-NLS；DsRed SKL（主干上的C终端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLM2_42_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS was a gift from Arnold Driessen (Addgene plasmid # 154238 ; http://n2t.net/addgene:154238 ; RRID:Addgene_154238) |
| 参考文献部分: | Synthetic control devices for gene regulation in Penicillium chrysogenum. Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y. Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 10.1186/s12934-019-1253-3. 10.1186/s12934-019-1253-3 PubMed 31739777 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLM2_42_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS 13682 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor,
activating DsRed-SKL with promoter 5xQUAS+CP_pcbC.
ACCESSION .
VERSION .
KEYWORDS pLM2_42_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13682)
AUTHORS Mozsik L, Buttel Z, Bovenberg RAL, Driessen AJM, Nygard Y
TITLE Synthetic control devices for gene regulation in Penicillium
chrysogenum.
JOURNAL Microb Cell Fact. 2019 Nov 18;18(1):203. doi:
10.1186/s12934-019-1253-3.
PUBMED 31739777
REFERENCE 2 (bases 1 to 13682)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13682
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 2222..2332
/codon_start=1
/product="3 tandem repeats of the minimal activation domain
of herpes simplex virus VP16 (Cheng et al., 2013)"
/label=VP48
/translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
CDS 2345..3061
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 2345..3061
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2390..2411)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2651..2670)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2998..3019
/label=EGFP-C
/note="EGFP, forward primer"
CDS 3062..3082
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
intron 4254..4369
/gene="gpdA"
/label=gpdA intron
/note="intron from the Aspergillus nidulans
glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
1990)"
CDS 6952..7626
/codon_start=1
/product="rapidly maturing tetrameric variant of DsRed
fluorescent protein (Bevis and Glick, 2002)"
/label=DsRed-Express
/note="mammalian codon-optimized"
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
LFL"
primer_bind complement(7082..7100)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind complement(7132..7152)
/label=DsRed1-N
/note="DsRed1, reverse primer"
primer_bind 7544..7567
/label=DsRed1-C
/note="DsRed1, forward primer"
misc_feature 9460..9484
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin 9572..10282
/label=oriV
/note="incP origin of replication"
promoter 10303..10407
/gene="bla"
/label=AmpR promoter
CDS 10408..11202
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
rep_origin 11373..11961
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 11862..11881
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS 12297..13445
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/label=trfA
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
misc_feature 13591..13615
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
ORIGIN
1 gtgccgaatt cggatccgga gcctgcagga tgggcctcca caaccctgcc ctaacattgt
61 tcgtagaaga tctgccagca ggcttatgtc caggcccctc tcaacccgcc ctttcctttc
121 agctcgggcg ccgtacttgc cccacccagg cgtgttccgc tgaactgccc atacccaggc
181 cagcggactg ataaccattt ctctttatgc tttgctattg ggcttgtgac tcgttcttgg
241 aggcaaggtt atcagattga atagaagatt cggtgatgca gcaaatagcg actgttcgtt
301 gcggggtccg aacccgctcg gcagcaccgg gctctcccta ctatccctcg atagcagtgg
361 cgtgcgcggg ctgtcgaccc tgtccatcct gaaagagttg atattgaagg tagtaggtac
421 ggtgcagggt aacgcgtgat atcctgtcct cagtcttaag acttctcacc tatatcaccc
481 gcacttaccc tagagtgccg cttaggtgct aagggcacat tgagtattgg ccgtgtagaa
541 tatatagctt aagtacggcc aagcagacgg gaagccctgt tctccacacc ctatggtcgt
601 atatatcagg cttctaccgg gaaacgatta agagtgtata atggactgaa aatcaatatg
661 aacgggacaa tgctcaagtt aaattagtta ggcatcctaa tctctactaa atgttctatc
721 tagagatcgg ggtactatag gcccgtacgt taatcactct acgcttctct cccttaggta
781 tagtgtaggt aggggctaga catttatatg agtcagatgg tacaaacggt agcgctgcaa
841 gaattcaagc ttggagtctt gcgttacggg cgtattttgc tgcggccggt ggtgcccctc
901 catgccccgc catctttcaa agctcctggc gacgccgtca tctccgaaca ttctcccccc
961 aaaggaatca attggcaatt ggagtctagt aaagtggtgt ttgtcatcag taaggagttg
1021 gtgaaactac aatcttccat catgaagaga agggatattt ttggggttgt attttacgat
1081 gaaggtactg gaaatggtgg gggtttttat agcagtagac agtcagtcag taagtagtat
1141 gcttgttgta ttacccaaac cagatcaatc caaagaaagc ctgacagaca gccatcaata
1201 gatactactt cgtactatag ttacccacct aaccatatta ctcaaaaagc atctatctat
1261 ccgcgggctt ccatgcatgt cccggtagca aactcctccc accggtgtag tactctttgg
1321 ttagtagtct tgttcaccgg aggactctgc tcctctcctg ctcaggtgct gccccgccct
1381 ccgtcccacc atgacggaag agatgctccg taagccgtcc agttgcaacg aatcctgctc
1441 tgacatcttc gaacgccttc tccctttcgc tcgcttctct gcctctttcc tctcttccct
1501 ttccttcccc tccaaactaa accttcctcc ttttctccat catcctctag gcagttggtt
1561 cttcctgact gtacatatat ccaccacctc ccccctctat tcttccacct cttccatatc
1621 tccttctcca gagttcatac cccccacaat gccacccaag cgcaaaacgc ttaacgctgc
1681 ggctgaggct aacgctcatg ccgacggaca cgccgacgga aacgccgacg gacacgtggc
1741 caatacggcc gcgtcctcga ataatgcgag gttcgctgat ctcactaaca tcgatactcc
1801 gggtctggga cccacaacta cgaccctgct cgtggaacca gcacgctcaa agcgtcaacg
1861 agtgtcccgc gcatgcgacc agtgccgtgc agcccgagag aaatgcgacg gaatacagcc
1921 tgcgtgtttc ccgtgcgttt cccagggaag gtcctgcact tatcaggctt cgccgaaaaa
1981 gaggggagtt caaaccggtt atattcgtac gctggagctc gccctcgcct ggatgtttga
2041 aaatgtcgcg cgttccgaag atgccttgca taacctcctc gtccgtgacg ccggacaagg
2101 atcagctctg ctcgttggta aagattcgcc ggctgccgag cgactccatg cccgttgggc
2161 tactagccgt gtcaataaga gcattacccg cctcctcagg tttgaaagcg gcgggccggc
2221 cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga
2281 ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggtt
2341 cgttatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
2401 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
2461 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
2521 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
2581 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
2641 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
2701 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
2761 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
2821 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
2881 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
2941 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
3001 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
3061 gccaaagaag aagcggaagg tctaagctta cttctttatc ggttctctct tacgactttt
3121 tgaatggaac gtttccttct tctcaggcgg gcctatcttt gggccgaagc tcttttcctt
3181 gtactgtagg acctggttga taatgattcc caaaaagaca tccagcatgt cagttacttg
3241 cattcgtcag tctatacaaa agcaatggtt tagagaaatt ttgaacttta tacatggttt
3301 tatttgttgc ttcacggccg taccttctgg aaatccacgg taggagtgtc aatttgcgtt
3361 tttgataatc cttccaaggt tcttctcgaa gtagttgttc tataattgct tcacagctac
3421 catggaacat cccaagcacc gctactagaa ttcgagctcg gagtggatcc cccgggctgc
3481 aggaattcga gctctgtaca gtgaccggtg actctttctg gcatgcggag agacggacgg
3541 acgcagagag aagggctgag taataagcgc cactgcgcca gacagctctg gcggctctga
3601 ggtgcagtgg atgattatta atccgggacc ggccgcccct ccgccccgaa gtggaaaggc
3661 tggtgtgccc ctcgttgacc aagaatctat tgcatcatcg gagaatatgg agcttcatcg
3721 aatcaccggc agtaagcgaa ggagaatgtg aagccagggg tgtatagccg tcggcgaaat
3781 agcatgccat taacctaggt acagaagtcc aattgcttcc gatctggtaa aagattcacg
3841 agatagtacc ttctccgaag taggtagagc gagtacccgg cgcgtaagct ccctaattgg
3901 cccatccggc atctgtaggg cgtccaaata tcgtgcctct cctgctttgc ccggtgtatg
3961 aaaccggaaa ggccgctcag gagctggcca gcggcgcaga ccgggaacac aagctggcag
4021 tcgacccatc cggtgctctg cactcgacct gctgaggtcc ctcagtccct ggtaggcagc
4081 tttgccccgt ctgtccgccc ggtgtgtcgg cggggttgac aaggtcgttg cgtcagtcca
4141 acatttgttg ccatattttc ctgctctccc caccagctgc tcttttcttt tctctttctt
4201 ttcccatctt cagtatattc atcttcccat ccaagaacct ttatttcccc taagtaagta
4261 ctttgctaca tccatactcc atccttccca tcccttattc ctttgaacct ttcagttcga
4321 gctttcccac ttcatcgcag cttgactaac agctaccccg cttgagcaga catcaccatg
4381 cctcaatcct gggaagaact ggccgctgat aagcgcgccc gcctcgcaaa aaccatccct
4441 gatgaatgga aagtccagac gctgcctgcg gatgacagcg ttattgattt cccaaagaaa
4501 tcggggatcc tttcagaggc cgaactgaag atcacagagg cctccgctgc agatcttgtg
4561 tccaagctgg cggccggaga gttgacctcg gtggaagtta cgctagcatt ctgtaaacgg
4621 gcagcaatcg cccagcagtt agtagggtcc cctctacctc tcagggagat gtaacaacgc
4681 caccttatgg gactatcaag ctgacgctgg cttctgtgca gacaaactgc gcccacgagt
4741 tcttccctga cgccgctctc gcgcaggcaa gggaactcga tgaatactac gcaaagcaca
4801 agacacccgt tggtccactc catggcctcc ccatctctct caaagaccag cttcgagtca
4861 aggtacaccg ttgcccctaa gtcgttagat gtcccttttt gtcagctaac atatgccacc
4921 agggctacga aacatcaatg ggctacatct catggctaaa caagtacgac gaaggggact
4981 cggttctgac aaccatgctc cgcaaagccg gtgccgtttt ctacgtcaag acctctgtcc
5041 cgcagaccct gatggtctgc gagacagtca acaacatcat cgggcgcacc gtcaacccac
5101 gcaacaagaa ctggtcgtgc ggcggcagtt ctggtggtga gggtgcgatc gttgggattc
5161 gtggtggcgt catcggtgta ggaacggata tcggtggctc gattcgagtg ccggccgcgt
5221 tcaacttcct gtacggtcta aggccgagtc atgggcggct gccgtatgca aagatggcga
5281 acagcatgga gggtcaggag acggtgcaca gcgttgtcgg gccgattacg cactctgttg
5341 agggtgagtc cttcgcctct tccttctttt cctgctctat accaggcctc cactgtcctc
5401 ctttcttgct ttttatacta tatacgagac gggcagtcac tgatgaagta atgttagacc
5461 tccgcctctt caccaaatcc gtcctcggtc aggagccatg gaaatacgac tccaaggtca
5521 tccccatgcc ctggcgccag tccgagtcgg acattattgc ctccaagatc aagaacggcg
5581 ggctcaatat cggctactac aacttcgacg gcaatgtcct tccacaccct cctatcctgc
5641 gcggcgtgga aaccaccgtc gccgcactcg ccaaagccgg tcacaccgtg accccgtgga
5701 cgccatacaa gcacgatttc ggccacgatc tcatctccca tatctacgcg gctgacggca
5761 gcgccgacgt aatgcgcgat atcagtgcat ccggcgagcc ggcgattcca aatatcaaag
5821 acctactgaa cccgaacatc aaagctgtta acatgaacga gctctgggac acgcatctcc
5881 agaagtggaa ttaccagatg gagtaccttg agaaatggcg ggaggctgaa gaaaaggccg
5941 ggaaggaact ggacgccatc atcgcgccga ttacgcctac cgctgcggta cggcatgacc
6001 agttccggta ctatgggtat gcctctgtga tcaacctgct ggatttcacg agcgtggttg
6061 ttccggttac ctttgcggat aagaacatcg ataagaagaa tgagagtttc aaggcggtta
6121 gtgagcttga tgccctcgtg caggaagagt atgatccgga ggcgtaccat ggggcaccgg
6181 ttgcagtgca ggttatcgga cggagactca gtgaagagag gacgttggcg attgcagagg
6241 aagtggggaa gttgctggga aatgtggtga ctccataggc ttctaataag tgtcagatag
6301 caatttgcac aagaaatcaa taccagcaac tgtaaataag cgctgaagtg accatgccat
6361 gctacgaaag agcagaaaaa aacctgccgt agaaccgaag agatatgaca cgcttccatc
6421 tctcaaagga agaatccctt cagggttgcg tttccagtct agacacgtat aacggcacaa
6481 gtgtctctca ccaaatgggt tatatctcaa atgtgatcta aggatggaaa gcccagaata
6541 ttggctgggt tgatggctgc ttcgagtgca gtctcatgct gccacaggtg actctggatg
6601 gccccatacc actcaaccca tggtacgagc ggtacgcttt acgaattccc atggggaggg
6661 gtaatcgatt atcctcggat aaacacttat cccagggtaa tcacttatcc ctggataaac
6721 aattatcctc gggtaatcgc ttatccccat cgtataatgt ctccaggttg tctcagcata
6781 aacaccccgc ccccgctcag gcacacagga agagagctca ggtcgtttcc attgcgtcca
6841 tactcttcac tcattgtcat ctgcaggaga acttcccctg tccctttgcc aagccctctc
6901 ttcgtcgttg tccacgcctt caagttttca ccattatttt tctagacacc aatggcctcc
6961 tccgaggacg tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac
7021 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
7081 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
7141 cagttccagt acggctccaa ggtgtacgtg aagcaccccg ccgacatccc cgactacaag
7201 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
7261 gtggtgaccg tgacccagga ctcctccctg caggacggct ccttcatcta caaggtgaag
7321 ttcatcggcg tgaacttccc ctccgacggc cccgtaatgc agaagaaaac tatgggctgg
7381 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccacaag
7441 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagtccat ctacatggcc
7501 aagaagcccg tgcagctgcc cggctactac tacgtggact ccaagctgga catcacctcc
7561 cacaacgagg actacaccat cgtggagcag tacgagcgcg ccgagggccg ccaccacctg
7621 ttcctgactc acggtatgga cgaattgtac aagtcgaagc tgtaagcttg tgcttctaag
7681 gtatgagtcg caaaattgtt ttttattttt ggtcttgagt ctaatatgct cgcagctctt
7741 gcgttgtata tggtcgttgt cgcgtatttt ctgttgtatt aaaagatcaa acgagatcaa
7801 gggatggctc gcgggctgtc tctcgcacta ggaggaagaa tgcctgaaaa aggaactttg
7861 attttagctg tggaatagag atggcttgtt tgaggacgct tgtcgcttgg cgcagggact
7921 tgaatggcag cttgtggaaa ccgaaggcga gaaaagtcga cggatactgt acgtggttct
7981 attgccagtg cggtggaagc ttccttctca tatagttcaa tccttctttg aatctgtttg
8041 tttcatattt ggactgtttc attctctgct tgcgcattct catcttcgag aacgactgca
8101 gggattgttg gttctgtgga gctgatgagc gcgccttgac cacccttgtt cttgttttgc
8161 tcttttgttc tcatttaacc cgtttctccc ttccaaccct ttgaccttgc aacattgtct
8221 cccagcgcgt tgccaaagcg aacttgatat cagtatagta tgaccaagta gtctaccaaa
8281 ataaatttta gtacagtatt gctagttata caagataaat tttgtacaac aatgattatg
8341 cagagctggt attctgtaag gagtaaattc atgcaggtac agagcgtcca cgtcaggcac
8401 acgcgcttta gtgctgggta cacattatgc tacctttggg gcaaggtgct attctaggta
8461 gggtatgcct agcaatgcca tgatcttatg acctcagtaa tctgaccttg gatttaagat
8521 cggagttctc aaaccctgcg cgctcagaga attcgcatgc ggagacttta gacatacctc
8581 tcgttgccga caaatcggca ttccgtttcg gtcacggacg taagagttca acaaagccgc
8641 tcacctgtcc cgttcggcgc ttgagtttcc acttcaaaag cacaaccgct ccgatatttc
8701 cattgccacc gaccaacaaa aggttcatat cgcgcaatag tcgtccaact ctcactccgc
8761 ccgcactcga tggcgaagga tggaagaata tccgagcggc atcggataaa gagatctggt
8821 tcttttcgtg aagtaacgta gggaaccgac ggaaactgta gagctatagg aatttagtat
8881 atcagaagtc aattgagaga agtgtatagt tagtaactca cttgtgccaa caggtatttc
8941 caaccgttct tgttcaaatt tcgtcaagtc accatctcta tgtgcaaaac tggatcatac
9001 aacagctctt attgtagctg catctcgctc tcagagatca tcctatcttc gtcgtcatcc
9061 accaacacag gtgggagtgc tagttgatcc atgctgaggt caagggacac agacggaatg
9121 ggtgtagaac gcctcaaaac aggtaaggta tcaggatcgg ttgtccaggg ggaatccaaa
9181 ccaggttcca ccttgattca gagaataagt atgacgtatt cttggcaatg tttaagcttg
9241 agatgagccg atgtcaaact gggagacatg atgaaggagg aaatgttgct ttggtggggg
9301 agacactata aataccaaag agtgaatggt tgataccatt tggggaagga gtgtctatcg
9361 cagtctctgc agacggcata tcggatagcc tgcaggcgct tgtggaggat gcacatgtga
9421 ccgagggaca cgaagtgatc cgtttaaact atcagtgttt gacaggatat attggcgggt
9481 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
9541 tttatccgtt cgtccatttg tatgtgccag ccgcctttgc gacgctcacc gggctggttg
9601 ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga
9661 agccgtgtgc gagacaccgc ggccgccggc gttgtggata cctcgcggaa aacttggccc
9721 tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt
9781 tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa
9841 tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga
9901 taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat
9961 ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg
10021 ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc
10081 gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg
10141 gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc
10201 tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc ggccgcgaac
10261 ggcctcaccc caaaaatggc agcgctggcc aattcccgag tgcgcggaac ccctatttgt
10321 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
10381 cttcaataat attgaaaaag gaagagtatg gctaaaatga gaatatcacc ggaattgaaa
10441 aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta
10501 tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa
10561 gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg
10621 cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt
10681 gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt
10741 atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc
10801 tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg
10861 gccgatgtgg attgcgaaaa ctgggaagag gacactccat ttaaagatcc gcgcgagctg
10921 tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg
10981 ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga
11041 agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgct cagggaggat
11101 atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg
11161 gagaaaataa aatattatat tttactggat gaattgtttt agctgtcaga ccaagtttac
11221 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
11281 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
11341 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
11401 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
11461 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
11521 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
11581 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
11641 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
11701 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
11761 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
11821 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
11881 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
11941 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggcagat
12001 cctagatgtg gcgcaacgat gccggcgaca agcaggagcg caccgacttc ttccgcatca
12061 agtgttttgg ctctcaggcc gaggcccacg gcaagtattt gggcaagggg tcgctggtat
12121 tcgtgcaggg caagattcgg aataccaagt acgagaagga cggccagacg gtctacggga
12181 ccgacttcat tgccgataag gtggattatc tggacaccaa ggcaccaggc gggtcaaatc
12241 aggaataagg gcacattgcc ccggcgtgag tcggggcaat cccgcaagga gggtgaatga
12301 atcggacgtt tgaccggaag gcatacaggc aagaactgat cgacgcgggg ttttccgccg
12361 aggatgccga aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa accttccagt
12421 ccgtcggctc gatggtccag caagctacgg ccaagatcga gcgcgacagc gtgcaactgg
12481 ctccccctgc cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt cttgaacagg
12541 aggcggcagg tttggcgaag tcgatgacca tcgacacgcg aggaactatg acgaccaaga
12601 agcgaaaaac cgccggcgag gacctggcaa aacaggtcag cgaggccaag caggccgcgt
12661 tgctgaaaca cacgaagcag cagatcaagg aaatgcagct ttccttgttc gatattgcgc
12721 cgtggccgga cacgatgcga gcgatgccaa acgacacggc ccgctctgcc ctgttcacca
12781 cgcgcaacaa gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc cacgtcaaca
12841 aggacgtgaa gatcacctac accggcgtcg agctgcgggc cgacgatgac gaactggtgt
12901 ggcagcaggt gttggagtac gcgaagcgca cccctatcgg cgagccgatc accttcacgt
12961 tctacgagct ttgccaggac ctgggctggt cgatcaatgg ccggtattac acgaaggccg
13021 aggaatgcct gtcgcgccta caggcgacgg cgatgggctt cacgtccgac cgcgttgggc
13081 acctggaatc ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc aagaaaacgt
13141 cccgttgcca ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc gaccactaca
13201 cgaaattcat atgggagaag taccgcaagc tgtcgccgac ggcccgacgg atgttcgact
13261 atttcagctc gcaccgggag ccgtacccgc tcaagctgga aaccttccgc ctcatgtgcg
13321 gatcggattc cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc tgcgaagagt
13381 tgcgaggcag cggcctggtg gaacacgcct gggtcaatga tgacctggtg cattgcaaac
13441 gctagggcct tgtggggtca gttccggctg ggggttcagc agcccctgct cggatctgtt
13501 ggaccggaca gtagtcatgg ttgatgggct gcctgtatcg agtggtgatt ttgtgccgag
13561 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt
13621 gacgcttaga caacttaata acacattgcg gacgttttta atgtactggg gttgaacact
13681 ct
//
