pNTAP-A

价格:1500元

联系方式:I47-825O-882O

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pNTAPA载体基本信息

出品公司: Agilent Technologies
载体名称: pNTAP-A , pNTAPA
质粒类型: 哺乳细胞表达系统载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4530bp
5' 测序引物及序列: CMV-F: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: SV40pA-R: GAAATTTGTGATGCTATTGC
载体标签: N端-streptavidin结合多肽 ; N端-calmodulin结合肽段 
载体抗性: 卡那霉素
筛选标记: Neomycin_1.html' target='_blank'>新霉素
备注: 能够表达N端含有能结合链霉亲和素的多肽片段,以及N端结合钙调蛋白结合多肽片段的载体.
产品目录号: --
稳定性: 诱导表达
组成型: 非组成型
病毒/非病毒: 非病毒
streptavidin

pNTAPA载体质粒图谱和多克隆位点信息

pNTAP-A载体图谱

pNTAPA载体简介

pNTAPA载体序列

LOCUS       pNTAP-A           4530 bp ds-DNA    circular SYN 28-十一月-2013
DEFINITION  Vector for expressing proteins N-terminally tagged with calmodulin- 
            and streptavidin-binding peptides.?For other reading frames, use 
            pNTAP-B or pNTAP-C.
ACCESSION   .
VERSION     .
KEYWORDS    pNTAP-A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        66..370
                     /label="CMV enhancer"
                     /label="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label="CMV promoter"
                     /label="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        620..638
                     /label="T3 promoter"
                     /label="promoter for bacteriophage T3 RNA polymerase"
     CDS             679..681
                     /codon_start=1
                     /product="start codon"
                     /label="ATG"
                     /translation="M"
     CDS             682..759
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label="CBP"
                     /label="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             778..891
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label="SBP"
                     /label="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     misc_feature    913..986
                     /label="MCS"
                     /label="multiple cloning site"
     misc_feature    994..1004
                     /label="stop codons"
                     /label="stop codons in all three reading frames"
     promoter        complement(1032..1050)
                     /label="T7 promoter"
                     /label="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1324..1445
                     /label="SV40 poly(A) signal"
                     /label="SV40 polyadenylation signal"
     rep_origin      complement(1452..1907)
                     /direction=LEFT
                     /label="f1 ori"
                     /label="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1934..2036
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        2040..2397
                     /label="SV40 promoter"
                     /label="SV40 enhancer and early promoter"
     CDS             2432..3226
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label="NeoR/KanR"
                     /label="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    3458..3505
                     /label="HSV TK poly(A) signal"
                     /label="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      3834..4422
                     /direction=RIGHT
                     /label="ori"
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgccaccat gaagcgacga tggaaaaaga atttcatagc cgtctcagca
      721 gccaaccgct ttaagaaaat ctcatcctcc ggggcacttg gaagcggtag cggtaccatg
      781 gacgagaaga ccaccggctg gcggggcggc cacgtggtgg agggcctggc cggcgagctg
      841 gagcagctgc gggccaggct ggagcaccac cctcagggcc agcgggagcc ctccggcggc
      901 tgcaagctgg gcgcccgggc ggatcccccg ggctgcagga attcgatatc aagcttatcg
      961 ataccgtcga cctcgagggg gggcccggta ccttaattaa ttaaggtacc aggtaagtgt
     1021 acccaattcg ccctatagtg agtcgtatta caattcactc gatcgccctt cccaacagtt
     1081 gcgcagcctg aatggcgaat ggagatccaa tttttaagtg tataatgtgt taaactactg
     1141 attctaattg tttgtgtatt ttagattcac agtcccaagg ctcatttcag gcccctcagt
     1201 cctcacagtc tgttcatgat cataatcagc cataccacat ttgtagaggt tttacttgct
     1261 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     1321 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1381 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1441 tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1501 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     1561 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     1621 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     1681 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     1741 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     1801 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     1861 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     1921 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1981 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagaatcc
     2041 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     2101 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     2161 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2221 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     2281 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     2341 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     2401 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     2461 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     2521 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     2581 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     2641 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     2701 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     2761 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     2821 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     2881 cggtcttgtc gatcaggatg atctggacga agaacatcag gggctcgcgc cagccgaact
     2941 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     3001 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     3061 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     3121 agaacttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     3181 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     3241 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     3301 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     3361 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     3421 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     3481 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     3541 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     3601 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     3661 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     3721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3961 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     4021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4441 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4501 cctgattctg tggataaccg tattaccgcc
//

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