| 质粒名称: | pGKO1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 63611 |
| 存储实验室: | Seogchan Kang |
|
载体骨架基本信息 |
|
| 载体名称: | pCAMBIA1300 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | 携带ChGPD HSVtk的1.8 kb EcoRI HindIII片段通过Klenow片段和dNTPs处理而变钝，并在pCAMBIA1300的XhoI和BstXI位点之间克隆，这两个位点用T4 DNA聚合酶和dNTPs钝化-注意，钝化的HindIII和BstXI位点的连接产生了不需要的HindIII位点。 |
| 载体类型: | 二元真菌载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | Lac Promoter |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | M13-F20 |
| 3' 测序引物 | M13-R |
|
文献引用方法 |
|
| 材料和方法部分: | pGKO1 was a gift from Seogchan Kang (Addgene plasmid # 63611 ; http://n2t.net/addgene:63611 ; RRID:Addgene_63611) |
| 参考文献部分: | A dual selection based, targeted gene replacement tool for Magnaporthe grisea and Fusarium oxysporum. Khang CH, Park SY, Lee YH, Kang S. Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25. 10.1016/j.fgb.2005.03.004 PubMed 15893252 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGKO1 8931 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION a binary Agrobacterium vector carrying the HSVtk gene as a negative
selection marker against ectopic transformants..
ACCESSION .
VERSION .
KEYWORDS pGKO1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8931)
AUTHORS Khang CH, Park SY, Lee YH, Kang S
TITLE A dual selection based, targeted gene replacement tool for
Magnaporthe grisea and Fusarium oxysporum.
JOURNAL Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25.
PUBMED 15893252
REFERENCE 2 (bases 1 to 8931)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8931
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 1..25
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 103..277
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS 717..1847
/codon_start=1
/product="herpes simplex virus thymidine kinase"
/label=HSV TK
/note="confers sensitivity to nucleoside analogs such as
acyclovir or ganciclovir"
/translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
protein_bind 2252..2273
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2288..2318
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2326..2342
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2331..2353
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2350..2366
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2350..2366
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
misc_feature 2375..2431
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(2435..2452)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(2435..2451)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2444..2466)
/label=M13/pUC Forward
/note="In lacZ gene"
misc_feature 2654..2678
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 3978..4607
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 5041..6108
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
rep_origin 6174..6368
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(6604..6626)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 6712..6852
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 6767..6786
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(6867..6884)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7038..7626)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7118..7137)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7713..8507)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
241 cttatctggg aactactcac acattattat ggagaaactc gaaattcgaa ttgggtactc
301 aaattggttc ccgcttgacg acattccgaa acccccaatt tcgcggcttc gaatcgtggc
361 tacccttggc cgaggttgcg atttctctgc cgtatctgac aatgatccaa ccggccggat
421 gtgtgggagg gcgcaaaggg tgtaattggg tctaggcgtg agctgcatgt ccgatggcag
481 aaaaagaact actaaaactc tgtgacgtcc tcagggctgg tagctattta gcttgcccct
541 ccctcacccc acctagctca aacccaacaa aacacagcac cgaccacaaa aatctgcctc
601 ttaccacctg ctcatcacct tttctcacat ataaagcagt gagctcagcc cgatttccat
661 tcctcaattc aagtctatta actctctcaa agaggaaccc aatcttcaaa ggatccatgg
721 cttcgtaccc ctgccatcaa cacgcgtctg cgttcgacca ggctgcgcgt tctcgcggcc
781 atagcaaccg acgtacggcg ttgcgccctc gccggcagca agaagccacg gaagtccgcc
841 tggagcagaa aatgcccacg ctactgcggg tttatataga cggtcctcac gggatgggga
901 aaaccaccac cacgcaactg ctggtggccc tgggttcgcg cgacgatatc gtctacgtac
961 ccgagccgat gacttactgg caggtgctgg gggcttccga gacaatcgcg aacatctaca
1021 ccacacaaca ccgcctcgac cagggtgaga tatcggccgg ggacgcggcg gtggtaatga
1081 caagcgccca gataacaatg ggcatgcctt atgccgtgac cgacgccgtt ctggctcctc
1141 atatcggggg ggaggctggg agctcacatg ccccgccccc ggccctcacc ctcatcttcg
1201 accgccatcc catcgccgcc ctcctgtgct acccggccgc gcgatacctt atgggcagca
1261 tgacccccca ggccgtgctg gcgttcgtgg ccctcatccc gccgaccttg cccggcacaa
1321 acatcgtgtt gggggccctt ccggaggaca gacacatcga ccgcctggcc aaacgccagc
1381 gccccggcga gcggcttgac ctggctatgc tggccgcgat tcgccgcgtt tacgggctgc
1441 ttgccaatac ggtgcggtat ctgcagggcg gcgggtcgtg gcgggaggat tggggacagc
1501 tttcggggac ggccgtgccg ccccagggtg ccgagcccca gagcaacgcg ggcccacgac
1561 cccatatcgg ggacacgtta tttaccctgt ttcgggcccc cgagttgctg gcccccaacg
1621 gcgacctgta caacgtgttt gcctgggcct tggacgtctt ggccaaacgc ctccgtccca
1681 tgcacgtctt tatcctggat tacgaccaat cgcccgccgg ctgccgggac gccctgctgc
1741 aacttacctc cgggatggtc cagacccacg tcaccacccc cggctccata ccgacgatct
1801 gcgacctggc gcgcacgttt gcccgggaga tgggggaggc taactgagtc gacctgcagg
1861 catgcatcat tccactcaac attcaggctc ctctgcgcac gtaaagtgcc aaaggcaata
1921 ccctgctcgg tggaatgccg ccgggcttgt cgattttacg cacatatgcg cattcttgac
1981 ttgaagcgga ggagttcttc gttgcgggtt acagtgtttt aataaaagaa tggtcaaatc
2041 aaactgctag atatacctgt cagacactct agttgttgac ccctatactc ttaatacatc
2101 agacagtaca tgcatgttgc atgatgataa gctttggcaa gctgctctag ccaatacgca
2161 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
2221 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
2281 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
2341 aatttcacac aggaaacagc tatgacatga ttacgaattc gagctcggta cccggggatc
2401 ctctagagtc gacctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg
2461 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
2521 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
2581 atggcgaatg ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta
2641 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
2701 agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
2761 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
2821 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
2881 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
2941 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
3001 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
3061 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
3121 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
3181 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
3241 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
3301 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
3361 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
3421 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
3481 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
3541 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
3601 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
3661 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
3721 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
3781 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
3841 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
3901 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
3961 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
4021 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
4081 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
4141 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
4201 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
4261 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
4321 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
4381 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
4441 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
4501 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
4561 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
4621 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
4681 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
4741 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
4801 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
4861 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
4921 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
4981 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgac
5041 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
5101 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
5161 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
5221 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
5281 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
5341 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
5401 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
5461 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
5521 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
5581 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
5641 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
5701 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
5761 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
5821 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
5881 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
5941 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
6001 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
6061 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
6121 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
6181 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
6241 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
6301 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
6361 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
6421 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
6481 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
6541 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
6601 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
6661 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
6721 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
6781 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
6841 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
6901 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
6961 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
7021 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
7081 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
7141 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
7201 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
7261 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
7321 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
7381 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
7441 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
7501 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
7561 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
7621 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
7681 cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa
7741 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
7801 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
7861 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
7921 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
7981 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
8041 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
8101 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
8161 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
8221 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
8281 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
8341 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
8401 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
8461 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
8521 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
8581 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
8641 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
8701 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
8761 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
8821 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
8881 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg g
//
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