pcDNA3.1/Hygro(+)载体图谱质粒图谱、序列、价格、抗性、测序引物、大小等基本信息

出品公司:	Invitrogen
载体名称:	pcDNA3.1/Hygro(+)
质粒类型:	哺乳动物细胞表达载体
启动子:	CMV
表达水平:	高
克隆方法:	多克隆位点，限制性内切酶
载体大小:	5597 bp
5' 测序引物:	pcDNA3.1-F:CTAGAGAACCCACTGCTTAC
3' 测序引物:	pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG
载体标签:	C-His, C-Myc
载体抗性:	Ampicillin 氨苄
筛选标记:	Hygromycin 潮霉素
备注:	Differs from other pcDNA3.1 in drug resistance; +/- refers to orientation of f1 ori.
产品目录号:	V870-20
稳定性:	瞬表达 Transient
组成型:	组成型 Constitutive
病毒/非病毒:	非病毒

pcDNA3.1/Hygro(+)载体图谱

pcDNA3.1/Hygro (+) and pcDNA3.1/Hygro (–) are 5.6 kb vectors derived from pcDNA3.1 and are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. These vectors contain the following elements:
•Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells
•Multiple cloning sites in the forward (+) and reverse (–) orientations to facilitate cloning
•Hygromycin resistance gene for selection of stable cell lines
•Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g., COS–1, COS–7)
A control plasmid, pcDNA3.1/Hygro/lacZ, is included for use as a positive control for transfection and expression in the cell line of choice.

LOCUS       pcDNA3.1/Hygro(+)   5597 bp    DNA   SYN
DEFINITION pcDNA3.1/Hygro(+)
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="pcDNA3.1/Hygro(+)"
                     /mol_type="other DNA"
     promoter        236..852
                     /label="CMV_immearly_promoter"
     misc_feature    315..602
                     /label="CAG_enhancer"
     misc_feature    769..789
                     /label="CMV_fwd_primer"
     promoter        863..881
                     /label="T7_promoter"
     misc_feature    complement(1022..1039)
                     /label="BGH_rev_primer"
     terminator      1025..1252
                     /label="bGH_PA_terminator"
     rep_origin      1315..1621
                     /label="f1_origin"
     misc_feature    complement(1735..1755)
                     /label="pBABE_3_primer"
     misc_feature    complement(1741..1956)
                     /label="SV40_enhancer"
     promoter        1753..2021
                     /label="SV40_promoter"
     rep_origin      1920..1997
                     /label="SV40_origin"
     misc_feature    1982..2001
                     /label="SV40pro_F_primer"
     CDS             complement(2055..2876)
                     /label="ORF frame 1"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHHVLIRSENGYTSSRELFAKA*"
     CDS             2118..3143
                     /label="ORF frame 3"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     gene            2133..3140
                     /label="hygroB"
                     /gene="hygroB"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     terminator      3276..3395
                     /label="SV40_PA_terminator"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     misc_feature    3364..3383
                     /label="EBV_rev_primer"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     promoter        complement(3439..3457)
                     /label="M13_reverse_primer"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     misc_feature    complement(3456..3478)
                     /label="M13_pUC_rev_primer"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     promoter        complement(3492..3521)
                     /label="lac_promoter"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     gene            complement(4601..5461)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     CDS             complement(4601..5461)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5503..5531)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG
   61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG
121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC
181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT
241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA
301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC
361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC
421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT
481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT
541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA
601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG
661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC
721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG
781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA
841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC
901 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT GGAATTCTGC
961 AGATATCCAG CACAGTGGCG GCCGCTCGAG TCTAGAGGGC CCGTTTAAAC CCGCTGATCA
1021 GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC
1081 TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG
1141 CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG
1201 GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG
1261 GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA
1321 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG
1381 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA
1441 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC
1501 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT
1561 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA
1621 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC
1681 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG
1741 TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA
1801 TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA
1861 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA
1921 TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT
1981 TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG
2041 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG
2101 GATCTGATCA GCACGTGATG AAAAAGCCTG AACTCACCGC GACGTCTGTC GAGAAGTTTC
2161 TGATCGAAAA GTTCGACAGC GTCTCCGACC TGATGCAGCT CTCGGAGGGC GAAGAATCTC
2221 GTGCTTTCAG CTTCGATGTA GGAGGGCGTG GATATGTCCT GCGGGTAAAT AGCTGCGCCG
2281 ATGGTTTCTA CAAAGATCGT TATGTTTATC GGCACTTTGC ATCGGCCGCG CTCCCGATTC
2341 CGGAAGTGCT TGACATTGGG GAATTCAGCG AGAGCCTGAC CTATTGCATC TCCCGCCGTG
2401 CACAGGGTGT CACGTTGCAA GACCTGCCTG AAACCGAACT GCCCGCTGTT CTGCAGCCGG
2461 TCGCGGAGGC CATGGATGCG ATCGCTGCGG CCGATCTTAG CCAGACGAGC GGGTTCGGCC
2521 CATTCGGACC GCAAGGAATC GGTCAATACA CTACATGGCG TGATTTCATA TGCGCGATTG
2581 CTGATCCCCA TGTGTATCAC TGGCAAACTG TGATGGACGA CACCGTCAGT GCGTCCGTCG
2641 CGCAGGCTCT CGATGAGCTG ATGCTTTGGG CCGAGGACTG CCCCGAAGTC CGGCACCTCG
2701 TGCACGCGGA TTTCGGCTCC AACAATGTCC TGACGGACAA TGGCCGCATA ACAGCGGTCA
2761 TTGACTGGAG CGAGGCGATG TTCGGGGATT CCCAATACGA GGTCGCCAAC ATCTTCTTCT
2821 GGAGGCCGTG GTTGGCTTGT ATGGAGCAGC AGACGCGCTA CTTCGAGCGG AGGCATCCGG
2881 AGCTTGCAGG ATCGCCGCGG CTCCGGGCGT ATATGCTCCG CATTGGTCTT GACCAACTCT
2941 ATCAGAGCTT GGTTGACGGC AATTTCGATG ATGCAGCTTG GGCGCAGGGT CGATGCGACG
3001 CAATCGTCCG ATCCGGAGCC GGGACTGTCG GGCGTACACA AATCGCCCGC AGAAGCGCGG
3061 CCGTCTGGAC CGATGGCTGT GTAGAAGTAC TCGCCGATAG TGGAAACCGA CGCCCCAGCA
3121 CTCGTCCGAG GGCAAAGGAA TAGCACGTGC TACGAGATTT CGATTCCACC GCCGCCTTCT
3181 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG
3241 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT
3301 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA
3361 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA
3421 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA
3481 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG
3541 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT
3601 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC
3661 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG
3721 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA
3781 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
3841 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA
3901 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG
3961 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG
4021 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC
4081 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
4141 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA
4201 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT
4261 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG
4321 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG
4381 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT
4441 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG
4501 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA
4561 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG
4621 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG
4681 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC
4741 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG
4801 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG
4861 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG
4921 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT
4981 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC
5041 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC
5101 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA
5161 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC
5221 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT
5281 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC
5341 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA
5401 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA
5461 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT
5521 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA
5581 AAGTGCCACC TGACGTC
//

pCambia1201	pJW342
pMV261	pTCK303
pCMV-3Tag-3a	pCAMBIA3200
pDEST10	pcDNA4/V5-His B
pCR4-35	pIJ702-87del(78-86, 88-132)
pHIC-PI	pTALETF_v2 (NG)
pcDNA6.2/V5-PL-DEST	pGFP22
pTrcHis2 C	pLVX-Myc-MCS-Strep-IRES-Puro
pcDNA3.1/His B	pFtsH_TA
pFB-hrGFP	pGL4.39[luc2P/ATF6 RE/Hygro]
pTT5	pPZP122
pGL101	pG5luc
pCTAP-Shuttle-A	pNIT-3
pSW510	pSinRep5
pCB1535	pTYB2
pMSCG19	pECFP-Nuc
pYES2-EGFP	pBHR68
pFastBac Dual	pCas-Guide-EF1a-GFP
pFastBac1	pDP5rs
pB-gyrase_TA	pHC79-2cos/tk
pGL4.77[hRlucP/Hygro]	pFA1
pKO11	p53-Luc
pAc5.1-V5-His A	pAX01-ComK
pCMV5	pOS1
pFastBac-GST-C	pSilencer5.1-H1 Retro
pET-32 EK/LIC	pXIS11
pQE-32	pMIB/V5-His A
pLenti CMV/TO Neo empty (w215-1)	pcDNA3.1+/N-GST(TEV)
pacAd5 9.2-100	IR2
pcDNA3.1+P2A-eGFP	pKLAC2
pRS426	pMMB33
pCold-GST	pWW60-1
pGL4.83[hRlucP/Puro]	pBad/Myc-His A
pLVX-rHom-Sec1	pET-12c(+)
VaD1	pME12::Tn5-259
pBApo-CMV-Pur	pDR540
pUCDM	p3xFLAG-KV2.1
pCas	pET-28a-EGFP
pGL4.25[luc2CP/minP]	pVW5JI
pEF1/His B	phCMV1
pPH3	pIB/V5-His
pcDNA4/HisMax A	pCDF1-MCS2-EF1-Puro
pMKO.1-GFP	pAc5.1a
pEF4/His B	pYES2-kan
pZeoSV2 (+)	pBT-5
pLKO-DEST-EGFP	PX391
pSRE-Luc	pIAβ8
pMYs-IRES-GFP	pFN31K Nluc-CMV-neo-Flexi
pE	DR2
pSC101 and pKG2	pET302/NT-His

pJQ200mp18	pHAGE2-TetOminiCMV-SKM
pNI3	pRJPaph-bjGFP
pGL4-ERSE2-luc2P-Hygro	pGL3 2 kb prom. CD274
pTK299	pCMV-SynA
Tcf/Lef:H2B-GFP	pEJS654 All-in-One AAV-sgRNA-hNmeCas9
pmGFP	CaV1.2
BCR/ABL P190 transgenic construct	pET21a-BirA
pCas9	pcDNA3.1 myc-NL-GW
Stat3 Flag pRc/CMV	EK0285 SFFV-mCherry-SG(s70587)SG-EGFP (F
pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-V	pLentipuro3/TO/V5-GW/EGFP-Firefly Lucife
pSBbi-GN	pBAD18-Cm
pCMVR8.74	p415-GalL-Cas9-CYC1t
pBabe-hygro PyMT	pCMVInt
pRK5-p18-HA	pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T
xCas9(3.6)-BE3	sfGFP-Cx43K258stop
pMSCV-FlagMLL-pl-ENL(5613)	pGM1202
pHIV-iRFP720-E2A-Luc	pPBbsr2-4031NES
pcDNA3/Flag-METTL3	pRN11
pPH37	pGIPZ-PD-L1-EGFP
pCMV-ABE7.9	MSP2135
pBS-hBAF60a	pC0061 PsmCas13 (B05) His6-TwinStrep-SUM
pFE-sfGFP	pSELECT-HA-mFOXO1
pRK GFP Paxillin	pLV-puro-N-3HA-SREBP1-C-Flag
DNMT3b KO Hyg	pCMV-HsPeg10rc3
Flag-TRIM24	jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcode
pBabe-puro-miR-10b sponge	BECLIN-HA WT
pCRISPR-aBEST	pTNT-B18R-6His
pF151 pcDNA3.1(+)SOD1WT	pCMV-2NLS-Cas9-6XMS2
pGL3-mArg1 promoter/enhancer -31/-3810	pDA077
pcDNA3.1-mCherry	pSIN4-EF1a-LMO2-IRES-Puro
pHAGE NFkB-TA-LUC-UBC-GFP-W	pMDIAI
pCAS9-TPC	pc DNA FRT TO- APPSwed/Ind
pminiTol2	hCas9_D10A
pNLF1W	pCI neo-hEST2
pMXs-hc-MYC	PG13-luc (wt p53 binding sites)
pET15b_Bst-LF	pAAV-EF1a-DIO-mCherry
pCMVHA hEZH2	FUGW-PercevalHR
pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2	pLenti PGK Puro DEST (w529-2)
pYLCRISPR/Cas9pUbi-N	pUCmini-iCAP-AAV.CAP-B22
pR26 GFP Dest	pY001 (pFnCpf1_full)
pMaster1	MSP680
Plasmid L5 attB::Pleft* mCherry	pEcgRNA
LentiGuide Cherry	TrkC-GFP
pHyo094 (eMutaT7)	pHAtC
mTert-pBabe-puro	pHAGE2-TetOminiCMV-mCherry
SIRT3 Flag	pBECKP-km
pmScarlet_Giantin_C1	pEGFP-NMHC II-C
pBFC1207	pCAG Cas9-2A-Citrine
pHIV-Luc-ZsGreen	cytoplasmic EKAR (Cerulean-Venus)
mTagBFP2-TOMM20-N-10	pAAV-EF1a-DIO-HTB

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