| 出品公司: | Clontech |
|---|---|
| 载体名称: | pCMV-(DYKDDDDK)-N |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 3779 dp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | FLAG N-term |
| 载体抗性: | 氨苄霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 哺乳动物细胞中表达N-末端标记蛋白的载体。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | 非病毒 |
买家导航
pCMV-(DYKDDDDK)-N载体质粒基本信息
pCMV-(DYKDDDDK)-N质粒图谱载体图谱和pCMV-(DYKDDDDK)-N载体序列质粒序列多克隆位点信息
-N载体图谱.png)
pCMV-(DYKDDDDK)-N质粒载体简介
pCMV-(DYKDDDDK)-N质粒序列载体序列
LOCUS pCMV-(DYKDDDDK)-N 3779 bp ds-DNA circular SYN 19-OCT-2017
DEFINITION Vector for expressing an N-terminally FLAG-tagged protein in
mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCMV-(DYKDDDDK)-N
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3779)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Saturday, November 30, 2019 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3779
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 27..330
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 331..534
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 672..768
/note="SV40 intron"
/note="modified SV40 intron with splice donor and acceptor
sites"
CDS 829..831
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 832..855
/codon_start=1
/product="FLAG epitope tag, followed by an enterokinase
cleavage site"
/note="FLAG"
/translation="DYKDDDDK"
misc_feature 860..914
/label=MCS
/note="MCS"
/note="multiple cloning site"
polyA_signal 918..1052
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(1163..1179)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1187..1203
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1211..1241)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
rep_origin complement(1565..2153)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2324..3184)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3185..3289)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat ggactacaag
841 gacgacgatg acaagcttat ggccatggag gcccgaattc ggtcgaccga gatctctcga
901 ggtaccgcgg ccgcggggat ccagacatga taagatacat tgatgagttt ggacaaacca
961 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
1021 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
1081 ttcaggttca gggggaggtg tgggaggttt tttcggatcc tctagagtcg atctgcaggc
1141 atgctagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
1201 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
1261 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
1321 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
1381 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
1441 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
1501 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
1561 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
1621 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
1681 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
1741 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
1801 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
1861 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
1921 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
1981 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
2041 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
2101 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
2161 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
2221 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
2281 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
2341 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
2401 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
2461 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
2521 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
2581 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
2641 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
2701 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
2761 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
2821 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
2881 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
2941 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
3001 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
3061 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
3121 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
3181 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
3241 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
3301 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
3361 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
3421 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
3481 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
3541 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
3601 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
3661 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
3721 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagt
//
pCMV-(DYKDDDDK)-N载体图谱质粒图谱pdf版和pCMV-(DYKDDDDK)-N载体序列质粒序列等相关资料下载
pCMV-(DYKDDDDK)-N质粒载体应用举例
- 上一篇：pCMV-(DYKDDDDK)-C
- 下一篇：pCMV-HA-C
