pcambia2300

价格:1500元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: CAMBIA
载体名称: pCAMBIA2300
质粒类型: 植物载体;农杆菌双元表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: Lac
载体大小: 8743 bp
5' 测序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
3' 测序引物及序列: M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
载体标签: --
载体抗性: 卡那霉素
筛选标记: 新霉素(Neomycin)
克隆菌株: HB101等菌株
宿主细胞(系): 植物细胞、农杆菌
备注: --
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCAMBIA2300载体图谱



pCAMBIA2300 多克隆位点

载体简介

载体序列

LOCUS       pCAMBIA2300	8743 bp 	DNA	SYN
DEFINITION  pCAMBIA2300
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="pCAMBIA2300"
                     /mol_type="other DNA"
     CDS             234..920
                     /label="ORF frame 3"
     CDS             1219..1848
                     /label="ORF frame 1"
     CDS             complement(2145..2717)
                     /label="ORF frame 3"
     CDS             2175..3350
                     /label="ORF frame 3"
     misc_feature    complement(3846..3868)
                     /label="pGEX_3_primer"
     gene            complement(4955..5749)
                     /label="KanR1"
                     /gene="KanR1"
     CDS             complement(4955..5749)
                     /label="ORF frame 1"
     CDS             complement(6507..7445)
                     /label="ORF frame 3"
     CDS             complement(7798..8256)
                     /label="ORF frame 2"
     promoter        8271..8300
                     /label="lac_promoter"
     misc_feature    8314..8336
                     /label="M13_pUC_rev_primer"
     promoter        8335..8353
                     /label="M13_reverse_primer"
     promoter        complement(8419..8435)
                     /label="M13_forward20_primer"
     misc_feature    complement(8428..8450)
                     /label="M13_pUC_fwd_primer"                    
     misc_feature    8428..8571
                     /label="lacZ_a"                    
ORIGIN
    1 CATGCCAACC ACAGGGTTCC CCTCGGGATC AAAGTACTTT GATCCAACCC CTCCGCTGCT
   61 ATAGTGCAGT CGGCTTCTGA CGTTCAGTGC AGCCGTCTTC TGAAAACGAC ATGTCGCACA
  121 AGTCCTAAGT TACGCGACAG GCTGCCGCCC TGCCCTTTTC CTGGCGTTTT CTTGTCGCGT
  181 GTTTTAGTCG CATAAAGTAG AATACTTGCG ACTAGAACCG GAGACATTAC GCCATGAACA
  241 AGAGCGCCGC CGCTGGCCTG CTGGGCTATG CCCGCGTCAG CACCGACGAC CAGGACTTGA
  301 CCAACCAACG GGCCGAACTG CACGCGGCCG GCTGCACCAA GCTGTTTTCC GAGAAGATCA
  361 CCGGCACCAG GCGCGACCGC CCGGAGCTGG CCAGGATGCT TGACCACCTA CGCCCTGGCG
  421 ACGTTGTGAC AGTGACCAGG CTAGACCGCC TGGCCCGCAG CACCCGCGAC CTACTGGACA
  481 TTGCCGAGCG CATCCAGGAG GCCGGCGCGG GCCTGCGTAG CCTGGCAGAG CCGTGGGCCG
  541 ACACCACCAC GCCGGCCGGC CGCATGGTGT TGACCGTGTT CGCCGGCATT GCCGAGTTCG
  601 AGCGTTCCCT AATCATCGAC CGCACCCGGA GCGGGCGCGA GGCCGCCAAG GCCCGAGGCG
  661 TGAAGTTTGG CCCCCGCCCT ACCCTCACCC CGGCACAGAT CGCGCACGCC CGCGAGCTGA
  721 TCGACCAGGA AGGCCGCACC GTGAAAGAGG CGGCTGCACT GCTTGGCGTG CATCGCTCGA
  781 CCCTGTACCG CGCACTTGAG CGCAGCGAGG AAGTGACGCC CACCGAGGCC AGGCGGCGCG
  841 GTGCCTTCCG TGAGGACGCA TTGACCGAGG CCGACGCCCT GGCGGCCGCC GAGAATGAAC
  901 GCCAAGAGGA ACAAGCATGA AACCGCACCA GGACGGCCAG GACGAACCGT TTTTCATTAC
  961 CGAAGAGATC GAGGCGGAGA TGATCGCGGC CGGGTACGTG TTCGAGCCGC CCGCGCACGT
 1021 CTCAACCGTG CGGCTGCATG AAATCCTGGC CGGTTTGTCT GATGCCAAGC TGGCGGCCTG
 1081 GCCGGCCAGC TTGGCCGCTG AAGAAACCGA GCGCCGCCGT CTAAAAAGGT GATGTGTATT
 1141 TGAGTAAAAC AGCTTGCGTC ATGCGGTCGC TGCGTATATG ATGCGATGAG TAAATAAACA
 1201 AATACGCAAG GGGAACGCAT GAAGGTTATC GCTGTACTTA ACCAGAAAGG CGGGTCAGGC
 1261 AAGACGACCA TCGCAACCCA TCTAGCCCGC GCCCTGCAAC TCGCCGGGGC CGATGTTCTG
 1321 TTAGTCGATT CCGATCCCCA GGGCAGTGCC CGCGATTGGG CGGCCGTGCG GGAAGATCAA
 1381 CCGCTAACCG TTGTCGGCAT CGACCGCCCG ACGATTGACC GCGACGTGAA GGCCATCGGC
 1441 CGGCGCGACT TCGTAGTGAT CGACGGAGCG CCCCAGGCGG CGGACTTGGC TGTGTCCGCG
 1501 ATCAAGGCAG CCGACTTCGT GCTGATTCCG GTGCAGCCAA GCCCTTACGA CATATGGGCC
 1561 ACCGCCGACC TGGTGGAGCT GGTTAAGCAG CGCATTGAGG TCACGGATGG AAGGCTACAA
 1621 GCGGCCTTTG TCGTGTCGCG GGCGATCAAA GGCACGCGCA TCGGCGGTGA GGTTGCCGAG
 1681 GCGCTGGCCG GGTACGAGCT GCCCATTCTT GAGTCCCGTA TCACGCAGCG CGTGAGCTAC
 1741 CCAGGCACTG CCGCCGCCGG CACAACCGTT CTTGAATCAG AACCCGAGGG CGACGCTGCC
 1801 CGCGAGGTCC AGGCGCTGGC CGCTGAAATT AAATCAAAAC TCATTTGAGT TAATGAGGTA
 1861 AAGAGAAAAT GAGCAAAAGC ACAAACACGC TAAGTGCCGG CCGTCCGAGC GCACGCAGCA
 1921 GCAAGGCTGC AACGTTGGCC AGCCTGGCAG ACACGCCAGC CATGAAGCGG GTCAACTTTC
 1981 AGTTGCCGGC GGAGGATCAC ACCAAGCTGA AGATGTACGC GGTACGCCAA GGCAAGACCA
 2041 TTACCGAGCT GCTATCTGAA TACATCGCGC AGCTACCAGA GTAAATGAGC AAATGAATAA
 2101 ATGAGTAGAT GAATTTTAGC GGCTAAAGGA GGCGGCATGG AAAATCAAGA ACAACCAGGC
 2161 ACCGACGCCG TGGAATGCCC CATGTGTGGA GGAACGGGCG GTTGGCCAGG CGTAAGCGGC
 2221 TGGGTTGTCT GCCGGCCCTG CAATGGCACT GGAACCCCCA AGCCCGAGGA ATCGGCGTGA
 2281 GCGGTCGCAA ACCATCCGGC CCGGTACAAA TCGGCGCGGC GCTGGGTGAT GACCTGGTGG
 2341 AGAAGTTGAA GGCCGCGCAG GCCGCCCAGC GGCAACGCAT CGAGGCAGAA GCACGCCCCG
 2401 GTGAATCGTG GCAAGCGGCC GCTGATCGAA TCCGCAAAGA ATCCCGGCAA CCGCCGGCAG
 2461 CCGGTGCGCC GTCGATTAGG AAGCCGCCCA AGGGCGACGA GCAACCAGAT TTTTTCGTTC
 2521 CGATGCTCTA TGACGTGGGC ACCCGCGATA GTCGCAGCAT CATGGACGTG GCCGTTTTCC
 2581 GTCTGTCGAA GCGTGACCGA CGAGCTGGCG AGGTGATCCG CTACGAGCTT CCAGACGGGC
 2641 ACGTAGAGGT TTCCGCAGGG CCGGCCGGCA TGGCCAGTGT GTGGGATTAC GACCTGGTAC
 2701 TGATGGCGGT TTCCCATCTA ACCGAATCCA TGAACCGATA CCGGGAAGGG AAGGGAGACA
 2761 AGCCCGGCCG CGTGTTCCGT CCACACGTTG CGGACGTACT CAAGTTCTGC CGGCGAGCCG
 2821 ATGGCGGAAA GCAGAAAGAC GACCTGGTAG AAACCTGCAT TCGGTTAAAC ACCACGCACG
 2881 TTGCCATGCA GCGTACGAAG AAGGCCAAGA ACGGCCGCCT GGTGACGGTA TCCGAGGGTG
 2941 AAGCCTTGAT TAGCCGCTAC AAGATCGTAA AGAGCGAAAC CGGGCGGCCG GAGTACATCG
 3001 AGATCGAGCT AGCTGATTGG ATGTACCGCG AGATCACAGA AGGCAAGAAC CCGGACGTGC
 3061 TGACGGTTCA CCCCGATTAC TTTTTGATCG ATCCCGGCAT CGGCCGTTTT CTCTACCGCC
 3121 TGGCACGCCG CGCCGCAGGC AAGGCAGAAG CCAGATGGTT GTTCAAGACG ATCTACGAAC
 3181 GCAGTGGCAG CGCCGGAGAG TTCAAGAAGT TCTGTTTCAC CGTGCGCAAG CTGATCGGGT
 3241 CAAATGACCT GCCGGAGTAC GATTTGAAGG AGGAGGCGGG GCAGGCTGGC CCGATCCTAG
 3301 TCATGCGCTA CCGCAACCTG ATCGAGGGCG AAGCATCCGC CGGTTCCTAA TGTACGGAGC
 3361 AGATGCTAGG GCAAATTGCC CTAGCAGGGG AAAAAGGTCG AAAAGGTCTC TTTCCTGTGG
 3421 ATAGCACGTA CATTGGGAAC CCAAAGCCGT ACATTGGGAA CCGGAACCCG TACATTGGGA
 3481 ACCCAAAGCC GTACATTGGG AACCGGTCAC ACATGTAAGT GACTGATATA AAAGAGAAAA
 3541 AAGGCGATTT TTCCGCCTAA AACTCTTTAA AACTTATTAA AACTCTTAAA ACCCGCCTGG
 3601 CCTGTGCATA ACTGTCTGGC CAGCGCACAG CCGAAGAGCT GCAAAAAGCG CCTACCCTTC
 3661 GGTCGCTGCG CTCCCTACGC CCCGCCGCTT CGCGTCGGCC TATCGCGGCC GCTGGCCGCT
 3721 CAAAAATGGC TGGCCTACGG CCAGGCAATC TACCAGGGCG CGGACAAGCC GCGCCGTCGC
 3781 CACTCGACCG CCGGCGCCCA CATCAAGGCA CCCTGCCTCG CGCGTTTCGG TGATGACGGT
 3841 GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC
 3901 GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCGCAGCC
 3961 ATGACCCAGT CACGTAGCGA TAGCGGAGTG TATACTGGCT TAACTATGCG GCATCAGAGC
 4021 AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA
 4081 AATACCGCAT CAGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC
 4141 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG
 4201 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA
 4261 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
 4321 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
 4381 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
 4441 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
 4501 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
 4561 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
 4621 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
 4681 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
 4741 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
 4801 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
 4861 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
 4921 CACGTTAAGG GATTTTGGTC ATGCATTCTA GGTACTAAAA CAATTCATCC AGTAAAATAT
 4981 AATATTTTAT TTTCTCCCAA TCAGGCTTGA TCCCCAGTAA GTCAAAAAAT AGCTCGACAT
 5041 ACTGTTCTTC CCCGATATCC TCCCTGATCG ACCGGACGCA GAAGGCAATG TCATACCACT
 5101 TGTCCGCCCT GCCGCTTCTC CCAAGATCAA TAAAGCCACT TACTTTGCCA TCTTTCACAA
 5161 AGATGTTGCT GTCTCCCAGG TCGCCGTGGG AAAAGACAAG TTCCTCTTCG GGCTTTTCCG
 5221 TCTTTAAAAA ATCATACAGC TCGCGCGGAT CTTTAAATGG AGTGTCTTCT TCCCAGTTTT
 5281 CGCAATCCAC ATCGGCCAGA TCGTTATTCA GTAAGTAATC CAATTCGGCT AAGCGGCTGT
 5341 CTAAGCTATT CGTATAGGGA CAATCCGATA TGTCGATGGA GTGAAAGAGC CTGATGCACT
 5401 CCGCATACAG CTCGATAATC TTTTCAGGGC TTTGTTCATC TTCATACTCT TCCGAGCAAA
 5461 GGACGCCATC GGCCTCACTC ATGAGCAGAT TGCTCCAGCC ATCATGCCGT TCAAAGTGCA
 5521 GGACCTTTGG AACAGGCAGC TTTCCTTCCA GCCATAGCAT CATGTCCTTT TCCCGTTCCA
 5581 CATCATAGGT GGTCCCTTTA TACCGGCTGT CCGTCATTTT TAAATATAGG TTTTCATTTT
 5641 CTCCCACCAG CTTATATACC TTAGCAGGAG ACATTCCTTC CGTATCTTTT ACGCAGCGGT
 5701 ATTTTTCGAT CAGTTTTTTC AATTCCGGTG ATATTCTCAT TTTAGCCATT TATTATTTCC
 5761 TTCCTCTTTT CTACAGTATT TAAAGATACC CCAAGAAGCT AATTATAACA AGACGAACTC
 5821 CAATTCACTG TTCCTTGCAT TCTAAAACCT TAAATACCAG AAAACAGCTT TTTCAAAGTT
 5881 GTTTTCAAAG TTGGCGTATA ACATAGTATC GACGGAGCCG ATTTTGAAAC CGCGGTGATC
 5941 ACAGGCAGCA ACGCTCTGTC ATCGTTACAA TCAACATGCT ACCCTCCGCG AGATCATCCG
 6001 TGTTTCAAAC CCGGCAGCTT AGTTGCCGTT CTTCCGAATA GCATCGGTAA CATGAGCAAA
 6061 GTCTGCCGCC TTACAACGGC TCTCCCGCTG ACGCCGTCCC GGACTGATGG GCTGCCTGTA
 6121 TCGAGTGGTG ATTTTGTGCC GAGCTGCCGG TCGGGGAGCT GTTGGCTGGC TGGTGGCAGG
 6181 ATATATTGTG GTGTAAACAA ATTGACGCTT AGACAACTTA ATAACACATT GCGGACGTTT
 6241 TTAATGTACT GAATTAACGC CGAATTAATT CGGGGGATCT GGATTTTAGT ACTGGATTTT
 6301 GGTTTTAGGA ATTAGAAATT TTATTGATAG AAGTATTTTA CAAATACAAA TACATACTAA
 6361 GGGTTTCTTA TATGCTCAAC ACATGAGCGA AACCCTATAG GAACCCTAAT TCCCTTATCT
 6421 GGGAACTACT CACACATTAT TATGGAGAAA CTCGAGCTTG TCGATCGACT CTAGCTAGAG
 6481 GATCGATCCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG
 6541 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG
 6601 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGGTCCGCC
 6661 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC
 6721 GGCAAGCAGG CATCGCCATG TGTCACGACG AGATCCTCGC CGTCGGGCAT GCGCGCCTTG
 6781 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA
 6841 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG
 6901 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG
 6961 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC
 7021 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG
 7081 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCCT GGAGTTCATT CAGGGCACCG
 7141 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG
 7201 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA
 7261 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCC CCATGGTCGA TCGACAGATC
 7321 TGCGAAAGCT CGAGAGAGAT AGATTTGTAG AGAGAGACTG GTGATTTCAG CGTGTCCTCT
 7381 CCAAATGAAA TGAACTTCCT TATATAGAGG AAGGTCTTGC GAAGGATAGT GGGATTGTGC
 7441 GTCATCCCTT ACGTCAGTGG AGATATCACA TCAATCCACT TGCTTTGAAG ACGTGGTTGG
 7501 AACGTCTTCT TTTTCCACGA TGCTCCTCGT GGGTGGGGGT CCATCTTTGG GACCACTGTC
 7561 GGCAGAGGCA TCTTGAACGA TAGCCTTTCC TTTATCGCAA TGATGGCATT TGTAGGTGCC
 7621 ACCTTCCTTT TCTACTGTCC TTTTGATGAA GTGACAGATA GCTGGGCAAT GGAATCCGAG
 7681 GAGGTTTCCC GATATTACCC TTTGTTGAAA AGTCTCAATA GCCCTTTGGT CTTCTGAGAC
 7741 TGTATCTTTG ATATTCTTGG AGTAGACGAG AGTGTCGTGC TCCACCATGT TATCACATCA
 7801 ATCCACTTGC TTTGAAGACG TGGTTGGAAC GTCTTCTTTT TCCACGATGC TCCTCGTGGG
 7861 TGGGGGTCCA TCTTTGGGAC CACTGTCGGC AGAGGCATCT TGAACGATAG CCTTTCCTTT
 7921 ATCGCAATGA TGGCATTTGT AGGTGCCACC TTCCTTTTCT ACTGTCCTTT TGATGAAGTG
 7981 ACAGATAGCT GGGCAATGGA ATCCGAGGAG GTTTCCCGAT ATTACCCTTT GTTGAAAAGT
 8041 CTCAATAGCC CTTTGGTCTT CTGAGACTGT ATCTTTGATA TTCTTGGAGT AGACGAGAGT
 8101 GTCGTGCTCC ACCATGTTGG CAAGCTGCTC TAGCCAATAC GCAAACCGCC TCTCCCCGCG
 8161 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT
 8221 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT
 8281 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC
 8341 AGCTATGACC ATGATTACGA ATTCGAGCTC GGTACCCGGG GATCCTCTAG AGTCGACCTG
 8401 CAGGCATGCA AGCTTGGCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG
 8461 CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA
 8521 AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGCTAGAG
 8581 CAGCTTGAGC TTGGATCAGA TTGTCGTTTC CCGCCTTCAG TTTAAACTAT CAGTGTTTGA
 8641 CAGGATATAT TGGCGGGTAA ACCTAAGAGA AAAGAGCGTT TATTAGAATA ACGGATATTT
 8701 AAAAGGGCGT GAAAAGGTTT ATCCGTTCGT CCATTTGTAT GTG
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng
欢迎您的访问和咨询....