pCMV-hA3B-BE3

LOCUS       pCMV-hA3B-BE3                8991 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses hA3B-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-hA3B-BE3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8991)
  AUTHORS   Wang X, Li J, Wang Y, Yang V, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol.,  2018
REFERENCE   2  (bases 1 to 8991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8991
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        141..520
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        521..724
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     675..695
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     766..785
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        766..784
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1992..6092
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6105..6353
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6366..6386
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6395..6412
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6435..6452)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6441..6665
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6736..6752)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6736..6752)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6749..6771)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6760..6776
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6784..6814)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6829..6850
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6967..6984)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7138..7726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7218..7237)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7897..8757)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8520..8539
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8758..8862)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8941..8960)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
       61 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
      121 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
      181 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      241 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      301 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      361 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      421 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      481 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      541 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      601 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      661 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      721 agctggttta gtgaaccgtc agatccgcta gagatccgcg gccgctaata cgactcacta
      781 tagggagagc cgccaccatg aatccacaga tcagaaatcc gatggagcgg atgtatcgag
      841 acacattcta cgacaacttt gaaaacgaac ccatcctcta tggtcggagc tacacttggc
      901 tgtgctatga agtgaaaata aagaggggcc gctcaaatct cctttgggac acaggggtct
      961 ttcgaggcca ggtgtatttc aagcctcagt accacgcaga aatgtgcttc ctctcttggt
     1021 tctgtggcaa ccagctgcct gcttacaagt gtttccagat cacctggttt gtatcctgga
     1081 ccccctgccc ggactgtgtg gcgaagctgg ccgaattcct gtctgagcac cccaatgtca
     1141 ccctgaccat ctccgccgcc cgcctctact actactggga aagagattac cgaagggcgc
     1201 tctgcaggct gagtcaggca ggagcccgcg tgaagatcat ggactatgaa gaatttgcat
     1261 actgctggga aaactttgtg tacaatgaag gtcagcaatt catgccttgg tacaaattcg
     1321 atgaaaatta tgcattcctg caccgcacgc taaaggagat tctcagatac ctgatggatc
     1381 cagacacatt cactttcaac tttaataatg accctttggt ccttcgacgg cgccagacct
     1441 acttgtgcta tgaggtggag cgcctggaca atggcacctg ggtcctgatg gaccagcaca
     1501 tgggctttct atgcaacgag gctaagaatc ttctctgtgg cttttacggc cgccatgcgg
     1561 agctgcgctt cttggacctg gttccttctt tgcagttgga cccggcccag atctacaggg
     1621 tcacttggtt catctcctgg agcccctgct tctcctgggg ctgtgccggg gaagtgcgtg
     1681 cgttccttca ggagaacaca cacgtgagac tgcgcatctt cgctgcccgc atctatgatt
     1741 acgaccccct atataaggag gcgctgcaaa tgctgcggga tgctggggcc caagtctcca
     1801 tcatgaccta cgatgagttt gagtactgct gggacacctt tgtgtaccgc cagggatgtc
     1861 ccttccagcc ctgggatgga ctagaggagc acagccaagc cctgagtggg aggctgcggg
     1921 ccattctcca gaatcaggga aacagcggca gcgagactcc cgggacctca gagtccgcca
     1981 cacccgaaag tgataaaaag tattctattg gtttagccat cggcactaat tccgttggat
     2041 gggctgtcat aaccgatgaa tacaaagtac cttcaaagaa atttaaggtg ttggggaaca
     2101 cagaccgtca ttcgattaaa aagaatctta tcggtgccct cctattcgat agtggcgaaa
     2161 cggcagaggc gactcgcctg aaacgaaccg ctcggagaag gtatacacgt cgcaagaacc
     2221 gaatatgtta cttacaagaa atttttagca atgagatggc caaagttgac gattctttct
     2281 ttcaccgttt ggaagagtcc ttccttgtcg aagaggacaa gaaacatgaa cggcacccca
     2341 tctttggaaa catagtagat gaggtggcat atcatgaaaa gtacccaacg atttatcacc
     2401 tcagaaaaaa gctagttgac tcaactgata aagcggacct gaggttaatc tacttggctc
     2461 ttgcccatat gataaagttc cgtgggcact ttctcattga gggtgatcta aatccggaca
     2521 actcggatgt cgacaaactg ttcatccagt tagtacaaac ctataatcag ttgtttgaag
     2581 agaaccctat aaatgcaagt ggcgtggatg cgaaggctat tcttagcgcc cgcctctcta
     2641 aatcccgacg gctagaaaac ctgatcgcac aattacccgg agagaagaaa aatgggttgt
     2701 tcggtaacct tatagcgctc tcactaggcc tgacaccaaa ttttaagtcg aacttcgact
     2761 tagctgaaga tgccaaattg cagcttagta aggacacgta cgatgacgat ctcgacaatc
     2821 tactggcaca aattggagat cagtatgcgg acttattttt ggctgccaaa aaccttagcg
     2881 atgcaatcct cctatctgac atactgagag ttaatactga gattaccaag gcgccgttat
     2941 ccgcttcaat gatcaaaagg tacgatgaac atcaccaaga cttgacactt ctcaaggccc
     3001 tagtccgtca gcaactgcct gagaaatata aggaaatatt ctttgatcag tcgaaaaacg
     3061 ggtacgcagg ttatattgac ggcggagcga gtcaagagga attctacaag tttatcaaac
     3121 ccatattaga gaagatggat gggacggaag agttgcttgt aaaactcaat cgcgaagatc
     3181 tactgcgaaa gcagcggact ttcgacaacg gtagcattcc acatcaaatc cacttaggcg
     3241 aattgcatgc tatacttaga aggcaggagg atttttatcc gttcctcaaa gacaatcgtg
     3301 aaaagattga gaaaatccta acctttcgca taccttacta tgtgggaccc ctggcccgag
     3361 ggaactctcg gttcgcatgg atgacaagaa agtccgaaga aacgattact ccatggaatt
     3421 ttgaggaagt tgtcgataaa ggtgcgtcag ctcaatcgtt catcgagagg atgaccaact
     3481 ttgacaagaa tttaccgaac gaaaaagtat tgcctaagca cagtttactt tacgagtatt
     3541 tcacagtgta caatgaactc acgaaagtta agtatgtcac tgagggcatg cgtaaacccg
     3601 cctttctaag cggagaacag aagaaagcaa tagtagatct gttattcaag accaaccgca
     3661 aagtgacagt taagcaattg aaagaggact actttaagaa aattgaatgc ttcgattctg
     3721 tcgagatctc cggggtagaa gatcgattta atgcgtcact tggtacgtat catgacctcc
     3781 taaagataat taaagataag gacttcctgg ataacgaaga gaatgaagat atcttagaag
     3841 atatagtgtt gactcttacc ctctttgaag atcgggaaat gattgaggaa agactaaaaa
     3901 catacgctca cctgttcgac gataaggtta tgaaacagtt aaagaggcgt cgctatacgg
     3961 gctggggacg attgtcgcgg aaacttatca acgggataag agacaagcaa agtggtaaaa
     4021 ctattctcga ttttctaaag agcgacggct tcgccaatag gaactttatg cagctgatcc
     4081 atgatgactc tttaaccttc aaagaggata tacaaaaggc acaggtttcc ggacaagggg
     4141 actcattgca cgaacatatt gcgaatcttg ctggttcgcc agccatcaaa aagggcatac
     4201 tccagacagt caaagtagtg gatgagctag ttaaggtcat gggacgtcac aaaccggaaa
     4261 acattgtaat cgagatggca cgcgaaaatc aaacgactca gaaggggcaa aaaaacagtc
     4321 gagagcggat gaagagaata gaagagggta ttaaagaact gggcagccag atcttaaagg
     4381 agcatcctgt ggaaaatacc caattgcaga acgagaaact ttacctctat tacctacaaa
     4441 atggaaggga catgtatgtt gatcaggaac tggacataaa ccgtttatct gattacgacg
     4501 tcgatcacat tgtaccccaa tcctttttga aggacgattc aatcgacaat aaagtgctta
     4561 cacgctcgga taagaaccga gggaaaagtg acaatgttcc aagcgaggaa gtcgtaaaga
     4621 aaatgaagaa ctattggcgg cagctcctaa atgcgaaact gataacgcaa agaaagttcg
     4681 ataacttaac taaagctgag aggggtggct tgtctgaact tgacaaggcc ggatttatta
     4741 aacgtcagct cgtggaaacc cgccaaatca caaagcatgt tgcacagata ctagattccc
     4801 gaatgaatac gaaatacgac gagaacgata agctgattcg ggaagtcaaa gtaatcactt
     4861 taaagtcaaa attggtgtcg gacttcagaa aggattttca attctataaa gttagggaga
     4921 taaataacta ccaccatgcg cacgacgctt atcttaatgc cgtcgtaggg accgcactca
     4981 ttaagaaata cccgaagcta gaaagtgagt ttgtgtatgg tgattacaaa gtttatgacg
     5041 tccgtaagat gatcgcgaaa agcgaacagg agataggcaa ggctacagcc aaatacttct
     5101 tttattctaa cattatgaat ttctttaaga cggaaatcac tctggcaaac ggagagatac
     5161 gcaaacgacc tttaattgaa accaatgggg agacaggtga aatcgtatgg gataagggcc
     5221 gggacttcgc gacggtgaga aaagttttgt ccatgcccca agtcaacata gtaaagaaaa
     5281 ctgaggtgca gaccggaggg ttttcaaagg aatcgattct tccaaaaagg aatagtgata
     5341 agctcatcgc tcgtaaaaag gactgggacc cgaaaaagta cggtggcttc gatagcccta
     5401 cagttgccta ttctgtccta gtagtggcaa aagttgagaa gggaaaatcc aagaaactga
     5461 agtcagtcaa agaattattg gggataacga ttatggagcg ctcgtctttt gaaaagaacc
     5521 ccatcgactt ccttgaggcg aaaggttaca aggaagtaaa aaaggatctc ataattaaac
     5581 taccaaagta tagtctgttt gagttagaaa atggccgaaa acggatgttg gctagcgccg
     5641 gagagcttca aaaggggaac gaactcgcac taccgtctaa atacgtgaat ttcctgtatt
     5701 tagcgtccca ttacgagaag ttgaaaggtt cacctgaaga taacgaacag aagcaacttt
     5761 ttgttgagca gcacaaacat tatctcgacg aaatcataga gcaaatttcg gaattcagta
     5821 agagagtcat cctagctgat gccaatctgg acaaagtatt aagcgcatac aacaagcaca
     5881 gggataaacc catacgtgag caggcggaaa atattatcca tttgtttact cttaccaacc
     5941 tcggcgctcc agccgcattc aagtattttg acacaacgat agatcgcaaa cgatacactt
     6001 ctaccaagga ggtgctagac gcgacactga ttcaccaatc catcacggga ttatatgaaa
     6061 ctcggataga tttgtcacag cttgggggtg actctggtgg ttctactaat ctgtcagata
     6121 ttattgaaaa ggagaccggt aagcaactgg ttatccagga atccatcctc atgctcccag
     6181 aggaggtgga agaagtcatt gggaacaagc cggaaagcga tatactcgtg cacaccgcct
     6241 acgacgagag caccgacgag aatgtcatgc ttctgactag cgacgcccct gaatacaagc
     6301 cttgggctct ggtcatacag gatagcaacg gtgagaacaa gattaagatg ctctctggtg
     6361 gttctcccaa gaagaagagg aaagtctaac cggtcatcat caccatcacc attgagttta
     6421 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     6481 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     6541 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     6601 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     6661 tatggcttct gaggcggaaa gaaccagctg gggctcgata ccgtcgacct ctagctagag
     6721 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6781 acacaacata cgagccggaa gcataaagtg taaagcctag ggtgcctaat gagtgagcta
     6841 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6901 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6961 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7021 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7081 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7141 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7201 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7261 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7321 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7381 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7441 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7501 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7561 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7621 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7681 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7741 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7801 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7861 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7921 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7981 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8041 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8101 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8161 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8221 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8281 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8341 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8401 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8461 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8521 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8581 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8641 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8701 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8761 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8821 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8881 gccacctgac gtcgacggat cgggagatcg atctcccgat cccctagggt cgactctcag
     8941 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt g
//