pFastBacHT B

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pFastBacHTB载体基本信息

出品公司: Invitrogen
载体名称: pFastBacHTB, pFastBac HTB, pFastBacHT B, pFastBac HT B
质粒类型: 昆虫细胞表达载体;杆状病毒表达载体
启动子: Polyhedrin
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4857 bp
5' 测序引物: pFastbac-F: TATTCCGGATTATTCATACC
3' 测序引物: pFastBac-R: ACAAATGTGGTATGGCTGA
载体标签: His tag (N-末端)
载体抗性: Ampicillin 氨苄
筛选标记: Gentamicin Gentamycin_2.html' target='_blank'>庆大霉素
备注:
--
产品目录号: 10584-027
稳定性: 稳表达 Stable
组成型: 组成型 Constitutive
病毒/非病毒: 杆状病毒

pFastBacHTB载体质粒图谱和多克隆位点信息

pFastBacHT B 载体图谱



pFastBacHT B 多克隆位点

pFastBacHT B 载体特征

pFastBacHTB载体简介

Bac-to-BacHT Vector Kit 设计用于在大肠杆菌 (E. coli) 中生成杆状病毒载体后,在 Sf9、Sf21 或 High Five 细胞中表达和纯化带组氨酸标签的重组蛋白。 pFastBacHT 载体具有以下特点:

 用于表达蛋白质的多角体强启动子

 用于简化克隆的三个读码框

 用于轻松纯化重组融合蛋白的 N-端 6xHis 标签

 用于在蛋白质纯化后去除组氨酸标签的 TEV 蛋白酶切割位点

引用及参考文献

DEF6, a Novel Substrate for the Tec Kinase ITK, Contains a Glutamine-rich Aggregation-prone Region and Forms Cytoplasmic Granules that Co-localize with P-bodies.
Hey F, Czyzewicz N, Jones P, Sablitzky F, 
J Biol Chem (2012) 287:31073-31084
Product usage: na

Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin.
Dan C; Kelly A; Bernard O; Minden A; 
J Biol Chem (2001) 276:32115-32136
Product usage: pFASTBAC/HT and pFASTBAC-1 vectors were used to transfect with Sf9 cells with Cellfectin.

Sp3 Represses Gene Expression via the Titration of Promoter-specific Transcription Factors.
Kennett Sarah B; Moorefield K Scott; Horowitz Jonathan M; 
J Biol Chem (2002) 277:9780-9789
Product usage: Baculovirus stocks encoding Sp1, Sp3, M1, and M2 were prepared using the PCR, appropriate primers, and pCMV4-Sp1/flu, pBSK-Sp3/flu, pCR-M1/flu, 
and pCR-M2/flu as substrates. PCR reactions employed pGEX-M2 using Taq polymerase. Amplified cDNAs were subcloned in pFASTBacHTA.

pFastBacHTB载体序列

LOCUS       pFastBacTHT\B           4857 bp    DNA     circular SYN  2-SEP-2003
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4857
                     /invitrogen="607"
                     /vntifkey="98"
     CDS             589..1449
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     promoter        490..588
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     rep_origin      2..457
                     /invitrogen="2520000"
                     /vntifkey="33"
                     /label=f1\origin
     CDS             complement(2802..3335)
                     /invitrogen="1150000"
                     /vntifkey="4"
                     /label=Gm(R)
     misc_feature    4119..4223
                     /invitrogen="1530000"
                     /vntifkey="21"
                     /label=MCS
     promoter        complement(3524..3551)
                     /invitrogen="2250000"
                     /vntifkey="29"
                     /label=Pc\promoter
     promoter        3904..4032
                     /invitrogen="2260000"
                     /vntifkey="29"
                     /label=PH\promoter
     primer          3920..3937
                     /invitrogen="2060000"
                     /vntifkey="27"
                     /label=Polyhedrin\forward\primer
     rep_origin      1594..2267
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     polyA_signal    4241..4481
                     /invitrogen="1850000"
                     /vntifkey="25"
                     /label=SV40\pA
     CDS             4101..4121
                     /invitrogen="1300000"
                     /vntifkey="4"
                     /label=TEV\recognition\site
     misc_recomb     4500..4694
                     /invitrogen="2750000"
                     /vntifkey="86"
                     /label=Tn7L
     misc_recomb     2512..2735
                     /invitrogen="2760000"
                     /vntifkey="86"
                     /label=Tn7R
     CDS             4062..4079
                     /invitrogen="1015000"
                     /vntifkey="4"
                     /label=6xHis
     CDS             4050..4052
                     /invitrogen="123000000"
                     /vntifkey="4"
                     /label=initiation\ATG
BASE COUNT     1219 a      1198 c      1152 g      1288 t
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     4021 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg
     4081 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatggga tccggaattc
     4141 aaaggcctac gtcgacgagc tcactagtcg cggccgcttt cgaatctaga gcctgcagtc
     4201 tcgaggcatg cggtaccaag cttgtcgaga agtactagag gatcataatc agccatacca
     4261 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     4321 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     4381 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4441 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg atatcgccta
     4501 ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc
     4561 gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat
     4621 ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagcggg
     4681 gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg tgacaaaccg
     4741 tcatcttcgg ctactttttc tctgtcacag aatgaaaatt tttctgtcat ctcttcgtta
     4801 ttaatgtttg taattgactg aatatcaacg cttatttgca gcctgaatgg cgaatgg
//

pFastBacHTB载体质粒图谱pdf版和相关资料下载

pFastBacHTB载体应用举例

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