pAAVLINKv1-pCALM1-Cre-3'-ABCC6-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-ABCC6-FLAG             7030 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3743
                     /codon_start=1
                     /label=ABCC6
                     /translation="GMNLSGGQKQRLSLARAVYRKAAVYLLDDPLAALDAHVGQHVFNQ
                     VIGPGGLLQGTTRILVTHALHILPQADWIIVLANGAIAEMGSYQELLQRKGALMCLLDQ
                     ARQPGDRGEGETEPGTSTKDPRGTSAGRRPELRRERSIKSVPEKDRTTSEAQTEVPLDD
                     PDRAGWPAGKDSIQYGRVKATVHLAYLRAVGTPLCLYALFLFLCQQVASFCRGYWLSLW
                     ADDPAVGGQQTQAALRGGIFGLLGCLQAIGLFASMAAVLLGGARASRLLFQRLLWDVVR
                     SPISFFERTPIGHLLNRFSKETDTVDVDIPDKLRSLLMYAFGLLEVSLVVAVATPLATV
                     AILPLFLLYAGFQSLYVVSSCQLRRLESASYSSVCSHMAETFQGSTVVRAFRTQAPFVA
                     QNNARVDESQRISFPRLVADRWLAANVELLGNGLVFAAATCAVLSKAHLSAGLVGFSVS
                     AALQVTQTLQWVVRNWTDLENSIVSVERMQDYAWTPKEAPWRLPTCAAQPPWPQGGQIE
                     FRDFGLRYRPELPLAVQGVSFKIHAGEKVGIVGRTGAGKSSLASGLLRLQEAAEGGIWI
                     DGVPIAHVGLHTLRSRISIIPQDPILFPGSLRMNLDLLQEHSDEAIWAALETVQLKALV
                     ASLPGQLQYKCADRGEDLSVGQKQLLCLARALLRKTQILILDEATAAVDPGTELQMQAM
                     LGSWFAQCTVLLIAHRLRSVMDCARVLVMDKGQVAESGSPAQLLAQKGLFYRLAQESGL
                     V"
     misc_feature    1875..1880
                     /label=SDS
     misc_feature    2113..2118
                     /label=SDS
     misc_feature    2965..2970
                     /label=SDS
     misc_feature    3181..3186
                     /label=SDS
     CDS             3750..3815
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3819..4250
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3963..3968
                     /label=SDS
     repeat_region   4293..4433
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4293..4422
                     /label=AAV2 ITR
     rep_origin      4508..4963
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5245..5349
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5350..6210
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6381..6969
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca gggcatgaat ctctccggag
     1501 gccagaagca gcggctgagc ctggcccggg ctgtatacag aaaggcagct gtgtacctgc
     1561 tggatgaccc cctggcggcc ctggatgccc acgttggcca gcatgtcttc aaccaggtca
     1621 ttgggcctgg tgggctactc cagggaacaa cacggattct cgtgacgcac gcactccaca
     1681 tcctgcccca ggctgattgg atcatagtgc tggcaaatgg ggccatcgca gagatgggtt
     1741 cctaccagga gcttctgcag aggaaggggg ccctcatgtg tcttctggat caagccagac
     1801 agccaggaga tagaggagaa ggagaaacag aacctgggac cagcaccaag gaccccagag
     1861 gcacctctgc aggcaggagg cccgagctta gacgcgagag gtccatcaag tcagtccctg
     1921 agaaggaccg taccacttca gaagcccaga cagaggttcc tctggatgac cctgacaggg
     1981 caggatggcc agcaggaaag gacagcatcc aatacggcag ggtgaaggcc acagtgcacc
     2041 tggcctacct gcgtgccgtg ggcacccccc tctgcctcta cgcactcttc ctcttcctct
     2101 gccagcaagt ggcctccttc tgccggggct actggctgag cctgtgggcg gacgaccctg
     2161 cagtaggtgg gcagcagacg caggcagccc tgcgtggcgg gatcttcggg ctcctcggct
     2221 gtctccaagc cattgggctg tttgcctcca tggctgcggt gctcctaggt ggggcccggg
     2281 catccaggtt gctcttccag aggctcctgt gggatgtggt gcgatctccc atcagcttct
     2341 ttgagcggac acccattggt cacctgctaa accgcttctc caaggagaca gacacggttg
     2401 acgtggacat tccagacaaa ctccggtccc tgctgatgta cgcctttgga ctcctggagg
     2461 tcagcctggt ggtggcagtg gctaccccac tggccactgt ggccatcctg ccactgtttc
     2521 tcctctacgc tgggtttcag agcctgtatg tggttagctc atgccagctg agacgcttgg
     2581 agtcagccag ctactcgtct gtctgctccc acatggctga gacgttccag ggcagcacag
     2641 tggtccgggc attccgaacc caggccccct ttgtggctca gaacaatgct cgcgtagatg
     2701 aaagccagag gatcagtttc ccgcgactgg tggctgacag gtggcttgcg gccaatgtgg
     2761 agctcctggg gaatggcctg gtgtttgcag ctgccacgtg tgctgtgctg agcaaagccc
     2821 acctcagtgc tggcctcgtg ggcttctctg tctctgctgc cctccaggtg acccagacac
     2881 tgcagtgggt tgttcgcaac tggacagacc tagagaacag catcgtgtca gtggagcgga
     2941 tgcaggacta tgcctggacg cccaaggagg ctccctggag gctgcccaca tgtgcagctc
     3001 agcccccctg gcctcagggc gggcagatcg agttccggga ctttgggcta agataccgac
     3061 ctgagctccc gctggctgtg cagggcgtgt ccttcaagat ccacgcagga gagaaggtgg
     3121 gcatcgttgg caggaccggg gcagggaagt cctccctggc cagtgggctg ctgcggctcc
     3181 aggaggcagc tgagggtggg atctggatcg acggggtccc cattgcccac gtggggctgc
     3241 acacactgcg ctccaggatc agcatcatcc cccaggaccc catcctgttc cctggctctc
     3301 tgcggatgaa cctcgacctg ctgcaggagc actcggacga ggctatctgg gcagccctgg
     3361 agacggtgca gctcaaagcc ttggtggcca gcctgcccgg ccagctgcag tacaagtgtg
     3421 ctgaccgagg cgaggacctg agcgtgggcc agaaacagct cctgtgtctg gcacgtgccc
     3481 ttctccggaa gacccagatc ctcatcctgg acgaggctac tgctgccgtg gaccctggca
     3541 cggagctgca gatgcaggcc atgctcggga gctggtttgc acagtgcact gtgctgctca
     3601 ttgcccaccg cctgcgctcc gtgatggact gtgcccgggt tctggtcatg gacaaggggc
     3661 aggtggcaga gagcggcagc ccggcccagc tgctggccca gaagggcctg ttttacagac
     3721 tggcccagga gtcaggcctg gtctgtacag actacaaaga ccatgacggt gattataaag
     3781 atcatgacat cgactacaag gatgacgatg acaagtaact cgagataatc aacctctgga
     3841 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     3901 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     3961 ctcctccttg tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct
     4021 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgtttatttg
     4081 tgaaatttgt gatgctattg ctttatttgt aaccatctag ctttatttgt gaaatttgtg
     4141 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     4201 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa agatctggag
     4261 accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac
     4321 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     4381 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
     4441 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
     4501 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4561 gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt
     4621 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4681 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     4741 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4801 aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt
     4861 gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa
     4921 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     4981 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     5041 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     5101 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     5161 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     5221 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5281 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5341 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5401 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5461 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5521 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5581 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5641 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5701 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5761 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5821 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5881 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5941 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6001 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6061 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6121 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6181 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6241 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6301 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6361 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6421 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6481 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6541 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6601 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6661 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6721 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6781 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6841 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6901 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6961 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7021 gctcacatgt
//