| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pcDNA3.1/Myc-His B , pcDNA3.1/Myc-HisB |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 启动子: | CMV |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5498 bp |
| 5' 测序引物: | pcDNA3.1-F:CTAGAGAACCCACTGCTTAC |
| 3' 测序引物: | pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG |
| 载体标签: | C-His, C-Myc |
| 载体抗性: | Ampicillin 氨苄 |
| 筛选标记: | Neomycin (G418) |
| 备注: |
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| 产品目录号: | V855-20 |
| 稳定性: | 瞬表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
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pcDNA3.1/Myc-HisB载体基本信息
pcDNA3.1/Myc-HisB载体图谱质粒图谱和多克隆位点信息
pcDNA3.1/Myc-HisB载体简介
pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice.
pcDNA3.1/Myc-HisB载体序列
LOCUS pcDNA3.1(+)/myc-His B 5498 bp DNA circular SYN 2-SEP-2003 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers source 1..5498 /invitrogen="127" /vntifkey="98" CDS 1046..1063 /invitrogen="1010000" /vntifkey="4" /label=6xHis CDS complement(4502..5362) /invitrogen="1020000" /vntifkey="4" /label=Amp(R) polyA_site 1092..1316 /invitrogen="1870000" /vntifkey="26" /label=BGH\pA primer complement(1086..1103) /invitrogen="1910000" /vntifkey="27" /label=BGH\reverse\primer promoter complement(5363..5461) /invitrogen="2330000" /vntifkey="30" /label=bla\promoter primer 769..789 /invitrogen="1930000" /vntifkey="27" /label=CMV\forward\primer promoter 232..819 /invitrogen="2180000" /vntifkey="29" /label=CMV\promoter CDS 1001..1030 /invitrogen="1090000" /vntifkey="4" /label=c-myc\epitope rep_origin 1362..1790 /invitrogen="2500000" /vntifkey="33" /label=f1\origin misc_feature 902..1003 /invitrogen="1510000" /vntifkey="21" /label=MCS CDS 2201..2995 /invitrogen="1260000" /vntifkey="4" /label=Neo(R) rep_origin complement(3684..4357) /invitrogen="2540000" /vntifkey="33" /label=pUC\origin promoter 1795..2165 /invitrogen="2290000" /vntifkey="29" /label=SV40\early\promoter polyA_signal 3171..3301 /invitrogen="1840000" /vntifkey="25" /label=SV40\pA primer 863..882 /invitrogen="2100000" /vntifkey="27" /label=T7\primer promoter 863..879 /invitrogen="2370000" /vntifkey="30" /label=T7\promoter BASE COUNT 1273 a 1428 c 1403 g 1394 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca 961 gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaacaaaaac tcatctcaga 1021 agaggatctg aatatgcata ccggtcatca tcaccatcac cattgagttt aaacccgctg 1081 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 1141 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 1201 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 1261 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc 1321 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc 1381 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 1441 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 1501 tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga 1561 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 1621 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 1681 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tggggatttc 1741 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg 1801 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca 1861 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 1921 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 1981 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 2041 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg 2101 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat 2161 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 2221 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 2281 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 2341 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 2401 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 2461 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 2521 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 2581 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 2641 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 2701 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 2761 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 2821 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 2881 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 2941 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 3001 actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 3061 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 3121 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 3181 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 3241 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 3301 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 3361 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 3421 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 3481 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 3541 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 3601 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 3661 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 3721 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 3781 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 3841 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 3901 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca 3961 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4021 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4081 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 4141 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 4201 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 4261 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 4321 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 4381 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 4441 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 4501 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 4561 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 4621 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 4681 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 4741 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 4801 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 4861 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 4921 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 4981 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 5041 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 5101 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 5161 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 5221 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 5281 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 5341 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 5401 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 5461 ttccgcgcac atttccccga aaagtgccac ctgacgtc //
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pcDNA3.1/Myc-HisB载体质粒应用举例
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