| 质粒名称: | GFP-rab7 DN |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 12660 |
| 存储实验室: | Richard Pagano |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4700 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | RAB7 (Homo sapiens) |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 693 bp |
| 基因突变: | T22N |
| GenBank编号 | NM_004637 |
| 标签/融合蛋白: | GFP（N端） HA（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BAMHI(未破坏) |
| 3' 克隆位点: | BAMHI(未破坏) |
| 5' 测序引物 | EGFP-C |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | GFP-rab7 DN was a gift from Richard Pagano (Addgene plasmid # 12660) |
| 参考文献部分: | Rab proteins mediate Golgi transport of caveola-internalized glycosphingolipids and correct lipid trafficking in Niemann-Pick C cells. Choudhury A, Dominguez M, Puri V, Sharma DK, Narita K, Wheatley CL, Marks DL, Pagano RE. J Clin Invest. 2002 Jun . 109(12):1541-50. 10.1172/JCI15420 PubMed 12070301 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS GFP-rab7 DN Sequencing Result 5418 bp DNA SYN
DEFINITION GFP-rab7 DN Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5418
/organism="GFP-rab7 DN Sequencing Result"
/mol_type="other DNA"
promoter 1..517
/label="CMV_immearly_promoter"
misc_feature 20..307
/label="CAG_enhancer"
misc_feature 474..494
/label="CMV_fwd_primer"
promoter 475..544
/label="CMV_promoter"
CDS 568..2031
/label="ORF frame 1"
gene 571..1284
/label="EGFP"
/gene="EGFP"
misc_feature complement(613..634)
/label="EGFP_N_primer"
misc_feature 751..780
/label="Y66 (EGFP)"
misc_feature 1221..1242
/label="EGFP_C_primer"
misc_feature 1354..1380
/label="HA"
terminator 2054..2282
/label="SV40_PA_terminator"
misc_feature 2252..2271
/label="EBV_rev_primer"
rep_origin complement(2421..2727)
/label="f1_origin"
promoter 2806..2834
/label="AmpR_promoter"
misc_feature complement(2900..2920)
/label="pBABE_3_primer"
misc_feature complement(2906..3121)
/label="SV40_enhancer"
promoter 2918..3186
/label="SV40_promoter"
rep_origin 3085..3162
/label="SV40_origin"
misc_feature 3147..3166
/label="SV40pro_F_primer"
CDS 3269..4063
/label="ORF frame 2"
gene 3272..4060
/label="NeoR/KanR"
/gene="NeoR/KanR"
CDS complement(3578..4114)
/label="ORF frame 3"
terminator 4238..4507
/label="TK_PA_terminator"
rep_origin 4655..5274
/label="pBR322_origin"
promoter 5383..517
/label="CMV_immearly_promoter"
ORIGIN
1 ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA
61 CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC
121 TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA
181 AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG
241 GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT
301 AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG
361 GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG
421 GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT
481 GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTGGTTTAG TGAACCGTCA
541 GATCCGCTAG CGCTACCGGT CGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG
601 GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC
661 GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC
721 GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC
781 TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA
841 GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC
901 GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC
961 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC
1021 TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC
1081 ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC
1141 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC
1201 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT
1261 CTCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCTC GAGCTCAAGC TTCGAATTCT
1321 GCAGTCGACG GTACCGCGGG CCCGGGATCC ATGTACCCAT ACGATGTTCC GGATTACGCT
1381 TACCCATACG ATGTTCCGGA TTACGCTGGT ACCTCTAGGA AGAAAGTGTT GCTGAAGGTT
1441 ATCATCCTGG GAGATTCTGG AGTCGGGAAG AACTCACTCA TGAACCAGTA TGTGAATAAG
1501 AAATTCAGCA ATCAGTACAA AGCCACAATA GGAGCTGACT TTCTGACCAA GGAGGTGATG
1561 GTGGATGACA GGCTAGTCAC AATGCAGATA TGGGACACAG CAGGACAGGA ACGGTTCCAG
1621 TCTCTCGGTG TGGCCTTCTA CAGAGGTGCA GACTGCTGCG TTCTGGTATT TGATGTGACT
1681 GCCCCCAACA CATTCAAAAC CCTAGATAGC TGGAGAGATG AGTTTCTCAT CCAGGCCAGT
1741 CCCCGAGATC CTGAAAACTT CCCATTTGTT GTGTTGGGAA ACAAGATTGA CCTCGAAAAC
1801 AGACAAGTGG CCACAAAGCG GGCACAGGCC TGGTGCTACA GCAAAAACAA CATTCCCTAC
1861 TTTGAGACCA GTGCCAAGGA GGCCATCAAC GTGGAGCAGG CGTTCCAGAC GATTGCACGG
1921 AATGCACTTA AGCAGGAAAC GGAGGTGGAG CTGTACAACG AATTTCCTGA ACCTATCAAA
1981 CTGGACAAGA ATGACCGGGC CAAGGCCTCG GCAGAAAGCT GCAGTTGCTG AGGATCCACC
2041 GGATCTAGAT AACTGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA
2101 AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
2161 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA
2221 AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT
2281 TAACGCGTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT
2341 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA
2401 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT
2461 GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC
2521 ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC GGAACCCTAA
2581 AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG AACGTGGCGA GAAAGGAAGG
2641 GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT
2701 AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC GCGTCAGGTG GCACTTTTCG
2761 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC
2821 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTCCTGA
2881 GGCGGAAAGA ACCAGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC
2941 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG
3001 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC
3061 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT
3121 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT
3181 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAGATCGA
3241 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC
3301 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG
3361 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC
3421 CGACCTGTCC GGTGCCCTGA ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC
3481 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
3541 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA
3601 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG
3661 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG
3721 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT
3781 CGCCAGGCTC AAGGCGAGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC
3841 CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG
3901 GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA
3961 GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC
4021 GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC
4081 GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC
4141 TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG
4201 CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCTAGGG GGAGGCTAAC TGAAACACGG
4261 AAGGAGACAA TACCGGAAGG AACCCGCGCT ATGACGGCAA TAAAAAGACA GAATAAAACG
4321 CACGGTGTTG GGTCGTTTGT TCATAAACGC GGGGTTCGGT CCCAGGGCTG GCACTCTGTC
4381 GATACCCCAC CGAGACCCCA TTGGGGCCAA TACGCCCGCG TTTCTTCCTT TTCCCCACCC
4441 CACCCCCCAA GTTCGGGTGA AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT
4501 GCCATAGCCT CAGGTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT
4561 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG
4621 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT
4681 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT
4741 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG
4801 CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT
4861 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC
4921 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG
4981 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA
5041 CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG
5101 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG
5161 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA
5221 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT
5281 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT
5341 GATTCTGTGG ATAACCGTAT TACCGCcatg cattagttat taatagtaat caattacggg
5401 gtcattagtt catagccc
//
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