| 质粒名称: | ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 52985 |
| 存储实验室: | Robert Singer |
|
载体骨架基本信息 |
|
| 载体名称: | phage |
| 空载体大小: | 6200 bp |
| 完整质粒大小: | 8523 bp |
| 载体类型: | Lentiviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | ls-ha-MCP-VenusN-nls-ha-PCP-VenusC |
| 物种来源: | Synthetic |
| 插入大小: | 2252 bp |
| 启动子: | ubc |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | NotI(未破坏) |
| 3’克隆位点: | ClaI (未破坏) |
| 5’测序引物 | ubc (5'-CGC CGA TGA TTA TAT AAG GA-3') |
| 3’测序引物 | WPRE-R |
|
文献引用方法 |
|
| 材料和方法部分: | ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC was a gift from Robert Singer (Addgene plasmid # 52985 ; http://n2t.net/addgene:52985 ; RRID:Addgene_52985) |
| 参考文献部分: | Background free imaging of single mRNAs in live cells using split fluorescent proteins. Wu B, Chen J, Singer RH. Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038/srep03615. 10.1038/srep03615 PubMed 24402470 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC 8523 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION split Venus attached to MCP and PCP respectively.
ACCESSION .
VERSION .
KEYWORDS ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8523)
AUTHORS Wu B, Chen J, Singer RH
TITLE Background free imaging of single mRNAs in live cells using split
fluorescent proteins.
JOURNAL Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038/srep03615.
PUBMED 24402470
REFERENCE 2 (bases 1 to 8523)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8523
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 82..101
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 230..251
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 266..296
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 304..320
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 309..331
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 328..344
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 328..344
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 394..413
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 605..1238
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 1285..1410
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1907..2140
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2325..2369
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2662..2779
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2822..3220
/label=UbC promoter
/note="human ubiquitin C promoter"
regulatory 3223..3232
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3235..3255
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 3259..3285
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3286..3297
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
regulatory 3694..3703
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3700..4161
/codon_start=1
/product="improved N-terminal fragment of mVenus for use in
bimolecular fluorescence complementation (BiFC) (Kodama and
Hu, 2010)"
/label=VN155(I152L)
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIT"
primer_bind complement(3745..3766)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4006..4025)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
misc_feature 4174..4741
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 4191..4743
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4335..4352)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4562..4581
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4767..4787
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4791..4817
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 4818..4829
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
CDS 5226..5477
/codon_start=1
/product="C-terminal fragment of mVenus for use in
bimolecular fluorescence complementation (BiFC) (Kodama and
Hu, 2010)"
/label=VC155
/translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
primer_bind 5414..5435
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 5517..6105
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5570..5590)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5988..5999)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6180..6413
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(6532..6550)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6618..6722
/gene="bla"
/label=AmpR promoter
CDS 6723..7583
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6941..6960)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7754..8342
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8243..8262
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8496..8513
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
61 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
181 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
241 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
301 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
421 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
481 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
541 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
601 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
661 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
721 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
781 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
841 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
901 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
961 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
1021 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
1081 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
1141 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
1201 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
1261 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
1321 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
1381 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
1441 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
1501 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
1561 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
1621 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
1681 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
1741 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
1801 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
1861 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
1921 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
1981 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
2041 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
2101 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
2161 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
2221 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
2281 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
2341 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
2401 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
2461 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
2521 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
2581 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
2641 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
2701 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
2761 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
2821 tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct
2881 gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca
2941 gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta
3001 ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag
3061 gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga
3121 tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg
3181 tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg cggccgccat gggcccaaaa
3241 aagaaaagaa aagttggcta cccctacgac gtgcccgact acgccatcga aggccgccat
3301 atgctagccg ttaaaatggc ttctaacttt actcagttcg ttctcgtcga caatggcgga
3361 actggcgacg tgactgtcgc cccaagcaac ttcgctaacg ggatcgctga atggatcagc
3421 tctaactcgc gttcacaggc ttacaaagta acctgtagcg ttcgtcagag ctctgcgcag
3481 aatcgcaaat acaccatcaa agtcgaggtg cctaaaggcg cctggcgttc gtacttaaat
3541 atggaactaa ccattccaat tttcgccacg aattccgact gcgagcttat tgttaaggca
3601 atgcaaggtc tcctaaaaga tggaaacccg attccctcag caatcgcagc aaactccggc
3661 atctacgcgg attctagacg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag
3721 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
3781 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg
3841 atctgcacca ccggcaagct gcccgtgcct tggcccaccc tcgtgaccac cctgggctac
3901 ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
3961 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
4021 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
4081 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
4141 agccacaacg tctatatcac ctaaatcgat ggatccctcc ccccccccta acgttactgg
4201 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
4261 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
4321 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
4381 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
4441 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
4501 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
4561 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
4621 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
4681 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
4741 atatggccac agcctgcagg atgggcccaa aaaagaaaag aaaagttggc tacccctacg
4801 acgtgcccga ctacgccatc gaaggccgcc atctagcctc caaaaccatc gttctttcgg
4861 tcggcgaggc tactcgcact ctgactgaga tccagtccac cgcagaccgt cagatcttcg
4921 aagagaaggt cgggcctctg gtgggtcggc tgcgcctcac ggcttcgctc cgtcaaaacg
4981 gagccaagac cgcgtatcgc gtcaacctaa aactggatca ggcggacgtc gttgattccg
5041 gacttccgaa agtgcgctac actcaggtat ggtcgcacga cgtgacaatc gttgcgaata
5101 gcaccgaggc ctcgcgcaaa tcgttgtacg atttgaccaa gtccctcgtc gcgacctcgc
5161 aggtcgaaga tcttgtcgtc aaccttgtgc cgctgggccg tgcggatcca ccggtcgcca
5221 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
5281 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
5341 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
5401 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
5461 tggacgagct gtacaagtaa atcgatgacc tacgtcatgc aggggcgata gatcctaatc
5521 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
5581 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
5641 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
5701 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
5761 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
5821 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
5881 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
5941 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
6001 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
6061 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag
6121 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
6181 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
6241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
6301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
6361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
6421 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
6481 gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga
6541 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
6601 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
6661 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
6721 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
6781 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
6841 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
6901 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
6961 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
7021 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
7081 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
7141 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
7201 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
7261 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
7321 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
7381 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
7441 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
7501 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
7561 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
7621 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
7681 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
7741 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
7801 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
7861 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
7921 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
7981 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
8041 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
8101 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
8161 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
8221 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
8281 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
8341 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
8401 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
8461 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
8521 aag
//
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