| 质粒名称: | pCMV-PE1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 132774 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | PE1,Cas9(H840A)-M-MLVrt |
| 物种来源: | Synthetic; Cas9 is from S. pyogenes; M-MLV RT is from the Moloney murine leukemia virus |
| 插入大小: | 6354 bp |
| 基因突变: | See manuscript |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: |
SV40 NLS（主干上的N终端）
SV40 NLS（主干上的C终端）
|
| 克隆方法: | Ligation Independent Cloning |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | T7-forward |
| 3' 测序引物 | BGH-reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-PE1 was a gift from David Liu (Addgene plasmid # 132774 ; http://n2t.net/addgene:132774 ; RRID:Addgene_132774) |
| 参考文献部分: | Search-and-replace genome editing without double-strand breaks or donor DNA. Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR. Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 10.1038/s41586-019-1711-4. 10.1038/s41586-019-1711-4 PubMed 31634902 |
买家导航
pCMVPE1载体基本信息
pCMVPE1载体图谱质粒图谱和多克隆位点信息
pCMVPE1载体简介
pCMVPE1载体序列
LOCUS pCMV-PE1 9753 bp ds-DNA circular SYN 18-OCT-2019
DEFINITION Prime editing in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCMV-PE1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9753)
TITLE Liu Lab plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9753)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Oct 18, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9753
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..204
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 155..175
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 246..265
/label=T7
/note="T7 promoter, forward primer"
promoter 246..264
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 314..334
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 335..4435
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(H840A)
/note="generates RNA-guided single strand nicks in DNA due
to the H840A mutation in the HNH catalytic domain"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 6608..6628
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6637..6654
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(6677..6694)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6683..6907
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(6978..6994)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6978..6994)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6991..7013)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7002..7018
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7026..7056)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7071..7092
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7209..7226)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7380..7968)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7460..7479)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8139..8999)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8762..8781
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9000..9104)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9183..9202)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 9374..9753
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg
241 gccgctaata cgactcacta tagggagagc cgccaccatg aaacggacag ccgacggaag
301 cgagttcgag tcaccaaaga agaagcggaa agtcgacaag aagtacagca tcggcctgga
361 catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa
421 gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc
481 cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag
541 aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat
601 ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga
661 taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga
721 gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga
781 cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat
841 cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca
901 gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc
961 catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc
1021 cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc
1081 caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac
1141 ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt
1201 tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac
1261 cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca
1321 ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat
1381 tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga
1441 agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct
1501 cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat
1561 cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta
1621 cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta
1681 ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga
1741 ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag
1801 cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa
1861 gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt
1921 gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga
1981 cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa
2041 gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc
2101 cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga
2161 ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga
2221 gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca
2281 gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat
2341 ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa
2401 cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa
2461 agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag
2521 ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt
2581 gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac
2641 ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga
2701 gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa
2761 gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat
2821 caaccggctg tccgactacg atgtggacgc tatcgtgcct cagagctttc tgaaggacga
2881 ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt
2941 gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa
3001 gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga
3061 actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca
3121 cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat
3181 ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt
3241 ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa
3301 cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta
3361 cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg
3421 caaggctacc gccaagtact tcttctacag caacatcatg aactttttca agaccgagat
3481 taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg
3541 ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc
3601 ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat
3661 cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa
3721 gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga
3781 aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga
3841 aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt
3901 gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg
3961 gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc
4021 caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga
4081 ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat
4141 cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt
4201 gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat
4261 ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac
4321 catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca
4381 gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag gtgactctgg
4441 aggatctagc ggaggatcct ctggcagcga gacaccagga acaagcgagt cagcaacacc
4501 agagagcagt ggcggcagca gcggcggcag cagcacccta aatatagaag atgagtatcg
4561 gctacatgag acctcaaaag agccagatgt ttctctaggg tccacatggc tgtctgattt
4621 tcctcaggcc tgggcggaaa ccgggggcat gggactggca gttcgccaag ctcctctgat
4681 catacctctg aaagcaacct ctacccccgt gtccataaaa caatacccca tgtcacaaga
4741 agccagactg gggatcaagc cccacataca gagactgttg gaccagggaa tactggtacc
4801 ctgccagtcc ccctggaaca cgcccctgct acccgttaag aaaccaggga ctaatgatta
4861 taggcctgtc caggatctga gagaagtcaa caagcgggtg gaagacatcc accccaccgt
4921 gcccaaccct tacaacctct tgagcgggct cccaccgtcc caccagtggt acactgtgct
4981 tgatttaaag gatgcctttt tctgcctgag actccacccc accagtcagc ctctcttcgc
5041 ctttgagtgg agagatccag agatgggaat ctcaggacaa ttgacctgga ccagactccc
5101 acagggtttc aaaaacagtc ccaccctgtt tgatgaggca ctgcacagag acctagcaga
5161 cttccggatc cagcacccag acttgatcct gctacagtac gtggatgact tactgctggc
5221 cgccacttct gagctagact gccaacaagg tactcgggcc ctgttacaaa ccctagggaa
5281 cctcgggtat cgggcctcgg ccaagaaagc ccaaatttgc cagaaacagg tcaagtatct
5341 ggggtatctt ctaaaagagg gtcagagatg gctgactgag gccagaaaag agactgtgat
5401 ggggcagcct actccgaaga cccctcgaca actaagggag ttcctaggga cggcaggctt
5461 ctgtcgcctc tggatccctg ggtttgcaga aatggcagcc cccctgtacc ctctcaccaa
5521 aacggggact ctgtttaatt ggggcccaga ccaacaaaag gcctatcaag aaatcaagca
5581 agctcttcta actgccccag ccctggggtt gccagatttg actaagccct ttgaactctt
5641 tgtcgacgag aagcagggct acgccaaagg tgtcctaacg caaaaactgg gaccttggcg
5701 tcggccggtg gcctacctgt ccaaaaagct agacccagta gcagctgggt ggcccccttg
5761 cctacggatg gtagcagcca ttgccgtact gacaaaggat gcaggcaagc taaccatggg
5821 acagccacta gtcattctgg ccccccatgc agtagaggca ctagtcaaac aaccccccga
5881 ccgctggctt tccaacgccc ggatgactca ctatcaggcc ttgcttttgg acacggaccg
5941 ggtccagttc ggaccggtgg tagccctgaa cccggctacg ctgctcccac tgcctgagga
6001 agggctgcaa cacaactgcc ttgatatcct ggccgaagcc cacggaaccc gacccgacct
6061 aacggaccag ccgctcccag acgccgacca cacctggtac acggatggaa gcagtctctt
6121 acaagaggga cagcgtaagg cgggagctgc ggtgaccacc gagaccgagg taatctgggc
6181 taaagccctg ccagccggga catccgctca gcgggctgaa ctgatagcac tcacccaggc
6241 cctaaagatg gcagaaggta agaagctaaa tgtttatact gatagccgtt atgcttttgc
6301 tactgcccat atccatggag aaatatacag aaggcgtggg ttgctcacat cagaaggcaa
6361 agagatcaaa aataaagacg agatcttggc cctactaaaa gccctctttc tgcccaaaag
6421 acttagcata atccattgtc caggacatca aaagggacac agcgccgagg ctagaggcaa
6481 ccggatggct gaccaagcgg cccgaaaggc agccatcaca gagactccag acacctctac
6541 cctcctcata gaaaattcat caccctctgg cggctcaaaa agaaccgccg acggcagcga
6601 attcgagccc aagaagaaga ggaaagtcta accggtcatc atcaccatca ccattgagtt
6661 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
6721 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
6781 gagaaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
6841 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
6901 tctatggctt ctgaggcgga aagaaccagc tggggctcga taccgtcgac ctctagctag
6961 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
7021 ccacacaaca tacgagccgg aagcataaag tgtaaagcct agggtgccta atgagtgagc
7081 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
7141 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
7201 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
7261 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
7321 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
7381 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
7441 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
7501 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
7561 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
7621 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
7681 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
7741 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
7801 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
7861 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
7921 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
7981 atcttttcta cggggtctga cactcagtgg aacgaaaact cacgttaagg gattttggtc
8041 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
8101 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
8161 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
8221 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
8281 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
8341 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
8401 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
8461 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
8521 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
8581 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
8641 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
8701 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
8761 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
8821 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
8881 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
8941 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
9001 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
9061 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
9121 gtgccacctg acgtcgacgg atcgggagat cgatctcccg atcccctagg gtcgactctc
9181 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
9241 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
9301 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
9361 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
9421 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
9481 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
9541 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
9601 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
9661 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
9721 tacatctacg tattagtcat cgctattacc atg
//
