| 质粒名称: | pBS-L1PA1-CH-mneo |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 51288 |
| 存储实验室: | Astrid Roy-Engel |
|
载体骨架基本信息 |
|
| 载体名称: | pBluescript II |
| 载体出品公司: | Stratagene |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | L1,L1RE1,Line1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | 含有反向新霉素抗性基因的mneoI指示盒（插入物上的C末端） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | T7 |
| 3' 测序引物 | SP6 |
|
文献引用方法 |
|
| 材料和方法部分: | pBS-L1PA1-CH-mneo was a gift from Astrid Roy-Engel (Addgene plasmid # 51288 ; http://n2t.net/addgene:51288 ; RRID:Addgene_51288) |
| 参考文献部分: | Evolutionary conservation of the functional modularity of primate and murine LINE-1 elements. Wagstaff BJ, Barnerssoi M, Roy-Engel AM. PLoS One. 2011 May 10;6(5):e19672. doi: 10.1371/journal.pone.0019672. 10.1371/journal.pone.0019672 PubMed 21572950 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBS-L1PA1-CH-mneo 11342 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION contains the fully codon optimized ORF1 and ORF2 of the human L1RP .
ACCESSION .
VERSION .
KEYWORDS pBS-L1PA1-CH-mneo
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11342)
AUTHORS Wagstaff BJ, Barnerssoi M, Roy-Engel AM
TITLE Evolutionary conservation of the functional modularity of primate
and murine LINE-1 elements.
JOURNAL PLoS One. 2011 May 10;6(5):e19672. doi:
10.1371/journal.pone.0019672.
PUBMED 21572950
REFERENCE 2 (bases 1 to 11342)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11342
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 4..459
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(91..110)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 301..322
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 585..607
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 599..616
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 626..645
/label=T7
/note="T7 promoter, forward primer"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
enhancer 935..1314
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1315..1518
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 1469..1489
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 1515..1539
/label=LNCX
/note="Human CMV promoter, forward primer"
primer_bind complement(6655..6674)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 8166..8185
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
promoter complement(8295..8624)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8309..8444
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(8363..8382)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 8609..8629
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
polyA_signal 8729..8863
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8753..8772)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 8807..8826
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(9154..9174)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(9154..9172)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(9193..9209)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9193..9209)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9206..9228)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9217..9233
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9241..9271)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9286..9307
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9424..9441)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9595..10183)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9675..9694)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10354..11214)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10977..10996
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11215..11319)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
661 gccccccctc gagagcacgc gttgccaagc tagtcgacca attctcatgt ttgacagctt
721 atcatcgcag atccgggcaa cgttgttgca ttgctgcagg cgcagaactg gtaggtatgg
781 aagatctata cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca
841 taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt
901 tatattggct catgtccaat atgaccgcca tgttgacatt gattattgac tagttattaa
961 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
1021 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
1081 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
1141 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc
1201 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
1261 cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
1321 cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
1381 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
1441 aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag
1501 gtctatataa gcagagctcg tttagtgaac cgtcagatct ctagaagctg ggtaccagct
1561 gctagctaaa accacaaaga tgggcaagaa gcagaaccgc aagaccggca acagcaagac
1621 ccagagcgcc agcccccccc ccaaggagcg cagcagcagc cccgccaccg agcagagctg
1681 gatggagaac gacttcgacg agctgcgcga ggagggcttc cgccgcagca actacagcga
1741 gctgcgcgag gacatccaga ccaagggcaa ggaggtggag aacttcgaga agaacctgga
1801 ggagtgcatc acccgcatca ccaacaccga gaagtgcctg aaggagctga tggagctgaa
1861 gaccaaggcc cgcgagctgc gcgaggagtg ccgcagcctg cgcagccgct gcgaccagct
1921 ggaggagcgc gtgagcgcaa tggaggacga gatgaacgag atgaagcgcg agggcaagtt
1981 ccgcgagaag cgcatcaagc gcaacgagca gagcctccag gagatatggg actacgtgaa
2041 gcgccccaac ctgcgcctga tcggcgtgcc cgagagcgac gtggagaacg gcaccaagct
2101 ggagaacacc ctacaggaca tcatccagga gaacttcccc aacctggccc gccaggccaa
2161 cgtgcagatc caggagatcc agcgcacccc ccagcgctac agcagccgcc gcgccacccc
2221 ccgccacatc atcgtgcgct tcaccaaggt ggagatgaag gagaagatgc tgcgcgccgc
2281 ccgcgagaag ggccgggtta ccctgaaggg caagcccatc cgcctgaccg ccgacctgag
2341 cgccgagacc ctacaggccc gccgcgagtg ggggccaatc ttcaacatcc tgaaggagaa
2401 gaacttccag ccccgcatca gctaccccgc caagctgagc ttcatcagcg agggcgagat
2461 caaatacttc atcgacaagc agatgctgcg cgacttcgtg accacccgcc ccgccctgaa
2521 ggagctgctg aaggaggccc tgaacatgga gcgcaacaac cggtatcagc ccctgcaaaa
2581 ccacgccaag atgtaaagac catcaagact aggaagaaac tggatccact aatgagcaaa
2641 atcaccagct aacgtctcaa tgaccggcag caccagccac atcaccatcc tgaccctgaa
2701 catcaacggc ctgaacagcg ccatcaagcg gcaccggctg gccagctgga tcaagagcca
2761 ggaccccagc gtgtgctgca tccaggagac ccacctgacc tgccgggaca cccaccggct
2821 gaagattaag ggctggcgga agatctacca ggccaacggc aagcagaaga aggccggcgt
2881 ggccatcctt gtcagcgaca agaccgactt caagcccacc aagatcaagc gggacaagga
2941 gggccactac atcatggtga agggcagcat ccagcaggag gagctgacca tcctgaacat
3001 ctacgccccc aacactggtg ccccccggtt catcaaacaa gttctgagcg accttcagcg
3061 ggacctggac agccacaccc tgatcatggg cgacttcaac acccccctga gcaccctgga
3121 ccggagcacc cggcagaagg tgaacaagga cacccaggag ctgaacagcg ccctgcacca
3181 ggccgacctg atcgacatct accggaccct gcaccccaag agcaccgagt acaccttctt
3241 cagcgccccc caccacacct acagcaagat cgaccacatc gtgggcagca aggccctgct
3301 gagcaagtgc aagcggactg agatcatcac caactacctg agcgaccaca gcgccatcaa
3361 gctggagctg cggatcaaga acctgaccca gagccggagc accacctgga agctgaacaa
3421 cctgctgctg aacgactact gggttcacaa cgagatgaag gccgagatca agatgttctt
3481 cgagaccaac gagaacaagg acaccaccta ccagaacctg tgggacgcct tcaaggccgt
3541 gtgccggggc aagttcatcg ccctgaacgc ctacaagcgg aagcaggagc ggagcaagat
3601 cgacaccctg accagccagc tgaaggagct ggagaagcag gagcagaccc acagcaaggc
3661 cagtcggcgg caggagatca ccaagatccg ggccgagctg aaggagatcg agacccagaa
3721 gacccttcag aagatcaacg agagccggag ctggttcttc gagcggatca acaagatcga
3781 ccggcccctg gcccggctga tcaagaagaa gcgggagaag aaccagatcg acaccatcaa
3841 gaacgacaag ggcgacatca ccaccgaccc caccgagatc cagaccacca tccgggagta
3901 ctacaagcac ctgtacgcca acaagctgga gaatctagag gagatggaca ccttcctgga
3961 cacctacacc ctgccccggc tgaaccagga ggaggtggag agcctgaacc ggcccatcac
4021 cggcagcgag atcgtggcca tcatcaacag cctgcccacc aagaagagcc ccggccccga
4081 cggcttcacc gccgagttct accagcggta caaggaggag ctggtgccct tcctgctgaa
4141 gctgttccag agcatcgaga aggagggcat cctgcccaac agcttctacg aggccagcat
4201 catcctgatc cccaagcccg gccgggacac caccaagaag gagaacttcc ggcccatcag
4261 cctgatgaac atcgacgcca agatcctgaa caagatcctg gccaaccgga ttcagcagca
4321 catcaagaag ctgatccacc acgaccaagt tggcttcatc cccggcatgc aaggatggtt
4381 caacatccgg aagagcatca acgtgatcca gcacatcaac cgggccaagg acaagaacca
4441 catgatcatc agcatcgacg ccgagaaggc tttcgacaag atccagcagc ccttcatgct
4501 gaagaccctg aacaagctgg gcatcgacgg cacctacttc aagatcatcc gggccatcta
4561 cgacaagccc accgccaaca tcatcctgaa cggccagaag ctggaggctt tccccctgaa
4621 gaccggcacc cggcagggct gccccctgag ccccctgctg ttcaacatcg tgctggaggt
4681 gctggcccgg gccatccggc aggagaagga gatcaagggc atccagctgg gcaaggagga
4741 ggtgaagctg agcctgttcg ccgacgacat gatcgtgtac ctggagaacc ctattgttag
4801 cgcccagaac ctgctgaagc tgatcagcaa cttcagtaaa gttagcggct acaagatcaa
4861 cgtgcagaag agccaggctt tcctgtacac caataaccgg cagaccgaga gccagatcat
4921 gggcgagctg cccttcacca tcgccagcaa gcggatcaag tacctgggca tccagctgac
4981 tcgggacgtc aaggacctgt tcaaggagaa ctacaagccc ctgctgaagg agatcaagga
5041 ggagaccaac aagtggaaga acatcccctg tagctgggtg ggccggatca acatcgtgaa
5101 gatggccatc ctgccgaaag ttatctatcg gttcaacgcc atccccatca agctgcccat
5161 gaccttcttc accgagctgg agaagaccac cctgaagttc atctggaacc agaagcgggc
5221 ccggatcgcc aagagcatcc tgagccagaa gaacaaggcc ggcggcatca ccctgcccga
5281 cttcaagctg tactacaagg ccaccgtgac caagaccgcc tggtactggt accagaaccg
5341 ggacatcgac cagtggaacc ggaccgagcc cagcgagatc atgccccaca tctacaacta
5401 cctgatcttc gacaagcccg agaagaacaa gcagtggggc aaggacagcc tgttcaacaa
5461 gtggtgctgg gagaactggc tggccatctg ccggaagctg aagctggacc ccttcctgac
5521 cccctacacc aagatcaaca gccggtggat caaggacctg aacgtgaagc ccaagaccat
5581 caagaccctg gaggagaacc tgggcatcac catccaggac atcggcgtgg gcaaggactt
5641 catgagcaag acccccaagg ccatggccac caaggacaag atcgacaagt gggacctgat
5701 caagctgaag agcttctgca ccgccaagga gaccaccatc cgggtgaacc ggcagcccac
5761 cacctgggag aagattttcg ccacctacag cagcgacaag ggcctgatca gccggatcta
5821 caacgagctg aagcagattt acaagaagaa gaccaacaac cccatcaaga agtgggccaa
5881 ggacatgaac cggcacttca gcaaggagga catctacgcc gccaagaagc acatgaagaa
5941 gtgcagcagc agcctggcca tccgggagat gcagatcaag accaccatgc ggtatcacct
6001 gacccccgtg cggatggcca tcatcaagaa gagcggcaac aaccgatgct ggcggggctg
6061 cggcgagatc ggcaccctgc tgcactgctg gtgggactgc aagctggtgc agcccctgtg
6121 gaagagcgtg tggcggttcc tgcgggacct ggagctggag atccccttcg accccgccat
6181 ccccctgctg ggcatctacc ccaacgagta caagagctgc tgctacaagg acacctgcac
6241 ccggatgttc atcgccgccc tgttcaccat cgccaagacc tggaaccagc ccaagtgccc
6301 caccatgatc gactggatca agaagatgtg gcacatctac actatggagt actacgccgc
6361 catcaagaac gacgagttca tcagcttcgt gggcacctgg atgaagctgg agaccatcat
6421 cctgagcaag ctgagccagg agcagaagac caagcaccgg atcttcagcc tgatcggcgg
6481 caactaagaa ttcctagctg gccggccgtg cgttttattc ctgtcttttt attgccgatc
6541 ccctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
6601 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
6661 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
6721 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt
6781 cacgacgaga tcatcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg
6841 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg
6901 agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc
6961 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
7021 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
7081 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
7141 ccgcgctgcc tcgtcctgaa ggtgagtcca ggagatgttt cagcactgtt gcctttagtc
7201 tcgaggcaac ttagacaact gagtattgat ctgagcacag cagggtgtga gctgtttgaa
7261 gatactgggg ttgggggtga agaaactgca gaggactaac tgggctgaga cccagtggca
7321 atgttttagg gcctaaggaa tgcctctgaa aatctagatg gacaactttg actttgagaa
7381 aagagaggtg gaaatgagga aaatgacttt tctttattag atttcggtag aaagaacttt
7441 cacctttccc ctatttttgt tattcgtttt aaaacatcta tctggaggca ggacaagtat
7501 ggtcgttaaa aagatgcagg cagaaggcat atattggctc agtcaaagtg ggggaacttt
7561 ggtggccaaa catacattgc taaggctatt cctatatcag ctggacacat ataaaatgct
7621 gctaatgctt cattacaaac ttatatcctt taattccaga tgggggcaaa gtatgtccag
7681 gggtgaggaa caattgaaac atttgggctg gagtagattt tgaaagtcag ctctgtgtgt
7741 gtgtgtgtgt gtgtgtgtgt gtgtgtgcgc gcacgtgtgt ttgtgtgtgt gtgagagcgt
7801 gtgtttcttt taacgttttc agcctacagc atacagggtt catggtggca agaagataac
7861 aagatttaaa ttatggccag tgactagtgc tgcaagaaga acaactacct gcatttaatg
7921 ggaaagcaaa atctcaggct ttgagggaag ttaacatagg cttgattctg ggtggaagct
7981 gggtgtgtag ttatctggag gccaggctgg agctctcagc tcactatggg ttcatcttta
8041 ttgtctcctt tcatctcaac agctcattca gggcaccgga caggtcggtc ttgacaaaaa
8101 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
8161 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
8221 atccatcttg ttcaatggcc gatcccatat tggctgcagg gtcgctcggt gttcgaggcc
8281 acacgcgtaa gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
8341 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
8401 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
8461 gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
8521 cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg
8581 cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca cagctgatcg
8641 ataccgtaag ccgaattggg caatgtgcac atgtacccta aaacttaaag tataataaag
8701 acgtcagggt tcgaaatcga taagcttgga tccagacatg ataagataca ttgatgagtt
8761 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
8821 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
8881 tcattttatg tttcaggttc agggggaggt ggggaggttt tttaaagcaa gtaaaacctc
8941 tacaaatgtg gtatggctga ttatgatccg gctagatccg atccgtcgac cgatgccctt
9001 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
9061 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctattgcg
9121 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
9181 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
9241 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
9301 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
9361 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
9421 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
9481 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
9541 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
9601 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
9661 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
9721 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
9781 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
9841 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
9901 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
9961 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
10021 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
10081 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
10141 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
10201 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
10261 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
10321 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
10381 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
10441 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
10501 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
10561 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
10621 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
10681 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
10741 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
10801 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
10861 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
10921 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
10981 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
11041 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
11101 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
11161 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
11221 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
11281 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
11341 ac
//
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