| 质粒名称: | pAAVLINK-EF1a-HA-5'-CIC |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001693 |
| 产品用途: | 5' AAVLINK plasmid for CIC expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CIC |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-CIC
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-CIC载体质粒基本信息
pAAVLINK-EF1a-HA-5'-CIC质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-CIC载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-CIC质粒载体简介
pAAVLINK-EF1a-HA-5'-CIC质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-CIC 7097 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7097)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 2, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7097
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4237
/codon_start=1
/label=CIC
/translation="KPMKKACTGLSGPGSGSKSPPATRAKALRRRGAGEGDKPEEEDDE
AQQPQPQSGPEEAEEGEEEEAERGPGAEGPPLELHPGDPAPGPAEDPKGDGEAGRWEPS
LSRKTATFKSRAPKKKYVEEHGAGSSGVAGAPEERVRTPEEASGLGVPPRPPTSTRSSS
TDTASEHSADLEDEPAEACGPGPWPPGSTSGSYDLRQLRSQRVLARRGDGLFLPAVVRQ
VRRSQDLGVQFPGDRALTFYEGVPGAGVDVVLDATPPPGALVVGTAVCTCVEPGVAAYR
EGVVVEVATKPAAYKVRLSPGPSSQPGLPGSLPQPPQPLHREPEEAVWVARSSLRLLRP
PWEPETMLRKPPTGPEEEQAEPGATLPPCPAALDPKQPEDAEVSKISFGGNLGTHCEEG
EEKHPPALGTPALLPLPPPQLLSPPPKSPAFVGPGRPGEQPSPCQEGSQGGSRSSSVAS
LEKGTAPAARARTPLTAAQQKYKKGDVVCTPSGIRKKFNGKQWRRLCSRDGCMKESQRR
GYCSRHLSMRTKEMEGLADSGPGGAGRPAAVAAREGSTEFDWGDETSRDSEASSVAARG
DSRPRLVAPADLSRFEFDECEAAVMLVSLGSSRSGTPSFSPVSTQSPFSPAPSPSPSPL
FGFRPANFSPINASPVIQRTAVRSRHLSASTPKAGVLTPPDLGPHPPPPAPRERHSSGI
LPTFQTNLTFTVPISPGRRKTELLPHPGALGAPGAGGGGAAPDFPKSDSLDSGVDSVSH
TPTPSTPAGFRAVSPAVPFSRSRQPSPLLLLPPPAGLTSDPGPSVRRVPAVQRDSPVIV
RNPDVPLPSKFPGEVGTAGEVRAGGPGRGCRETPVPPGVASGKPGLPPPLPAPVPITVP
PAAPTAVAQPMPAFGLASSPFQPVAFHPSPAALLPVLVPSSYTSHPAPKKEVIMGRPGT
VWTNVEPRSVAVFPWHSLVPFLAPSQPDPSVQPSEAQQPASHPVASNQSKEPAESAAVA
HERPPGGTGSADPERPPGATCPESPGPGPPHPLGVVESGKGPPPTTEEEASGPPGEPRL
DSETESDHDDAFLSIMSPEIQLPLPPGKRRTQSLSALPKERDSSSEKDGRSPNKREKDH
IRRPMNAFMIFSKRHRALVHQRHPNQDNRTVSKILGEWWYALGPKEKQKYHDLAFQ"
misc_feature 867..872
/label=SDS
misc_feature 870..875
/label=SDS
misc_feature 933..938
/label=SDS
misc_feature 936..941
/label=SDS
misc_feature 939..944
/label=SDS
misc_feature 1179..1184
/label=SDS
misc_feature 1230..1235
/label=SDS
misc_feature 1722..1727
/label=SDS
misc_feature 1938..1943
/label=SDS
misc_feature 2079..2084
/label=SDS
misc_feature 2586..2591
/label=SDS
misc_feature 3855..3860
/label=SDS
misc_feature 3858..3863
/label=SDS
intron 4238..4278
/label=Splicing Donor
misc_feature 4282..4315
/label=lox2272-JT1571
repeat_region 4360..4500
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4360..4489
/label=AAV2 ITR
rep_origin 4575..5030
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5312..5416
/gene="bla"
/label=AmpR promoter
CDS 5417..6277
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6448..7036
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctaa gccaatgaag aaggcatgca ctggcctttc
781 aggtcctggc agtggcagca agtccccccc agccaccagg gccaaggctc tgaggcggcg
841 aggggctggg gagggtgaca agccagagga ggaggatgac gaggcacagc agccgcaacc
901 acagtccggg cccgaagagg ctgaggaagg ggaggaggag gaggctgagc ggggccctgg
961 ggctgaaggt cctccactgg agctgcaccc tggcgacccg gctccaggcc cagcagagga
1021 ccccaaaggg gatggggagg caggccgctg ggagccctca ctcagccgca agacagccac
1081 gttcaagtct cgagcgccca agaagaagta tgtggaggag cacggagctg gcagcagtgg
1141 ggtggctggg gcccctgaag agcgggtgcg gacccctgag gaggccagtg gcctgggggt
1201 gcctccacgg ccacccacct ccactcgttc ctcctccact gacacagcca gcgagcactc
1261 ggcggacctg gaggatgagc cggctgaggc ttgtggtcca ggcccttggc cccctggcag
1321 caccagtggc agctatgacc tgcggcagct gcggtcccag cgggtgctgg ctcggcgtgg
1381 tgatggcctt ttcctgccgg ctgtggtgcg ccaggtgcgc cgaagccagg acctgggcgt
1441 gcagttccct ggtgaccgag ccctgacttt ctatgagggg gtgccaggcg ctggtgtgga
1501 tgtagttttg gatgccacac ccccaccagg tgccctggtg gtgggcacag ctgtctgtac
1561 ctgtgtggag cccggcgtgg ctgcctaccg ggaaggtgtg gtggtggagg tggccaccaa
1621 gccagctgcc tacaaggtcc gtctcagccc tggccccagc tcccagccag gcctaccagg
1681 cagcctcccg cagcccccac agccactgca ccgtgagcca gaggaggctg tgtgggtggc
1741 ccgctccagc ctacgcctgc tgcgcccccc ctgggaacct gagaccatgc tgaggaagcc
1801 ccctacaggc cctgaggaag agcaggcaga gcctggggcc accctgccac cctgccctgc
1861 tgccctggac cccaaacagc ccgaggatgc tgaggtctct aagatcagct ttggtggcaa
1921 cctgggtact cactgtgagg agggcgagga gaagcaccct ccagccctgg gtaccccagc
1981 cctgctccca ctgcccccac cccagctcct gtcaccacca cccaagtctc cagcctttgt
2041 gggccccggc cgccctggcg agcagccctc gccctgccag gaggggagcc agggcggcag
2101 ccgcagcagc agcgtggcct ccctggaaaa ggggacagca ccggcagccc gggcccgcac
2161 gccactgaca gccgcccagc agaagtacaa gaagggcgat gtggtctgca cacccagcgg
2221 aatacgaaag aagttcaacg gcaagcagtg gcgccggctg tgctcacgag atggctgcat
2281 gaaggagtca cagcggcgag gctactgctc acgccacctg tccatgcgaa ccaaagagat
2341 ggaaggcctg gcagacagtg ggcctggcgg ggcgggccgg cccgcggccg tggcagcccg
2401 tgagggcagc acggagtttg actggggtga tgagacgtcg agggacagtg aggccagcag
2461 tgtggcggct cgtggagact cacggccacg cctggtggcc cctgctgact tgtcacgctt
2521 tgagttcgac gagtgtgagg cggccgtgat gctggtgtcg ctgggcagct cgcgctcagg
2581 cacgccctcc ttctcacccg tctccactca atcgcccttc tcgccagccc catcaccctc
2641 accctcacca ctcttcggct tccgccctgc caactttagc cccatcaatg cctcaccagt
2701 catccagcgc actgcagtcc gcagtcgcca cctgagcgcc agcaccccta aggcaggcgt
2761 gctgacgcca ccagacctgg gcccccaccc accgccacct gccccccgag agcgccactc
2821 ctctggaatc ctacccacct tccagaccaa cctgaccttc accgtgccca tcagccctgg
2881 gcgacggaag acagagctgt tgccgcatcc aggggccttg ggggcccctg gcgcaggggg
2941 tggaggagcc gccccagact ttcccaagag tgacagctta gactctggtg tggactcagt
3001 gtcccacaca cctacaccct ccacgccggc tggcttccgg gccgtgtccc ctgctgtgcc
3061 cttctctcgc tcccgccagc cctcaccatt gctgctgttg ccaccccctg ccggcctgac
3121 ctcggatcca gggccctctg tgcgcagggt gcctgctgtg cagcgggact cacctgttat
3181 tgtccgcaac cctgatgtgc cactgccctc caaattccct ggggaggtgg gcactgctgg
3241 tgaggtgcgg gctgggggac ctgggcgggg ctgccgtgaa accccagtgc cccctggggt
3301 ggccagtggg aagcctggcc tgcccccacc tctgccagcc cccgtgccca tcaccgtgcc
3361 tccagctgca ccaactgccg tggcccagcc gatgcccgcc tttggcctgg cttcttcacc
3421 ctttcagcct gtggccttcc acccctcacc tgctgccctg ttgcccgttt tggtgcccag
3481 cagctatacc agccaccctg cccccaagaa ggaagtcatc atgggccggc ctggaacagt
3541 gtggacgaat gtggaacctc gctctgtggc tgtgttccct tggcactcct tagtcccctt
3601 cctggcaccc agccagcctg acccctccgt gcagccgagc gaggcccagc aacctgccag
3661 ccacccagtg gcctccaacc agagcaaaga acctgctgag tcggcagctg ttgctcatga
3721 acggccacca ggtgggacag ggagtgctga ccctgagcgg ccccctggag ccacatgccc
3781 tgagagccca ggacccggac ccccacaccc tttgggggtg gtggaatctg gtaagggtcc
3841 gcctcccacc acggaggagg aggcctccgg ccccccagga gagccccggc tggacagtga
3901 gacagagagt gaccatgatg atgccttcct ctccatcatg tctcctgaga tccagttgcc
3961 tctaccgccc ggaaaacgtc ggacccagtc cctcagtgcc ctacccaagg aacgggactc
4021 atcttctgag aaggatggac gcagccccaa caagcgggag aaggaccaca tccggcggcc
4081 catgaatgcc ttcatgatct tcagcaagcg gcaccgggcc ctggtccacc agcgtcatcc
4141 caaccaggac aaccggaccg tcagcaagat cctgggcgag tggtggtatg ccctggggcc
4201 caaggagaag cagaagtacc acgacctggc cttccaggta agtatcaagg ttacaagaca
4261 ggtttaagga gaccaataag ctattattcg tataggatac tttatacgaa gttattaagt
4321 tatggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
4381 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
4441 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
4501 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
4561 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
4621 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
4681 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
4741 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
4801 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
4861 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
4921 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
4981 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
5041 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
5101 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
5161 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
5221 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
5281 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
5341 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
5401 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
5461 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
5521 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
5581 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
5641 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
5701 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
5761 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
5821 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
5881 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
5941 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
6001 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
6061 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
6121 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
6181 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
6241 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
6301 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
6361 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
6421 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
6481 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
6541 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
6601 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
6661 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
6721 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
6781 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
6841 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
6901 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
6961 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
7021 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
7081 gccttttgct cacatgt
//
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