pUC-AdEvolve-DEST

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pUC-AdEvolve-DEST
出品公司: Addgene
目录编号: 131692
存储实验室: Matthew D. Shoulders

载体骨架基本信息
载体名称: pAd⁄CMV⁄V5-DEST
载体出品公司: Thermo Fisher Scientific
空载体大小: 36831 bp
完整质粒大小: 36836 bp
载体类型: Adenoviral
载体抗性: 氨苄霉素
生长温度: 37 ℃
宿主菌: DH10B gyrA462
拷贝数: 高拷贝

插入基因信息
克隆方法: Gateway Cloning

文献引用方法
材料和方法部分: pUC-AdEvolve-DEST was a gift from Matthew D. Shoulders (Addgene plasmid # 131692 ; http://n2t.net/addgene:131692 ; RRID:Addgene_131692)
参考文献部分: An Adaptable Platform for Directed Evolution in Human Cells. Berman CM, Papa LJ 3rd, Hendel SJ, Moore CL, Suen PH, Weickhardt AF, Doan ND, Kumar CM, Uil TG, Butty VL, Hoeben RC, Shoulders MD. J Am Chem Soc. 2018 Dec 26;140(51):18093-18103. doi: 10.1021/jacs.8b10937. Epub 2018 Dec 14. 10.1021/jacs.8b10937 PubMed 30427676

载体图谱质粒图谱和多克隆位点信息

pUC-AdEvolve-DEST载体图谱

载体简介

载体序列

LOCUS       pUC-AdEvolve-DEST              36836 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  A version of AdEvolve-DEST with a high copy origin for easier DNA 
            preparation and transfection..
ACCESSION   .
VERSION     .
KEYWORDS    pUC-AdEvolve-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 36836)
  AUTHORS   Berman CM, Papa LJ 3rd, Hendel SJ, Moore CL, Suen PH, Weickhardt AF,
            Doan ND, Kumar CM, Uil TG, Butty VL, Hoeben RC, Shoulders MD
  TITLE     An Adaptable Platform for Directed Evolution in Human Cells.
  JOURNAL   J Am Chem Soc. 2018 Dec 26;140(51):18093-18103. doi: 
            10.1021/jacs.8b10937. Epub 2018 Dec 14.
  PUBMED    30427676
REFERENCE   2  (bases 1 to 36836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..36836
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     polyA_signal    347..571
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     560..577
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(600..617)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(627..668)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    694..818
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(859..1164)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(961..980)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(1506..2165)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2080..2099
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2219..2249)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             complement(2312..3115)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2423..2442)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3033..3052
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3134..3181)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    complement(3206..3330)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3359..3378)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3360..3378)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3423..3626)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3452..3472)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3627..4006
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    7542..7645
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     CDS             complement(14168..14179)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(33205..33225)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        33210..33539
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      33390..33525
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     33452..33471
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             33560..34270
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(33705..33723)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     33973..33992
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    34430..34551
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(34467..34486)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     34521..34540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   34647..34749
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      complement(34973..35561)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(35053..35072)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(35713..36573)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     36336..36355
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(36574..36678)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     36746..36764
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tagaagccat agagcccacc
      361 gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc
      421 ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat
      481 taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa
      541 acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgggt ttaaactcaa
      601 tggtgatggt gatgatgacc ggtacgcgta gaatcgagac cgaggagagg gttagggata
      661 ggcttacctt cgaaccgcgg gccctctaga tcaaccactt tgtacaagaa agctgaacga
      721 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
      781 acataatact gtaaaacaca acatatccag tcactatggt cgacctgcag actggctgtg
      841 tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc
      901 attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc
      961 catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc
     1021 acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc
     1081 gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt
     1141 ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca aaagagccgt
     1201 tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt
     1261 cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga
     1321 catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg
     1381 cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata tattcttata
     1441 ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gccggatcca
     1501 gatctttacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     1561 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     1621 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     1681 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     1741 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1801 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     1861 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     1921 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga
     1981 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     2041 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     2101 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     2161 tccattttag cttccttagc tcctgaaaat ctcgacggat cctaactcaa aatccacaca
     2221 ttatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgcggc cgcgtcgagg
     2281 ctgatcagcg agctctagag aattgatccc ctcagaagaa ctcgtcaaga aggcgataga
     2341 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
     2401 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
     2461 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
     2521 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc atcgccgtcg ggcatgcgcg
     2581 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
     2641 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
     2701 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
     2761 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
     2821 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
     2881 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg
     2941 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
     3001 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca
     3061 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatggccgat cccatggttt
     3121 agttcctcac cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa
     3181 caggctgcag gtcgaaagcg gccgccatag tgactggata tgttgtgttt tacagtatta
     3241 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
     3301 ttctcgttca gcttttttgt acaaacttgt tgatagctta actagccagc ttgggtctcc
     3361 ctatagtgag tcgtattaat ttcgataagc cagtaagcag tgggttctct agttagccag
     3421 agagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
     3481 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
     3541 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
     3601 ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac
     3661 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
     3721 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
     3781 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
     3841 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
     3901 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat
     3961 gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcaacg cgtatatctg
     4021 tgggcgtggc ttaagggtgg gaaagaatat ataaggtggg ggtcttatgt agttttgtat
     4081 ctgttttgca gcagccgccg ccgccatgag caccaactcg tttgatggaa gcattgtgag
     4141 ctcatatttg acaacgcgca tgcccccatg ggccggggtg cgtcagaatg tgatgggctc
     4201 cagcattgat ggtcgccccg tcctgcccgc aaactctact accttgacct acgagaccgt
     4261 gtctggaacg ccgttggaga ctgcagcctc cgccgccgct tcagccgctg cagccaccgc
     4321 ccgcgggatt gtgactgact ttgctttcct gagcccgctt gcaagcagtg cagcttcccg
     4381 ttcatccgcc cgcgatgaca agttgacggc tcttttggca caattggatt ctttgacccg
     4441 ggaacttaat gtcgtttctc agcagctgtt ggatctgcgc cagcaggttt ctgccctgaa
     4501 ggcttcctcc cctcccaatg cggtttaaaa cataaataaa aaaccagact ctgtttggat
     4561 ttggatcaag caagtgtctt gctgtcttta tttaggggtt ttgcgcgcgc ggtaggcccg
     4621 ggaccagcgg tctcggtcgt tgagggtcct gtgtattttt tccaggacgt ggtaaaggtg
     4681 actctggatg ttcagataca tgggcataag cccgtctctg gggtggaggt agcaccactg
     4741 cagagcttca tgctgcgggg tggtgttgta gatgatccag tcgtagcagg agcgctgggc
     4801 gtggtgccta aaaatgtctt tcagtagcaa gctgattgcc aggggcaggc ccttggtgta
     4861 agtgtttaca aagcggttaa gctgggatgg gtgcatacgt ggggatatga gatgcatctt
     4921 ggactgtatt tttaggttgg ctatgttccc agccatatcc ctccggggat tcatgttgtg
     4981 cagaaccacc agcacagtgt atccggtgca cttgggaaat ttgtcatgta gcttagaagg
     5041 aaatgcgtgg aagaacttgg agacgccctt gtgacctcca agattttcca tgcattcgtc
     5101 cataatgatg gcaatgggcc cacgggcggc ggcctgggcg aagatatttc tgggatcact
     5161 aacgtcatag ttgtgttcca ggatgagatc gtcataggcc atttttacaa agcgcgggcg
     5221 gagggtgcca gactgcggta taatggttcc atccggccca ggggcgtagt taccctcaca
     5281 gatttgcatt tcccacgctt tgagttcaga tggggggatc atgtctacct gcggggcgat
     5341 gaagaaaacg gtttccgggg taggggagat cagctgggaa gaaagcaggt tcctgagcag
     5401 ctgcgactta ccgcagccgg tgggcccgta aatcacacct attaccggct gcaactggta
     5461 gttaagagag ctgcagctgc cgtcatccct gagcaggggg gccacttcgt taagcatgtc
     5521 cctgactcgc atgttttccc tgaccaaatc cgccagaagg cgctcgccgc ccagcgatag
     5581 cagttcttgc aaggaagcaa agtttttcaa cggtttgaga ccgtccgccg taggcatgct
     5641 tttgagcgtt tgaccaagca gttccaggcg gtcccacagc tcggtcacct gctctacggc
     5701 atctcgatcc agcatatctc ctcgtttcgc gggttggggc ggctttcgct gtacggcagt
     5761 agtcggtgct cgtccagacg ggccagggtc atgtctttcc acgggcgcag ggtcctcgtc
     5821 agcgtagtct gggtcacggt gaaggggtgc gctccgggct gcgcgctggc cagggtgcgc
     5881 ttgaggctgg tcctgctggt gctgaagcgc tgccggtctt cgccctgcgc gtcggccagg
     5941 tagcatttga ccatggtgtc atagtccagc ccctccgcgg cgtggccctt ggcgcgcagc
     6001 ttgcccttgg aggaggcgcc gcacgagggg cagtgcagac ttttgagggc gtagagcttg
     6061 ggcgcgagaa ataccgattc cggggagtag gcatccgcgc cgcaggcccc gcagacggtc
     6121 tcgcattcca cgagccaggt gagctctggc cgttcggggt caaaaaccag gtttccccca
     6181 tgctttttga tgcgtttctt acctctggtt tccatgagcc ggtgtccacg ctcggtgacg
     6241 aaaaggctgt ccgtgtcccc gtatacagac ttgagaggcc tgtcctcgag cggtgttccg
     6301 cggtcctcct cgtatagaaa ctcggaccac tctgagacaa aggctcgcgt ccaggccagc
     6361 acgaaggagg ctaagtggga ggggtagcgg tcgttgtcca ctagggggtc cactcgctcc
     6421 agggtgtgaa gacacatgtc gccctcttcg gcatcaagga aggtgattgg tttgtaggtg
     6481 taggccacgt gaccgggtgt tcctgaaggg gggctataaa agggggtggg ggcgcgttcg
     6541 tcctcactct cttccgcatc gctgtctgcg agggccagct gttggggtga gtactccctc
     6601 tgaaaagcgg gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg
     6661 atattcacct ggcccgcggt gatgcctttg agggtggccg catccatctg gtcagaaaag
     6721 acaatctttt tgttgtcaag cttggtggca aacgacccgt agagggcgtt ggacagcaac
     6781 ttggcgatgg agcgcagggt ttggtttttg tcgcgatcgg cgcgctcctt ggccgcgatg
     6841 tttagctgca cgtattcgcg cgcaacgcac cgccattcgg gaaagacggt ggtgcgctcg
     6901 tcgggcacca ggtgcacgcg ccaaccgcgg ttgtgcaggg tgacaaggtc aacgctggtg
     6961 gctacctctc cgcgtaggcg ctcgttggtc cagcagaggc ggccgccctt gcgcgagcag
     7021 aatggcggta gggggtctag ctgcgtctcg tccggggggt ctgcgtccac ggtaaagacc
     7081 ccgggcagca ggcgcgcgtc gaagtagtct atcttgcatc cttgcaagtc tagcgcctgc
     7141 tgccatgcgc gggcggcaag cgcgcgctcg tatgggttga gtgggggacc ccatggcatg
     7201 gggtgggtga gcgcggaggc gtacatgccg caaatgtcgt aaacgtagag gggctctctg
     7261 agtattccaa gatatgtagg gtagcatctt ccaccgcgga tgctggcgcg cacgtaatcg
     7321 tatagttcgt gcgagggagc gaggaggtcg ggaccgaggt tgctacgggc gggctgctct
     7381 gctcggaaga ctatctgcct gaagatggca tgtgagttgg atgatatggt tggacgctgg
     7441 aagacgttga agctggcgtc tgtgagacct accgcgtcac gcacgaagga ggcgtaggag
     7501 tcgcgcagct tgttgaccag ctcggcggtg acctgcacgt ctagggcgca gtagtccagg
     7561 gtttccttga tgatgtcata cttatcctgt cccttttttt tccacagctc gcggttgagg
     7621 acaaactctt cgcggtcttt ccagtactct tggatcggaa acccgtcggc ctccgaacgg
     7681 taagagccta gcatgtagaa ctggttgacg gcctggtagg cgcagcatcc cttttctacg
     7741 ggtagcgcgt atgcctgcgc ggccttccgg agcgaggtgt gggtgagcgc aaaggtgtcc
     7801 ctgaccatga ccagcatgaa gggcacgagc tgcttcccaa aggcccccat ccaagtatag
     7861 gtctctacat cgtaggtgac aaagagacgc tcggtgcgag gatgcgagcc gatcgggaag
     7921 aactggatct cccgccacca attggaggag tggctattga tgtggtgaaa gtagaagtcc
     7981 ctgcgacggg ccgaacactc gtgctggctt ttgtaaaaac gtgcgcagta ctggcagcgg
     8041 tgcacgggct gtacatcctg cacgaggttg acctgacgac cgcgcacaag gaagcagagt
     8101 gggaatttga gcccctcgcc tggcgggttt ggctggtggt cttctacttc ggctgcttgt
     8161 ccttgaccgt ctggctgctc gaggggagtt acggtggatc ggaccaccac gccgcgcgag
     8221 cccaaagtcc agatgtccgc gcgcggcggt cggagcttga tgacaacatc gcgcagatgg
     8281 gagctgtcca tggtctggag ctcccgcggc gtcaggtcag gcgggagctc ctgcaggttt
     8341 acctcgcata gacgggtcag ggcgcgggct agatccaggt gatacctaat ttccaggggc
     8401 tggttggtgg cggcgtcgat ggcttgcaag aggccgcatc cccgcggcgc gactacggta
     8461 ccgcgcggcg ggcggtgggc cgcgggggtg tccttggatg atgcatctaa aagcggtgac
     8521 gcgggcgagc ccccggaggt agggggggct ccggacccgc cgggagaggg ggcaggggca
     8581 cgtcggcgcc gcgcgcgggc aggagctggt gctgcgcgcg taggttgctg gcgaacgcga
     8641 cgacgcggcg gttgatctcc tgaatctggc gcctctgcgt gaagacgacg ggcccggtga
     8701 gcttgaacct gaaagagagt tcgacagaat caatttcggt gtcgttgacg gcggcctggc
     8761 gcaaaatctc ctgcacgtct cctgagttgt cttgataggc gatctcggcc atgaactgct
     8821 cgatctcttc ctcctggaga tctccgcgtc cggctcgctc cacggtggcg gcgaggtcgt
     8881 tggaaatgcg ggccatgagc tgcgagaagg cgttgaggcc tccctcgttc cagacgcggc
     8941 tgtagaccac gcccccttcg gcatcgcggg cgcgcatgac cacctgcgcg agattgagct
     9001 ccacgtgccg ggcgaagacg gcgtagtttc gcaggcgctg aaagaggtag ttgagggtgg
     9061 tggcggtgtg ttctgccacg aagaagtaca taacccagcg tcgcaacgtg gattcgttga
     9121 tatcccccaa ggcctcaagg cgctccatgg cctcgtagaa gtccacggcg aagttgaaaa
     9181 actgggagtt gcgcgccgac acggttaact cctcctccag aagacggatg agctcggcga
     9241 cagtgtcgcg cacctcgcgc tcaaaggcta caggggcctc ttcttcttct tcaatctcct
     9301 cttccataag ggcctcccct tcttcttctt ctggcggcgg tgggggaggg gggacacggc
     9361 ggcgacgacg gcgcaccggg aggcggtcga caaagcgctc gatcatctcc ccgcggcgac
     9421 ggcgcatggt ctcggtgacg gcgcggccgt tctcgcgggg gcgcagttgg aagacgccgc
     9481 ccgtcatgtc ccggttatgg gttggcgggg ggctgccatg cggcagggat acggcgctaa
     9541 cgatgcatct caacaattgt tgtgtaggta ctccgccgcc gagggacctg agcgagtccg
     9601 catcgaccgg atcggaaaac ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta
     9661 ggctgagcac cgtggcgggc ggcagcgggc ggcggtcggg gttgtttctg gcggaggtgc
     9721 tgctgatgat gtaattaaag taggcggtct tgagacggcg gatggtcgac agaagcacca
     9781 tgtccttggg tccggcctgc tgaatgcgca ggcggtcggc catgccccag gcttcgtttt
     9841 gacatcggcg caggtctttg tagtagtctt gcatgagcct ttctaccggc acttcttctt
     9901 ctccttcctc ttgtcctgca tctcttgcat ctatcgctgc ggcggcggcg gagtttggcc
     9961 gtaggtggcg ccctcttcct cccatgcgtg tgaccccgaa gcccctcatc ggctgaagca
    10021 gggctaggtc ggcgacaacg cgctcggcta atatggcctg ctgcacctgc gtgagggtag
    10081 actggaagtc atccatgtcc acaaagcggt ggtatgcgcc cgtgttgatg gtgtaagtgc
    10141 agttggccat aacggaccag ttaacggtct ggtgacccgg ctgcgagagc tcggtgtacc
    10201 tgagacgcga gtaagccctc gagtcaaata cgtagtcgtt gcaagtccgc accaggtact
    10261 ggtatcccac caaaaagtgc ggcggcggct ggcggtagag gggccagcgt agggtggccg
    10321 gggctccggg ggcgagatct tccaacataa ggcgatgata tccgtagatg tacctggaca
    10381 tccaggtgat gccggcggcg gtggtggagg cgcgcggaaa gtcgcggacg cggttccaga
    10441 tgttgcgcag cggcaaaaag tgctccatgg tcgggacgct ctggccggtc aggcgcgcgc
    10501 aatcgttgac gctctagacc gtgcaaaagg agagcctgta agcgggcact cttccgtggt
    10561 ctggtggata aattcgcaag ggtatcatgg cggacgaccg gggttcgagc cccgtatccg
    10621 gccgtccgcc gtgatccatg cggttaccgc ccgcgtgtcg aacccaggtg tgcgacgtca
    10681 gacaacgggg gagtgctcct tttggcttcc ttccaggcgc ggcggctgct gcgctagctt
    10741 ttttggccac tggccgcgcg cagcgtaagc ggttaggctg gaaagcgaaa gcattaagtg
    10801 gctcgctccc tgtagccgga gggttatttt ccaagggttg agtcgcggga cccccggttc
    10861 gagtctcgga ccggccggac tgcggcgaac gggggtttgc ctccccgtca tgcaagaccc
    10921 cgcttgcaaa ttcctccgga aacagggacg agcccctttt ttgcttttcc cagatgcatc
    10981 cggtgctgcg gcagatgcgc ccccctcctc agcagcggca agagcaagag cagcggcaga
    11041 catgcagggc accctcccct cctcctaccg cgtcaggagg ggcgacatcc gcggttgacg
    11101 cggcagcaga tggtgattac gaacccccgc ggcgccgggc ccggcactac ctggacttgg
    11161 aggagggcga gggcctggcg cggctaggag cgccctctcc tgagcggcac ccaagggtgc
    11221 agctgaagcg tgatacgcgt gaggcgtacg tgccgcggca gaacctgttt cgcgaccgcg
    11281 agggagagga gcccgaggag atgcgggatc gaaagttcca cgcagggcgc gagctgcggc
    11341 atggcctgaa tcgcgagcgg ttgctgcgcg aggaggactt tgagcccgac gcgcgaaccg
    11401 ggattagtcc cgcgcgcgca cacgtggcgg ccgccgacct ggtaaccgca tacgagcaga
    11461 cggtgaacca ggagattaac tttcaaaaaa gctttaacaa ccacgtgcgt acgcttgtgg
    11521 cgcgcgagga ggtggctata ggactgatgc atctgtggga ctttgtaagc gcgctggagc
    11581 aaaacccaaa tagcaagccg ctcatggcgc agctgttcct tatagtgcag cacagcaggg
    11641 acaacgaggc attcagggat gcgctgctaa acatagtaga gcccgagggc cgctggctgc
    11701 tcgatttgat aaacatcctg cagagcatag tggtgcagga gcgcagcttg agcctggctg
    11761 acaaggtggc cgccatcaac tattccatgc ttagcctggg caagttttac gcccgcaaga
    11821 tataccatac cccttacgtt cccatagaca aggaggtaaa gatcgagggg ttctacatgc
    11881 gcatggcgct gaaggtgctt accttgagcg acgacctggg cgtttatcgc aacgagcgca
    11941 tccacaaggc cgtgagcgtg agccggcggc gcgagctcag cgaccgcgag ctgatgcaca
    12001 gcctgcaaag ggccctggct ggcacgggca gcggcgatag agaggccgag tcctactttg
    12061 acgcgggcgc tgacctgcgc tgggccccaa gccgacgcgc cctggaggca gctggggccg
    12121 gacctgggct ggcggtggca cccgcgcgcg ctggcaacgt cggcggcgtg gaggaatatg
    12181 acgaggacga tgagtacgag ccagaggacg gcgagtacta agcggtgatg tttctgatca
    12241 gatgatgcaa gacgcaacgg acccggcggt gcgggcggcg ctgcagagcc agccgtccgg
    12301 ccttaactcc acggacgact ggcgccaggt catggaccgc atcatgtcgc tgactgcgcg
    12361 caatcctgac gcgttccggc agcagccgca ggccaaccgg ctctccgcaa ttctggaagc
    12421 ggtggtcccg gcgcgcgcaa accccacgca cgagaaggtg ctggcgatcg taaacgcgct
    12481 ggccgaaaac agggccatcc ggcccgacga ggccggcctg gtctacgacg cgctgcttca
    12541 gcgcgtggct cgttacaaca gcggcaacgt gcagaccaac ctggaccggc tggtggggga
    12601 tgtgcgcgag gccgtggcgc agcgtgagcg cgcgcagcag cagggcaacc tgggctccat
    12661 ggttgcacta aacgccttcc tgagtacaca gcccgccaac gtgccgcggg gacaggagga
    12721 ctacaccaac tttgtgagcg cactgcggct aatggtgact gagacaccgc aaagtgaggt
    12781 gtaccagtct gggccagact attttttcca gaccagtaga caaggcctgc agaccgtaaa
    12841 cctgagccag gctttcaaaa acttgcaggg gctgtggggg gtgcgggctc ccacaggcga
    12901 ccgcgcgacc gtgtctagct tgctgacgcc caactcgcgc ctgttgctgc tgctaatagc
    12961 gcccttcacg gacagtggca gcgtgtcccg ggacacatac ctaggtcact tgctgacact
    13021 gtaccgcgag gccataggtc aggcgcatgt ggacgagcat actttccagg agattacaag
    13081 tgtcagccgc gcgctggggc aggaggacac gggcagcctg gaggcaaccc taaactacct
    13141 gctgaccaac cggcggcaga agatcccctc gttgcacagt ttaaacagcg aggaggagcg
    13201 cattttgcgc tacgtgcagc agagcgtgag ccttaacctg atgcgcgacg gggtaacgcc
    13261 cagcgtggcg ctggacatga ccgcgcgcaa catggaaccg ggcatgtatg cctcaaaccg
    13321 gccgtttatc aaccgcctaa tggactactt gcatcgcgcg gccgccgtga accccgagta
    13381 tttcaccaat gccatcttga acccgcactg gctaccgccc cctggtttct acaccggggg
    13441 attcgaggtg cccgagggta acgatggatt cctctgggac gacatagacg acagcgtgtt
    13501 ttccccgcaa ccgcagaccc tgctagagtt gcaacagcgc gagcaggcag aggcggcgct
    13561 gcgaaaggaa agcttccgca ggccaagcag cttgtccgat ctaggcgctg cggccccgcg
    13621 gtcagatgct agtagcccat ttccaagctt gatagggtct cttaccagca ctcgcaccac
    13681 ccgcccgcgc ctgctgggcg aggaggagta cctaaacaac tcgctgctgc agccgcagcg
    13741 cgaaaaaaac ctgcctccgg catttcccaa caacgggata gagagcctag tggacaagat
    13801 gagtagatgg aagacgtacg cgcaggagca cagggacgtg ccaggcccgc gcccgcccac
    13861 ccgtcgtcaa aggcacgacc gtcagcgggg tctggtgtgg gaggacgatg actcggcaga
    13921 cgacagcagc gtcctggatt tgggagggag tggcaacccg tttgcgcacc ttcgccccag
    13981 gctggggaga atgttttaaa aaaaaaaaaa gcatgatgca aaataaaaaa ctcaccaagg
    14041 ccatggcacc gagcgttggt tttcttgtat tccccttagt atgcggcgcg cggcgatgta
    14101 tgaggaaggt cctcctccct cctacgagag tgtggtgagc gcggcgccag tggcggcggc
    14161 gctgggttct cccttcgatg ctcccctgga cccgccgttt gtgcctccgc ggtacctgcg
    14221 gcctaccggg gggagaaaca gcatccgtta ctctgagttg gcacccctat tcgacaccac
    14281 ccgtgtgtac ctggtggaca acaagtcaac ggatgtggca tccctgaact accagaacga
    14341 ccacagcaac tttctgacca cggtcattca aaacaatgac tacagcccgg gggaggcaag
    14401 cacacagacc atcaatcttg acgaccggtc gcactggggc ggcgacctga aaaccatcct
    14461 gcataccaac atgccaaatg tgaacgagtt catgtttacc aataagttta aggcgcgggt
    14521 gatggtgtcg cgcttgccta ctaaggacaa tcaggtggag ctgaaatacg agtgggtgga
    14581 gttcacgctg cccgagggca actactccga gaccatgacc atagacctta tgaacaacgc
    14641 gatcgtggag cactacttga aagtgggcag acagaacggg gttctggaaa gcgacatcgg
    14701 ggtaaagttt gacacccgca acttcagact ggggtttgac cccgtcactg gtcttgtcat
    14761 gcctggggta tatacaaacg aagccttcca tccagacatc attttgctgc caggatgcgg
    14821 ggtggacttc acccacagcc gcctgagcaa cttgttgggc atccgcaagc ggcaaccctt
    14881 ccaggagggc tttaggatca cctacgatga tctggagggt ggtaacattc ccgcactgtt
    14941 ggatgtggac gcctaccagg cgagcttgaa agatgacacc gaacagggcg ggggtggcgc
    15001 aggcggcagc aacagcagtg gcagcggcgc ggaagagaac tccaacgcgg cagccgcggc
    15061 aatgcagccg gtggaggaca tgaacgatca tgccattcgc ggcgacacct ttgccacacg
    15121 ggctgaggag aagcgcgctg aggccgaagc agcggccgaa gctgccgccc ccgctgcgca
    15181 acccgaggtc gagaagcctc agaagaaacc ggtgatcaaa cccctgacag aggacagcaa
    15241 gaaacgcagt tacaacctaa taagcaatga cagcaccttc acccagtacc gcagctggta
    15301 ccttgcatac aactacggcg accctcagac cggaatccgc tcatggaccc tgctttgcac
    15361 tcctgacgta acctgcggct cggagcaggt ctactggtcg ttgccagaca tgatgcaaga
    15421 ccccgtgacc ttccgctcca cgcgccagat cagcaacttt ccggtggtgg gcgccgagct
    15481 gttgcccgtg cactccaaga gcttctacaa cgaccaggcc gtctactccc aactcatccg
    15541 ccagtttacc tctctgaccc acgtgttcaa tcgctttccc gagaaccaga ttttggcgcg
    15601 cccgccagcc cccaccatca ccaccgtcag tgaaaacgtt cctgctctca cagatcacgg
    15661 gacgctaccg ctgcgcaaca gcatcggagg agtccagcga gtgaccatta ctgacgccag
    15721 acgccgcacc tgcccctacg tttacaaggc cctgggcata gtctcgccgc gcgtcctatc
    15781 gagccgcact ttttgagcaa gcatgtccat ccttatatcg cccagcaata acacaggctg
    15841 gggcctgcgc ttcccaagca agatgtttgg cggggccaag aagcgctccg accaacaccc
    15901 agtgcgcgtg cgcgggcact accgcgcgcc ctggggcgcg cacaaacgcg gccgcactgg
    15961 gcgcaccacc gtcgatgacg ccatcgacgc ggtggtggag gaggcgcgca actacacgcc
    16021 cacgccgcca ccagtgtcca cagtggacgc ggccattcag accgtggtgc gcggagcccg
    16081 gcgctatgct aaaatgaaga gacggcggag gcgcgtagca cgtcgccacc gccgccgacc
    16141 cggcactgcc gcccaacgcg cggcggcggc cctgcttaac cgcgcacgtc gcaccggccg
    16201 acgggcggcc atgcgggccg ctcgaaggct ggccgcgggt attgtcactg tgccccccag
    16261 gtccaggcga cgagcggccg ccgcagcagc cgcggccatt agtgctatga ctcagggtcg
    16321 caggggcaac gtgtattggg tgcgcgactc ggttagcggc ctgcgcgtgc ccgtgcgcac
    16381 ccgccccccg cgcaactaga ttgcaagaaa aaactactta gactcgtact gttgtatgta
    16441 tccagcggcg gcggcgcgca acgaagctat gtccaagcgc aaaatcaaag aagagatgct
    16501 ccaggtcatc gcgccggaga tctatggccc cccgaagaag gaagagcagg attacaagcc
    16561 ccgaaagcta aagcgggtca aaaagaaaaa gaaagatgat gatgatgaac ttgacgacga
    16621 ggtggaactg ctgcacgcta ccgcgcccag gcgacgggta cagtggaaag gtcgacgcgt
    16681 aaaacgtgtt ttgcgacccg gcaccaccgt agtctttacg cccggtgagc gctccacccg
    16741 cacctacaag cgcgtgtatg atgaggtgta cggcgacgag gacctgcttg agcaggccaa
    16801 cgagcgcctc ggggagtttg cctacggaaa gcggcataag gacatgctgg cgttgccgct
    16861 ggacgagggc aacccaacac ctagcctaaa gcccgtaaca ctgcagcagg tgctgcccgc
    16921 gcttgcaccg tccgaagaaa agcgcggcct aaagcgcgag tctggtgact tggcacccac
    16981 cgtgcagctg atggtaccca agcgccagcg actggaagat gtcttggaaa aaatgaccgt
    17041 ggaacctggg ctggagcccg aggtccgcgt gcggccaatc aagcaggtgg cgccgggact
    17101 gggcgtgcag accgtggacg ttcagatacc cactaccagt agcaccagta ttgccaccgc
    17161 cacagagggc atggagacac aaacgtcccc ggttgcctca gcggtggcgg atgccgcggt
    17221 gcaggcggtc gctgcggccg cgtccaagac ctctacggag gtgcaaacgg acccgtggat
    17281 gtttcgcgtt tcagcccccc ggcgcccgcg ccgttcgagg aagtacggcg ccgccagcgc
    17341 gctactgccc gaatatgccc tacatccttc cattgcgcct acccccggct atcgtggcta
    17401 cacctaccgc cccagaagac gagcaactac ccgacgccga accaccactg gaacccgccg
    17461 ccgccgtcgc cgtcgccagc ccgtgctggc cccgatttcc gtgcgcaggg tggctcgcga
    17521 aggaggcagg accctggtgc tgccaacagc gcgctaccac cccagcatcg tttaaaagcc
    17581 ggtctttgtg gttcttgcag atatggccct cacctgccgc ctccgtttcc cggtgccggg
    17641 attccgagga agaatgcacc gtaggagggg catggccggc cacggcctga cgggcggcat
    17701 gcgtcgtgcg caccaccggc ggcggcgcgc gtcgcaccgt cgcatgcgcg gcggtatcct
    17761 gcccctcctt attccactga tcgccgcggc gattggcgcc gtgcccggaa ttgcatccgt
    17821 ggccttgcag gcgcagagac actgattaaa aacaagttgc atgtggaaaa atcaaaataa
    17881 aaagtctgga ctctcacgct cgcttggtcc tgtaactatt ttgtagaatg gaagacatca
    17941 actttgcgtc tctggccccg cgacacggct cgcgcccgtt catgggaaac tggcaagata
    18001 tcggcaccag caatatgagc ggtggcgcct tcagctgggg ctcgctgtgg agcggcatta
    18061 aaaatttcgg ttccaccgtt aagaactatg gcagcaaggc ctggaacagc agcacaggcc
    18121 agatgctgag ggataagttg aaagagcaaa atttccaaca aaaggtggta gatggcctgg
    18181 cctctggcat tagcggggtg gtggacctgg ccaaccaggc agtgcaaaat aagattaaca
    18241 gtaagcttga tccccgccct cccgtagagg agcctccacc ggccgtggag acagtgtctc
    18301 cagaggggcg tggcgaaaag cgtccgcgcc ccgacaggga agaaactctg gtgacgcaaa
    18361 tagacgagcc tccctcgtac gaggaggcac taaagcaagg cctgcccacc acccgtccca
    18421 tcgcgcccat ggctaccgga gtgctgggcc agcacacacc cgtaacgctg gacctgcctc
    18481 cccccgccga cacccagcag aaacctgtgc tgccaggccc gaccgccgtt gttgtaaccc
    18541 gtcctagccg cgcgtccctg cgccgcgccg ccagcggtcc gcgatcgttg cggcccgtag
    18601 ccagtggcaa ctggcaaagc acactgaaca gcatcgtggg tctgggggtg caatccctga
    18661 agcgccgacg atgcttctga tagctaacgt gtcgtatgtg tgtcatgtat gcgtccatgt
    18721 cgccgccaga ggagctgctg agccgccgcg cgcccgcttt ccaagatggc taccccttcg
    18781 atgatgccgc agtggtctta catgcacatc tcgggccagg acgcctcgga gtacctgagc
    18841 cccgggctgg tgcagtttgc ccgcgccacc gagacgtact tcagcctgaa taacaagttt
    18901 agaaacccca cggtggcgcc tacgcacgac gtgaccacag accggtccca gcgtttgacg
    18961 ctgcggttca tccctgtgga ccgtgaggat actgcgtact cgtacaaggc gcggttcacc
    19021 ctagctgtgg gtgataaccg tgtgctggac atggcttcca cgtactttga catccgcggc
    19081 gtgctggaca ggggccctac ttttaagccc tactctggca ctgcctacaa cgccctggct
    19141 cccaagggtg ccccaaatcc ttgcgaatgg gatgaagctg ctactgctct tgaaataaac
    19201 ctagaagaag aggacgatga caacgaagac gaagtagacg agcaagctga gcagcaaaaa
    19261 actcacgtat ttgggcaggc gccttattct ggtataaata ttacaaagga gggtattcaa
    19321 ataggtgtcg aaggtcaaac acctaaatat gccgataaaa catttcaacc tgaacctcaa
    19381 ataggagaat ctcagtggta cgaaacagaa attaatcatg cagctgggag agtcctaaaa
    19441 aagactaccc caatgaaacc atgttacggt tcatatgcaa aacccacaaa tgaaaatgga
    19501 gggcaaggca ttcttgtaaa gcaacaaaat ggaaagctag aaagtcaagt ggaaatgcaa
    19561 tttttctcaa ctactgaggc agccgcaggc aatggtgata acttgactcc taaagtggta
    19621 ttgtacagtg aagatgtaga tatagaaacc ccagacactc atatttctta catgcccact
    19681 attaaggaag gtaactcacg agaactaatg ggccaacaat ctatgcccaa caggcctaat
    19741 tacattgctt ttagggacaa ttttattggt ctaatgtatt acaacagcac gggtaatatg
    19801 ggtgttctgg cgggccaagc atcgcagttg aatgctgttg tagatttgca agacagaaac
    19861 acagagcttt cataccagct tttgcttgat tccattggtg atagaaccag gtacttttct
    19921 atgtggaatc aggctgttga cagctatgat ccagatgtta gaattattga aaatcatgga
    19981 actgaagatg aacttccaaa ttactgcttt ccactgggag gtgtgattaa tacagagact
    20041 cttaccaagg taaaacctaa aacaggtcag gaaaatggat gggaaaaaga tgctacagaa
    20101 ttttcagata aaaatgaaat aagagttgga aataattttg ccatggaaat caatctaaat
    20161 gccaacctgt ggagaaattt cctgtactcc aacatagcgc tgtatttgcc cgacaagcta
    20221 aagtacagtc cttccaacgt aaaaatttct gataacccaa acacctacga ctacatgaac
    20281 aagcgagtgg tggctcccgg gctagtggac tgctacatta accttggagc acgctggtcc
    20341 cttgactata tggacaacgt caacccattt aaccaccacc gcaatgctgg cctgcgctac
    20401 cgctcaatgt tgctgggcaa tggtcgctat gtgcccttcc acatccaggt gcctcagaag
    20461 ttctttgcca ttaaaaacct ccttctcctg ccgggctcat acacctacga gtggaacttc
    20521 aggaaggatg ttaacatggt tctgcagagc tccctaggaa atgacctaag ggttgacgga
    20581 gccagcatta agtttgatag catttgcctt tacgccacct tcttccccat ggcccacaac
    20641 accgcctcca cgcttgaggc catgcttaga aacgacacca acgaccagtc ctttaacgac
    20701 tatctctccg ccgccaacat gctctaccct atacccgcca acgctaccaa cgtgcccata
    20761 tccatcccct cccgcaactg ggcggctttc cgcggctggg ccttcacgcg ccttaagact
    20821 aaggaaaccc catcactggg ctcgggctac gacccttatt acacctactc tggctctata
    20881 ccctacctag atggaacctt ttacctcaac cacaccttta agaaggtggc cattaccttt
    20941 gactcttctg tcagctggcc tggcaatgac cgcctgctta cccccaacga gtttgaaatt
    21001 aagcgctcag ttgacgggga gggttacaac gttgcccagt gtaacatgac caaagactgg
    21061 ttcctggtac aaatgctagc taactataac attggctacc agggcttcta tatcccagag
    21121 agctacaagg accgcatgta ctccttcttt agaaacttcc agcccatgag ccgtcaggtg
    21181 gtggatgata ctaaatacaa ggactaccaa caggtgggca tcctacacca acacaacaac
    21241 tctggatttg ttggctacct tgcccccacc atgcgcgaag gacaggccta ccctgctaac
    21301 ttcccctatc cgcttatagg caagaccgca gttgacagca ttacccagaa aaagtttctt
    21361 tgcgatcgca ccctttggcg catcccattc tccagtaact ttatgtccat gggcgcactc
    21421 acagacctgg gccaaaacct tctctacgcc aactccgccc acgcgctaga catgactttt
    21481 gaggtggatc ccatggacga gcccaccctt ctttatgttt tgtttgaagt ctttgacgtg
    21541 gtccgtgtgc accagccgca ccgcggcgtc atcgaaaccg tgtacctgcg cacgcccttc
    21601 tcggccggca acgccacaac ataaagaagc aagcaacatc aacaacagct gccgccaaat
    21661 aatgtactag agacactttc aataaaggca aatgctttta tttgtacact ctcgggtgat
    21721 tatttacccc cacccttgcc gtctgcgccg tttaaaaatc aaaggggttc tgccgcgcat
    21781 cgctatgcgc cactggcagg gacacgttgc gatactggtg tttagtgctc cacttaaact
    21841 caggcacaac catccgcggc agctcggtga agttttcact ccacaggctg cgcaccatca
    21901 ccaacgcgtt tagcaggtcg ggcgccgata tcttgaagtc gcagttgggg cctccgccct
    21961 gcgcgcgcga gttgcgatac acagggttgc agcactggaa cactatcagc gccgggtggt
    22021 gcacgctggc cagcacgctc ttgtcggaga tcagatccgc gtccaggtcc tccgcgttgc
    22081 tcagggcgaa cggagtcaac tttggtagct gccttcccaa aaagggcgcg tgcccaggct
    22141 ttgagttgca ctcgcaccgt agtggcatca aaaggtgacc gtgcccggtc tgggcgttag
    22201 gatacagcgc ctgcataaaa gccttgatct gcttaaaagc cacctgagcc tttgcgcctt
    22261 cagagaagaa catgccgcaa gacttgccgg aaaactgatt ggccggacag gccgcgtcgt
    22321 gcacgcagca ccttgcgtcg gtgttggaga tctgcaccac atttcggccc caccggttct
    22381 tcacgatctt ggccttgcta gactgctcct tcagcgcgcg ctgcccgttt tcgctcgtca
    22441 catccatttc aatcacgtgc tccttattta tcataatgct tccgtgtaga cacttaagct
    22501 cgccttcgat ctcagcgcag cggtgcagcc acaacgcgca gcccgtgggc tcgtgatgct
    22561 tgtaggtcac ctctgcaaac gactgcaggt acgcctgcag gaatcgcccc atcatcgtca
    22621 caaaggtctt gttgctggtg aaggtcagct gcaacccgcg gtgctcctcg ttcagccagg
    22681 tcttgcatac ggccgccaga gcttccactt ggtcaggcag tagtttgaag ttcgccttta
    22741 gatcgttatc cacgtggtac ttgtccatca gcgcgcgcgc agcctccatg cccttctccc
    22801 acgcagacac gatcggcaca ctcagcgggt tcatcaccgt aatttcactt tccgcttcgc
    22861 tgggctcttc ctcttcctct tgcgtccgca taccacgcgc cactgggtcg tcttcattca
    22921 gccgccgcac tgtgcgctta cctcctttgc catgcttgat tagcaccggt gggttgctga
    22981 aacccaccat ttgtagcgcc acatcttctc tttcttcctc gctgtccacg attacctctg
    23041 gtgatggcgg gcgctcgggc ttgggagaag ggcgcttctt tttcttcttg ggcgcaatgg
    23101 ccaaatccgc cgccgaggtc gatggccgcg ggctgggtgt gcgcggcacc agcgcgtctt
    23161 gtgatgagtc ttcctcgtcc tcggactcga tacgccgcct catccgcttt tttgggggcg
    23221 cccggggagg cggcggcgac ggggacgggg acgacacgtc ctccatggtt gggggacgtc
    23281 gcgccgcacc gcgtccgcgc tcgggggtgg tttcgcgctg ctcctcttcc cgactggcca
    23341 tttccttctc ctataggcag aaaaagatca tggagtcagt cgagaagaag gacagcctaa
    23401 ccgccccctc tgagttcgcc accaccgcct ccaccgatgc cgccaacgcg cctaccacct
    23461 tccccgtcga ggcacccccg cttgaggagg aggaagtgat tatcgagcag gacccaggtt
    23521 ttgtaagcga agacgacgag gaccgctcag taccaacaga ggataaaaag caagaccagg
    23581 acaacgcaga ggcaaacgag gaacaagtcg ggcgggggga cgaaaggcat ggcgactacc
    23641 tagatgtggg agacgacgtg ctgttgaagc atctgcagcg ccagtgcgcc attatctgcg
    23701 acgcgttgca agagcgcagc gatgtgcccc tcgccatagc ggatgtcagc cttgcctacg
    23761 aacgccacct attctcaccg cgcgtacccc ccaaacgcca agaaaacggc acatgcgagc
    23821 ccaacccgcg cctcaacttc taccccgtat ttgccgtgcc agaggtgctt gccacctatc
    23881 acatcttttt ccaaaactgc aagatacccc tatcctgccg tgccaaccgc agccgagcgg
    23941 acaagcagct ggccttgcgg cagggcgctg tcatacctga tatcgcctcg ctcaacgaag
    24001 tgccaaaaat ctttgagggt cttggacgcg acgagaagcg cgcggcaaac gctctgcaac
    24061 aggaaaacag cgaaaatgaa agtcactctg gagtgttggt ggaactcgag ggtgacaacg
    24121 cgcgcctagc cgtactaaaa cgcagcatcg aggtcaccca ctttgcctac ccggcactta
    24181 acctaccccc caaggtcatg agcacagtca tgagtgagct gatcgtgcgc cgtgcgcagc
    24241 ccctggagag ggatgcaaat ttgcaagaac aaacagagga gggcctaccc gcagttggcg
    24301 acgagcagct agcgcgctgg cttcaaacgc gcgagcctgc cgacttggag gagcgacgca
    24361 aactaatgat ggccgcagtg ctcgttaccg tggagcttga gtgcatgcag cggttctttg
    24421 ctgacccgga gatgcagcgc aagctagagg aaacattgca ctacaccttt cgacagggct
    24481 acgtacgcca ggcctgcaag atctccaacg tggagctctg caacctggtc tcctaccttg
    24541 gaattttgca cgaaaaccgc cttgggcaaa acgtgcttca ttccacgctc aagggcgagg
    24601 cgcgccgcga ctacgtccgc gactgcgttt acttatttct atgctacacc tggcagacgg
    24661 ccatgggcgt ttggcagcag tgcttggagg agtgcaacct caaggagctg cagaaactgc
    24721 taaagcaaaa cttgaaggac ctatggacgg ccttcaacga gcgctccgtg gccgcgcacc
    24781 tggcggacat cattttcccc gaacgcctgc ttaaaaccct gcaacagggt ctgccagact
    24841 tcaccagtca aagcatgttg cagaacttta ggaactttat cctagagcgc tcaggaatct
    24901 tgcccgccac ctgctgtgca cttcctagcg actttgtgcc cattaagtac cgcgaatgcc
    24961 ctccgccgct ttggggccac tgctaccttc tgcagctagc caactacctt gcctaccact
    25021 ctgacataat ggaagacgtg agcggtgacg gtctactgga gtgtcactgt cgctgcaacc
    25081 tatgcacccc gcaccgctcc ctggtttgca attcgcagct gcttaacgaa agtcaaatta
    25141 tcggtacctt tgagctgcag ggtccctcgc ctgacgaaaa gtccgcggct ccggggttga
    25201 aactcactcc ggggctgtgg acgtcggctt accttcgcaa atttgtacct gaggactacc
    25261 acgcccacga gattaggttc tacgaagacc aatcccgccc gcctaatgcg gagcttaccg
    25321 cctgcgtcat tacccagggc cacattcttg gccaattgca agccatcaac aaagcccgcc
    25381 aagagtttct gctacgaaag ggacgggggg tttacttgga cccccagtcc ggcgaggagc
    25441 tcaacccaat ccccccgccg ccgcagccct atcagcagca gccgcgggcc cttgcttccc
    25501 aggatggcac ccaaaaagaa gctgcagctg ccgccgccac ccacggacga ggaggaatac
    25561 tgggacagtc aggcagagga ggttttggac gaggaggagg aggacatgat ggaagactgg
    25621 gagagcctag acgaggaagc ttccgaggtc gaagaggtgt cagacgaaac accgtcaccc
    25681 tcggtcgcat tcccctcgcc ggcgccccag aaatcggcaa ccggttccag catggctaca
    25741 acctccgctc ctcaggcgcc gccggcactg cccgttcgcc gacccaaccg tagatgggac
    25801 accactggaa ccagggccgg taagtccaag cagccgccgc cgttagccca agagcaacaa
    25861 cagcgccaag gctaccgctc atggcgcggg cacaagaacg ccatagttgc ttgcttgcaa
    25921 gactgtgggg gcaacatctc cttcgcccgc cgctttcttc tctaccatca cggcgtggcc
    25981 ttcccccgta acatcctgca ttactaccgt catctctaca gcccatactg caccggcggc
    26041 agcggcagca acagcagcgg ccacacagaa gcaaaggcga ccggatagca agactctgac
    26101 aaagcccaag aaatccacag cggcggcagc agcaggagga ggagcgctgc gtctggcgcc
    26161 caacgaaccc gtatcgaccc gcgagcttag aaacaggatt tttcccactc tgtatgctat
    26221 atttcaacag agcaggggcc aagaacaaga gctgaaaata aaaaacaggt ctctgcgatc
    26281 cctcacccgc agctgcctgt atcacaaaag cgaagatcag cttcggcgca cgctggaaga
    26341 cgcggaggct ctcttcagta aatactgcgc gctgactctt aaggactagt ttcgcgccct
    26401 ttctcaaatt taagcgcgaa aactacgtca tctccagcgg ccacacccgg cgccagcacc
    26461 tgttgtcagc gccattatga gcaaggaaat tcccacgccc tacatgtgga gttaccagcc
    26521 acaaatggga cttgcggctg gagctgccca agactactca acccgaataa actacatgag
    26581 cgcgggaccc cacatgatat cccgggtcaa cggaatacgc gcccaccgaa accgaattct
    26641 cctggaacag gcggctatta ccaccacacc tcgtaataac cttaatcccc gtagttggcc
    26701 cgctgccctg gtgtaccagg aaagtcccgc tcccaccact gtggtacttc ccagagacgc
    26761 ccaggccgaa gttcagatga ctaactcagg ggcgcagctt gcgggcggct ttcgtcacag
    26821 ggtgcggtcg cccgggcagg gtataactca cctgacaatc agagggcgag gtattcagct
    26881 caacgacgag tcggtgagct cctcgcttgg tctccgtccg gacgggacat ttcagatcgg
    26941 cggcgccggc cgctcttcat tcacgcctcg tcaggcaatc ctaactctgc agacctcgtc
    27001 ctctgagccg cgctctggag gcattggaac tctgcaattt attgaggagt ttgtgccatc
    27061 ggtctacttt aaccccttct cgggacctcc cggccactat ccggatcaat ttattcctaa
    27121 ctttgacgcg gtaaaggact cggcggacgg ctacgactga atgttaagtg gagaggcaga
    27181 gcaactgcgc ctgaaacacc tggtccactg tcgccgccac aagtgctttg cccgcgactc
    27241 cggtgagttt tgctactttg aattgcccga ggatcatatc gagggcccgg cgcacggcgt
    27301 ccggcttacc gcccagggag agcttgcccg tagcctgatt cgggagttta cccagcgccc
    27361 cctgctagtt gagcgggaca ggggaccctg tgttctcact gtgatttgca actgtcctaa
    27421 ccctggatta catcaagatc tttgttgcca tctctgtgct gagtataata aatacagaaa
    27481 ttaaaatata ctggggctcc tatcgccatc ctgtaaacgc caccgtcttc acccgcccaa
    27541 gcaaaccaag gcgaacctta cctggtactt ttaacatctc tccctctgtg atttacaaca
    27601 gtttcaaccc agacggagtg agtctacgag agaacctctc cgagctcagc tactccatca
    27661 gaaaaaacac caccctcctt acctgccggg aacgtacgag tgcgtcaccg gccgctgcac
    27721 cacacctacc gcctgaccgt aaaccagact ttttccggac agacctcaat aactctgttt
    27781 accagaacag gaggtgagct tagaaaaccc ttagggtatt aggccaaagg cgcagctact
    27841 gtggggttta tgaacaattc aagcaactct acgggctatt ctaattcagg tttctctaga
    27901 aatggacgga attattacag agcagcgcct gctagaaaga cgcagggcag cggccgagca
    27961 acagcgcatg aatcaagagc tccaagacat ggttaacttg caccagtgca aaaggggtat
    28021 cttttgtctg gtaaagcagg ccaaagtcac ctacgacagt aataccaccg gacaccgcct
    28081 tagctacaag ttgccaacca agcgtcagaa attggtggtc atggtgggag aaaagcccat
    28141 taccataact cagcactcgg tagaaaccga aggctgcatt cactcacctt gtcaaggacc
    28201 tgaggatctc tgcaccctta ttaagaccct gtgcggtctc aaagatctta ttccctttaa
    28261 ctaataaaaa aaaataataa agcatcactt acttaaaatc agttagcaaa tttctgtcca
    28321 gtttattcag cagcacctcc ttgccctcct cccagctctg gtattgcagc ttcctcctgg
    28381 ctgcaaactt tctccacaat ctaaatggaa tgtcagtttc ctcctgttcc tgtccatccg
    28441 cacccactat cttcatgttg ttgcagatga agcgcgcaag accgtctgaa gataccttca
    28501 accccgtgta tccatatgac acggaaaccg gtcctccaac tgtgcctttt cttactcctc
    28561 cctttgtatc ccccaatggg tttcaagaga gtccccctgg ggtactctct ttgcgcctat
    28621 ccgaacctct agttacctcc aatggcatgc ttgcgctcaa aatgggcaac ggcctctctc
    28681 tggacgaggc cggcaacctt acctcccaaa atgtaaccac tgtgagccca cctctcaaaa
    28741 aaaccaagtc aaacataaac ctggaaatat ctgcacccct cacagttacc tcagaagccc
    28801 taactgtggc tgccgccgca cctctaatgg tcgcgggcaa cacactcacc atgcaatcac
    28861 aggccccgct aaccgtgcac gactccaaac ttagcattgc cacccaagga cccctcacag
    28921 tgtcagaagg aaagctagcc ctgcaaacat caggccccct caccaccacc gatagcagta
    28981 cccttactat cactgcctca ccccctctaa ctactgccac tggtagcttg ggcattgact
    29041 tgaaagagcc catttataca caaaatggaa aactaggact aaagtacggg gctcctttgc
    29101 atgtaacaga cgacctaaac actttgaccg tagcaactgg tccaggtgtg actattaata
    29161 atacttcctt gcaaactaaa gttactggag ccttgggttt tgattcacaa ggcaatatgc
    29221 aacttaatgt agcaggagga ctaaggattg attctcaaaa cagacgcctt atacttgatg
    29281 ttagttatcc gtttgatgct caaaaccaac taaatctaag actaggacag ggccctcttt
    29341 ttataaactc agcccacaac ttggatatta actacaacaa aggcctttac ttgtttacag
    29401 cttcaaacaa ttccaaaaag cttgaggtta acctaagcac tgccaagggg ttgatgtttg
    29461 acgctacagc catagccatt aatgcaggag atgggcttga atttggttca cctaatgcac
    29521 caaacacaaa tcccctcaaa acaaaaattg gccatggcct agaatttgat tcaaacaagg
    29581 ctatggttcc taaactagga actggcctta gttttgacag cacaggtgcc attacagtag
    29641 gaaacaaaaa taatgataag ctaactttgt ggaccacacc agctccatct cctaactgta
    29701 gactaaatgc agagaaagat gctaaactca ctttggtctt aacaaaatgt ggcagtcaaa
    29761 tacttgctac agtttcagtt ttggctgtta aaggcagttt ggctccaata tctggaacag
    29821 ttcaaagtgc tcatcttatt ataagatttg acgaaaatgg agtgctacta aacaattcct
    29881 tcctggaccc agaatattgg aactttagaa atggagatct tactgaaggc acagcctata
    29941 caaacgctgt tggatttatg cctaacctat cagcttatcc aaaatctcac ggtaaaactg
    30001 ccaaaagtaa cattgtcagt caagtttact taaacggaga caaaactaaa cctgtaacac
    30061 taaccattac actaaacggt acacaggaaa caggagacac aactccaagt gcatactcta
    30121 tgtcattttc atgggactgg tctggccaca actacattaa tgaaatattt gccacatcct
    30181 cttacacttt ttcatacatt gcccaagaat aaagaatcgt ttgtgttatg tttcaacgtg
    30241 tttatttttc aattgcagaa aatttcgaat catttttcat tcagtagtat agccccacca
    30301 ccacatagct tatacagatc accgtacctt aatcaaactc acagaaccct agtattcaac
    30361 ctgccacctc cctcccaaca cacagagtac acagtccttt ctccccggct ggccttaaaa
    30421 agcatcatat catgggtaac agacatattc ttaggtgtta tattccacac ggtttcctgt
    30481 cgagccaaac gctcatcagt gatattaata aactccccgg gcagctcact taagttcatg
    30541 tcgctgtcca gctgctgagc cacaggctgc tgtccaactt gcggttgctt aacgggcggc
    30601 gaaggagaag tccacgccta catgggggta gagtcataat cgtgcatcag gatagggcgg
    30661 tggtgctgca gcagcgcgcg aataaactgc tgccgccgcc gctccgtcct gcaggaatac
    30721 aacatggcag tggtctcctc agcgatgatt cgcaccgccc gcagcataag gcgccttgtc
    30781 ctccgggcac agcagcgcac cctgatctca cttaaatcag cacagtaact gcagcacagc
    30841 accacaatat tgttcaaaat cccacagtgc aaggcgctgt atccaaagct catggcgggg
    30901 accacagaac ccacgtggcc atcataccac aagcgcaggt agattaagtg gcgacccctc
    30961 ataaacacgc tggacataaa cattacctct tttggcatgt tgtaattcac cacctcccgg
    31021 taccatataa acctctgatt aaacatggcg ccatccacca ccatcctaaa ccagctggcc
    31081 aaaacctgcc cgccggctat acactgcagg gaaccgggac tggaacaatg acagtggaga
    31141 gcccaggact cgtaaccatg gatcatcatg ctcgtcatga tatcaatgtt ggcacaacac
    31201 aggcacacgt gcatacactt cctcaggatt acaagctcct cccgcgttag aaccatatcc
    31261 cagggaacaa cccattcctg aatcagcgta aatcccacac tgcagggaag acctcgcacg
    31321 taactcacgt tgtgcattgt caaagtgtta cattcgggca gcagcggatg atcctccagt
    31381 atggtagcgc gggtttctgt ctcaaaagga ggtagacgat ccctactgta cggagtgcgc
    31441 cgagacaacc gagatcgtgt tggtcgtagt gtcatgccaa atggaacgcc ggacgtagtc
    31501 atatttcctg aagcaaaacc aggtgcgggc gtgacaaaca gatctgcgtc tccggtctcg
    31561 ccgcttagat cgctctgtgt agtagttgta gtatatccac tctctcaaag catccaggcg
    31621 ccccctggct tcgggttcta tgtaaactcc ttcatgcgcc gctgccctga taacatccac
    31681 caccgcagaa taagccacac ccagccaacc tacacattcg ttctgcgagt cacacacggg
    31741 aggagcggga agagctggaa gaaccatgtt ttttttttta ttccaaaaga ttatccaaaa
    31801 cctcaaaatg aagatctatt aagtgaacgc gctcccctcc ggtggcgtgg tcaaactcta
    31861 cagccaaaga acagataatg gcatttgtaa gatgttgcac aatggcttcc aaaaggcaaa
    31921 cggccctcac gtccaagtgg acgtaaaggc taaacccttc agggtgaatc tcctctataa
    31981 acattccagc accttcaacc atgcccaaat aattctcatc tcgccacctt ctcaatatat
    32041 ctctaagcaa atcccgaata ttaagtccgg ccattgtaaa aatctgctcc agagcgccct
    32101 ccaccttcag cctcaagcag cgaatcatga ttgcaaaaat tcaggttcct cacagacctg
    32161 tataagattc aaaagcggaa cattaacaaa aataccgcga tcccgtaggt cccttcgcag
    32221 ggccagctga acataatcgt gcaggtctgc acggaccagc gcggccactt ccccgccagg
    32281 aaccatgaca aaagaaccca cactgattat gacacgcata ctcggagcta tgctaaccag
    32341 cgtagccccg atgtaagctt gttgcatggg cggcgatata aaatgcaagg tgctgctcaa
    32401 aaaatcaggc aaagcctcgc gcaaaaaaga aagcacatcg tagtcatgct catgcagata
    32461 aaggcaggta agctccggaa ccaccacaga aaaagacacc atttttctct caaacatgtc
    32521 tgcgggtttc tgcataaaca caaaataaaa taacaaaaaa acatttaaac attagaagcc
    32581 tgtcttacaa caggaaaaac aacccttata agcataagac ggactacggc catgccggcg
    32641 tgaccgtaaa aaaactggtc accgtgatta aaaagcacca ccgacagctc ctcggtcatg
    32701 tccggagtca taatgtaaga ctcggtaaac acatcaggtt gattcacatc ggtcagtgct
    32761 aaaaagcgac cgaaatagcc cgggggaata catacccgca ggcgtagaga caacattaca
    32821 gcccccatag gaggtataac aaaattaata ggagagaaaa acacataaac acctgaaaaa
    32881 ccctcctgcc taggcaaaat agcaccctcc cgctccagaa caacatacag cgcttccaca
    32941 gcggcagcca taacagtcag ccttaccagt aaaaaagaaa acctattaaa aaaacaccac
    33001 tcgacacggc accagctcaa tcagtcacag tgtaaaaaag ggccaagtgc agagcgagta
    33061 tatataggac taaaaaatga cgtaacggtt aaagtccaca aaaaacaccc agaaaaccgc
    33121 acgcgaacct acgcccagaa acgaaagcca aaaaacccac aacttcctca aatcgtcact
    33181 tccgttttcc cacgttactt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
    33241 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
    33301 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
    33361 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
    33421 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
    33481 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaac
    33541 ctccgcgggg atccgcacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
    33601 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
    33661 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
    33721 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
    33781 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
    33841 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
    33901 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
    33961 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
    34021 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
    34081 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
    34141 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
    34201 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
    34261 gtacaagtag ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
    34321 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
    34381 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
    34441 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    34501 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    34561 tataccgtcg acctctagct agaggtcact tcccatttta agaaaactac aattcccaac
    34621 acatacaagt tactccgccc taaaacctac gtcacccgcc ccgttcccac gccccgcgcc
    34681 acgtcacaaa ctccaccccc tcattatcat attggcttca atccaaaata aggtatatta
    34741 ttgatgatgt taattaattt aaatccgcat gcgatatcga gctctcccgg gaattcggat
    34801 ctgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg
    34861 ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc
    34921 ttcaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    34981 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    35041 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    35101 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    35161 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    35221 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    35281 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    35341 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    35401 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    35461 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    35521 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    35581 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    35641 tatcaaaaag gatcttcacc tagatccttt taaatcaatc taaagtatat atgagtaaac
    35701 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    35761 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    35821 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    35881 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    35941 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    36001 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    36061 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    36121 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    36181 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    36241 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    36301 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
    36361 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    36421 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    36481 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    36541 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    36601 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    36661 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    36721 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
    36781 attggatccg aattcccggg agagctcgat atcgcatgcg gatttaaatt aattaa
//

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