| 质粒名称: | pVSV-G |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 138479 |
| 存储实验室: | Akitsu Hotta |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV vector |
| 空载体大小: | 4972 bp |
| 完整质粒大小: | 6508bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | VSV-G envelop protein/vesicular stomatitis virus glycoprotein |
| 物种来源: | Vesicular stomatitis virus |
|
文献引用方法 |
|
| 材料和方法部分: | pVSV-G was a gift from Akitsu Hotta (Addgene plasmid # 138479 ; http://n2t.net/addgene:138479 ; RRID:Addgene_138479) |
| 参考文献部分: | Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping. Gee P, Lung MSY, Okuzaki Y, Sasakawa N, Iguchi T, Makita Y, Hozumi H, Miura Y, Yang LF, Iwasaki M, Wang XH, Waller MA, Shirai N, Abe YO, Fujita Y, Watanabe K, Kagita A, Iwabuchi KA, Yasuda M, Xu H, Noda T, Komano J, Sakurai H, Inukai N, Hotta A. Nat Commun. 2020 Mar 13;11(1):1334. doi: 10.1038/s41467-020-14957-y. 10.1038/s41467-020-14957-y PubMed 32170079 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pVSV-G 6507 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses VSV-G envelop protein for pseudotyping NanoMEDIC
particle..
ACCESSION .
VERSION .
KEYWORDS pVSV-G
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6507)
AUTHORS Gee P, Lung MSY, Okuzaki Y, Sasakawa N, Iguchi T, Makita Y, Hozumi
H, Miura Y, Yang LF, Iwasaki M, Wang XH, Waller MA, Shirai N, Abe
YO, Fujita Y, Watanabe K, Kagita A, Iwabuchi KA, Yasuda M, Xu H,
Noda T, Komano J, Sakurai H, Inukai N, Hotta A
TITLE Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and
sgRNA to induce therapeutic exon skipping.
JOURNAL Nat Commun. 2020 Mar 13;11(1):1334. doi: 10.1038/s41467-020-14957-y.
PUBMED 32170079
REFERENCE 2 (bases 1 to 6507)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6507
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..204
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 155..175
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 201..225
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 316..888
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
CDS 974..2509
/codon_start=1
/product="vesicular stomatitis virus G glycoprotein"
/label=VSV-G
/note="Indiana strain"
/translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
primer_bind complement(2736..2755)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 2801..2856
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(2855..2874)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(3214..3230)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3214..3230)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3227..3249)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3238..3254
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3262..3292)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3307..3328
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3445..3462)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3616..4204)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3696..3715)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4375..5235)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4998..5017
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5236..5340)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5366..5821)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5503..5524)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 5715..5734
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5948..5970
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 5962..5979
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 5963..5979
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 5989..6008
/label=T7
/note="T7 promoter, forward primer"
promoter 5989..6007
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
enhancer 6128..6507
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg gtcgaccgat
301 cctgagaact tcagggtgag tttggggacc cttgattgtt ctttcttttt cgctattgta
361 aaattcatgt tatatggagg gggcaaagtt ttcagggtgt tgtttagaat gggaagatgt
421 cccttgtatc accatggacc ctcatgataa ttttgtttct ttcactttct actctgttga
481 caaccattgt ctcctcttat tttcttttca ttttctgtaa ctttttcgtt aaactttagc
541 ttgcatttgt aacgaatttt taaattcact tttgtttatt tgtcagattg taagtacttt
601 ctctaatcac ttttttttca aggcaatcag ggtatattat attgtacttc agcacagttt
661 tagagaacaa ttgttataat taaatgataa ggtagaatat ttctgcatat aaattctggc
721 tggcgtggaa atattcttat tggtagaaac aactacaccc tggtcatcat cctgcctttc
781 tctttatggt tacaatgata tacactgttt gagatgagga taaaatactc tgagtccaaa
841 ccgggcccct ctgctaacca tgttcatgcc ttcttctctt tcctacagct cctgggcaac
901 gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat tcctcgacgg atccctcgag
961 gaattctgac actatgaagt gccttttgta cttagccttt ttattcattg gggtgaattg
1021 caagttcacc atagtttttc cacacaacca aaaaggaaac tggaaaaatg ttccttctaa
1081 ttaccattat tgcccgtcaa gctcagattt aaattggcat aatgacttaa taggcacagc
1141 cttacaagtc aaaatgccca agagtcacaa ggctattcaa gcagacggtt ggatgtgtca
1201 tgcttccaaa tgggtcacta cttgtgattt ccgctggtat ggaccgaagt atataacaca
1261 ttccatccga tccttcactc catctgtaga acaatgcaag gaaagcattg aacaaacgaa
1321 acaaggaact tggctgaatc caggcttccc tcctcaaagt tgtggatatg caactgtgac
1381 ggatgccgaa gcagtgattg tccaggtgac tcctcaccat gtgctggttg atgaatacac
1441 aggagaatgg gttgattcac agttcatcaa cggaaaatgc agcaattaca tatgccccac
1501 tgtccataac tctacaacct ggcattctga ctataaggtc aaagggctat gtgattctaa
1561 cctcatttcc atggacatca ccttcttctc agaggacgga gagctatcat ccctgggaaa
1621 ggagggcaca gggttcagaa gtaactactt tgcttatgaa actggaggca aggcctgcaa
1681 aatgcaatac tgcaagcatt ggggagtcag actcccatca ggtgtctggt tcgagatggc
1741 tgataaggat ctctttgctg cagccagatt ccctgaatgc ccagaagggt caagtatctc
1801 tgctccatct cagacctcag tggatgtaag tctaattcag gacgttgaga ggatcttgga
1861 ttattccctc tgccaagaaa cctggagcaa aatcagagcg ggtcttccaa tctctccagt
1921 ggatctcagc tatcttgctc ctaaaaaccc aggaaccggt cctgctttca ccataatcaa
1981 tggtacccta aaatactttg agaccagata catcagagtc gatattgctg ctccaatcct
2041 ctcaagaatg gtcggaatga tcagtggaac taccacagaa agggaactgt gggatgactg
2101 ggcaccatat gaagacgtgg aaattggacc caatggagtt ctgaggacca gttcaggata
2161 taagtttcct ttatacatga ttggacatgg tatgttggac tccgatcttc atcttagctc
2221 aaaggctcag gtgttcgaac atcctcacat tcaagacgct gcttcgcaac ttcctgatga
2281 tgagagttta ttttttggtg atactgggct atccaaaaat ccaatcgagc ttgtagaagg
2341 ttggttcagt agttggaaaa gctctattgc ctcttttttc tttatcatag ggttaatcat
2401 tggactattc ttggttctcc gagttggtat ccatctttgc attaaattaa agcacaccaa
2461 gaaaagacag atttatacag acatagagat gaaccgactt ggaaagtaac tcaaatcctg
2521 cacaacagat tcttcatgtt tggaccaaat caacttgtga taccatgctc aaagaggcct
2581 caattatatt tgagttttta atttttatga aaaaaaaaaa aaaaaacgga attcctcgag
2641 ggatccgtcg aggaattcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
2701 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
2761 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
2821 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
2881 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
2941 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
3001 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
3061 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
3121 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
3181 tccctcgacg gatcggccgc aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
3241 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
3301 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
3361 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
3421 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
3481 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
3541 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
3601 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
3661 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
3721 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
3781 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
3841 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
3901 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
3961 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
4021 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
4081 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
4141 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
4201 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
4261 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
4321 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
4381 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
4441 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
4501 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
4561 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
4621 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
4681 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
4741 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
4801 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
4861 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
4921 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
4981 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
5041 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
5101 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
5161 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
5221 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
5281 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
5341 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
5401 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
5461 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
5521 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
5581 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
5641 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
5701 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
5761 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
5821 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
5881 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
5941 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca
6001 ctatagggcg aattggagct ccaccgcggt ggcggccgct ctagagagct tggcccattg
6061 catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg
6121 ccatgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
6181 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
6241 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
6301 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
6361 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
6421 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
6481 tacgtattag tcatcgctat taccatg
//
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