| 质粒名称: | pmGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 117926 |
| 存储实验室: | Kumiko Ui-Tei |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | Thermo Fisher Scientific |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pmGFP was a gift from Kumiko Ui-Tei (Addgene plasmid # 117926 ; http://n2t.net/addgene:117926 ; RRID:Addgene_117926) |
| 参考文献部分: | Differential Binding of Three Major Human ADAR Isoforms to Coding and Long Non-Coding Transcripts. Galipon J, Ishii R, Suzuki Y, Tomita M, Ui-Tei K. Genes (Basel). 2017 Feb 11;8(2). pii: genes8020068. doi: 10.3390/genes8020068. 10.3390/genes8020068 PubMed 28208661 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pmGFP 6108 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Control plasmid expressing myc-GFP..
ACCESSION .
VERSION .
KEYWORDS pmGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6108)
AUTHORS Galipon J, Ishii R, Suzuki Y, Tomita M, Ui-Tei K
TITLE Differential Binding of Three Major Human ADAR Isoforms to Coding
and Long Non-Coding Transcripts.
JOURNAL Genes (Basel). 2017 Feb 11;8(2). pii: genes8020068. doi:
10.3390/genes8020068.
PUBMED 28208661
REFERENCE 2 (bases 1 to 6108)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6108
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 123..502
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 503..706
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 657..677
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 751..770
/label=T7
/note="T7 promoter, forward primer"
promoter 751..769
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 797..826
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 836..1552
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(881..902)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1142..1161)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1489..1510
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(1587..1604)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 1593..1817
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1863..2291
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1950..1969)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2160..2181
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(2300..2320)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2305..2634
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2485..2620
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2547..2566
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2701..3495
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2755..2774)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3365..3384
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 3669..3790
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3706..3725)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3760..3779
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(3839..3855)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3839..3855)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3852..3874)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3863..3879
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3887..3917)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3932..3953
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4070..4087)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4241..4829)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4321..4340)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5000..5860)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5623..5642
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5861..5965)
/gene="bla"
/label=AmpR promoter
primer_bind complement(6040..6059)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
61 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
121 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
181 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
241 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
301 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
361 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
421 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
481 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
541 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
601 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
661 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag
721 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
781 tggctagccc accatggaac aaaaactcat ctcagaagag gatctgtccg gcggcatggt
841 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
901 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
961 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
1021 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
1081 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
1141 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
1201 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
1261 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
1321 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
1381 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
1441 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
1501 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtagtctag
1561 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
1621 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
1681 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
1741 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
1801 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
1861 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
1921 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
1981 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
2041 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
2101 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
2161 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
2221 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
2281 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
2341 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
2401 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
2461 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
2521 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
2581 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
2641 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
2701 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
2761 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
2821 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
2881 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
2941 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
3001 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
3061 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
3121 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
3181 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
3241 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
3301 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
3361 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
3421 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
3481 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
3541 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
3601 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
3661 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
3721 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
3781 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
3841 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
3901 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
3961 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
4021 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
4081 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
4141 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
4201 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
4261 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
4321 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
4381 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
4441 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
4501 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
4561 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
4621 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
4681 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
4741 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
4801 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
4861 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
4921 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
4981 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
5041 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
5101 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
5161 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
5221 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
5281 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
5341 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
5401 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
5461 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
5521 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
5581 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
5641 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
5701 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
5761 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
5821 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
5881 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
5941 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
6001 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
6061 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctga
//
