pLXSN

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLXSN
质粒类型: 哺乳动物细胞表达载体;逆病毒载体
高拷贝/低拷贝: 低拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: --
载体大小: 5874 bp
5' 测序引物及序列: 5’-CCCTTGAACCTCCTCGTTCGACC-3’
3' 测序引物及序列: 5’-GAGCCTGGGGACTTTCCACACCC-3’
载体标签:
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: DH5α, HB101
宿主细胞(系): 常规细胞系
备注: --
产品目录号: 631509
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pLXSN载体图谱



pLXSN 多克隆位点

pLXSN 载体特征

载体简介

pLXSN 载体描述
pLXSN contains elements derived from Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus (MoMuSV), and is designed for retroviral gene delivery and expression (1–3). Upon transfection into a packaging cell line, pLXSN can transiently express, or integrate and stably express, a transcript containing Ψ+ (the extended viral packaging signal) the gene of interest, and a selectable marker. The 5’ viral LTR in this vector contains promoter/ enhancer sequences that control expression of the gene of interest in the multiple cloning site. The SV40 early promoter (PSV40e) controls expression of the neomycin resistance gene (Neor), which allows antibiotic selection in eukaryotic cells. pLXSN also includes the Col E1 origin of replication and E. coli Ampr gene for propagation and antibiotic selection in bacteria.


As a part of the Retro-X System (Cat. No. 631508), pLXSN can be transfected into a packaging cell line such as the RetroPack PT67 Cell Line (Cat. No. 631510). Once in the cell, RNA from the vector is packaged into infectious, replication-incompetent retroviral particles. pLXSN does not contain the structural genes (gag, pol, and env) necessary for particle formation and replication, however, these genes are stably integrated into PT67 (4–7).Subsequent introduction of pLXSN, containing Ψ+ (psi), transcription and processing elements, and the gene of interest produces high-titer, replication-incompetent infectious virus. That is, these retroviral particles can infect target cells and transmit the gene of interest (which is cloned between the viral LTR sequences), but cannot replicate within these cells since the cells lack the viral structural genes. The separate introduction and integration of the structural genes into the packaging cell line minimizes the chances of producing replication-competent virus due to recombination events during cell proliferation.

载体序列

LOCUS       pLXSN	5874 bp 	DNA	SYN
DEFINITION  pLXSN
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="pLXSN"
                     /mol_type="other DNA"
     misc_feature    175..763
                     /label="5MoMuLV_LTR"
     misc_feature    833..1642
                     /label="psi_plus_pack"
     misc_feature    1229..1636
                     /label="gag"
     misc_feature    1572..1594
                     /label="MSCV_primer"
     misc_feature    1572..1594
                     /label="pLXSN_5_primer"
     misc_feature    1610..1626
                     /label="pBABE_5_primer"
     misc_feature    complement(1672..1692)
                     /label="pBABE_3_primer"
     misc_feature    complement(1678..1893)
                     /label="SV40_enhancer"
     promoter        1690..1958
                     /label="SV40_promoter"
     rep_origin      1857..1934
                     /label="SV40_origin"
     misc_feature    1919..1938
                     /label="SV40pro_F_primer"
     CDS             2066..2860
                     /label="ORF frame 2"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            2069..2857
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    2920..3513
                     /label="3MoMuLV_LTR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    2962..3513
                     /label="5_LTR2"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    complement(3616..3638)
                     /label="pGEX_3_primer"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            complement(4809..5669)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(4809..5669)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5711..5739)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
   61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
  121 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa
  181 agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa
  241 atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata
  301 ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag
  361 acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg
  421 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca
  481 gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca
  541 atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc
  601 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat
  661 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg
  721 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga
  781 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
  841 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta
  901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
  961 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
 1021 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
 1081 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
 1141 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
 1201 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
 1261 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
 1321 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
 1381 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
 1441 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
 1501 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
 1561 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
 1621 cctcactcct tctctaggcg ccggaattcg ttaactcgag gatccggctg tggaatgtgt
 1681 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
 1741 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
 1801 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
 1861 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
 1921 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
 1981 tttttggagg cctaggcttt tgcaaaaagc ttgggctgca ggtcgaggcg gatctgatca
 2041 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
 2101 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
 2161 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
 2221 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
 2281 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
 2341 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
 2401 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
 2461 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
 2521 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
 2581 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
 2641 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
 2701 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
 2761 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
 2821 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgat
 2881 aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag
 2941 gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag
 3001 aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag
 3061 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga
 3121 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
 3181 agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc
 3241 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc
 3301 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct
 3361 cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa
 3421 ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag
 3481 tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga tcgggagacc
 3541 cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggctgcctcg cgcgtttcgg
 3601 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
 3661 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
 3721 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
 3781 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
 3841 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
 3901 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
 3961 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
 4021 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
 4081 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
 4141 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
 4201 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
 4261 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
 4321 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
 4381 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
 4441 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
 4501 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
 4561 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
 4621 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
 4681 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
 4741 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
 4801 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
 4861 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
 4921 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
 4981 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
 5041 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
 5101 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg
 5161 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
 5221 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
 5281 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
 5341 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
 5401 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
 5461 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
 5521 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
 5581 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
 5641 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
 5701 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
 5761 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
 5821 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcaa

//

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