| 质粒名称: | pMSCVII |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 162750 |
| 存储实验室: | Maki Nakayama |
|
载体骨架基本信息 |
|
| 载体名称: | Modified from pMIGII (removed IRES-EGFP cassette) |
| 载体出品公司: | Dario Vignali (Addgene plasmid # 52107) |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMSCVII was a gift from Maki Nakayama (Addgene plasmid # 162750 ; http://n2t.net/addgene:162750 ; RRID:Addgene_162750) |
| 参考文献部分: | Multiplex T-cell Stimulation Assay Utilizing a T-cell Activation Reporter-based Detection System. Landry LG, Mann SE, Anderson AM, Nakayama M. Bio Protoc. 2021 Jan 20;11(2):e3883. doi: 10.21769/BioProtoc.3883. eCollection 2021 Jan 20. 10.21769/BioProtoc.3883 PubMed 33732772 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMSCVII 5192 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION MSCV backbone.
ACCESSION .
VERSION .
KEYWORDS pMSCVII
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5192)
TITLE Nakayama lab plasmids
REFERENCE 2 (bases 1 to 5192)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5192
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..517
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
misc_feature 581..922
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 989..1405
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1335..1357
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1373..1389
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
LTR 1587..2101
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 2263..2279
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2287..2317)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2332..2353
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(2470..2487)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2641..3229)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2721..2740)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3400..4260)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4023..4042
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4261..4365)
/gene="bla"
/label=AmpR promoter
primer_bind 4433..4451
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(4489..4511)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 4611..4630
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4824..4846
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 4974..4993
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
ORIGIN
1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
1381 agccctcact ccttctctag gcgccggaat tcagatctta cgtagctagc ggatcccaat
1441 tgctcgagcc tgcaggcatg caagcttcag gtagccggct aacgttaaca accggtacct
1501 ctagaactat agctagcatg cgcaaattta aagcgctgat atcgataaaa taaaagattt
1561 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
1621 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag
1681 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
1741 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag
1801 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
1861 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
1921 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc
1981 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
2041 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
2101 tgggtaacag tttcttgaag ttggagaaca acattctgag ggtaggagtc gaatattaag
2161 taatcctgac tcaattagcc actgttttga atccacatac tccaatactc ctgaaatagt
2221 tcattatgga cagcgcagaa agagctgggg agaattgtga aattgttatc cgctcacaat
2281 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
2341 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
2401 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
2461 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
2521 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
2581 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
2641 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
2701 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
2761 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
2821 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
2881 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
2941 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
3001 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
3061 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
3121 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
3181 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
3241 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
3301 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
3361 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
3421 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
3481 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
3541 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
3601 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
3661 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
3721 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
3781 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
3841 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
3901 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
3961 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
4021 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
4081 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
4141 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
4201 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
4261 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
4321 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
4381 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
4441 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
4501 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
4561 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
4621 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
4681 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
4741 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
4801 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg cgcaaggaat
4861 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
4921 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
4981 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
5041 ccggcgtaga ggcgattagt ccaatttgtt aaagacagga tatcagtggt ccaggctcta
5101 gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata gataaaataa
5161 aagattttat ttagtctcca gaaaaagggg gg
//
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