| 载体名称: | pDG148 |
|---|---|
| 质粒类型: | 大肠杆菌质粒；枯草杆菌质粒 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 多克隆位点 |
| 启动子: | Pspac |
| 载体大小: | 8.7kb |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 培养条件: | 37°度 |
| 备注: | 枯草杆菌表达质粒 |
| 产品目录号: | -- |
| 稳定性: | 稳定 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pDG148载体质粒基本信息
pDG148质粒图谱载体图谱和pDG148载体序列质粒序列多克隆位点信息
pDG148质粒载体简介
pDG148质粒序列载体序列
LOCUS pDG148 8750 bp DNA circular SYN 18-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8750)
AUTHORS Ming
TITLE Direct Submission
JOURNAL Exported Jul 18, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8750
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 465..481
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 489..497
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 766..1848
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKTAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
misc_feature 2019..2159
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(2345..2933)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3104..3964)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3965..4069)
/gene="bla"
/label=AmpR promoter
CDS 4588..5592
/codon_start=1
/gene="repB"
/product="RepB replication protein"
/label=repB
/note="from Enterococcus faecalis plasmid pAM-alpha-1"
/db_xref="GI:22652809"
/protein_id="AAN03827.1"
/translation="MGFSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
ORIGIN
1 cgggaaaaac gggaagtagg ctcccatgat aaaaaagtaa aagaaaaaga ataaaccgaa
61 catccaaaag tttgtgtttt ttaaatagta cataatggat ttccttacgc gaaatacggg
121 cagacatggc ctgcccggtt attattattt ttgacaccag accaactggt aatggtagcg
181 accggcgctc agctggaatt ctacacagcc cagtccagac tattcggcac tgaaattatg
241 ggtgaagtgg tcaagacctc actaggcacc ttaaaaatag cgcaccctga agaagattta
301 tttgaggtag cccttgccta cctagcttcc aagaaagata tcctaacagc acaagagcgg
361 aaagatgttt tgttctacat ccagaacaac ctctgctaaa attcctgaaa aattttgcaa
421 aaagttgttg actttatcta caaggtgtgg cataatgtgt ggaattgtga gcggataaca
481 attaagctta aggaggtgat ctagagtcga cctgcaggca tgcaagctaa ttcggtggaa
541 acgaggtcat catttccttc cgaaaaaacg gttgcattta aatcttacat atgtaatact
601 ttcaaagact acatttgtaa gatttgatgt ttgagtcggc tgaaagatcg tacgtaccaa
661 ttattgtttc gtgattgttc aagccataac actgtaggga tagtggaaag agtgcttcat
721 ctggttacga tcaatcaaat attcaaacgg agggagacga ttttgatgaa accagtaacg
781 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac
841 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg
901 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc
961 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct
1021 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa
1081 gcctgtaaaa cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac
1141 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg
1201 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac
1261 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta
1321 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc
1381 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt
1441 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc
1501 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt
1561 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg
1621 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg
1681 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa
1741 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
1801 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
1861 taatgtgagt taggatcctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
1921 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
1981 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
2041 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
2101 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
2161 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
2221 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
2281 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
2341 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
2401 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
2461 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
2521 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
2581 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
2641 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
2701 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
2761 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
2821 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
2881 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
2941 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
3001 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
3061 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
3121 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
3181 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
3241 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
3301 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
3361 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
3421 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
3481 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
3541 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
3601 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
3661 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
3721 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
3781 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
3841 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
3901 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
3961 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
4021 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
4081 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
4141 ggcgtatcac gaggcccttt cgtcttcaag aattccttaa ggaacgtaca gacggcttaa
4201 aagcctttaa aaacgttttt aaggggtttg tagacaaggt aaaggataaa acagcacaat
4261 tccaagaaaa acacgattta gaacctaaaa agaacgaatt tgaactaact cataaccgag
4321 aggtaaaaaa agaacgaagt cgagatcagg gaatgagttt ataaaataaa aaaagcacct
4381 gaaaaggtgt ctttttttga tggttttgaa cttgttcttt cttatcttga tacatataga
4441 aataacgtca tttttatttt agttgctgaa aggtgcgttg aagtgttggt atgtatgtgt
4501 tttaaagtat tgaaaaccct taaaattggt tgcacagaaa aaccccatct gttaaagtta
4561 taagtgacta aacaaataac taaatagatg gggttttctt ttaatattat gtgtcctaat
4621 agtagcattt attcagatga aaaatcaagg gttttagtgg acaagacaaa aagtggaaaa
4681 gtgagaccat ggagagaaaa gaaaatcgct aatgttgatt actttgaact tctgcatatt
4741 cttgaattta aaaaggctga aagagtaaaa gattgtgctg aaatattaga gtataaacaa
4801 aatcgtgaaa caggcgaaag aaagttgtat cgagtgtggt tttgtaaatc caggctttgt
4861 ccaatgtgca actggaggag agcaatgaaa catggcattc agtcacaaaa ggttgttgct
4921 gaagttatta aacaaaagcc aacagttcgt tggttgtttc tcacattaac agttaaaaat
4981 gtttatgatg gcgaagaatt aaataagagt ttgtcagata tggctcaagg atttcgccga
5041 atgatgcaat ataaaaaaat taataaaaat cttgttggtt ttatgcgtgc aacggaagtg
5101 acaataaata ataaagataa ttcttataat cagcacatgc atgtattggt atgtgtggaa
5161 ccaacttatt ttaagaatac agaaaactac gtgaatcaaa aacaatggat tcaattttgg
5221 aaaaaggcaa tgaaattaga ctatgatcca aatgtaaaag ttcaaatgat tcgaccgaaa
5281 aataaatata aatcggatat acaatcggca attgacgaaa ctgcaaaata tcctgtaaag
5341 gatacggatt ttatgaccga tgatgaagaa aagaatttga aacgtttgtc tgatttggag
5401 gaaggtttac accgtaaaag gttaatctcc tatggtggtt tgttaaaaga aatacataaa
5461 aaattaaacc ttgatgacac agaagaaggc gatttgattc atacagatga tgacgaaaaa
5521 gccgatgaag atggattttc tattattgca atgtggaatt gggaacggaa aaattatttt
5581 attaaagagt agttcaacaa acgggccagt ttgttgaaga ttagatgcta taattgttat
5641 taaaaggatt gaaggatgct taggaagacg agttattaat agctgaataa gaacggtgct
5701 ctccaaatat tcttatttag aaaagcaaat ctaaaattat ctgaaaaggg aatgagaata
5761 gtgaatggac caataataat gactagagaa gaaagaatga agattgttca tgaaattaag
5821 gaacgaatat tggataaata tggggatgat gttaaggcta ttggtgttta tggctctctt
5881 ggtcgtcaga ctgatgggcc ctattcggat attgagatga tgtgtgtcat gtcaacagag
5941 gaagcagagt tcagccatga atggacaacc ggtgagtgga aggtggaagt gaattttgat
6001 agcgaagaga ttctactaga ttatgcatct caggtggaat cagattggcc gcttacacat
6061 ggtcaatttt tctctatttt gccgatttat gattcaggtg gatacttaga gaaagtgtat
6121 caaactgcta aatcggtaga agcccaaacg ttccacgatg cgatttgtgc ccttatcgta
6181 gaagagctgt ttgaatatgc aggcaaatgg cgtaatattc gtgtgcaagg accgacaaca
6241 tttctaccat ccttgactgt acaggtagca atggcaggtg ccatgttgat tggtctgcat
6301 catcgcatct gttatacgac gagcgcttcg gtcttaactg aagcagttaa gcaatcagat
6361 cttccttcag gttatgacca tctgtgccag ttcgtaatgt ctggtcaact ttccgactct
6421 gagaaacttc tggaatcgct agagaatttc tggaatggga ttcaggagtg gacagaacga
6481 cacggatata tagtggatgt gtcaaaacgc ataccatttt gaacgatgac ctctaataat
6541 tgttaatcat gttggttacg tatttattaa cttctcctag tattagtaat tatcatggct
6601 gtcatggcgc attaacggaa taaagggtgt gcttaaatcg ggccattttg cgtaataaga
6661 aaaaggatta attatgagcg aattgaatta ataataaggt aatagattta cattagaaaa
6721 tgaaagggga ttttatgcgt gagaatgtta cagtctatcc cggcattgcc agtcggggat
6781 attaaaaaga gtataggttt ttattgcgat aaactaggtt tcactttggt tcaccatgaa
6841 gatggattcg cagttctaat gtgtaatgag gttcggattc atctatggga ggcaagtgat
6901 gaaggctggc gctctcgtag taatgattca ccggtttgta caggtgcgga gtcgtttatt
6961 gctggtactg ctagttgccg cattgaagta gagggaattg atgaattata tcaacatatt
7021 aagcctttgg gcattttgca ccccaataca tcattaaaag atcagtggtg ggatgaacga
7081 gactttgcag taattgatcc cgacaacaat ttgattagct tttttcaaca aataaaaagc
7141 taaaatctat tattaatctg ttcagcaatc gggcgcgatt gctgaataaa agatacgaga
7201 gacctctctt gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa
7261 gacaataaaa attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa
7321 aataaaaatt ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac
7381 tacctgtccc ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg
7441 gcgaacgatg agccaataat acgtacagac ataatagtgc cagccagcag cagggcgttt
7501 ttcccaccga tgcgattaat gatcagtggc gcaaagaaca taatcgaggc gttaagtaat
7561 tcgcccattg tcgttacgta gccaaatacc cgcgtaccct gttcaccggt agcaaagaac
7621 gaagtaaaga aattagcaaa ctgttggtca aaaacatcgt aggtgcagga aacgccaata
7681 acatacagtg acaaaaacca cagttttggc tgtctgaaca gttccagtgc cagcttaagg
7741 ctaaatgccg aatggttggc acctaccgca ttggcaaccg tggcagaaga gggcgcatcc
7801 gttttggcga aaaagagtaa aacggcgagg atgagtgcac agccagagcc cagccagaaa
7861 acaaactgat tattgatggt gaacatgatg ccgacaatcg aggcacacag cgcccagcca
7921 acacagccaa acatccgcgc gcgaccaaat tcgaaattac tgcgacggct gactttctca
7981 ataaatgcct ctactgctgg cgcaccggcg ttaaaacaaa agcctagata aataccacca
8041 acaatcgatc ctactaaaat gttgtattgt aacagtggcc cgaagataaa aataaagaac
8101 ggcgcaaaca tcactaacat gccggtaata atccacagca ggtatttgcg cagcccgagt
8161 ttgtcagaaa gcagaccaaa cagcggttgg aataatagcg agaacagaga aatagcggca
8221 aaaataatac ccgtatcact tttgctgata tggttgatgt catgtagcca aatcgggaaa
8281 aacgggaagt aggctcccat gataaaaaag taaaagaaaa agaataaacc gaacatccaa
8341 aagtttgtgt tttttaaata gtacataatg gatttcctta cgcgaaatac gggcagacat
8401 ggcctgcccg gttattatta tttttgacac cagaccaact ggtaatggta gcgaccggcg
8461 ctcagctgga attctacaca gcccagtcca gactattcgg cactgaaatt atgggtgaag
8521 tggtcaagac ctcactaggc accttaaaaa tagcgcaccc tgaagaagat ttatttgagg
8581 tagcccttgc ctacctagct tccaagaaag atatcctaac agcacaagag cggaaagatg
8641 ttttgttcta catccagaac aacctctgct aaaattcctg aaaaattttg caaaaagttg
8701 ttgactttat ctacaaggtg tggcataatg tgtggaattg tgagcggata
//
