pCoPURO

LOCUS       pCoPURO	4041 bp 	DNA	SYN
DEFINITION  pCoPURO
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="pCoPURO"
                     /mol_type="other DNA"
     misc_feature    complement(29..51)
                     /label="pGEX_3_primer"
     misc_feature    complement(250..393)
                     /label="lacZ_a"
     misc_feature    364..386
                     /label="M13_pUC_fwd_primer"
     promoter        379..395
                     /label="M13_forward20_primer"
     CDS             complement(762..1544)
                     /label="ORF frame 2"
     gene            894..1493
                     /label="puro (variant)"
                     /gene="puro (variant)"
     CDS             894..1493
                     /label="ORF frame 3"
     misc_feature    complement(1627..1643)
                     /label="pBluescriptKS_primer"
     terminator      1661..1792
                     /label="SV40_PA_terminator"
     misc_feature    1749..1768
                     /label="EBV_rev_primer"
     misc_feature    complement(1833..1855)
                     /label="M13_pUC_rev_primer"
     promoter        complement(1869..1898)
                     /label="lac_promoter"
     rep_origin      complement(2207..2826)
                     /label="pBR322_origin"
     gene            complement(2981..3841)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(2981..3841)
                     /label="ORF frame 3"
     promoter        complement(3883..3911)
                     /label="AmpR_promoter"
ORIGIN
    1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
   61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
  121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
  181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
  241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
  301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
  361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgCAGGTCG
  421 ACTCTAGAGG ATCcggtgcg tggtggtttc atgcttctgg gaacggcaaa tgggtttagg
  481 attgggaacc cctcatcatC TGTTGGAATA TACTATTCAA CCTACAAAAA TAACGTTAAA
  541 CAACACTACT TTATATTTGA TATGAATGGC CACACCTTTT ATGCCATAAA ACATATTGTA
  601 AGAGAATACC ACTCTTTTTA TTCCTTCTTT CCTTCTTGTA CGTTTTTTGC TGTGAGTAGG
  661 TCGTGGTGCT GGTGTTGCAG TTGAAATAAC TTAAAATATA AATCATAAAA CTCAAACATA
  721 AACTTGACTA TTTATTTATT TATTAAgaaa ggaaatataa attataaatt acaacagatc
  781 ccccgggctg caggaattcg atagcttgca tgcctgcagg tcggccgcca cgaccggtgc
  841 cgccaccatc ccctgaccca cgcccctgac ccctcacaag gagacgacct tccATGACCG
  901 AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCCGGGCC GTACGCACCC
  961 TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGACCCG GACCGCCACA
 1021 TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA
 1081 AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG
 1141 AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC
 1201 TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT
 1261 GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG
 1321 TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT
 1381 CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG
 1441 AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGAcgcccgc
 1501 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatggctcc gaccgaagcc
 1561 acccggggcg gccccgccga ccccgcaccc gcccccgagg cccaccgact ctagatcaag
 1621 cttatcgata ccgtcgacct cgaggccagc tagcttgaac TTGTTTATTG CAGCTTATAA
 1681 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA
 1741 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA TCGGGTaccg
 1801 agctcgaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
 1861 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
 1921 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
 1981 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
 2041 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
 2101 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
 2161 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
 2221 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
 2281 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
 2341 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
 2401 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
 2461 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
 2521 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
 2581 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
 2641 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
 2701 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
 2761 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
 2821 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
 2881 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
 2941 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
 3001 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
 3061 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
 3121 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
 3181 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
 3241 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
 3301 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
 3361 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
 3421 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
 3481 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
 3541 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
 3601 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
 3661 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
 3721 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
 3781 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
 3841 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
 3901 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
 3961 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
 4021 gtatcacgag gccctttcgt c
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