ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC

LOCUS       ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC                8523 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  split Venus attached to MCP and PCP respectively.
ACCESSION   .
VERSION     .
KEYWORDS    ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8523)
  AUTHORS   Wu B, Chen J, Singer RH
  TITLE     Background free imaging of single mRNAs in live cells using split 
            fluorescent proteins.
  JOURNAL   Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038/srep03615.
  PUBMED    24402470
REFERENCE   2  (bases 1 to 8523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8523
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     82..101
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    230..251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        266..296
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    304..320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     309..331
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     328..344
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     328..344
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     394..413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             605..1238
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1285..1410
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1907..2140
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2325..2369
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2662..2779
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2822..3220
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      3223..3232
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3235..3255
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3259..3285
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3286..3297
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      3694..3703
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3700..4161
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIT"
     primer_bind     complement(3745..3766)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4006..4025)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    4174..4741
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4191..4743
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4335..4352)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4562..4581
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4767..4787
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4791..4817
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4818..4829
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5226..5477
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     5414..5435
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5517..6105
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5570..5590)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5988..5999)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6180..6413
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6532..6550)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6618..6722
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6723..7583
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6941..6960)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7754..8342
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8243..8262
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8496..8513
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
       61 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
      121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
      181 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      241 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      301 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
      361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
      421 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
      481 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
      541 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
      601 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
      661 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      721 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      781 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      841 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      901 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      961 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     1021 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     1081 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     1141 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     1201 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1261 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1321 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1381 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1441 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1501 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1561 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1621 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1681 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1741 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1801 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1861 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1921 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1981 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     2041 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     2101 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     2161 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     2221 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     2281 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     2341 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     2401 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2461 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2521 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2581 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     2641 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2701 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2761 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2821 tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct
     2881 gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca
     2941 gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta
     3001 ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag
     3061 gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga
     3121 tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg
     3181 tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg cggccgccat gggcccaaaa
     3241 aagaaaagaa aagttggcta cccctacgac gtgcccgact acgccatcga aggccgccat
     3301 atgctagccg ttaaaatggc ttctaacttt actcagttcg ttctcgtcga caatggcgga
     3361 actggcgacg tgactgtcgc cccaagcaac ttcgctaacg ggatcgctga atggatcagc
     3421 tctaactcgc gttcacaggc ttacaaagta acctgtagcg ttcgtcagag ctctgcgcag
     3481 aatcgcaaat acaccatcaa agtcgaggtg cctaaaggcg cctggcgttc gtacttaaat
     3541 atggaactaa ccattccaat tttcgccacg aattccgact gcgagcttat tgttaaggca
     3601 atgcaaggtc tcctaaaaga tggaaacccg attccctcag caatcgcagc aaactccggc
     3661 atctacgcgg attctagacg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag
     3721 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     3781 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg
     3841 atctgcacca ccggcaagct gcccgtgcct tggcccaccc tcgtgaccac cctgggctac
     3901 ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     3961 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     4021 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     4081 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     4141 agccacaacg tctatatcac ctaaatcgat ggatccctcc ccccccccta acgttactgg
     4201 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     4261 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     4321 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     4381 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     4441 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     4501 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     4561 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     4621 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     4681 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     4741 atatggccac agcctgcagg atgggcccaa aaaagaaaag aaaagttggc tacccctacg
     4801 acgtgcccga ctacgccatc gaaggccgcc atctagcctc caaaaccatc gttctttcgg
     4861 tcggcgaggc tactcgcact ctgactgaga tccagtccac cgcagaccgt cagatcttcg
     4921 aagagaaggt cgggcctctg gtgggtcggc tgcgcctcac ggcttcgctc cgtcaaaacg
     4981 gagccaagac cgcgtatcgc gtcaacctaa aactggatca ggcggacgtc gttgattccg
     5041 gacttccgaa agtgcgctac actcaggtat ggtcgcacga cgtgacaatc gttgcgaata
     5101 gcaccgaggc ctcgcgcaaa tcgttgtacg atttgaccaa gtccctcgtc gcgacctcgc
     5161 aggtcgaaga tcttgtcgtc aaccttgtgc cgctgggccg tgcggatcca ccggtcgcca
     5221 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
     5281 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     5341 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
     5401 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     5461 tggacgagct gtacaagtaa atcgatgacc tacgtcatgc aggggcgata gatcctaatc
     5521 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5581 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5641 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5701 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5761 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5821 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5881 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     5941 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6001 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     6061 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag
     6121 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     6181 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     6241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     6421 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     6481 gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga
     6541 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     6601 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6661 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6721 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6781 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6841 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6901 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6961 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7021 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7081 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7141 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7201 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7261 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7321 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7381 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7441 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7501 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7561 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7621 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7681 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7741 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7801 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7861 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7921 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7981 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8041 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8101 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8161 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8221 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8281 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8341 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8401 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8461 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8521 aag
//