Lenti-Hb9-GFP

LOCUS        Lenti-Hb9-GFP               12313 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  3rd generation lentiviral transfer plasmid for Hb9 GFP.
ACCESSION   .
VERSION     .
KEYWORDS    Lenti-Hb9-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12313)
  AUTHORS   Marchetto MC, Muotri AR, Mu Y, Smith AM, Cezar GG, Gage FH
  TITLE     Non-cell-autonomous effect of human SOD1 G37R astrocytes on motor 
            neurons derived from human embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2008 Dec 4;3(6):649-57.
  PUBMED    19041781
REFERENCE   2  (bases 1 to 12313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12313
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6138..6176
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             6208..6927
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             6208..6924
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6253..6274)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6514..6533)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6861..6882
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7120..7708
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7173..7193)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7591..7602)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8115..8295
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8321..8338)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8327..8551
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8597..9025
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8684..8703)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8894..8915
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9034..9054)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9039..9368
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9219..9354
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9281..9300
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9416..9463
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9482..9856
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9986..10107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10023..10042)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10077..10096
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10156..10172)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10156..10172)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10169..10191)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10180..10196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10204..10234)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10249..10270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10387..10404)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10558..11146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10638..10657)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11317..12177)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11940..11959
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12178..12282)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gatgcggccg
     2521 ctcgactcga tcctgtctcc ctggccagga gtctggggtc cgcagatata ttacaacaaa
     2581 attactaatt ggaaaatgaa attgtcaata aagcacaccc cacgcgtgta ctatatcttt
     2641 aaatattatg atgtcttatt ggtcccattt ccttacaata atttattgca ataaaacaga
     2701 cactgcaggt ccccttaaat tgtcttttca taatgaataa atttaagcag gctaattaat
     2761 atataaacta gctcaatttg tcaagttgat ttgtatttta gttaattgtg aaagtaatta
     2821 ccacatggtc aaattaacag ctttctggaa atgaccaagc ctgaggtttt atttccttcc
     2881 tgggtgaaga aaattcattt ttccaagctc ttgatgtgat gaataaaagt cataaatctg
     2941 ggtgattggt gcaggcagag tctaaatggc ttcatatttc attttaggtt taatagaaat
     3001 attcatgctc tgttttaatg aaattaaatt gaagggggat ggggctctgg aggcggccct
     3061 ggctgggggc ttaaagggac agtgcacttt tcctttcttc atgcaagggc cagggctaga
     3121 ggtccctgga ctcagtcaag aagtggagag agctgaggga ggcctgggag gcccttattc
     3181 tgtacccagt gtagcacacc tgcagccctg attggggggg ggggcggggg caattcacac
     3241 cctgtattct caccaggcta aagtttcttc tatctggttc cttctctcca aaaaatcagt
     3301 cagaagggac ctcccttggc tgagagtaga agtgaggtgg tcagcgtgct gcagacaagc
     3361 ctgtcctcca tctgcctaag gggtctgata tcctacacca agccccccct accccttctg
     3421 gagacacata ggagataagc attttcaaaa ccaccatggc actctcctgt tctcccctgc
     3481 accccttcct cccagactcc atcctgcctg aggatgattt ggtccaactt gaataataga
     3541 gtactctctt tccacatctt tccacatcgg aaagagtagt ccgggatcca gtccacctgc
     3601 agccctgatt aaaggaagga aggaagacta gaggcgcagt tctacctatt atacttattt
     3661 taaggttccc aggtcagaga tgcctcatgg acatttctcc ctaaggcagg tcctgaccag
     3721 ccctccccct gaatggtgct gatctgtgac ttgttgcttc tattgaacaa atacagcttt
     3781 gatttgtttc cagccagagt ggttagctga tgaattgaca aaaactaatc agctttattg
     3841 ggaaacaggt ttaagggcac ggacgtgtca ataacgctca gcctgacccc ctcttccatt
     3901 agctaggcag gctgattaga gtaagaagac cctctcgccg attgcaccga aattgctttc
     3961 ataaactcac aatattagca gtcgatgtga gcagctaatt tagagaagac atccaggccc
     4021 gtcacatcga tcatcgggtt tatgtatcat tatctcattt acagaatgtc gcagtgtaat
     4081 acatgttttt ggcaataggc ttggccgctc gccggagccg cgcgctgttc ctttcggcca
     4141 cactgcaggg gacttcctca acaatctacg atgtctcttc gcgtgaacaa ggaggcactt
     4201 tgcacacata tgcacacttg cgcccaggct ctcagtgcgc gctcagtgca cacatgcacg
     4261 tggacacatg ctccgacata ctacctgagc tgcctttcct tctcatgcgg ggatgctggc
     4321 attgctctct cctgcgagcc cctggtctga agcccaatgc cggacgctca ccaaccttgg
     4381 agtcaacctc ggagagctca aggttcggtt ctgtcaccag catacagaga tggtgggtgg
     4441 cggcgcaatc agccaccttc tctcaaggct ttggtcgccc agttggcggg tgtccagaaa
     4501 tccacaggcc tgcgaaggtc cttctccgcc ctcccaggct ccagcttgac ctgccctgcc
     4561 ctgggcgggc ctttctcgca acacttccag gctcagccag atgcgcctgg tcttagggag
     4621 tgggccacct gttcctttgg gtaattattt taatgtaagg ggtctacttc aggcctcctg
     4681 cagtgacaga cggcagcgga gctgcaaagc gctctcagcc tttcagtgga atttcccaga
     4741 tgggccaagg ggaggccagg gcgggtcacc aggggccctt taacgagttc attagccaaa
     4801 ttatcttgcc tgcagcaagg accccagaga ctgtagggag agtaggggag aaggtgggtc
     4861 tttctccctc cctgctagtc tgcaagaaca gttgtgggaa ggaaagagaa aggacatcct
     4921 ccccaaaggg gcctaggaac actaaaggca aagatggcat ctctgatgtg acttatgcgc
     4981 cagaccagcg aggtggtgga gtggtgggaa ggaatcataa tgattaggaa acacggagat
     5041 tctaacaacc agggcactga cccatattgt ggaacaaacc tagtgcagaa atcaaagtca
     5101 gagcccctgg aactttattc tgtgcttctt aagcaaagaa cctggacatc ttggagcctt
     5161 ggtctgtccg tccatctgta gagtagaagg taggtgtgtt tccttccaca atttgtgaga
     5221 tcactgccct aagaaaagca actctgggaa gagcttctgg catgaccctg ttgggtcctt
     5281 cgggccccct tactcctgtg gtaggaggct ttggcaggtt gaggtgagcc tgatcctaga
     5341 tgcctgtgct ggaaaatgaa cccaactctc acctgtcgtc gcgcccccca cccccacccc
     5401 acagctgtga ggcgctgctc tggaggtagc tcagcatccc cgcccctcct ccttgtcctc
     5461 actctgcctc ctgtagtgtt agcttctgct acctcagcaa gtagtgagtg aatagaccaa
     5521 ttagccagga cttcctgcca ttatctgctg tagacttggg cattagtacg ccctgattca
     5581 ccataatttc aagtcagaac acactaaaaa attcactcac cgaggcatta aaatgttccc
     5641 agacaacgtc tgtgtttgtg taactgggtt tcttcccttc gtgtttgatg cacaccagca
     5701 tcatataggt cagtggtgga gtccaggcta ggcagacaat ttttgtcgct ttttgtttgt
     5761 ttttatattt ttaaaacatg aattgcattg tttcccggtg gttgtaaact ttgtttctgg
     5821 ggcttcagta ttgccagtgt aatcttcttc tgcttgatca ggggcaaaca agtgacttag
     5881 aaagttgtgg gagtgcccta agaacagtag ccagagcctt tacagagcca ggctttgtgt
     5941 cctaaacgta catcgcccca gctatgggaa gctgggttat caccttcaaa gcagaccctt
     6001 tctgtggatg acttaccaag ccctgcaggt gggtagtgga ggtggggaga gggctgcaca
     6061 gcctgcttgg agcttgcttc tgttctttgt cctgcccatg atatgaagct cctatacaat
     6121 gaatttgcag taagatcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
     6181 tagtgaaccg cagatccggc gcgccccatg gtgagcaagg gcgaggagct gttcaccggg
     6241 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     6301 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     6361 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     6421 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     6481 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     6541 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     6601 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     6661 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     6721 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     6781 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     6841 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     6901 ctcggcatgg acgagctgta caagtaaagc ggccaattcg gatccgttta aactctagag
     6961 gatccaccgg tggccgcctg ggcccgttaa cgtctcgagt ttaaacctgg aaaaacatgg
     7021 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     7081 agaggaggag gaggtgggtt ttccagtcac acctcagaca atcaacctct ggattacaaa
     7141 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     7201 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7261 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7321 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7381 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7441 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7501 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     7561 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7621 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7681 cggatctccc tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa
     7741 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7801 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     7861 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     7921 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     7981 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     8041 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
     8101 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8161 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8221 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8281 ggaaaatctc tagcagggcc gctttaaacc cgctgatcag cctcgactgt gccttctagt
     8341 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     8401 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     8461 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     8521 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     8581 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     8641 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     8701 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     8761 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     8821 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     8881 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     8941 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     9001 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     9061 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     9121 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     9181 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     9241 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     9301 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     9361 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     9421 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     9481 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     9541 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     9601 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     9661 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     9721 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     9781 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     9841 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     9901 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     9961 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
    10021 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10081 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
    10141 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    10201 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    10261 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    10321 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    10381 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    10441 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    10501 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    10561 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    10621 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    10681 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    10741 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    10801 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    10861 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    10921 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    10981 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    11041 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    11101 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    11161 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    11221 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    11281 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    11341 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    11401 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    11461 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    11521 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    11581 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    11641 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    11701 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    11761 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    11821 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    11881 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    11941 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    12001 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    12061 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    12121 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    12181 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    12241 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    12301 gccacctgac gtc
//