kiCAP-AAV-BI-hTFR1

LOCUS       kiCAP-AAV-BI-hTFR1       9330 bp DNA     circular SYN 25-AUG-2024
DEFINITION  RepCap for AAV production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9330)
  AUTHORS   Huang Q, Chan KY, Lou S, Keyes C, Wu J, Botticello-Romero NR, Zheng 
            Q, Johnston J, Mills A, Brauer PP, Clouse G, Pacouret S, Harvey JW, 
            Beddow T, Hurley JK, Tobey IG, Powell M, Chen AT, Barry AJ, Eid FE, 
            Chan YA, Deverman BE
  TITLE     An AAV capsid reprogrammed to bind human Transferrin Receptor 
            mediates brain-wide gene delivery.
  JOURNAL   bioRxiv [Preprint]. 2023 Dec 22:2023.12.20.572615. doi: 
            10.1101/2023.12.20.572615.
  PUBMED    38187643
REFERENCE   2  (bases 1 to 9330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2024 from SnapGene Server 7.0.3
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Dec 22:2023.12.20.572615. doi: 
            10.1101/2023.12.20.572615."
FEATURES             Location/Qualifiers
     source          1..9330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2577..2687
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     promoter        complement(2726..2744)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2727..2744)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2764..2781)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2770..2994
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3056..3074
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3093..3111
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3130..3148
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3167..3185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3204..3222
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3241..3259
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3278..3296
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3315..3333
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             6050..6061
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(6513..6533)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6513..6531)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6552..6568)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6552..6568)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6565..6587)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6576..6592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6600..6630)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6645..6666
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6783..6800)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6954..7542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7034..7053)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7716..8525)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8439..8458
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(8526..8630)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8656..9111)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8793..8814)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9005..9024
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9238..9260
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9252..9269
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9253..9269
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9279..9298
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9279..9297
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     join(9326..9330,1..12)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     join(9327..9330,1..13)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgacggta tcgggggagc tcgcagggtc tccattttga agcgggaggt ttgaacgcgc
       61 agccgccatg ccggggtttt acgagattgt gattaaggtc cccagcgacc ttgacgagca
      121 tctgcccggc atttctgaca gctttgtgaa ctgggtggcc gagaaggaat gggagttgcc
      181 gccagattct gacatggatc tgaatctgat tgagcaggca cccctgaccg tggccgagaa
      241 gctgcagcgc gactttctga cggaatggcg ccgtgtgagt aaggccccgg aggctctttt
      301 ctttgtgcaa tttgagaagg gagagagcta cttccacatg cacgtgctcg tggaaaccac
      361 cggggtgaaa tccatggttt tgggacgttt cctgagtcag attcgcgaaa aactgattca
      421 gagaatttac cgcgggatcg agccgacttt gccaaactgg ttcgcggtca caaagaccag
      481 aaatggcgcc ggaggcggga acaaggtggt ggatgagtgc tacatcccca attacttgct
      541 ccccaaaacc cagcctgagc tccagtgggc gtggactaat atggaacagt atttaagcgc
      601 ctgtttgaat ctcacggagc gtaaacggtt ggtggcgcag catctgacgc acgtgtcgca
      661 gacgcaggag cagaacaaag agaatcagaa tcccaattct gatgcgccgg tgatcagatc
      721 aaaaacttca gccaggtaca tggagctggt cgggtggctc gtggacaagg ggattacctc
      781 ggagaagcag tggatccagg aggaccaggc ctcatacatc tccttcaatg cggcctccaa
      841 ctcgcggtcc caaatcaagg ctgccttgga caatgcggga aagattatga gcctgactaa
      901 aaccgccccc gactacctgg tgggccagca gcccgtggag gacatttcca gcaatcggat
      961 ttataaaatt ttggaactaa acgggtacga tccccaatat gcggcttccg tctttctggg
     1021 atgggccacg aaaaagttcg gcaagaggaa caccatctgg ctgtttgggc ctgcaactac
     1081 cgggaagacc aacatcgcgg aggccatagc ccacactgtg cccttctacg ggtgcgtaaa
     1141 ctggaccaat gagaactttc ccttcaacga ctgtgtcgac aagatggtga tctggtggga
     1201 ggaggggaag atgaccgcca aggtcgtgga gtcggccaaa gccattctcg gaggaagcaa
     1261 ggtgcgcgtg gaccagaaat gcaagtcctc ggcccagata gacccgactc ccgtgatcgt
     1321 cacctccaac accaacatgt gcgccgtgat tgacgggaac tcaacgacct tcgaacacca
     1381 gcagccgttg caagaccgga tgttcaaatt tgaactcacc cgccgtctgg atcatgactt
     1441 tgggaaggtc accaagcagg aagtcaaaga ctttttccgg tgggcaaagg atcacgtggt
     1501 tgaggtggag catgaattct acgtcaaaaa gggtggagcc aagaaaagac ccgcccccag
     1561 tgacgcagat ataagtgagc ccaaacgggt gcgcgagtca gttgcgcagc catcgacgtc
     1621 agacgcggaa gcttcgatca actacgcgga caggtaccaa aacaaatgtt ctcgtcacgt
     1681 gggcatgaat ctgatgctgt ttccctgcag acaatgcgag agactgaatc agaattcaaa
     1741 tatctgcttc actcacggtg tcaaagactg tttagagtgc tttcccgtgt cagaatctca
     1801 acccgtttct gtcgtcaaaa aggcgtatca gaaactgtgc tacattcatc acatcatggg
     1861 aaaggtgcca gacgcttgca ctgcttgcga cctggtcaat gtggacttgg atgactgtgt
     1921 ttctgaacaa taaatgactt aaaccaggta tgagtcggct ggataaatct aaagtcataa
     1981 acggcgctct ggaattactc aatgaagtcg gtatcgaagg cctgacgaca aggaaactcg
     2041 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
     2101 tgctcgatgc cctggccatc gagatgctgg acaggcatca tacccacttc tgccccctgg
     2161 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcattccgc tgtgctctcc
     2221 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg
     2281 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac
     2341 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc
     2401 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga
     2461 gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg
     2521 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccggccgacg
     2581 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg
     2641 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaaatgc
     2701 atgaattcga tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
     2761 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     2821 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     2881 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     2941 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     3001 aggcggaaag aaccagctgg ggctcgaatc aagctatcaa gtgccacctg acgtctccct
     3061 atcagtgata gagaagtcga cacgtctcga gctccctatc agtgatagag aaggtacgtc
     3121 tagaacgtct ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat
     3181 agagaaggta cgtctagaac gtctccctat cagtgataga gaagtcgaca cgtctcgagc
     3241 tccctatcag tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc
     3301 gacacgtctc gagctcccta tcagtgatag agaaggtacc ccctatataa gcagagagat
     3361 ctgttcaaat ttgaactgac taagcggctc ccgccagatt ttggcaagat tactaagcag
     3421 gaagtcaagg acttttttgc ttgggcaaag gtcaatcagg tgccggtgac tcacgagttt
     3481 aaagttccca gggaattggc gggaactaaa ggggcggaga aatctctaaa acgcccactg
     3541 ggtgacgtca ccaatactag ctataaaagt ctggagaagc gggccaggct ctcatttgtt
     3601 cccgagacgc ctcgcagttc agacgtgact gttgatcccg ctcctctgcg accgctagct
     3661 tcgatcaact acgcggacag gtaccaaaac aaatgttctc gtcacgtggg catgaatctg
     3721 atgctgtttc cctgcagaca atgcgagaga ctgaatcaga attcaaatat ctgcttcact
     3781 cacggtgtca aagactgttt agagtgcttt cccgtgtcag aatctcaacc cgtttctgtc
     3841 gtcaaaaagg cgtatcagaa actgtgctac attcatcaca tcatgggaaa ggtgccagac
     3901 gcttgcactg cttgcgacct ggtcaatgtg gacttggatg actgtgtttc tgaacaataa
     3961 atgacttaaa ccaggtatgg ctgccgatgg ttatcttcca gattggctcg aggacaacct
     4021 tagtgaagga attcgcgagt ggtgggcttt gaaacctgga gcccctcaac ccaaggcaaa
     4081 tcaacaacat caagacaacg ctcgaggtct tgtgcttccg ggttacaaat accttggacc
     4141 cggcaacgga ctcgacaagg gggagccggt caacgcagca gacgcggcgg ccctcgagca
     4201 cgacaaggcc tacgaccagc agctcaaggc cggagacaac ccgtacctca agtacaacca
     4261 cgccgacgcc gagttccagg agcggctcaa agaagatacg tcttttgggg gcaacctcgg
     4321 gcgagcagtc ttccaggcca aaaagaggct tcttgaacct cttggtctgg ttgaggaagc
     4381 ggctaagacg gctcctggaa agaagaggcc tgtagagcag tctcctcagg aaccggactc
     4441 ctccgcgggt attggcaaat cgggtgcaca gcccgctaaa aagagactca atttcggtca
     4501 gactggcgac acagagtcag tcccagaccc tcaaccaatc ggagaacctc ccgcagcccc
     4561 ctcaggtgtg ggatctctta caatggcttc aggtggtggc gcaccagtgg cagacaataa
     4621 cgaaggtgcc gatggagtgg gtagttcctc gggaaattgg cattgcgatt cccaatggct
     4681 gggggacaga gtcatcacca ccagcacccg aacctgggcc ctgcccacct acaacaatca
     4741 cctctacaag caaatctcca acagcacatc tggaggatct tcaaatgaca acgcctactt
     4801 cggctacagc accccctggg ggtattttga cttcaacaga ttccactgcc acttctcacc
     4861 acgtgactgg cagcgactca tcaacaacaa ctggggattc cggcctaagc gactcaactt
     4921 caagctcttc aacattcagg tcaaagaggt tacggacaac aatggagtca agaccatcgc
     4981 caataacctt accagcacgg tccaggtctt cacggactca gactatcagc tcccgtacgt
     5041 gctcgggtcg gctcacgagg gctgcctccc gccgttccca gcggacgttt tcatgattcc
     5101 tcagtacggg tatctgacgc ttaatgatgg aagccaggcc gtgggtcgtt cgtcctttta
     5161 ctgcctggaa tatttcccgt cgcaaatgct aagaacgggt aacaacttcc agttcagcta
     5221 cgagtttgag aacgtacctt tccatagcag ctacgctcac agccaaagcc tggaccgact
     5281 aatgaatcca ctcatcgacc aatacttgta ctatctctct agaactatta acggttctgg
     5341 acagaatcaa caaacgctaa aattcagtgt ggccggaccc agcaacatgg ctgtccaggg
     5401 aagaaactac atacctggac ccagctaccg acaacaacgt gtctcaacca ctgtgactca
     5461 aaacaacaac agcgaatttg cttggcctgg agcttcttct tgggctctca atggacgtaa
     5521 tagcttgatg aatcctggac ctgctatggc cagccacaaa gaaggagagg accgtttctt
     5581 tcctttgtct ggatctttaa tttttggcaa acaaggaact ggaagagaca acgtggatgc
     5641 ggacaaagtc atgataacca acgaagaaga aattaaaact actaacccgg tagcaacgga
     5701 gtcctatgga caagtggcca caaaccacca gagtgcccaa tactcacgca tcggccccaa
     5761 cgcacaggcg cagaccggtt gggttcaaaa ccaaggaata cttccgggta tggtttggca
     5821 ggacagagat gtgtacctgc aaggacccat ttgggccaaa attcctcaca cggacggcaa
     5881 ctttcaccct tctccgctga tgggagggtt tggaatgaag cacccgcctc ctcagatcct
     5941 catcaaaaac acacctgtac ctgcggatcc tccaacggcc ttcaacaagg acaagctgaa
     6001 ctctttcatc acccagtatt ctactggcca agtcagcgtg gagatcgagt gggagctgca
     6061 gaaggaaaac agcaagcgct ggaacccgga gatccagtac acttccaact attacaagtc
     6121 taataatgtt gaatttgctg ttaatactga aggtgtatat agtgaacccc gccccattgg
     6181 caccagatac ctgactcgta atctgtaatt gcttgttaat caataaaccg tttaattcgt
     6241 ttcagttgaa ctttggtctc tgcgaagggc gaattcgttt aaacctgcag gactagaggt
     6301 cctgtattag aggtcacgtg agtgttttgc gacattttgc gacaccatgt ggtcacgctg
     6361 ggtatttaag cccgagtgag cacgcagggt ctccattttg aagcgggagg tttgaacgcg
     6421 cagccgccaa gccgaattct gcagatatcc atcacactgg cggccgctcg actagagcgg
     6481 ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     6541 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6601 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6661 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6721 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6781 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6841 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6901 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6961 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7021 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7081 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7141 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7201 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7261 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7321 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7381 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7441 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7501 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7561 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7621 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7681 taaagtatat atgagtaaac ttggtctgac agttattaga aaaactcatc gagcatcaaa
     7741 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     7801 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     7861 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     7921 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt
     7981 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     8041 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     8101 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     8161 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     8221 tttccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     8281 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     8341 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     8401 tacaagcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     8461 tataaatcag catccatgtt ggaatttaat cgcggcctcg acgtttcccg ttgaatatgg
     8521 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8581 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8641 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     8701 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     8761 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     8821 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     8881 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     8941 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     9001 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     9061 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
     9121 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     9181 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     9241 agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg
     9301 aattgggtac cgggcccccc ctcgatcgag
//