| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-BRWD3-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001667 |
| 产品用途: | 3' AAVLINK plasmid for BRWD3 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | BRWD3 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-BRWD3-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-BRWD3-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-BRWD3-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-BRWD3-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-BRWD3-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-BRWD3-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-BRWD3-FLAG 6794 bp DNA circular SYN 22-JUL-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6794)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6794
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
misc_feature 156..236
/label=SCP1
CDS 248..1276
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1292..1381
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1306..1311
/label=SDS
misc_feature 1309..1314
/label=SDS
intron 1385..1425
/label=Splicing Donor
protein_bind 1432..1465
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1466..1557
/label=Splicing Acceptor
/note="Splicing acceptor"
misc_feature 1558..3507
/label=BRWD3
misc_feature 1904..1909
/label=SDS
misc_feature 3111..3116
/label=SDS
misc_feature 3153..3158
/label=SDS
misc_feature 3168..3173
/label=SDS
misc_feature 3234..3239
/label=SDS
misc_feature 3361..3366
/label=SDS
CDS 3514..3579
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3583..4014
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3727..3732
/label=SDS
repeat_region 4057..4197
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4057..4186
/label=AAV2 ITR
rep_origin 4272..4727
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5009..5113
/gene="bla"
/label=AmpR promoter
CDS 5114..5974
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6145..6733
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtgtact tatataaggg ggtgggggcg
181 cgttcgtcct cagtcgcgat cgaacactcg agccgagcag acgtgcctac ggaccgcgta
241 cgccaccatg tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc
301 aacgagtgat gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc
361 tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt
421 gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct
481 tcaggcgcgc ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct
541 tcatcgtcgg tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg
601 gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga
661 acgcactgat ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat
721 acgtaatctg gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc
781 caggatcagg gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg
841 cagaacgaaa acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac
901 taaactggtc gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct
961 gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac
1021 tcgcgccctg gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga
1081 ctctggtcag agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga
1141 tatggcccgc gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt
1201 aaatattgta atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct
1261 gctggaagat ggcgatggag gtggatctgg tctgctgctg aaactcctcc tcctcggtgg
1321 tggttctggt cctcgtcccc gtggtactcg tggtaaaggt cgccgtattc actacaaagg
1381 ataagtaagt atcaaggtta caagacaggt ttaaggagac caataactag tataacttcg
1441 tataggatac tttatagcaa tttatgaaac tgggcttgtc gagacagaga agactcttgc
1501 gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc tccacagggc
1561 atcaaccacc ttctttccct ggattttgcc agcccttttg ctgttccagt ggatctcagt
1621 gcctacccct tgtattgtac tgtagttgct tatccaactg acctcaatac catcaggcgg
1681 agacttgaaa atcgctttta caggagaata tcagcattaa tgtgggaggt acgctatatt
1741 gaacataatg ccaggacttt caatgagcca gacagtccta tagttaaagc agctaaaatt
1801 gtaactgatg tcttacttcg atttattggg gatcagagct gtactgatat actggatact
1861 tataataaaa ttaaagcaga agaacgaaac agtactgatg cagaggagga tacagaaatt
1921 gttgatttag attcagacgg tcctggtact tcatctggaa gaaaggtcaa atgccgaggc
1981 agaagacagt ctttaaagtg taatcctgat gcttggaaaa aacaatgcaa ggaactattg
2041 agcctcattt atgaacgtga agactcggag ccatttcgac agccagctga tcttctttct
2101 tacccaggtc atcaggagca agagggagaa tcctcagagt ctgttgttcc agaaagacaa
2161 caagattcat ctctttctga ggattatcaa gatgttatag atactcctgt ggacttcagc
2221 actgtgaaag aaactttgga agcaggaaac tatggtagtc ctctggaatt ttataaggat
2281 gttcgccaaa tattcaacaa ctccaaagct tatacctcta ataaaaagtc aaggatctat
2341 agcatgatgc tgcgattatc tgccttattt gaaagtcata tcaaaaatat catctctgaa
2401 tataagtcag caatccagag tcagaagagg agaaggccac ggtacagaaa acgtctaaga
2461 agcagcagca gttcattatc tagtagtgga gcacctagtc caaaagggaa gcagaaacaa
2521 atgaagttgc agcctaaaaa tgaccagaat acctcagtat ctcatgccag gactagctct
2581 cctttctcat ctcctgtttc agatgctgct gaagggcttt cactatatct acttgatgat
2641 gagccagatg ggccattttc ttcatcgagc ttcggtggat atagccgaag tggaaatagc
2701 catgacccag ggaaagccaa atcatttcgg aatagagttt tgccagttaa acaagatcac
2761 tcccttgatg gaccccttac aaatggtgat ggcagagagc cccggacagg aatcaagaga
2821 aaactactta gtgcatcaga agaagatgaa aacatgggag gagaagataa agagaaaaaa
2881 gaaacaaaag agaaatctca tttatccacc tcagagagtg gagagttagg atccagttta
2941 agttctgaaa gtacctgtgg ttctgactct gactctgaat caacttccag aacagatcaa
3001 gattacgtag atggagacca tgattatagc aaattcatac aaaccagacc taaaagaaaa
3061 ctcagaaagc aacatggcaa tggaaaaaga aattggaaga ccagaggcac aggaggaaga
3121 ggcagatggg gaagatgggg tagatggagt agaggaggca gaggaagagg aggcagggga
3181 cgagggagtc gaggaagagg tggaggtggc actaggggga ggggaagagg gagaggagga
3241 agaggtgctt ctagaggagc taccagagcc aaacgagcac gtattgcaga tgatgaattt
3301 gataccatgt tttcaggacg tttcagtaga ctgcctcgaa ttaaaacaag aaaccaaggc
3361 aggaggactg ttttatataa tgatgattct gataatgaca attttgtgtc cactgaggat
3421 cctctgaatc ttggtacatc cagatcaggc agagtgcgta aaatgacaga aaaagccagg
3481 gttagccatc tcatgggatg gaattattgt acagactaca aagaccatga cggtgattat
3541 aaagatcatg acatcgacta caaggatgac gatgacaagt aactcgagat aatcaacctc
3601 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
3661 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
3721 ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc atcgccgcct
3781 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttta
3841 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat ctagctttat ttgtgaaatt
3901 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
3961 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagatct
4021 ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
4081 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
4141 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
4201 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
4261 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
4321 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
4381 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
4441 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
4501 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
4561 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc
4621 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
4681 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
4741 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
4801 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
4861 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
4921 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
4981 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
5041 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
5101 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
5161 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
5221 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
5281 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
5341 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
5401 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
5461 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
5521 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
5581 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
5641 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
5701 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
5761 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
5821 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
5881 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
5941 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
6001 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
6061 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
6121 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
6181 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
6241 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
6301 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
6361 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
6421 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
6481 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
6541 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
6601 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
6661 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
6721 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
6781 ttttgctcac atgt
//
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