| 质粒名称: | pGM1190 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 69994 |
| 存储实验室: | Günther Muth |
|
载体骨架基本信息 |
|
| 载体名称: | pSG5 |
| 细菌耐药性: | 安普霉素 |
| 空载体大小: | 2140 bp |
| 完整质粒大小: | 6537 bp |
| 载体类型: | 细菌表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
| 宿生菌： | DH5alpha |
| 生长说明: | 也在链霉菌中复制（低于35°C） |
|
插入基因1信息 |
|
| 插入基因名称: | aac |
| 备用名称: | aac(3)IV |
| 物种来源: | 大肠杆菌 |
| 插入大小: | 803bp |
|
插入基因2信息 |
|
| 插入基因名称: | tsr |
| 备用名称: | thiostrepton resistance |
| 物种来源: | 蓝链霉菌Streptomyces azureus |
| 插入大小: | 809 bp |
| GenBank编号 | X02392 |
|
插入基因3信息 |
|
| 插入基因名称: | rep |
| 物种来源: | 加纳链霉菌Streptomyces ghanaensis |
| 插入大小: | 1442 bp |
|
插入基因4信息 |
|
| 插入基因名称: | sso |
| 备用名称: | single stranded origin |
| 物种来源: | 加纳链霉菌Streptomyces ghanaensis |
| 插入大小: | 300 bp |
|
插入基因5信息 |
|
| 插入基因名称: | TipA promoter |
| 备用名称: | P_tipA |
| 物种来源: | 变铅青链霉菌Streptomyces lividans |
| 插入大小: | 100 bp |
|
插入基因6信息 |
|
| 插入基因名称: | dso |
| 备用名称: |
double-stranded origin
|
|
文献引用方法 |
|
| 材料和方法部分: | pGM1190 was a gift from Günther Muth (Addgene plasmid # 69994 ; http://n2t.net/addgene:69994 ; RRID:Addgene_69994) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGM1190 6973 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION OriT containing mobilizable vector for thiostrepton induced gene
expression in Streptomyces.
ACCESSION .
VERSION .
KEYWORDS pGM1190
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6973)
TITLE pGM1202, a mobilizable vector for gene expression in Streptomyces
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6973)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6973
/organism="synthetic DNA construct"
/mol_type="other DNA"
oriT 467..576
/note="incP origin of transfer"
CDS 892..1692
/codon_start=1
/gene="aac(3)IV"
/product="aminoglycoside 3-N-acetyltransferase type IV
"
/label=ApmR
/note="confers resistance to apramycin (Paget and Davies,
1996)"
/translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
rep_origin 1994..2582
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2483..2502
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2736..2753
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2870..2891
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2906..2936
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2944..2960
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2949..2971
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2968..2984
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2968..2984
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
terminator 3163..3197
/label=lambda t0 terminator
/note="minimal transcription terminator from phage lambda
(Scholtissek and Grosse, 1987)"
misc_feature 3368..3424
/label=MCS
/note="pUC18/19 multiple cloning site"
terminator 3572..3620
/label=fd terminator
/note="central terminator from bacteriophage fd (Otsuka and
Kunisawa, 1982)"
CDS 5335..6777
/codon_start=1
/gene="rep"
/product="replication initiator protein from the
Streptomyces ghanaensis plasmid pSG5"
/label=pSG5 Rep
/translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
APVLQPWGRLPGDCLRVDTAVA"
ORIGIN
1 ggccgtcgac gacgacgcgt ccgcctgcct gcttttggcc ggcgggccgg gccgcggttg
61 tccgccgggt cggaccggtc ccttggtcga ccgatgcctc ggccacgacg atgctgggcc
121 cgaagcggta cgcgaacccg tgccgggctg cggcggatcg cggcagcagg agctccccgc
181 ccgccacggc tggctggatg gtcgcctcac gcgcccgcag gggtgacggc gtgggtgcgg
241 cggtcagccc cgtgatcggg cgtcagacgg ccgctgaggg accgcgacca cccctccctt
301 cccgcctgcc gtctttcacc gcccggccgg tccgcagacg ccgtacggcc aggccacgga
361 ggattacggg cagatggcgc ccacccggca cccgggctgg ggcaccctgg acagcacgaa
421 ggcccgcccc actcaccggg gcgggccttc gcgttgagct agagttcccg ccagcctcgc
481 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
541 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
601 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
661 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa tgcagctcac
721 ggtaactgat gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa
781 aatgccggcc tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt
841 atcaccaccg actatttgca acagtgccgt tgatcgtgct atgatcgact gatgtcatca
901 gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat cggtcagctt
961 ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc acagctcctt ccgtagcgtc
1021 cggcccctcg aagatgggcc acttggactg atcgaggccc tgcgtgctgc gctgggtccg
1081 ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt cgatcctgcc
1141 acgtcgcccg ttacaccgga ccttggagtt gtctctgaca cattctggcg cctgccaaat
1201 gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg ggccacaggc agagcagatc
1261 atctctgatc cattgcccct gccacctcac tcgcctgcaa gcccggtcgc ccgtgtccat
1321 gaactcgatg ggcaggtact tctcctcggc gtgggacacg atgccaacac gacgctgcat
1381 cttgccgagt tgatggcaaa ggttccctat ggggtgccga gacactgcac cattcttcag
1441 gatggcaagt tggtacgcgt cgattatctc gagaatgacc actgctgtga gcgctttgcc
1501 ttggcggaca ggtggctcaa ggagaagagc cttcagaagg aaggtccagt cggtcatgcc
1561 tttgctcggt tgatccgctc ccgcgacatt gtggcgacag ccctgggtca actgggccga
1621 gatccgttga tcttcctgca tccgccagag gcgggatgcg aagaatgcga tgccgctcgc
1681 cagtcgattg gctgagctca taagttccta ttccgaagtt cctattcgaa atgaccgacc
1741 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
1801 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
1861 tgctggagtt cttcgcccac cccaaaagga tctaggtgaa gatccttttt gataatctca
1921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
1981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
2041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
2101 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
2161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
2221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
2281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
2341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
2401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
2461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
2521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
2581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
2641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
2701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
2761 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
2821 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
2881 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
2941 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattgt
3001 acgtaggcgt cagctacgat gttccgggga ctgctgatcc ggtcagcagg tggaagaggg
3061 actggattcc aaagttctca atgctgcttg ctgttcttga atggggggtc gttgacgacg
3121 acatggctcg attggcgcga caagttgctg cgattctcac caataaaaaa cgcccggcgg
3181 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg accggatcgg
3241 ggatctgggc tgagggagcc gacggcacgc ggcggctcac ggcgtggcac gcggaacgtc
3301 cgggcttgca cctcacgtca cgtgaggagg cagcgtggac ggcgtcagag aagggagcgg
3361 acatatgaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta ccgagctcga
3421 attccccaga tctaaagttt tgtcgtcttt ccagacgtta gtaaatgaat tttctgtatg
3481 aggttttgct aaacaacttt caacagtttc agcggagtga gaatagaaag gaacaactaa
3541 aggaattgcg aataataatt ttttcacgtt gaaaatctcc aaaaaaaaag gctccaaaag
3601 gagcctttaa ttgtatcggt ttatcagctt gctttcgagg tgaatttctt aaacagcttg
3661 ataccgatag ttgcgccgac aatgacaaca accatcgccc acgcataacc gatatattcg
3721 gtcgctgagg cttgcaggga gtcaaaggcc gcttttgcgg gatctcgtcg aaggcactag
3781 aggcctatat gcggggatcg accgcgcggg tcccggacgg ggaagagcgg ggagctttgc
3841 cagagagcga cgacttcccc ttgcgttggt gattgccggt cagggcagcc atccgccatc
3901 gtcgcgtagg gtgtcacacc ccaggaatcg cgtcactgaa cacagcagcc ggtaggacga
3961 ccatgactga gttggacacc atcgcaaatc cgtccgatcc cgcggtgcag cggatcatcg
4021 atgtcaccaa gccgtcgcga tccaacataa agacaacgtt gatcgaggac gtcgagcccc
4081 tcatgcacag catcgcggcc ggggtggagt tcatcgaggt ctacggcagc gacagcagtc
4141 cttttccatc tgagttgctg gatctgtgcg ggcggcagaa cataccggtc cgcctcatcg
4201 actcctcgat cgtcaaccag ttgttcaagg gggagcggaa ggccaagaca ttcggcatcg
4261 cccgcgtccc tcgcccggcc aggttcggcg atatcgcgag ccggcgtggg gacgtcgtcg
4321 ttctcgacgg ggtgaagatc gtcgggaaca tcggcgcgat agtacgcacg tcgctcgcgc
4381 tcggagcgtc ggggatcatc ctggtcgaca gtgacatcac cagcatcgcg gaccggcgtc
4441 tccaaagggc cagccgaggt tacgtcttct cccttcccgt cgttctctcc ggtcgcgagg
4501 aggccatcgc cttcattcgg gacagcggta tgcagctgat gacgctcaag gcggatggcg
4561 acatttccgt gaaggaactc ggggacaatc cggatcggct ggccttgctg ttcggcagcg
4621 aaaagggtgg gccttccgac ctgttcgagg aggcgtcttc cgcctcggtt tccatcccca
4681 tgatgagcca gaccgagtct ctcaacgttt ccgtttccct cggaatcgcg ctgcacgaga
4741 ggatcgacag gaatctcgcg gccaaccgat aagcgcctct gttcctcgga cgctcggttg
4801 ctcgacctcg attcgtcagt gatgatcaac aggacccaga aatggcacga gcccgggatt
4861 ggcctcccgg gctcgccgcc cccgaaccgg cgcagcatgc cgagtgagga cgtttgctct
4921 gtgacgagcg tacgagagaa cagcgcgtcg acaccaccgc gccccgccgg accgaagtgc
4981 cactgcggca ccccgatcgt ccacatcccc gggaagcgcc ccaaaatcta ctgctcggac
5041 gcctgccgaa agcgcgcaaa gcgtctcatt gcggaccagt cacgcagcgt agcaagcacg
5101 actacagcgg gtaatccgga cgcgggcttt agtagtaagg gaaaatctcc gtccactgca
5161 ggtcagagcg ctgtaactgg aacgggttct accccaaacg agggtgccgc gacagccggg
5221 cgggaggacc cgcgcgacga gcggttcgcc cggcgggacc ggcaccagac ggtgtcgctg
5281 aacgaggcgt tcaagggctg cgggacccgg ctcactgccg ggacggccga gctgatgtgg
5341 aagccgggcg aggcgacgtg gggcaacacg tgccgctgca acaacgtcca cacctgcccg
5401 tggtgcatga gccgcatcct ggccgtacgg ggctcgaacg tgcagctcgc cgccgacggt
5461 ctcgcggacg ccgggtacgg gctgcacctt ggcacgaaca cgttgcgcca cttcgagcgc
5521 atggcgttcg gcaccgtccg caagggaatg cggcacggcc tggtcgcggt cttgcacgac
5581 ggctggaagg gcgcgtacgg ctcgtccggc cgccgctggc gcacgatgcg cgacgacttc
5641 gggatcatcg gctacgagcg ggccttcgag gacaccttcg gctggggctc cggctggcac
5701 ctgcactggc acacgctctg ggtcacccgc gaggtgctcg gcccggacgc ccaggccgcg
5761 ttccgcgacg cactcgccgg agcctgggcc gccggggtcg aatccgcggg cggctacacc
5821 gtcagcgaga cgtgcgaccg gcccggttgc tcctgtgagg gcaagggcca cggcaccgac
5881 gtgcgcccac tcaacggcgc ggacgccgct gacggcgatg cgggcaagca ggcccgctac
5941 ctctacaagg acggcgacaa gaccaagggc ggcgtcgcca agatcggcct ggagctggcc
6001 ggacagaact tcaaggccgg tcgcggcgac gaccgcatgg gaccgctcga cctgggcgac
6061 gcggcggccg ccgagctgca gcggcttcgt cggcccggcc cgttcgtcga gaagtaccgc
6121 gagcgcgagt tcggcgtctt ccaggtccgc aagcactacc ggtcgcagaa cctgaaccgg
6181 ctgatcaagg aactcggcat ccagcaggac gtccgcaccg aggaggagat caccgacgac
6241 accgagggcc tggtcgccat cgcggtcatc ccggcctaca tctggtaccg atacatcgcc
6301 cgcgtcgccg gacgtcgcct cgacctcatc aaggtcgccg agacgtacgg cctaccgggc
6361 gtccggcggc tcgtcgagtc ctggggcctg gtgtggggca aggacgtcct ggacccgccc
6421 gccccggaag cgccggccgc gccgggcgac cttgacgctg accagatgag gttcgaggtc
6481 atgtccgagg aggaagctgc tttccgcgag gcgcgccgga aggccaacga ggcccgcacg
6541 gaggagctgg ccgcttcgct cgaccgagtg cgtcagccga agaaggaggc gatccgcccg
6601 acgatcagcc ttcggaagcg cctgaagccg aagccggtca ccgtcgacgt caagactccc
6661 ccgcccggcg ccgcgtcgcc cgtgtgccgc cgctgcaagg gcaagctcgc cccggtactc
6721 cagccctggg gccggctccc cggagactgc ctccgcgtcg acacagcggt cgcgtagggc
6781 cgctgaggga ccgccatgag accgccaccc cggacatccg ggtggcggtc tcatggcggt
6841 cccggcgcga aagccgaggg tctgcctgcc gtgaggtggc cggcggacct gggttactgc
6901 tggaatcccg cgcccagtcc gagcgtccgc cggcgctttt cacctggctc cgggtgctcg
6961 tcgatgagca cca
//
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